Influence of Hatchery Protocols on Mitochondrial DNA Variation in Japanese Flounder Juveniles   总被引：1，自引：0，他引：1  

Takashi  Asahida Yumi  Shinotsuka Unji  Saitoh Yoh  Yamashita Hitoshi  Ida Ken-Ichi  Hayashizaki 《Journal of the World Aquaculture Society》2003,34(2):121-132

The Japanese flounder Paralichthys olivaceus is a commercially important fish that is stocked extensively from hatchery rearing programs in Japan. To examine the genetic variability of hatchery-raised juveniles of the Japanese flounder that are used for stocking into natural waters, we analyzed a portion of the mitochondrial genome. The mtDNA region extending from the 3'half of the cytochrome b gene to the central domain of the control region was PCR amplified and analyzed using 11 restriction endonucleases. We identified 34 polymorphic cleavage sites out of a total of 61 sites, which resulted in 67 different haplotypes in a total of 265 offspring, examined from eight hatchery stocks. Haplotype diversity of offspring at each of the eight hatcheries ranged from 0.49 ± 0.09 (SE) to 0.94 ± 0.03 (SE). Also, we observed 40 polymorphic sites out of a total of 59 sites, which resulted in a total of 50 haplotypes in 60 wild flounder. Haplotype diversity of the wild population was 0.98 ± 0.01 (SE). The use of subcultured fishes as broodstock appears to be one of the most important causes of reduced genetic diversity in hatchery-raised flounder juvenile. Our results suggest that the use of wild fish for broodstock is an effective way to maintain genetic variability in Japanese flounder offspring.  相似文献   

Preface for the SUITMA10 Conference proceedings Soils of Urban,Industrial, Traffic,Mining, and Military Areas (SUITMAs) are essential resources that deserve our attention for the sustainable management of anthropized environments     

Kim  K–H. J.  Morel  J. L.  Nehls  T.  Charzynski  P.  Levin  M. J.  Vasenev  V. I. 《Journal of Soils and Sediments》2021,21(5):1907-1908

Journal of Soils and Sediments -  相似文献   

Molecular Cloning,Characterization of Porcine IZUMO1, an IgSF Family Member     

E Kim  J‐S Kim  Y Lee  B‐S Song  B‐W Sim  S‐U Kim  T Saitoh  H Yazawa  T Nunoya  K‐T Chang 《Reproduction in domestic animals》2013,48(1):90-97

IZUMO1, belonging to the family of mammalian immunoglobulin proteins, has been well characterized in the mouse. Here, we describe the molecular cloning and expression analysis of porcine IZUMO1 (pIZUMO1). Partial sequence information published in the National Center for Biotechnology Information (NCBI) database was used to generate the full‐length sequence for IZUMO1 using rapid amplification of cDNA ends (RACE). A search of the porcine genomic sequence in the NCBI database identified a bacterial artificial chromosome (BAC) encoding the pIZUMO1 gene. This BAC is derived from porcine chromosome 6 and is syntenic with the corresponding regions of mouse, bovine, and human genomes encoding the IZUMO gene family. This BAC was found to encode an IZUMO1 protein with a predicted amino acid sequence having high similarity with mouse and human IZUMO1. Western blot analysis of proteins from porcine tissues indicated that pIZUMO1 was specifically expressed in the sperm. Furthermore, to confirm whether pIZUMO1 forms complexes, we overexpressed pIZUMO1 in HEK293 cells. The recombinant pIZUMO1 from cell extracts was found to form complexes. Our finding suggests that pIZUMO1 forms homodimeric complex on the sperm membrane. Furthermore, an IVF inhibition assay with an antibody for the porcine IZUMO1 Ig‐like domain showed that Ig‐like domain effectively prevented pig sperm–egg interactions.  相似文献   

Molekulargenetische Analyse des ‘Maschanzker’/‘Borsdorfer’‐Sortenkomplexes     

Alberto Storti  Hans–Joachim Bannier  Christian Holler  Bernd Kajtna  Thomas Rühmer  Alois Wilfling  Claudio Soldavini  Josef Dalla Via  Sanja Baric 《Erwerbs-Obstbau》2013,55(3-4):99-107

‘Maschanzker’ is one of the best-known old apple cultivars in Austria and in the 19th century this variety also played an important role in the fruit production of South Tyrol (Northern Italy). ‘Maschanzker’ is believed to originate from one of the oldest German apple vari­eties ‘Borsdorfer’, which is even considered a synonym. However, since the denomination ‘Maschanzker’ is part of the name of a number of more or less similar types of apple, this study aimed at investigating the synonymy and homonymy as well as the relationships within and between different accessions of ‘Maschanzker’, ‘Borsdorfer’ and ‘Edelborsdorfer’ by employing 14 microsatellite DNA markers. A total of 38 accessions from several collections in Europe were analysed and 14 different genotypes were identified, which were assigned to three groups. Our data suggest a series of parent-offspring connections within the here defined Maschanzker group and the Edelborsdorfer group, while no closer relationship was evident to or among the accessions of the Borsdorfer group. Molecular genetic analyses thus provide fundamental data which can serve as a basis for the pomological revision of the ‘Maschanzker’/‘Borsdorfer’ cultivar complex.  相似文献   

Glomerulopathy in a cat with cyanotic congenital heart disease     

K Shirota  Y Saitoh  Y Une  Y Nomura 《Veterinary pathology》1987,24(3):280-282

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Dung beetles, Onthophagus spp., as potential transport hosts of feline coccidia     

