Structural characterization and hypoglycemic effects of arabinogalactan-protein from the tuberous cortex of the white-skinned sweet potato (Ipomoea batatas L.)     

Ozaki S  Oki N  Suzuki S  Kitamura S 《Journal of agricultural and food chemistry》2010,58(22):11593-11599

An arabinogalactan-protein (WSSP-AGP) was isolated from the tuberous cortex of the white-skinned sweet potato (WSSP; Ipomoea batatas L.). It consists of 95% (w/w) carbohydrate and 5% (w/w) protein with high contents of hydroxyproline, alanine, and serine. Its sugar composition is α-L-Rha:α-L-Ara:β-D-Gal:β-D-GlcA in a molar ratio of 1.0:4.1:7.6:1.3. Its weight-average molecular weight was estimated to be 126,800 g/mol by high-performance size exclusion chromatography coupled with multiangle laser light scattering. Structural analysis indicated that WSSP-AGP is a (1→3)-β-D-galactan highly branched at O-6 with (1→6)-β-D-galactan, in which the branched chains are substituted at the O-3 position with α-L-Araf-(1→ and α-L-Araf-(1→5)-α-L-Araf-(1→ and at the O-6 position typically with α-L-Rhap-(1→4)-β-D-GlcAp-(1→ as terminating groups. Continuous administration of WSSP-AGP to KKAy mice significantly lowered fasting plasma glucose levels. This indicates that WSSP-AGP plays an important role in the hypoglycemic effects of WSSP.  相似文献   

Molecular cloning and characterization of oryzacystatin-III, a novel member of phytocystatin in rice (Oryza sativa L. japonica)     

Ohtsubo S  Kobayashi H  Noro W  Taniguchi M  Saitoh E 《Journal of agricultural and food chemistry》2005,53(13):5218-5224

On the basis of cDNA sequences, we found that the calli of rice encodes an amino acid sequence that shares 56% and 89% identity, respectively, with oryzacystatin-I and oryzacystatin-II. This sequence differs from that of oryzacystatin-II in the N-terminal region (Gln(7)-Ala(19) in the oryzacystatin-III numbering), and this region contained a glycine residue (Gly(14)), which is evolutionarily conserved in the cystatin superfamily. We named this novel protein oryzacystatin-III. Nucleotide sequencing of the 5'-flanking region of the oryzacystatin-III gene showed that it is highly homologous to the oryzacystatin-II gene but distinct from the oryzacystatin-II locus. Oryzacystatin-III inhibited papain, ficin, and human cathepsin B. The inhibition constants for papain and ficin differ from those of oryzacystatin-I and -II, and cathepsin B activity is affected only by oryzacystatin-III, showing differences in the interaction of these inhibitors with enzymes. These data suggest that the above three inhibitors may play unique physiological roles in the regulations of rice cysteine proteinases.  相似文献   

Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish     

N Nose  Y Hoshino  Y Kikuchi  M Horie  K Saitoh  T Kawachi  H Nakazawa 《Journal of the Association of Official Analytical Chemists》1987,70(4):714-717

A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran-acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 microL injection.  相似文献   

Behavior of Iodine in a Forest Plot, an Upland Field and a Paddy Field in the Upland Area of Tsukuba, Japan. Iodine Concentration in Precipitation, Irrigation Water, Ponding Water and Soil Water to a Depth of 2.5 m     

Kouichi Yuita  Nobuharu Kihou  Shiho Yabusaki  Yoshio Takahashi  Takayuki Saitoh  Akito Tsumura  Hideki Ichihashi 《Soil Science and Plant Nutrition》2005,51(7):1011-1021

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Distribution of Staphylococcus species on animal skin.     

A Shimizu  J Ozaki  J Kawano  Y Saitoh  S Kimura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(2):355-357

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Amyloid beta protein precursor is possibly a heparan sulfate proteoglycan core protein   总被引：16，自引：0，他引：16  

D Schubert  R Schroeder  M LaCorbiere  T Saitoh  G Cole 《Science (New York, N.Y.)》1988,241(4862):223-226

The amyloid beta protein peptide is a major constituent of amyloid plaque cores in Alzheimer's disease and is apparently derived from a higher molecular weight precursor. It is now shown that the core protein of a heparan sulfate proteoglycan secreted from a nerve cell line (PC12) has an amino acid sequence and a size very similar to those of the amyloid beta protein precursor and that these molecules are antigenically related. This amyloid beta protein precursor-related protein is not found in the conditioned medium of a variant cell line (F3 PC12) that does not secrete heparan sulfate proteoglycan. The synaptic localization and metabolism of this class of proteoglycans are consistent with its potential involvement in central nervous system dysfunction.  相似文献   

Rejection of renal allografts: specific immunologic suppression   总被引：17，自引：0，他引：17  

F P Stuart  T Saitoh  F W Fitch 《Science (New York, N.Y.)》1968,160(835):1463-1465

Kidneys were transplanted across a major genetic barrier (Ag-B locus), from LewisxBN F(1) hybrid rats into bilaterally nephrectomized Lewis rats. Survival of grafts is prolonged (indefinite?) in rats treated with a combination of (i) intravenous injection of donor spleen cells 1 day before the graft, and (ii) passive immunization with antiserum prepared in rats of the recipient strain against donor spleen and lymph-node cells. The recipient's immune response to other antigens is not impaired.  相似文献   

