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961.
Coding channels in the taste system of the rat 总被引:1,自引:0,他引:1
Basic taste qualities are thought to be perceived independently, yet discrete neural coding channels have not been demonstrated in the central nervous system. The response profiles of taste cells in the nucleus tractus solitarius (NTS) of the rat were categorized into four groups, and the effects of amiloride, a passive sodium channel blocker, on each were determined. NTS neurons that responded specifically to sodium chloride (NaCl) or to NaCl and sugars were suppressed by amiloride; those broadly sensitive to salts, acids, and bitter stimuli were unaffected. Moreover, the response profile evoked by NaCl lost its distinctiveness after treatment with amiloride, becoming similar to those evoked by acids and quinine. Receptors that respond to sodium must relay their information through independent coding channels to identifiable subgroups of NTS neurons, the activity of which is responsible for the perception of saltiness. 相似文献
962.
Corn seedlings release large amounts of terpenoid volatiles after they have been fed upon by caterpillars. Artificially damaged seedlings do not release these volatiles in significant amounts unless oral secretions from the caterpillars are applied to the damaged sites. Undamaged leaves, whether or not they are treated with oral secretions, do not release detectable amounts of the terpenoids. Females of the parasitic wasp Cotesia marginiventris (Cresson) learn to take advantage of those plant-produced volatiles to locate hosts when exposed to these volatiles in association with hosts or host by-products. The terpenoids may be produced in defense against herbivores but may also serve a secondary function in attracting the natural enemies of these herbivores. 相似文献
963.
T.L. Blanchard DVM MS Dip ACT J.B. Jorgensen BS D.D. Varner DVM MS Dip ACT D.W. Forrest MS PhD J.W. Evans MS PhD 《Journal of Equine Veterinary Science》1996,16(5):195-201
To investigate effects of thermally-induced testicular degeneration on hormonal and seminal parameters in stallions, the scrotum was insulated for 36 hours in two mature (5-year-old mixed breed and 11-year-old Throughbred) stallions. Semen was collected daily for 10 days (DSO) prior to, and at intervals after, scrotal insulation. When DSO determinations were not being made, semen was collected 3 times weekly. Jugular blood samples were collected at 15-minute intervals for 6 hours from each stallion prior to, and at intervals after, scrotal insulation. A mouse interstitial cell testosterone assay was modified to quantify biologic activity of equine luteinizing hormone (BLH) in plasma samples. Immunoactive luteinizing hormone (ILH) and testosterone (T) concentrations were determined in plasma samples by routine RIA procedures. Percentages of progressively motile and morphologically normal spermatozoa began to decrease by 1 to 2 weeks postinsulation, reached nadir values at 3 to 3-1/2 weeks postinsulation, and returned to preinsulation values by 7 weeks postinsulation. Total number of spermatozoa and total number of progressively motile, morphologically normal spermatozoa in ejaculates at DSO returned to normal by 8 weeks postinsulation in stallion 2 and 12 weeks postinsulation in stallion 1. Concentrations of BLH and ILH increased, and while T concentrations decreased, immediately postinsulation. The increase in ILH concentrations was greater than the increase in BLH concentrations, resulting in a decrease in the BLH:ILH (B:I) ratio. Following the peak in LH secretion immediately postinsulation, LH concentrations gradually decreased while T concentrations increased. The B:I ratio was elevated from 1 to 13 weeks postinsulation compared to immediately postinsulation. In addition to changes in spermatozoal quality in ejaculates, stallion response to scrotal insulation included increased secretion of luteinizing hormone and impaired Leydig cell function (as determined by reduced testosterone concentration in circulating plasma). The proportion of biologically active LH secreted in response to thermal testicular injury increased during the recovery phase. 相似文献
964.
P.G. Gibbs PhD G.D. Potter PhD G.T. Schelling PhD J.L. Kreider PhD C.L. Boyd DVM 《Journal of Equine Veterinary Science》1996,16(2):60-65
Mature ponies fitted with permanent ileal cannulas were used in two 3×3 Latin square experiments to quantify prececal, postileal and total tract digestion of N. In trial 1, corn, oats and sorghum were each fed with coastal Bermuda grass hay in 75:25 ratios. Apparent prececal digestibilities were similar (P>.05) and averaged 46.6%. By-difference procedures were employed to calculate digestibility of the cereal grain N only and apparent prececal N digestibility averaged 57.1%. In trial 2, a basal corn and hay diet was supplemented with cottonseed meal and soybean meal. Apparent total tract N digestibilities were similar (P>.05) across treatments, and prececal digestibility averaged 45.6%. By-difference calculations were used to determine digestibility of SBM and CSM N alone. Apparent prececal digestibility of SBM was 52.5% and was lower (P<.05) than 81.2% for CSM. It is possible that inadequate or excessive heat treatment of SBM affected enzymatic digestion. True digestibility of total rations fed in trial 2 was estimated by linear regression of nitrogen digested on nitrogen intake or N presented to the large intestine. True N digestibility of diets containing SBM and CSM was 54.7% and 69.4%, respectively. 相似文献
965.
Portfolio theory, an established technique in the selection of stocks and financial instruments for investment purposes, was used as a means of selecting an economic mix of veterinary interventions in a dairy herd. The expected return on investment in six veterinary services and the associated standard deviations (as measures of risk) were calculated using computer simulation modeling techniques. Mathematical programming techniques were applied to identify the optimal combinations of veterinary interventions which maximize expected financial returns while minimizing risk. Parametric analysis was used to show how risk (standard deviation) increases as the required expected return is increased along the efficient risk-return frontier of these optimal combinations. This exercise emphasizes the importance of regarding risk in addition to expected return when considering investment in veterinary services. 相似文献
966.
