Demonstration of Toxoplasma gondii antigen in stillborn piglets using immunoperoxidase technique     

M Haritani  K Shimura  I Iwabuchi  M Kobayashi  M Narita 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(4):954-956

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Detection of the schizont stage in experimentally infected cattle with Japanese Theileria sergenti     

T Minami  S Kawazu  K Shimura  K Fujisaki  T Kamio  S Ito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(3):601-604

Calves infected with a sporozoite suspension of the Ikeda strain of Theileria sergenti were examined in attempts to detect the schizont stage. A suspension equivalent to the contents of 70-200 ticks was inoculated subcutaneously into 10 splenectomized calves. Schizonts and micromerozoites were found in the prescapular lymph nodes of 5 calves about 8 days after the sporozoite inoculation. Schizonts detected at the time of nuclear division had large and diffuse nuclei, and many of the schizonts were very large and extracellular in lymph node smears. The schizonts were also detected by the direct fluorescent antibody staining.  相似文献   

A double screening trial for the development of drugs against Theileria sergenti in cattle using Babesia rodhaini and Plasmodium berghei in mice     

S Ito  K Shimura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(1):101-109

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Phenotyping of lymphocyte subsets in the vascular and epithelial lesions of a cow with malignant catarrhal fever.     

Y Nakajima  E Momotani  Y Ishikawa  T Murakami  N Shimura  M Onuma 《Veterinary immunology and immunopathology》1992,33(3):279-284

Surface marker analysis of lymphoid cells infiltrating the vascular and epithelial lesions of a cow with malignant catarrhal fever (MCF) was conducted by immunohistochemistry using ten monoclonal antibodies. The majority of lymphoid cells in these lesions had BoCD8, BoCD6 or BoCD2, but they rarely possessed N-cell (BoCD5+/BoCD4-/BoCD8-, non-T non-B) markers. Similar reactivity was seen in lymphoid cells of perivascular infiltrates in the liver, heart and brain, and in T-dependent areas of lymph nodes. These results suggest involvement of cytotoxic T-lymphocytes in the pathogenesis of MCF.  相似文献   

A stochastic-modeling evaluation of the foot-and-mouth-disease survey conducted after the outbreak in Miyazaki, Japan in 2000     

Tsutsui T  Minami N  Koiwai M  Hamaoka T  Yamane I  Shimura K 《Preventive veterinary medicine》2003,61(1):45-58

When foot-and-mouth-disease (FMD) was identified in Miyazaki prefecture in March 2000, Japan conducted an intensive serological and clinical survey in the areas surrounding the index herd. As a result of the survey during the 21 days of the movement-restriction period, two infected herds were detected and destroyed; there were no other cases in the months that followed. To evaluate the survey used for screening the disease-control area and surveillance area, we estimated the herd-level sensitivity of the survey (HSe) through a spreadsheet model using Monte-Carlo methods. The Reed-Frost model was incorporated to simulate the spread of FMD within an infected herd. In the simulations, 4, 8 and 12 effective-contact scenarios during the 5-day period were examined. The estimated HSes of serological tests (HSeE) were 71.0, 75.3 and 76.3% under the 4, 8 and 12 contact scenarios, respectively. The sensitivity analysis showed that increasing the number of contacts beyond 12 did not improve HSeE, but increasing the number of sampled animals and delaying the dates of sampling did raise HSeEs. Small herd size in the outbreak area (>80% of herds have <20 animals) seems to have helped in maintaining HSeE relatively high, although the serological inspection was carried out before sero-positive animals had a chance to increase in infected herds. The estimated herd-level specificity of serological tests (HSpE) was 98.6%. This HSpE predicted 224 false-positive herds (5th percentile estimate was 200 and 95th percentile was 249), which proved close to the 232 false-positive herds actually observed. The combined-test herd-level sensitivity (serological and clinical inspections combined; CTHSe), averaged 85.5, 87.6 and 88.1% for the 4, 8 and 12 contact scenarios, respectively. Using these CTHSes, the calculated probability that no infected herd was overlooked by the survey was > or =62.5% under the most-conservative, four-contact scenario. The probability that no more than one infected herd was overlooked was > or =89.7%.  相似文献   

Theileria sergenti: a simple method for isolation of piroplasms from erythrocytes     

K Fujisaki  T Kamio  Y Nakamura  K Shimura  Y Takahashi  S Kawazu  S Shimizu  T Minami  S Ito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(2):457-459

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Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva     

Graham SP  Saya R  Awino E  Ngugi D  Nyanjui JK  Hecker R  Taracha EL  Nene V 《Veterinary immunology and immunopathology》2007,115(3-4):383-389

Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymorphic immunodominant molecule from Theileria parva emulsified with immunostimulatory CpG oligodeoxynucleotides in adjuvant had no effect on the induction of antibody responses including the isotype profile, but significantly enhanced the induction of cytolytic responses that were mediated by CD4+CD3+ T cells utilizing the perforin-granzyme pathway.  相似文献   

Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila   总被引：39，自引：0，他引：39  

K Hoshijima  K Inoue  I Higuchi  H Sakamoto  Y Shimura 《Science (New York, N.Y.)》1991,252(5007):833-836

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Electrolyte,acid–base,and hemoglobin oxygen affinity alterations following irradiation and storage of canine packed red blood cells          下载免费PDF全文

Saya A. Press  Edward S. Cooper  Jason W. Stull 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(4):580-588

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