We studied the importance of effective rainfall for interannual variation in water use efficiency (WUE) and tree-ring growth
of Chinese pine (Pinus tabulaeformis Carr.) and black locust (Robinia pseudoacacia L.) by examining correlations of seasonal precipitation with annual values of stable carbon isotope ratio (δ13C) and tree-ring width in early and late wood. The correlations with precipitation were examined for each month and for periods
of all possible lengths from 2 to 22 months starting from January of the previous year to October of the current year. The
period with the highest correlation was adopted as the most effective rainfall season for interannual variations in WUE and
tree-ring width. In early wood, precipitation during the dry season (October to May) before the growing season was negatively
correlated with δ13C in pine trees and positively correlated with ring width in pine and locust trees. In late wood, rainfall during the growing
season in the current year was negatively correlated with δ13C in pine and locust trees, and positively correlated with ring width in locust trees. Our results demonstrated the differences
in the water use strategies of pine and locust trees. The δ13C in pines indicated higher WUE and more conservative water use than in locust trees. Precipitation during the dry season
affected the interannual variation in WUE and tree-ring growth in pine and locust trees, indicating that rainfall during the
dry season is important for carbon gain and tree-ring growth during the following growing season. 相似文献
A substance produced extracellularly by a marine bacterium, Pseudomonas C55a-2 strain, and possessing algal-killing activity against a diatom, Chaetocerosceratosporum, was isolated from the culture supernatant of the bacterium in order to identify its chemical structure. The algicidal substance
extracted with ethyl acetate from the culture supernatant was purified by using Sep-Pak treatment, reverse phase column chromatography
with Sephadex LH-20, and high performance liquid chromatography (HPLC) with Mightysil RP-18 GP. The purified substance was
identified as 2,3-indolinedione (isatin) (molecular weight 147) based on hydrogen-nuclear magnetic resonance (H-NMR) and gas
chromatograph-mass spectrometry (GC-MS) analysis. Among artificial synthetic compounds examined for algicidal activity against
Chaetoceros cells on the double-layer plates, 2,3-indolinedione (isatin) appeared the most effective and indoline showed relatively less
activity than isatin. 相似文献
The significance of Mn and Fe for the growth of a coastal marine diatom Thalassiosira weissflogii was investigated by performing culture experiments containing macronutrients with either Mn or Fe, or both. Only the addition
of both Mn and Fe induced the highest growth rates and maximal cell yields. Maximal growth was maintained in continuous culture
media, which were repeatedly prepared by an inoculation of pre-culture and the addition of both Mn and Fe to the control culture
medium containing macronutrients. In particular, it was found that the full growth recovery in Mn-sufficient medium (without
added Fe) is accomplished by the addition of Fe even after several days’ incubation. On the contrary, there was no sufficient
growth recovery by the addition of Mn after a long incubation time in Fe-sufficient medium but without additional Mn. These
results suggest that T. weissflogii in Mn-sufficient waters retains the ability for full physiological recovery for a long time, probably resulting from the
decrease in the oxidative stress of phytoplankton by the production of antioxidant enzyme Mn superoxide dismutase during a
long incubation period. 相似文献
In order to elucidate mechanisms of tyramine accumulation during fish sauce production, two tyramine-producing bacterial strains, referred to as TyrA and TyrB, were isolated from fish sauce mash accumulating over 141 mg of tyramine per 100 g of sample. Both strains were identified as Tetragenococcus halophilus based on phenotypic characterization and a 16S rRNA gene sequence analysis. Molecular analysis of the tyramine-producing gene in the two strains confirmed the presence of a ~30-kb plasmid encoding a single copy of the pyridoxal phosphate-dependent tyrosine decarboxylase gene (tdcA) along with three other genes related to tyramine synthesis (tdc cluster). The complete nucleotide sequences of plasmids extracted from the two strains indicated that both plasmids were almost identical, except for a 1.6-kb transposon sequence in the plasmid from the strain TyrB. Both plasmids had a replication region, a plasmid maintenance region, and two putative mobile genetic elements located upstream and downstream of the tdc cluster. This structure was identical to that of tetragenococcal plasmids encoding histidine decarboxylase (hdcA), which were sequenced previously. These results suggest a common origin for plasmids encoding hdcA and tdcA. In addition, the genes for both these biogenic amines are distributed among tetragenococcal species via this plasmid. 相似文献