Concentrations of bioactive and immunoactive luteinizing hormone in bovine anterior pituitary tissue
G E Moss R P Lemenager J R Parfet B M Adams T E Adams 《Domestic animal endocrinology》1988,5(2):185-190
Tissue concentrations of LH as determined by radioimmunoassay (RIA) may not accurately measure actual changes that could occur in biological activity of the hormone. To examine this possibility, pituitary homogenates from 135 beef cows in various physiological states were analyzed for content of LH by both RIA and an in vitro bioassay. The ratio of biological/immunological active concentrations of LH remained constant (.52 +/- .02) even though tissue concentrations of immunoactive LH differed among groups. Tissue concentrations of bioactive LH were linearly related to, and highly correlated with (P less than .001), tissue concentrations of immunoactive LH. These data indicate that only a fraction of the immunoactive LH in the bovine pituitary is biologically active. However, this fraction does not vary with the reproductive status or plane of nutrition. 相似文献
967.
Interaction of sub-epithelial connective tissue components with Staphylococcus aureus and coagulase-negative staphylococci from bovine mastitis 总被引:4,自引:0,他引:4
Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from bovine mastitis were examined for their ability to interact with 125I-labelled fibronectin, fibrinogen and type II collagen. Their relative surface hydrophobicity and production of extracellular capsule were also investigated. Almost all S. aureus strains bound fibronectin (mean value 23%), fibrinogen (mean value 12%) and type II collagen (mean value 16%). CNS bound fibronectin (mean value 6%) and type II collagen (mean value 7%), but not fibrinogen (mean value 2%). The specificity of binding of these proteins to S. aureus strain F1440 and to coagulase-negative Staphylococcus chromogenes strain BO52 was studied by adding an excess of unlabelled proteins. Fibronectin and collagen binding were observed to be specific, varying between 50 and 75%, whereas the specificity of fibrinogen binding to S. aureus strain F1440 was lower (26%). Most of the S. aureus strains (63%) showed very high surface hydrophobicity (autoaggregation) or lower hydrophobicity (29% of the strains) and the rest were hydrophilic. None of the CNS strains autoaggregated, 44% were classified as hydrophilic strains. Hydrophilic strains (except the reference strains) did not show extracellular capsule production. However, the encapsulated (reference) strains showed low binding to these proteins as compared to their unencapsulated variants. Pre-treatment of S. aureus strain F1440 and S. chromogenes strain BO52 with trypsin decreased their fibronectin binding capacity and surface hydrophobicity, whereas pre-treatment with bovine milk (except on collagen binding to strain F1440) did not significantly affect binding to these proteins. These data indicate that S. aureus and CNS isolated from bovine udder infection have the ability to bind to tissue matrix and plasma proteins which may be exposed in the traumatized or toxin-damaged udder epithelial lesions. 相似文献
968.
The effects of age, environmental temperature and relative humidity on the bacterial flora of the nose and trachea of calves were investigated by sequential sampling of three groups each of eight Friesian-Holstein male calves kept in three different environmental conditions. All calves were vaccinated with a live attenuated vaccine against infectious bovine rhinotracheitis (IBR) when they were 12 weeks old. Nasal and tracheal swabs were collected at 14-day intervals for bacteriological examinations. The upper respiratory tract of calves started to be colonized by various species of bacteria within the first day of life. Although they were born at different periods of the year, the calves in all three groups had similar bacterial loads in their noses and tracheas when they were 1 day old (P greater than 0.05). The total bacterial colony forming units (BCFU) were highly variable from calf to calf and from one time of sampling to another. Despite these variations, there were age-related increases in the total BCFU in nasal and tracheal swabs in all experiments. These increases were influenced by environmental temperature. Vaccination of the calves with a live IBR vaccine appeared to enhance the bacterial colonization of the upper respiratory tract. 相似文献
969.
P R Scott 《The British veterinary journal》1992,148(1):15-22
Analysis of cerebrospinal fluid (CSF) samples from normal sheep and from cases of some common neurological diseases revealed a significant increase (P less than 0.05) in the group mean CSF protein concentration for meningitis, listeriosis and spinal abscess but not for scrapie, spinal injury, ovine pregnancy toxaemia or polioencephalomalacia. The CSF white blood cell count (WBC) was significantly increased (P less than 0.05) in the meningitis group and in those cases of listeriosis which failed to respond to antibiotic therapy. All cases of bacterial infection of the central nervous system (CNS) could be identified by the combined interpretation of the protein concentration and the differential WBC count. It is concluded that CSF analysis is useful clinically in differentiating traumatic from infective spinal lesions and toxic or metabolic lesions from bacterial meningitis in sheep. 相似文献
970.
A geometrical model of weft knitted spacer structures made with mono-filament yarn has been analysed to understand the spacer
yarn path. Theoretical models have been created to predict the porosity and the radius of the capillaries of a knitted spacer
structure depending on their geometrical parameters, such as course spacing, wale spacing, stitch length, fabric thickness,
count of yarn and fibre density. Polyester knitted spacer fabrics were produced with different parameters; their porosity
was determined by measuring the weight and compared with the theoretical porosity. The validity of the model was confirmed
by experimental results. The porosity of knitted spacer structures made out of mono-filament yarn can be maintained above
a certain level by adjusting the fabric parameters such as fabric thickness, course spacing and wale spacing. 相似文献