全文获取类型
收费全文 | 17697篇 |
免费 | 3864篇 |
国内免费 | 111篇 |
专业分类
林业 | 1214篇 |
农学 | 897篇 |
基础科学 | 100篇 |
4272篇 | |
综合类 | 538篇 |
农作物 | 731篇 |
水产渔业 | 3345篇 |
畜牧兽医 | 8151篇 |
园艺 | 240篇 |
植物保护 | 2184篇 |
出版年
2023年 | 97篇 |
2022年 | 152篇 |
2021年 | 442篇 |
2020年 | 761篇 |
2019年 | 1251篇 |
2018年 | 1322篇 |
2017年 | 1366篇 |
2016年 | 1355篇 |
2015年 | 1130篇 |
2014年 | 1215篇 |
2013年 | 1626篇 |
2012年 | 1366篇 |
2011年 | 1325篇 |
2010年 | 1097篇 |
2009年 | 690篇 |
2008年 | 875篇 |
2007年 | 739篇 |
2006年 | 657篇 |
2005年 | 643篇 |
2004年 | 568篇 |
2003年 | 522篇 |
2002年 | 445篇 |
2001年 | 298篇 |
2000年 | 327篇 |
1999年 | 211篇 |
1998年 | 55篇 |
1997年 | 70篇 |
1996年 | 44篇 |
1995年 | 42篇 |
1994年 | 29篇 |
1993年 | 31篇 |
1992年 | 63篇 |
1991年 | 47篇 |
1990年 | 57篇 |
1989年 | 58篇 |
1988年 | 43篇 |
1987年 | 46篇 |
1986年 | 51篇 |
1985年 | 54篇 |
1984年 | 42篇 |
1983年 | 37篇 |
1982年 | 23篇 |
1981年 | 17篇 |
1979年 | 37篇 |
1978年 | 37篇 |
1977年 | 33篇 |
1974年 | 29篇 |
1973年 | 47篇 |
1972年 | 34篇 |
1971年 | 27篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
961.
Miguel A Hurtado Ilie S Racotta Olivia Arjona Mónica Hernández-Rodríguez Ernesto Goytortúa Roberto Civera & Elena Palacios 《Aquaculture Research》2006,37(13):1316-1326
Litopenaeus vannamei (Boone) grown in ponds are exposed to salinities of less than 5 g L?1 during inland shrimp culture or to more than 40 g L?1 from evaporation and reduced water exchange in dry, hot climates. However, dietary requirements for shrimp grown in low or high salinities are not well defined, particularly for fatty acids. Feeding shrimp postlarvae with highly unsaturated fatty acids (HUFA) enhances tolerance to acute exposure to low salinity, as a result of better nutritional status, or/and specific effects of HUFA on membrane function and osmoregulation mechanisms. This study analysed the effect of HUFA supplementation (3% vs. 34%) on L. vannamei juveniles reared for 21 days at low (5 g L?1), medium (30 g L?1) and high salinities (50 g L?1). Juveniles grown at 5 g L?1 had lower survival compared with controls (30 g L?1) or shrimp grown at 50 g L?1, but no significant effect on survival was observed as a result of HUFA enrichment. In contrast, growth was significantly lower for shrimp grown at 50 g L?1, but this effect was compensated by the HUFA‐enriched diet. Osmotic pressure in haemolymph was affected by salinity, but not by HUFA enrichment. Shrimp fed HUFA‐enriched diets had significantly higher levels of eicosapentaenoic acid and docosahexaenoic acid in hepatopancreas and gills. These results demonstrate that growth at high salinities is enhanced with diets containing high HUFA levels, but that HUFA‐enriched diets have no effect on shrimp reared at low salinities. 相似文献
962.
Francisco J Magallón Barajas Rosalía Servín Villegas Guillermo Portillo Clark & Berenice López Moreno 《Aquaculture Research》2006,37(5):492-499
Transport of post‐larvae shrimp used in aquaculture is an important element of successful cultivation because of the potential for stress during stocking procedures. To find optimum transport conditions, several bioassays were performed in the laboratory to evaluate survival of whiteleg shrimp Litopenaeus vannamei 5–30‐day‐old postlarvae under conditions similar to those encountered during transport from the hatchery to nursery and shrimp ponds. Postlarvae were exposed for 4 h to different temperatures and pH levels ammonia concentrations. Survival was significantly reduced after a 4 h exposure to pH 9 and was inversely related to temperature with or without 7 mg L?1 of ammonia. The 15‐ and 20‐day‐old postlarvae had higher survival rates than other ages. The lowest survival occurred in alkali conditions (pH 9), with 7 mg L?1ammonia at 30 and 32°C. To assure optimal survival of postlarvae during transfer from the hatchery to the nursery and shrimp ponds, we recommend temperatures below 28°C, pH no higher than 8, no ammonia and post‐larval age at least 15 days. 相似文献
963.
