Maternally and Naturally Acquired Antibody to Mannheimia haemolytica in Japanese Black Calves     

Konosuke OTOMARU  Sei KUBOTA  Makiko TOKIMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1675-1677

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Self-EcoTILLING to identify single-nucleotide mutations in multigene family   总被引：1，自引：0，他引：1  

Guang-Xi Wang  Toshiyuki Imaizumi  Wei Li  Hiromasa Saitoh  Ryohei Terauchi  Takanori Ohsako  Tohru Tominaga 《Pesticide biochemistry and physiology》2008,92(1):24-29

TILLING (Targeting Induced Local Lesions IN Genomes) is a low-cost, high-throughput reverse genetic technique that employs a mismatch-specific endonuclease CEL-1 to discover induced point mutations in the genes of interest. The use of the TILLING technique to survey natural variation in genes is called EcoTILLING. Here, we report a modified EcoTILLING method for the discovery of mutations in multigene family, which we coin “Self-EcoTILLING” by using an allotetraploid Monochoria vaginalis ALS multigene family as an example. The mutations could be detected by TILLING of PCR products resulting from the primers specific to both Als1 and Als3 without involving the experimental step of mixture of reference and query DNA. Either of the two co-amplified loci could serve as reference DNA to the other. We demonstrate with this example that Self-EcoTILLING is a fast, reliable and economical technique of detecting single-nucleotide mutations in polyploid plants containing multigene family.  相似文献   

Partial cDNA sequence of a relaxin‐like factor (RLF) receptor,LGR8 and possible existence of the RLF ligand‐receptor system in goat testes     

SIQIN  Mari NAKAI  Tomohiro HAGI  Shinichi KATO  Ali Mohammed PITIA  Mai KOTANI  Yuki ODANAKA  Yasushi SUGAWARA  Koh‐Ichi HAMANO  Keiichiro YOGO  Yoshio NAGURA  Masaru FUJITA  Hiroshi SASADA  Eimei SATO  Tetsuya KOHSAKA 《Animal Science Journal》2010,81(6):681-686

Relaxin‐like factor (RLF), also known as insulin‐like factor 3 (INSL3), is produced by testicular Leydig cells, but its specific receptor LGR8 (leucine‐rich repeat family of G‐protein‐coupled receptor 8) has not been identified in goats. This study aimed to identify complementary DNA (cDNA) sequences of goat LGR8, and characterize the expression of both RLF and LGR8 in goat testes by RT‐PCR and immunohistochemistry. Testes were collected from immature (3‐month‐old) and mature (24‐month‐old) Saanen goats, and partial cDNA sequences of the goat homologue of human LGR8 were identified. The sequence encoded a reduced peptide sequence of 167 amino acids, which corresponded to transmembrane regions 2 through 5, followed by the beginning of intracellular loop 3 of human LGR8. Expression of both LGR8 and RLF genes was drastically increased in mature testes compared with immature ones. Although RLF protein was restricted to Leydig cells, LGR8 protein was detected in both Leydig cells and seminiferous epithelial cells (possibly germ cells and Sertoli cells). These results reveal a possible existence of the RLF‐LGR8 ligand‐receptor system within the goat testis, suggesting that RLF may play a role in testicular function through LGR8 on Leydig cells and seminiferous epithelial cells in an autocrine and/or paracrine manner.  相似文献   

一株苯酚降解菌的筛选、鉴定及相关降解特性     

陈禹竹  唐琦勇  顾美英  朱静  刘晓静  崔卫东  张志东 《新疆农业科学》2019,56(10):1912-1920

【目的】筛选苯酚化合物相关降解微生物,确定其相关功能特性,为进一步揭示相关降解机制以及开发利用该菌株提供科学依据和基础。【方法】以苯酚为筛选压力对盐爪爪根际微生物进行分离筛选,采用16S rDNA序列测序确定相关降解菌的生物学地位,并对其抗逆特性和功能特性进行分析,开展其对苯酚和玉米赤霉烯酮降解的研究。【结果】获得了一株编号YZZ-9的菌株,经鉴定该菌为沼泽考克氏菌(Kocuria palustris),其能够在10%的NaCl,3.5 g/L的苯酚下生长良好,且能利用多种苯类化合物为单一碳源生长。当培养基中苯酚浓度低于1.0 g/L时,苯酚可在120 h内完全降解;当苯酚浓度达到1.5 g/L,培养基中的苯酚降解几乎完全被抑制。同时,菌株可明显降解玉米赤霉烯酮,当菌株培养144 h时,培养基中玉米赤霉烯酮降解率可达68.9%。【结论】菌株YZZ-9具有良好的苯酚和玉米赤霉烯酮降解能力。  相似文献   

Effects of smolt size and timing of migration on recovery rate of wild masu salmon <Emphasis Type="Italic">Oncorhynchus masou</Emphasis>     

