Additional thoughts on ovariectomy versus ovariohysterectomy     

McAnulty JF  Bjorling DE  Hardie R  Colopy S 《Journal of the American Veterinary Medical Association》2012,240(8):936-937

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Recombinant Iss as a potential vaccine for avian colibacillosis     

Lynne AM  Kariyawasam S  Wannemuehler Y  Johnson TJ  Johnson SJ  Sinha AS  Lynne DK  Moon HW  Jordan DM  Logue CM  Foley SL  Nolan LK 《Avian diseases》2012,56(1):192-199

Avian pathogenic Escherichia coli (APEC) cause colibacillosis, a disease which is responsible for significant losses in poultry. Control of colibacillosis is problematic due to the restricted availability of relevant antimicrobial agents and to the frequent failure of vaccines to protect against the diverse range of APEC serogroups causing disease in birds. Previously, we reported that the increased serum survival gene (iss) is strongly associated with APEC strains, but not with fecal commensal E. coli in birds, making iss and the outer membrane protein it encodes (Iss) candidate targets for colibacillosis control procedures. Preliminary studies in birds showed that their immunization with Iss fusion proteins protected against challenge with two of the more-commonly occurring APEC serogroups (O2 and O78). Here, the potential of an Iss-based vaccine was further examined by assessing its effectiveness against an additional and widely occurring APEC serogroup (O1) and its ability to evoke both a serum and mucosal antibody response in immunized birds. In addition, tissues of selected birds were subjected to histopathologic examination in an effort to better characterize the protective response afforded by immunization with this vaccine. Iss fusion proteins were administered intramuscularly to four groups of 2-wk-old broiler chickens. At 2 wk postimmunization, chickens were challenged with APEC strains of the O1, O2, or O78 serogroups. One week after challenge, chickens were euthanatized, necropsied, any lesions consistent with colibacillosis were scored, and tissues from these birds were taken aseptically. Sera were collected pre-immunization, postimmunization, and post-challenge, and antibody titers to Iss were determined by enzyme-linked immunosorbent assay (ELISA). Also, air sac washings were collected to determine the mucosal antibody response to Iss by ELISA. During the observation period following challenge, 3/12 nonimmunized chickens, 1/12 chickens immunized with 10 microg of GST-Iss, and 1/12 chickens immunized with 50 microg of GST-Iss died when challenged with the O78 strain. No other deaths occurred. Immunized chickens produced a serum and mucosal antibody response to Iss and had significantly lower lesion scores than nonimmunized chickens following challenge, regardless of the challenge strain. This study expands on our previous report of the value of Iss as an immunoprotective antigen and demonstrates that immunization with Iss can provide significant protection of chickens against challenge with three different E. coli strains.  相似文献   

Latent class analysis of the diagnostic characteristics of PCR and conventional bacteriological culture in diagnosing intramammary infections caused by Staphylococcus aureus in dairy cows at dry off     

Sara Ellinor Cederl?f  Nils Toft  Bent Aalbaek  Ilka Christine Klaas 《Acta veterinaria Scandinavica》2012,54(1):65

Background

Staphylococcus aureus is one of the most common causes of intramammary infections in dairy cows at dry off. Reliable identification is important for disease management on herd level and for antimicrobial treatment of infected animals. Our objective was to evaluate the test characteristics of PathoProof ™ Mastitis PCR Assay and bacteriological culture (BC) in diagnosing bovine intramammary infections caused by S. aureus at dry off at different PCR cycle threshold (Ct)-value cut-offs.

Methods

Sterile quarter samples and non-sterile composite samples from 140 animals in seven herds were collected in connection with the dairy herd improvement (DHI) milk recording. All quarter samples were analyzed using BC whereas all composite samples were analyzed with PathoProof ™ Mastitis PCR Assay. Latent class analysis was used to estimate test properties for PCR and BC in the absence of a perfect reference test. The population was divided into two geographically divided subpopulations and the Hui-Walter 2-test 2-populations model applied to estimate Se, Sp for the two tests, and prevalence for the two subpopulations.

Results

The Se for PCR increased with increasing Ct-value cut-off, accompanied by a small decrease in Sp. For BC the Se decreased and Sp increased with increasing Ct-value cut-off. Most optimal test estimates for the real-time PCR assay were at a Ct-value cut-off of 37; 0.93 [95% posterior probability interval (PPI) 0.60-0.99] for Se and 0.95 [95% PPI 0.95-0.99] for Sp. At the same Ct-value cut-off, Se and Sp for BC were 0.83 [95% PPI 0.66-0.99] and 0.97 [95% PPI 0.91-0.99] respectively. Depending on the chosen PCR Ct-value cut-off, the prevalence in the subpopulations varied; the prevalence increased with increasing PCR Ct-value cut-offs.