Santos Y García-Marquez S Pereira PG Pazos F Riaza A Silva R El Morabit A Ubeira FM 《Journal of fish diseases》2005,28(3):165-172
The commercial furunculosis vaccine Aquavac Furovac 5 and an autogenous vaccine, based on the challenge strain, induced immune protection in turbot, Scophthalmus maximus (L.), as shown in challenge tests 120 days post-immunization by injection (relative percentage of survival, RPS = 72-99%). This protective effect lasted for at least 6 months post-immunization at appreciable levels (RPS = 50-52%). Neither the autogenous vaccine nor the commercial vaccine was able to induce significant levels of protection against Aeromonas salmonicida in turbot when administered by immersion. Antibody levels were high or moderate in fish vaccinated by injection with the different vaccines and very low in fish vaccinated by immersion. The field results show that delivering an oral boost after the primary vaccination by injection did not enhance protection of turbot against furunculosis and that water-based (autogenous vaccine) and oil adjuvanted (Alpha Ject 1200) vaccines administered by injection conferred similar levels of protection (RPS > 80%) in turbot. 相似文献
964.
Naití A. Morales Maike Heidemeyer Robert Bauer Sebastian Hernández Enzo Acuña Simon Jan van Gennip Alan M. Friedlander Carlos F. Gaymer 《水产资源保护:海洋与淡水生态系统》2021,31(2):340-355
- Marine protected areas (MPAs) are becoming a widely used tool for the conservation of biodiversity and for fishery management; however, most of these areas are designed without prior knowledge of the basic ecological aspects of the species that they are trying to protect.
- This study investigated the movement of two top predators: the Galapagos shark, Carcharhinus galapagensis, and the yellowtail amberjack, Seriola lalandi, in and around the Motu Motiro Hiva Marine Park (MMHMP) using MiniPAT satellite tags to determine the effectiveness of this MPA for the protection of these species.
- The Galapagos sharks (n = 4) spent most of their tag deployment periods inside the MMHMP. However, high intraspecific variability was observed in their movement dynamics. Daily individual maximum movements ranged from 17 to 58 km and the maximum distance from Salas y Gómez Island, the only emergent island within the MMHMP, ranged from 31 to 139 km.
- The maximum linear distance travelled for a female juvenile Galapagos shark (152 cm total length) was 236 km, which is greater than the maximum distance previously documented for juveniles of this species (<50 km).
- For the yellowtail amberjack (n = 1), 91% of the satellite geolocations were within the MMHMP, with a maximum daily distance travelled of 6 km. The maximum distance travelled between points was 111 km and the maximum distance from Salas y Gómez Island was 62 km.
- All archival tagged fish spent most of their time at depths of <50 m and never left the epipelagic zone. Daytime versus night-time differences were pronounced in all individuals but showed high interindividual variability.
- This study provides a baseline on the movement of these two top predators in the MMHMP and provides valuable insights for the creation of MPAs in the region and elsewhere.
965.
Angela Alegría-Ortega María José Sanín-Pérez Lizette Irene Quan-Young Mario H. Londoño-Mesa 《水产资源保护:海洋与淡水生态系统》2021,31(4):764-776
- The protection of ecosystems with high diversity, such as coral reefs, is not an approach that guarantees their conservation. Thus, maintaining connectivity among coral populations over the long term is a strategy that should be adopted in order to protect diversity and ecological processes. Although coral reefs in Colombia are highly diverse, the population genetics baseline data of keystone species such as Orbicella faveolata are limited.
- To provide current information about the connectivity between populations of O. faveolata in the Colombian Caribbean, the genetic diversity and distribution of O. faveolata was evaluated with analysis of molecular variance (AMOVA), principal components, and migration estimations using 113 genets distributed in six populations. Also, a genetic structure analysis that included the available data for the Caribbean population was conducted, seeking to understand how the Colombian populations relate to the broader region.
- According to the global fixation index (FST) for the Colombian Caribbean, there was no genetic structure (FST = 0.002). Discriminant analysis of principal components (DAPC) showed that Corales del Rosario Archipelago (CR), Este de Isla Fuerte (EIF), San Bernardo Archipelago (SB), and Varadero Reef (VR) were grouped with Oeste de Isla Fuerte (OIF), with Bushnell (BS) representing the most divergent cluster. Migration analysis showed relatively high migration from VR to CR and SB, highlighting the importance of VR as a genetic reservoir for the region. Structure analysis showed that the Colombian population presented a specific genetic identity (FST = 0.254), suggesting that the Colombian Caribbean population could be a peripheral population that contributes significantly to genetic variation and is connected through a complex connectivity process.
