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Pardon KH Graney SD Capone DL Swiegers JH Sefton MA Elsey GM 《Journal of agricultural and food chemistry》2008,56(10):3758-3763
The individual diastereoisomers of the cysteine conjugate of 3-mercaptohexanol (4) were synthesized with high isomeric purity (>98%). On treatment with Apotryptophanase enzyme, the 3R diastereoisomer of 4 gave an 82% yield of the R enantiomer of 1, with no trace of the 3S enantiomer present. Conversely, the 3S diastereoisomer of 4 gave the 3S enantiomer of 1 (43%) accompanied by a trace of the 3R form (S/R = 98.5:1.5), reflecting the diastereomeric purity of the cysteine conjugate. The same stereochemical outcome was observed when the individual diastereoisomers of 4 were added to fermentations with the Saccharomyces cerevisiae AWRI 1655 yeast strain, which gave 1 in 1% yield. A d(10)-analogue of 1 was synthesized and used as an internal standard to determine, by gas chromatography-mass spectrometry (GC-MS), the amounts of 1 formed in these transformations. 相似文献
94.
Sara Sánchez-Moreno Sean Smukler Howard Ferris Anthony T. O’Geen Louise E. Jackson 《Biology and Fertility of Soils》2008,44(5):727-744
The aim of this paper was to assess biodiversity among different habitats of an organic farm and the relationships between
some soil properties, nematode taxonomic diversity, and soil food web condition. Eight habitats were studied in the farm:
ponds, ditches, a riparian corridor, hedgerows, and four agricultural fields (mustard, oats, fallow, and legumes). The undisturbed
riparian corridor had higher soil and concentrations, and potentially mineralizable N and higher abundances of bacterivore nematodes and longer food webs. Canonical
correlation analysis showed associations between habitats and nematode trophic groups: predatory and bacterial-feeding nematodes
in the riparian corridor and hedgerows, omnivore nematodes in the ponds and ditches, and fungal-feeding nematodes in the legume
field. Soil chemical and physical properties mirrored the aboveground farm patterns and were more similar among habitats that
were or had been cultivated, compared to the riparian corridor. Soil food web indices, based on functional analysis of nematode
faunal composition, reflected the aboveground landscape heterogeneity. Discriminant analysis indicated that soil food web
indices separated the two most disturbed habitats (ponds and tailwater ditches) from the two least disturbed habitats (the
riparian corridor and hedgerows). The indices correlated with soil functioning as inferred by soil properties. Abundance of
nematode taxa was not associated with aboveground landscape patterns. The complexity of the soil food web may have been influenced
by (1) environmental factors that differed between years, (2) different time periods since disturbance in the various habitats,
and (3) movement of nutrients and organisms by water flow between habitats in the farmscale. 相似文献
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Objective: To determine the antinociceptive effects of epidural administration of morphine or buprenorphine in cats by use of a thermal threshold model. ANIMALS: 6 healthy adult cats. PROCEDURES: Baseline thermal threshold was determined in duplicate. Cats were anesthetized with isoflurane in oxygen. Morphine (100 microg/kg diluted with saline [0.9% NaCl] solution to a total volume of 0.3 mL/kg), buprenorphine (12.5 microg/kg diluted with saline solution to a total volume of 0.3 mL/kg), or saline solution (0.3 mL/kg) was administered into the epidural space according to a Latin square design. Thermal threshold was determined at various times up to 24 hours after epidural injection. RESULTS: Epidural administration of saline solution did not affect thermal threshold. Thermal threshold was significantly higher after epidural administration of morphine and buprenorphine, compared with the effect of saline solution, from 1 to 16 hours and 1 to 10 hours, respectively. Maximum (cutout) temperature was reached without the cat reacting in 0, 74, and 11 occasions in the saline solution, morphine, and buprenorphine groups, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Epidural administration of morphine and buprenorphine induced thermal antinociception in cats. At the doses used in this study, the effect of morphine lasted longer and was more intense than that of buprenorphine. 相似文献
97.
Prevalences of various hemoplasma species among cats in the United States with possible hemoplasmosis 总被引:2,自引:0,他引:2
Sykes JE Terry JC Lindsay LL Owens SD 《Journal of the American Veterinary Medical Association》2008,232(3):372-379
OBJECTIVE: To determine prevalences of various hemoplasma species among cats in the United States with possible hemoplasmosis and identify risk factors for and clinicopathologic abnormalities associated with infection with each species. DESIGN: Cross-sectional study. Animals-310 cats with cytologic evidence of hemoplasmosis (n = 9) or acute or regenerative anemia (309). PROCEDURES: Blood samples were tested by means of a broad-spectrum conventional PCR assay for hemoplasma DNA and by means of 3 separate species-specific real-time PCR assays for DNA from "Candidatus Mycoplasma haemominutum" (Mhm), Mycoplasma haemofelis (Mhf), and "Candidatus Mycoplasma turicensis" (Mtc). RESULTS: Overall prevalences of Mhm, Mhf, and Mtc infection were 23.2% (72/310), 4.8% (15/310), and 6.5% (20/310), respectively. Mixed infections were detected in 20 (6.5%) cats. Cats infected with hemoplasmas were more likely to be male than were uninfected cats. Infection with FeLV or FIV was significantly associated with infection with Mhf. Compared with uninfected cats, cats infected with Mhf had higher reticulocyte counts, nucleated RBC counts, and mean corpuscular volume; cats infected with Mhm had higher mean corpuscular volume; and cats infected with Mtc had higher monocyte counts. CONCLUSIONS AND CLINICAL RELEVANCE: Results supported the suggestion that these 3 hemoplasma species commonly occur among cats in the United States and that pathogenicity of the 3 species varies. 相似文献
98.
Kiselow MA Rassnick KM McDonough SP Goldstein RE Simpson KW Weinkle TK Erb HN 《Journal of the American Veterinary Medical Association》2008,232(3):405-410
OBJECTIVE: To evaluate factors associated with response to treatment, remission duration, and survival in cats with low-grade lymphoma affecting various organ systems. DESIGN: Retrospective case series. Sample POPULATION: 41 cats with histologically confirmed low-grade lymphocytic lymphoma. PROCEDURES: Medical records and biopsy specimens of cats with histologically confirmed low-grade lymphocytic lymphoma of various organ systems treated with prednisone and chlorambucil between 1995 and 2005 were reviewed. The Kaplan-Meier method was used to estimate remission duration and survival. Factors potentially associated with prognosis were compared. RESULTS: Common clinical signs were weight loss (83%), vomiting (73%), anorexia (66%), and diarrhea (58%). Seventy-eight percent of cats tested had low serum cobalamin concentrations. Lymphoma was confined to the gastrointestinal tract in 68% of cats. Fifty-six percent of cats achieved a complete response to treatment, and 39% achieved a partial response. Five percent of cats had no response. No association was found between any risk factors (including anatomic site) and response to treatment. Partial response was associated with shorter remission duration, compared with complete response; median remission duration was 428 days for cats achieving a partial response, compared with 897 days for cats achieving a complete response. No other factors were associated with remission duration. Overall median survival time was 704 days. No factors were significantly associated with survival time. CONCLUSIONS AND CLINICAL RELEVANCE: Most cats with lymphocytic lymphoma responded to treatment with prednisone and chlorambucil, and most factors evaluated were not associated with outcome. 相似文献
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