Y Saitoh  H Itagaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):293-297

Cockroaches and filth-flies have been known to be transport hosts of Toxoplasma gondii but the role of dung beetles as the carrier of coccidian oocysts is not known. We attempted to clarify the role of dung beetles (Onthophagus spp.) as the transport host of feline coccidia including Toxoplasma. Toxoplasma oocysts were found in the feces of the beetles until day 3 after the insects were exposed to cat feces mixed with the oocysts. Furthermore, oocysts on the body surface of beetles were not easily detached but remained infective for a prolonged period of time. Infective dung beetles may contaminate the water with infective oocysts passed in their feces when they dropped into the water. In the field survey feline coccidia, Isospora felis and I. rivolta, were detected in dung beetles collected from dog feces; they play an important role in the transmission of feline coccidian oocysts in the field.  相似文献   

Expression in Escherichia coli and purification of the functional feline granulocyte colony-stimulating factor     

Yamamoto A  Iwata A  Saitoh T  Tuchiya K  Kanai T  Tsujimoto H  Hasegawa A  Ishihama A  Ueda S 《Veterinary immunology and immunopathology》2002,90(3-4):169-177

Feline granulocyte colony-stimulating factor (G-CSF) with an N-terminal histidine hexamer tag was expressed as inclusion bodies in E. coli. The G-CSF solubilized in 6 M guanidine solution was absorbed onto a Ni-NTA column and, after washing with decreasing concentrations of guanidine, eluted with imidazole in a soluble and apparently pure form. The activity of the recombinant feline G-CSF was 3×10⁶ U/mg protein, as assayed by its stimulatory effect on NFS-60 cell proliferation. When a low level of purified feline G-CSF was administered once a day for two successive days to cats, the number of neutrophil increased 4-fold while the levels of other blood cell types remained virtually unchanged. Daily administration of G-CSF for a total of 11 days led to a more than 10-fold increase in neutrophils, an 8-fold increase in the number of monocytes and 2-fold increase in lymphocytes. No severe side effects or antibody production was observed in cats after administration of G-CSF.  相似文献   

Evaluation of nafamostat mesilate as an alternative anticoagulant during intermittent hemodialysis in healthy Beagle dogs          下载免费PDF全文

Joon‐hyuk Choi DVM  MS  Seok‐yeong Byun DVM  MS  Aryung Nam DVM  Sei‐myoung Han DVM  PhD  Kyu‐pil Lee DVM  PhD  Kun‐ho Song DVM  PhD  Hwa‐young Youn DVM  PhD  Kyoung‐won Seo DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2018,28(2):122-129

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Phosphorus-induced zinc deficiency in wheat pot-grown on noncalcareous and calcareous soils of different properties     

Daniel Sacristán  Adrian González–Guzmán  Vidal Barrón  José Torrent 《Archives of Agronomy and Soil Science》2019,65(2):208-223

High levels of phosphorus (P) often induce zinc (Zn) deficiency in plants grown on Zn-poor soils. We investigated P-induced Zn deficiency in durum wheat (Triticum durum L. ‘Carpio’) grown on 16 noncalcareous and 31 calcareous soils differing in levels of available (Olsen) P and available (diethylenetriaminepentaacetic acid (DTPA)-extractable) Zn using micropots. A completely randomized factorial design with two levels of P (0 and 40 mg P kg^?1 soil) and Zn (0 and 3 mg Zn kg^?1 soil), i.e. four treatments (‘control’, + P, + Zn, and + PZn), were used. Grain yield of control plants depended mainly on the Olsen P level. Phosphorus had a negative effect on yield in 6 soils with Olsen P/Zn_DTPA > 25, and Zn a positive one in 5 soils with Olsen P/Zn_DTPA > 50; and the + PZn treatment generally resulted in the highest yield. Grain Zn concentration of control plants was negatively correlated with growth and Olsen P. Calcareous soils were less sensitive to P-induced Zn deficiency than noncalcareous soils because phosphate is sorbed by calcite rather than being co-adsorbed with Zn on the Fe oxides. Co-application of P and Zn to soil at low and application of Zn at high Olsen P ensured both maximum yield and grain Zn bioavailability.  相似文献   

Interaction between grapevines and trees: effects on water relations,nitrogen nutrition,and wine     

Carina P. Lang  Nikolaus Merkt  Christoph-Martin Geilfus  Simone Graeff–Hönninger  Judy Simon  Heinz Rennenberg 《Archives of Agronomy and Soil Science》2019,65(2):224-239

Agroforestry systems (AF) consisting of grapevines and trees, may lead to resource competition for water and nutrients. This study aimed to evaluate the impact of a combined cultivation on water relations, nitrogen nutrition and the resulting wine quality.

¹⁵N-labeled inorganic nitrogen (N) sources were used to quantify net N uptake capacity. N content and δ¹⁵N natural abundance were analysed as integrating parameters of N nutrition. Leaf water potential (ψ_leaf) was determined to evaluate the water status of grapevines. Wine quality was evaluated by chemical and sensory analyses. In result, AF system reduced leaf water potential and increased net N uptake capacity in grapevines. However, chemical composition and sensory quality of the wine were not significantly affected in the present system consisting of Riesling, Sauvignon Blanc, oak and poplar.

Nitrogen availability of grapevines was favourable and water relations were improved, whereas wine quality was similar when grown with trees or without. Trees were able to reduce water and nitrogen losses without negative effects on wine quality.

This work provides information on benefits and limits for intercropping of trees and grapevines in terms of performance of grapevines and wine quality compared to traditional vineyard systems.  相似文献