- In conclusion, the estimated genetic connectivity reflects the influence of sea surface dynamics over the interpopulation exchange dynamics and the role of protected and nonprotected coral reef in the Colombian Caribbean. Thus, the Colombian Caribbean population of O. faveolata could be relevant to the conservation of genetic diversity on a larger scale.
966.
Ana Eguiguren Enrico Pirotta Kristina Boerder Maurício Cantor Godfrey Merlen Hal Whitehead 《水产资源保护:海洋与淡水生态系统》2021,31(6):1466-1481
- Sperm whales have occupied the waters off the Galápagos Islands, Ecuador, for at least the past 200 years. During the 19th century, they were the target of intensive whaling that severely depleted the population. In recent times, after commercial whaling ended, sperm whales in the region remain vulnerable to multiple threats, especially potential entanglement in fishing gear, which may hinder their ability to recover from the whaling era.
- As a highly mobile, long-lived species, long-term analysis of the habitat use of sperm whales is necessary to establish effective conservation and management strategies. Here, contemporary (1985–2014) and historical (1830–1850) sperm whale habitat use off the Galápagos Islands was analysed and contrasted to the extent of the Galápagos Marine Reserve (GMR). Contemporary habitat use and its variability over time were modelled as a function of geographic, oceanographic, and topographic variables using generalized additive models.
- The fine-scale habitat (<50 km) used by sperm whales was associated with topographic (i.e. depth and slope) and oceanographic characteristics (i.e. relative sea surface temperature and standard deviation of sea surface temperature), but these preferences varied over time.
- While historical and contemporary data indicate that sperm whale habitat primarily occurred within the boundaries of the GMR, in recent years, whales were found up to 30.1% of the time outside the GMR, potentially overlapping with commercial fisheries operating in the area.
- The dynamic nature of the relationship of this nomadic species with its habitat highlights the need of large-scale conservation efforts across the Eastern Tropical Pacific region, including the wide-scale enforcement of regulations requiring the use of Automatic Identification System in fishing vessels, the promotion of on-board fisheries observer programmes, the development of adaptive management strategies, and international collaboration to identify and mitigate threats.
967.
Boris Marioni José António L. Barão-Nóbrega Robinson Botero-Arias Fábio Muniz Zilca Campos Ronis Da Silveira William E. Magnusson Francisco Villamarín 《水产资源保护:海洋与淡水生态系统》2021,31(12):1056-1067
- Crocodilians represent one of the oldest extant vertebrate lineages. They have co-existed with humans throughout the Amazon basin for thousands of years, often having a strong cultural and economic influence on people's lives. Shifts in the socio-economic and political reality of the Amazon basin during the last century have led crocodilian populations to face large variations in their numbers according to different levels of exploitation and strategies for their conservation.
- This article reviews the scientific knowledge obtained between 1945 and 2019 on the biology, conservation and management for the four Amazonian crocodilian (caiman) species (Caiman crocodilus, Melanosuchus niger, Paleosuchus palpebrosus and Paleosuchus trigonatus). It provides a general overview on past and current population status and research efforts involving caimans in the Amazon basin and discusses perspectives for the future.
- The most significant studies on the ecology, genetics and management strategies are examined in more detail and this information is contextualized to provide an overview of the most relevant findings that might explain caiman population trends over the last 75 years.
- Systems for sustainable management in the Amazon basin have been discussed for the past 20 years, but remain rarely applicable. It is necessary to develop new ways to maintain healthy caiman populations through innovative management programmes. Sustainable harvesting of wildlife has been shown to promote conservation targets, especially those initiatives based on community co-management. In this article, we propose some general guidelines for future management schemes, in the expectation that the information provided by the scientific community will be considered fully without political agendas determining the priorities.
968.
Marisa Naia Virgilio Hermoso Sílvia B. Carvalho José Carlos Brito 《水产资源保护:海洋与淡水生态系统》2021,31(7):1886-1900
- Systematic conservation planning in freshwater ecosystems faces multiple challenges because of the dynamic nature of rivers and their multiple dimensions of connectivity. In intermittent hydrological systems connectivity is functional when water is available, allowing the exchange of aquatic individuals between isolated freshwater ecosystems. Integrating these isolated systems in their hydrological context is essential when identifying priority areas for conservation, in order to try to minimize the propagation of threats into target water bodies (management units) from the surrounding landscape.
- Here, the use of a systematic planning approach is demonstrated to identify a set of priority management units to preserve freshwater biodiversity in an arid system of fragmented water bodies immersed in a landscape subject to a range of impacts.
- Twenty-six water-dependent taxa from 59 mountain rock pools (gueltas) of three southern Mauritanian mountains were used as a case study. A conservation planning tool (marxan ) was used to find priority conservation areas to integrate intermittent hydrological systems in their hydrological context, promote connectivity, and minimize the downstream propagation of threats. Three types of connectivity were analysed: (i) no connectivity, (ii) connectivity between gueltas, and (iii) connectivity between gueltas and sub-catchments.