Conclusion

Neither BC nor real-time PCR is a perfect test in detecting IMI in dairy cows at dry off. The changes in sensitivity and prevalence at different Ct-value cut-offs for both PCR and BC may indicate a change in the underlying disease definition. At low PCR Ct-value cut-offs the underlying disease definition may be a truly/heavily infected cow, whereas at higher PCR Ct-value cut-offs the disease definition may be a S. aureus positive cow.  相似文献   

Effect of sample volume size and sampling method on feline longitudinal myocardial velocity profiles from color tissue Doppler imaging     

Sara Granström  Christian Bressen Pipper  Rasmus Møgelvang  Peter Sogaard  Jakob Lundgren Willesen  Jørgen Koch 《Journal of Veterinary Cardiology》2012,14(4):479-488

ObjectivesThe aims of this study were to compare the effect of sample volume (SV) size settings and sampling method on measurement variability and peak systolic (s′), and early (e′) and late (a′) diastolic longitudinal myocardial velocities using color tissue Doppler imaging (cTDI) in cats.AnimalsTwenty cats with normal echocardiograms and 20 cats with hypertrophic cardiomyopathy.MethodsWe quantified and compared empirical variance and average absolute values of s′, e′ and a′ for three cardiac cycles using eight different SV settings (length 1,2,3 and 5 mm; width 1 and 2 mm) and three methods of sampling (end-diastolic sampling with manual tracking of the SV, end-systolic sampling without tracking, and random-frame sampling without tracking).ResultsNo significant difference in empirical variance could be demonstrated between most of the tested SVs. However, the two settings with a length of 1 mm resulted in a significantly higher variance compared with all settings where the SV length exceeded 2 mm (p < 0.001). There was an overall significant effect of sampling method on the variability of measurements (p = 0.003) and manual tracking obtained the lowest variance. No difference in average values of s′, e′ or a′ could be found between any of the SV settings or sampling methods.ConclusionWithin the tested range of SV settings, an SV length of 1 mm resulted in higher measurement variability compared with an SV length of 3 and 5 mm, and should therefore be avoided. Manual tracking of the sample volume is recommended.  相似文献   

Atypical Radiologic Appearance of a Second Cervical Vertebral Fracture in a Horse     

Anna Ehrle  Sara Jones  Patricia Klose  Christoph Lischer 《Journal of Equine Veterinary Science》2012

This report presents the case of a 21-year-old Warmblood mare referred to the clinic with neurological dysfunctions and considerable signs of discomfort. A fracture of the caudal vertebral body of C2 with a very unusual radiographic appearance was diagnosed, and the mare was euthanized due to deterioration in neurological status. Histology revealed no evidence of neoplasia or pathological fracture.  相似文献   

In vitro evaluation of canine hemostasis following dilution with hydroxyethyl starch (130/0.4) via thromboelastometry     

Sara Falco DVM  Barbara Bruno DVM  Cristiana Maurella DVM  MS  Claudio Bellino DVM  PhD  Antonio D'Angelo DVM  PhD  DECVN  DECBHM  Paola Gianella DVM  PhD  DACVIM  Alberto Tarducci DVM  Renato Zanatta DVM  PhD  Antonio Borrelli DVM  PhD  MS 《Journal of Veterinary Emergency and Critical Care》2012,22(6):640-645

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BODIUM—A systemic approach to model the dynamics of soil functions     

Sara König  Ulrich Weller  Bibiana Betancur-Corredor  Birgit Lang  Thomas Reitz  Martin Wiesmeier  Ute Wollschläger  Hans-Jörg Vogel 《European Journal of Soil Science》2023,74(5):e13411

The increasing demand for biomass for food, animal feed, fibre and bioenergy requires optimization of soil productivity, while at the same time, protecting other soil functions such as nutrient cycling and buffering, carbon storage, habitat for biological activity and water filter and storage. Therefore, one of the main challenges for sustainable agriculture is to produce high yields while maintaining all the other soil functions. Mechanistic simulation models are an essential tool to fully understand and predict the complex interactions between physical, biological and chemical processes of soils that generate those functions. We developed a soil model to simulate the impact of various agricultural management options and climate change on soil functions by integrating the relevant processes mechanistically and in a systemic way. As a special feature, we include the dynamics of soil structure induced by tillage and biological activity, which is especially relevant in arable soils. The model operates on a 1D soil profile consisting of a number of discrete layers with dynamic thickness. We demonstrate the model performance by simulating crop growth, root growth, nutrient and water uptake, nitrogen cycling, soil organic matter turnover, microbial activity, water distribution and soil structure dynamics in a long-term field experiment including different crops and different types and levels of fertilization. The model is able to capture essential features that are measured regularly including crop yield, soil organic carbon, and soil nitrogen. In this way, the plausibility of the implemented processes and their interactions is confirmed. Furthermore, we present the results of explorative simulations comparing scenarios with and without tillage events to analyse the effect of soil structure on soil functions. Since the model is process-based, we are confident that the model can also be used to predict quantities that have not been measured or to estimate the effect of management measures and climate states not yet been observed. The model thus has the potential to predict the site-specific impact of management decisions on soil functions, which is of great importance for the development of a sustainable agriculture that is currently also on the agenda of the ‘Green Deal’ at the European level.  相似文献   

Exertional Heat-Related Illnesses at the Grand Canyon National Park, 2004–2009     

CDR Rebecca S. Noe  Ekta Choudhary  LCDR Jennifer Cheng-Dobson  Amy F. Wolkin  CDR Sara B. Newman 《The Forestry Chronicle》2013,24(4):422-428

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Diagnostic Accuracy of Tissue Impedance Measurement Interpretation for Correct Veress Needle Placement in Feline Cadavers     

Sara Hyink DVM  Jacqueline C. Whittemore DVM  PhD  Diplomate ACVIM  Amanda Mitchell DVM  Ann Reed MS 《Veterinary surgery : VS》2013,42(5):623-628

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Innovative Approach to Laparoscopic Adrenalectomy for Treatment of Unilateral Adrenal Gland Tumors in Dogs     

Elaine C. Naan DVM  Diplomate ECVS  Jolle Kirpensteijn DVM  PhD  Diplomate ECVS  ACVS  Gilles P. Dupré DVM  Diplomate ECVS  Sara Galac DVM  PhD  MaryAnn G. Radlinsky DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2013,42(6):710-715

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