Yasuyuki Miyakoshi  Sei-Ichi Saitoh 《Fisheries Science》2011,77(6):939-944

Little is known about the survival rate of wild masu salmon Oncorhynchus masou. To examine the effects of smolt length and migration timing on the recovery rate of wild masu salmon, we reanalyzed past tagging and recovery data (1993–1994). The tagging study was conducted in the Shokanbetsu River, northern Japan; 863 wild masu salmon smolts were captured, tagged, and released in a downstream site, and a total of 19 fish were recovered in coastal fisheries and in the natal river the following year. The data were analyzed by a logistic regression analysis with recapture as a response variable and tagging date and smolt length as explanatory variables; the tagging date had a significant effect on the recapture rate, whereas the effect of smolt size was not significant. Despite the small number of recaptures, this study indicates that migration timing is a factor affecting the marine survival of wild masu salmon smolts, although this conclusion has been repeatedly documented for other species of Pacific salmon Oncorhynchus spp.  相似文献   

Decreases in serum apolipoprotein C-III concentration in cows with ethionine-induced fatty liver     

Nitanai A  Katoh N  Oikawa S  Saitoh M  Hamada M  Kutsuzawa A  Mohamed T  Uchida E  Endoh D  Kurosawa T  Sato H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1113-1118

To monitor the serum concentration of apolipoprotein C-III (apoC-III), one of the functional apoproteins in lipid metabolism, in cows with ethionine-induced fatty liver, and to investigate the association of apoC-III with liver triglyceride (TG) content and serum biochemical variables, seven nonpregnant nonlactating Holstein cows (3 to 6 years old) were used. Five cows were treated with ethionine, an analogue of methionine, (days 0, 7 and 14). The remaining two controls received saline as the vehicle. Liver TG contents in the treated cows were increased markedly whenever administered, and significant increases were observed at days 14 (666.4%, 85.3 mg/g) and 21 (675.0%, 86.4 mg/g) compared with day 0. In controls, no significant changes in liver TG content and serum biochemical variables were observed during this experiment. The serum apoC-III concentration in the treated cows was decreased drastically after the first administration and fell to the lowest value at day 10 (76.2 microg/ml, 32% of day 0). The apoC-III was significantly (p<0.05) correlated with non-esterified fatty acids (r= -0.526), gamma-glutamyl transpeptidase (r= -0.407), total bilirubin (r= -0.464), positively with apolipoprotein B-100 (apoB-100, r=0.601) and cholesterol ester (r=0.449). Although apoB-100 concentrations were also reduced by the administrations, the concentrations tended to recover smoothly toward the next administration. The distinct difference in change between apoC-III and apoB-100 suggests that apoC-III may be regulated by other pathways, in addition to inhibiting the synthesis of apoproteins by ethionine.  相似文献   

Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos          下载免费PDF全文

Ken‐Ichi Yamanaka  Kyoko Yamashita  Hafiza Khatun  Yasuhiko Wada  Hideki Tatemoto  Miki Sakatani  Naoki Takenouchi  Masashi Takahashi  Shinya Watanabe 《Animal Science Journal》2018,89(10):1406-1414

Epigenetic reprogramming confers totipotency even during somatic cell nuclear transfer (SCNT), which has been used to clone various animal species. However, as even apparently healthy cloned animals sometimes have aberrant epigenetic status, the harmful effects of these defects could be passed onto their offspring. This is one of the biggest obstacles for the application of cloned animals for livestock production. Here, we investigated the DNA methylation status of four developmentally regulated genes (PEG3, XIST, OCT4, and NANOG) in sperms from a cloned and a non‐cloned bull, and blastocysts obtained by in vitro fertilization using those sperms and SCNT. We found no differences in the methylation status of the above genes between cloned and non‐cloned bull sperms. Moreover, the methylation status was also similar in blastocysts obtained with cloned and non‐cloned bull sperms. In contrast, the methylation status was compromised in the SCNT blastocysts. These results indicate that sperm from cloned bulls would be adequately reprogrammed during spermatogenesis and, thus, could be used to produce epigenetically normal embryos. This study highlights the normality of cloned bull offspring and supports the application of cloned cattle for calf production.  相似文献   

Aberrant expression of microRNAs and the miR‐1/MET pathway in canine hepatocellular carcinoma          下载免费PDF全文

Y.‐C. Lai  N. Ushio  M. M. Rahman  Y. Katanoda  K. Ogihara  Y. Naya  A. Moriyama  T. Iwanaga  Y. Saitoh  T. Sogawa  T. Sunaga  Y. Momoi  H. Izumi  N. Miyoshi  Y. Endo  M. Fujiki  H. Kawaguchi  N. Miura 《Veterinary and comparative oncology》2018,16(2):288-296

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Molecular biological studies on appressorium formation in the rice blast fungus     

Ken-ichiro Saitoh 《Journal of General Plant Pathology》2010,76(6):419-420

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Microtubule-severing activity of Shigella is pivotal for intercellular spreading   总被引：1，自引：0，他引：1  

Yoshida S  Handa Y  Suzuki T  Ogawa M  Suzuki M  Tamai A  Abe A  Katayama E  Sasakawa C 《Science (New York, N.Y.)》2006,314(5801):985-989

Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its alpha-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.  相似文献