- Considering different types of longitudinal connectivity affects the number and spatial allocation of the priority gueltas selected, and the conservation status of the gueltas and their upstream areas. Incorporating connections between gueltas and upstream locations in the modelling resulted in the selection of gueltas in areas with a low human footprint and in the increased connectivity of the solutions.
- The results obtained revealed important locations for local biodiversity conservation, and the method presented can be used when assessing the propagation of potential waterborne threats into isolated management units. The framework developed allows connectivity to be addressed in conservation planning. It can be replicated in regions with similar isolated habitats that connect through intermittent hydrological systems and can also be applied to lateral and vertical hydrological connectivity.
969.
Theodore W. Hermann Fabrice Duponchelle Leandro Castello Karin E. Limburg Luciana A. Pereira Marília Hauser 《水产资源保护:海洋与淡水生态系统》2021,31(12):1206-1220
- Freshwater environments host roughly half of the world’s fish diversity, much of which is concentrated in large, tropical river systems such as the Amazon. Fishes are critical to ecosystem functioning in the Amazon River basin but face increasing human threats. The basic biology of these species, and particularly migratory behaviour, remains poorly studied, in part owing to the difficulty associated with conducting tagging studies in remote tropical regions.
- Otolith microchemistry can circumvent logistical issues and is an increasingly important tool for studying fish life histories. However, this approach is still new in the Amazon, and its potential and limitations to inform fish conservation strategies remain unclear.
- Here, otolith microchemistry studies in the Amazon are reviewed, highlighting current possibilities, and several key factors that limit its use as a conservation tool in the Amazon are discussed. These include the dearth of spatiotemporal elemental data, poor understanding of environment–fish–otolith pathways, and insufficient funding, facilities, and equipment.
- A research initiative is proposed to harness the potential of this technique to support conservation in the Amazon. Key aspects of the proposal include recommendations for internal and external funding, which are critical to acquiring and maintaining technical staff, cutting-edge equipment, and facilities, as well as fostering regular scientific meetings and working groups. Meetings can facilitate a systematic approach to investigating environment–otolith pathways, broadening the chemical baseline for most Amazonian tributaries, and exploring potential valuable elements.
- These outcomes are urgently needed to conserve biodiversity and ecosystem functioning in the Amazon, especially given threats such as widespread hydroelectric damming. The initiative proposed here could make otolith microchemistry an important, cost-effective tool to inform and foster conservation in the Amazon, and act as a template for other imperilled tropical river basins, such as the Mekong and the Congo.
970.
Dan Laffoley John M. Baxter Diva J. Amon Duncan E.J. Currie Craig A. Downs Jason M. Hall‐Spencer Harriet Harden‐Davies Richard Page Chris P. Reid Callum M. Roberts Alex Rogers Thorsten Thiele Charles R.C. Sheppard Rashid U. Sumaila Lucy C. Woodall 《水产资源保护:海洋与淡水生态系统》2020,30(1):194-208
- The ocean crisis is urgent and central to human wellbeing and life on Earth; past and current activities are damaging the planet's main life support system for future generations. We are witnessing an increase in ocean heat, disturbance, acidification, bio‐invasions and nutrients, and reducing oxygen levels. Several of these act like ratchets: once detrimental or negative changes have occurred, they may lock in place and may not be reversible, especially at gross ecological and ocean process scales.
- Each change may represent a loss to humanity of resources, ecosystem function, oxygen production and species. The longer we pursue unsuitable actions, the more we close the path to recovery and better ocean health and greater benefits for humanity in the future.
- We stand at a critical juncture and have identified eight priority issues that need to be addressed in unison to help avert a potential ecological disaster in the global ocean. They form a purposely ambitious agenda for global governance and are aimed at informing decision‐makers at a high level. They should also be of interest to the general public.
- Of all the themes, the highest priority is to rigorously address global warming and limit surface temperature rise to 1.5°C by 2100, as warming is the pre‐eminent factor driving change in the ocean. The other themes are establishing a robust and comprehensive High Seas Treaty, enforcing existing standards for Marine Protected Areas and expanding their coverage, especially in terms of high levels of protection, adopting a precautionary pause on deep‐sea mining, ending overfishing and destructive fishing practices, radically reducing marine pollution, putting in place a financing mechanism for ocean management and protection, and lastly, scaling up science/data gathering and facilitating data sharing.
- By implementing all eight measures in unison, as a coordinated strategy, we can build resilience to climate change, help sustain fisheries productivity, particularly for low‐income countries dependent on fisheries, protect coasts (e.g. via soft‐engineering/habitat‐based approaches), promote mitigation (e.g. carbon storage) and enable improved adaptation to rapid global change.