It was previously reported that (-)-epigallocatechin-3-O-gallate (EGCG) suppresses the expression of the high-affinity IgE receptor FcepsilonRI in human basophilic cells and that this suppressive effect is associated with EGCG binding to the cell surface. This study examined the effects of five methylated derivatives of EGCG, (-)-epigallocatechin-3-O-(3-O-methyl)gallate (EGCG 3' 'Me), (-)-epigallocatechin-3-O-(4-O-methyl)gallate (EGCG 4' 'Me), (-)-4'-O-methyl-epigallocatechin-3-O-gallate (EGCG 4'Me), (-)-epigallocatechin-3-O-(3,4-O-methyl)gallate (EGCG 3' '4' 'diMe), and (-)-4'-O-methyl-epigallocatechin-3-O-(4-O-methyl)gallate (EGCG 4'4' 'diMe) on FcepsilonRI expression and ERK1/2 phosphorylation, and each of their cell surface binding activities was measured. Of these five methylated derivatives, three that are methylated at the 3' '- and/or 4' '-position, EGCG 3' 'Me, EGCG 4' 'Me, and EGCG 3' '4' 'diMe, suppressed FcepsilonRI expression and ERK1/2 phosphorylation, although the suppressive effects were lower than that of EGCG. EGCG 4'Me and EGCG 4'4' 'diMe, both of which are methylated at the 4'-position, did not demonstrate a suppressive effect. Furthermore, it was found that EGCG 3' 'Me, EGCG 4' 'Me, EGCG 3' '4' 'diMe, and EGCG 4'Me, which are methylated at the 3' '- and/or 4' '-positions or the 4'-position, could bind to the cell surface even though their binding activities were lower than that of EGCG. Only EGCG 4'4' 'diMe, which is methylated at both the 4'- and 4' '-positions, could not bind. These results suggest that the trihydroxyl structure of the B ring is essential for EGCG to exert the suppressive effects and that the hydroxyl groups on both the 4'-position in the B ring and the 4' '-position in the gallate are crucial for the cell surface binding activity of EGCG. 相似文献
Unprecedented large-scale algal blooms were observed during autumn 2021 around the Katsurakoi fishing port, Kushiro, eastern Hokkaido, Japan. Monitoring of shoreline water showed that chlorophyll a (Chl a) concentrations and the cell density of Karenia spp., dominated by Karenia selliformis, repeatedly increased synchronously between September and November 2021. These increases were associated with a southerly wind-driven current, which transported offshore water on the shelf towards the shoreline at the sea surface. The blooms were prolonged as a result of algal accumulation in the semi-closed fishing port. The maximum Chl a concentration and cell density exceeded 50 µg Chl a/L and 104 cells/mL, respectively. During the autumn bloom of Karenia spp., the nitrate?+?nitrite and phosphate concentrations in the water were lower than those in 2019 and 2020, and the silicate concentration was comparable. The ammonium concentration during the bloom was notably higher than before the bloom period, reaching 15 µM. Mass mortality of several fish species and echinoderms that were cultured using rearing water intake from the same shoreline occurred synchronously with the increase in Karenia spp.
Tetraploid varieties of lilies have superior agronomic traits such as large flowers and resistance to physiological disorders.
In the present study, we attempted to induce 2n pollen of Asiatic hybrid lilies by arresting the meiotic process with nitrous oxide (N2O) gas. To determine which meiotic stage is optimal for induction of 2n pollen, plants with attached buds at different meiotic stages were treated with N2O for 24 h in a pressure-tolerant cylinder. A few 2n pollen grains were induced using plants with anthers in prophase I, whereas mixed pollen grains of differing size were produced
using plants undergoing meiotic metaphase predominantly in anthers. Although normal lily pollen grains are elliptical, nitrous
oxide exposure induced giant pollen grains that appeared spherical. Flow cytometry analysis showed that the giant pollen grains
were diploid. When mixed pollen that included normal and giant pollen was crossed to tetraploid cultivars, the resulting seedlings
were tetraploid and aneuploid, indicating that the giant pollen grains were diploids that could generate tetraploid seedlings
through fusion to diploid eggs supplied from a tetraploid female parent. Thus, treatment with N2O is useful for the production of 2n lily pollen and may provide a new approach for tetraploid lily breeding. 相似文献
ABSTRACT: Adductor muscles dissected from live scallop Patinopecten yessoensis were stored in oxygenated artificial sea water. The initial muscle adenosine triphosphate (ATP) level, approximately 7.5 µmol/g, remained longer at 5°C than at either 0 or 10°C. The pH of sea water decreased continuously and the consumption of dissolved oxygen increased even after muscle ATP was almost exhausted. The number of viable microbes, measured as colony-forming units (c.f.u.) in the muscle, increased to reach a plateau at approximately 107−108 c.f.u./g, while muscle ATP remained at high levels. After this time, muscle ATP sharply decreased. Antibiotics or sorbate added into the oxygenated sea water effectively inhibited both the growth of microbes and the decrease in the pH of sea water. Under these conditions, the retention period of muscle ATP was greatly extended. Thus, it seems most likely that scallop adductor muscle cells are suffocated by the limitation of oxygen supply caused by aerobic microbes grown on the surface of muscle tissue. 相似文献
The effects of non-ionic (sorbitol, maltose, trehalose) and ionic compounds (Na-glutamate, Na-acetate, Na-sulfate, ammonium
sulfate) on freeze denaturation of myosin subfragment-1 (S-1) and of myofibrils were compared. Sugars, Na-glutamate and Na-acetate
well suppressed the freeze denaturation of myofibrils as well as S-1 in a concentration dependent manner. Although sulfate
suppressed freeze denaturation of S-1 irregularly, it accelerated myofibril denaturation. It was concluded that sulfate salts
were useless as cryoprotectant for myofibrils. Stabilization extent by F-actin in frozen storage was much less than that in
heating. 相似文献