Identification of <Emphasis Type="Italic">Undaria pinnatifida</Emphasis> and <Emphasis Type="Italic">Undaria undarioides</Emphasis> Laminariales,Phaeophyceae using mitochondrial 23S ribosomal DNA sequences     

Daisuke?MuraokaEmail author  Kenji?Saitoh 《Fisheries Science》2005,71(6):1365-1369

ABSTRACT:   Mitochondrial 23S ribosomal (r) DNA were sequenced from two Undaria species. The 23S rDNA from seven Undaria pinnatifida individuals were 2707 bp in length, whereas the 23S rDNA from four Undaria undarioides individuals were 2708–2709 bp in length. We found 15–20 nucleotide substitutions and 1–2 gaps between U. pinnatifida (the major haplotype) and U. undarioides . Based on the differences in sequences, we carried out PCR/RFLP analyses to distinguish between U. pinnatifida and U. undarioides , which showed different PCR/RFLP patterns upon Hin fI digestion. Sequence differences and PCR/RFLP analyses of mt 23S rDNA are useful for species identification of Undaria species .  相似文献   

Expression of Connexin 43 in the Porcine Foetal Gonads During Development     

K Knapczyk–Stwora  M Durlej–Grzesiak  M Duda  M Slomczynska 《Reproduction in domestic animals》2013,48(2):272-277

This study was designed to reveal connexin 43 (Cx43) mRNA and protein expression in porcine foetal gonads using RT‐PCR, immunohistochemistry and Western blot analysis. Expression of Cx43 was investigated in porcine foetal ovaries and testes on days 50, 70 and 90 post coitum (p.c.). RT‐PCR results indicated that Cx43 mRNA was expressed in both foetal ovaries and testes at all gestational ages examined. Cx43 protein was found in the foetal ovary but its distribution varied across ovarian compartments and changed during development. In foetal ovaries, Cx43 was localized between the interstitial cells surrounding egg nests on all investigated days of prenatal period. Moreover, Cx43 expression was observed between germ cells on day 50 p.c. as well as between pre‐granulosa and granulosa cells of primordial and primary follicles on days 70 and 90 p.c. In the foetal testes, Cx43 protein was detected between neighbouring Leydig cells on all examined days of prenatal period and between adjacent Sertoli cells exclusively on day 90 p.c. The presence of Cx43 protein in all investigated foetal gonads was confirmed by Western blot analysis. Cx43 protein detection between pre‐granulosa cells of primordial follicles suggests its role in regulation of the initial stages of follicle development. The Cx43 immunoexpression between neighbouring Leydig and between Sertoli cells indicates its involvement in controlling their functions. We propose that Cx43‐mediated gap junctional communication is involved in the regulation of porcine foetal gonadal development.  相似文献   

Dataset of CarboEastAsia and uncertainties in the CO2 budget evaluation caused by different data processing     

Nobuko Saigusa  Sheng-Gong Li  Hyojung Kwon  Kentaro Takagi  Lei-Ming Zhang  Reiko Ide  Masahito Ueyama  Jun Asanuma  Young-Jean Choi  Jung Hwa Chun  Shi-Jie Han  Takashi Hirano  Ryuichi Hirata  Minseok Kang  Tomomichi Kato  Joon Kim  Ying-Nian Li  Takahisa Maeda  Akira Miyata  Yasuko Mizoguchi  Shohei Murayama  Yuichiro Nakai  Takeshi Ohta  Taku M. Saitoh  Hui-Ming Wang  Gui-Rui Yu  Yi-Ping Zhang  Feng-Hua Zhao 《Journal of Forest Research》2013,18(1):41-48

The datasets of net ecosystem CO₂ exchange (NEE) were acquired from 21 forests, 3 grasslands, and 3 croplands in the eastern part of Asia based on the eddy covariance measurements of the international joint program, CarboEastAsia. The program was conducted by three networks in Asia, ChinaFLUX, JapanFlux, and KoFlux, to quantify, synthesize, and understand the carbon budget of the eastern part of Asia. An intercomparison was conducted for NEE estimated by three gap-filling procedures adopted by ChinaFLUX, JapanFlux, and KoFlux to test the range of uncertainty in the estimation of NEE. The overall comparison indicated good agreement among the procedures in the seasonal patterns of NEE, although a bias was observed in dormant seasons depending on the different criteria of data screening. Based on the gap-filled datasets, the magnitude and seasonality of the carbon budget were compared among various biome types, phenology, and stress conditions throughout Asia. The annual values of gross primary production and ecosystem respiration were almost proportional to the annual air temperature. Forest management, including clear-cutting, plantation, and artificial drainage, was significant and obviously affected the annual carbon uptake within the forests. Agricultural management resulted in notable seasonal patterns in the crop sites. The dataset obtained from a variety of biome types would be an essential source of knowledge for ecosystem science as well as a valuable validation dataset for modeling and remote sensing to upscale the carbon budget estimations in Asia.  相似文献