Dietary L-carnitine increases plasma insulin-like growth factor-I concentration in chicks fed a diet with adequate dietary protein level   总被引：2，自引：0，他引：2  

Kita K  Kato S  Amanyaman M  Okumura J  Yokota H 《British poultry science》2002,43(1):117-121

1. The effect of L-carnitine supplemented into experimental diets with varying dietary protein concentrations (50, 200 and 400 g/kg) on body weight gain and plasma insulin-like growth factor-I (IGF-I) concentration in chicks was examined. 2. Dietary L-carnitine supplementation provided 0, 200, 500 and 1000 mg/kg. Chicks were given the diet ad libitum for 10 d. 3. When L-carnitine was provided as 500 or 1000 mg/kg, body weight gain was significantly improved in birds receiving the 200 and 400 g protein/kg diets. 4. There was an interaction between dietary L-carnitine and protein content on plasma IGF-I concentration. L-carnitine supplementation had little influence on plasma IGF-I concentrations in birds receiving the low protein (50 g/kg) diet. When dietary L-carnitine concentrations were increased from 0 to 1000 mg/kg in the adequate protein (200 g/kg) diet, plasma IGF-I concentrations were also increased. However, when dietary L-carnitine content was more than 500 mg/kg in the 400 g/kg protein group, plasma IGF-I concentration decreased with increasing dietary L-carnitine content. 5. Body weight change correlated significantly with the alteration in plasma IGF-I concentrations in chicks given diets with adequate dietary protein. 6. In conclusion, the improvement in body weight gain caused by dietary L-carnitine supplementation was achieved when chicks were given their dietary protein requirement, which may be partially explained by an increase in plasma IGF-I concentration.  相似文献   

Effects of dietary cholestyramine on the utilisation of diets containing medium or long chain triacylglycerols by chicks     

M. Furuse  R. T. Mabayo  J. Okumura 《British poultry science》1997,38(4):436-438

1. The effects of graded dietary concentrations of cholestyramine (CSTY, a bile acid binding polymer), which prevents micelle formation and bile acid reabsorption, on the lipid and energy metabolism of chicks given diets containing different dietary concentrations of medium chain triacylglycerol (MCT) and long chain triacylglycerol (LCT) were investigated.

2. MCT‐ or LCT‐supplemented diets containing 100 or 200 g oil/kg diet and 0, 10 or 20 g CSTY kg were fed to 7 d old chicks for 10 d. As dietary CSTY concentration increased, a reduction in the metabolisable energy value was observed for both dietary lipid sources. Consequently, fat and energy retentions were also reduced as the dietary CSTY content increased.  相似文献   

Ovalbumin mRNA in the magnum of laying hens changes during egg formation cycle     

T. muramatsu  H. Hiramatsu  J. Okumura 《British poultry science》1994,35(3):457-461

  相似文献   

Protein synthesis in the liver and breast muscle of chicks fed on a high protein diet     

K. Kita  J. Okumura 《British poultry science》1993,34(3):553-558

1. The influence of feeding a high protein diet containing 400 g crude protein (CP)/kg on liver and breast muscle protein synthesis in chicks was investigated using a large dose injection of l‐[4 ‐³H]phenylalanine.

2. Liver weight of chicks fed on the high protein diet was significantly heavier than that of chicks fed on a control diet with 200 g CP/kg, and breast muscle weight was not affected by the treatment.

3. Fractional and absolute protein synthesis rates, RNA and protein contents, and RNA:protein ratio of breast muscle were not influenced by feeding the high protein diet.

4. Feeding the high protein diet decreased fractional synthesis rate of liver protein, an observation explained by reduced RNA:protein ratio. Liver protein content was elevated by feeding the high protein diet and this resulted in no change in the absolute synthesis rate of liver protein.  相似文献   

Production efficiency and telomere length of the cloned pigs following serial somatic cell nuclear transfer   总被引：2，自引：0，他引：2  

Kurome M  Hisatomi H  Matsumoto S  Tomii R  Ueno S  Hiruma K  Saito H  Nakamura K  Okumura K  Matsumoto M  Kaji Y  Endo F  Nagashima H 《The Journal of reproduction and development》2008,54(4):254-258

The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the starting nuclear donor cells, porcine salivary gland progenitor cells were collected from the resultant first-generation cloned pigs to successively produce second- and third-generation clones, with no significant differences in production efficiency, which ranged from 1.4% (2/140) to 3.3% (13/391) among the 3 generations. The average telomere lengths (terminal restriction fragment values) for the first, second and third generation clones were 16.3, 18.1 and 20.5 kb, respectively, and were comparable to those in age-matched controls. These findings suggest that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pigs from the first to third generations are normal.  相似文献   

Association between exogenous atrial natriuretic peptide and hemodynamics in dogs with congestive heart failure produced by experimental mitral regurgitation   总被引：2，自引：0，他引：2  

Asano K  Masuda K  Okumura M  Kadosawa T  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):243-250

Association between exogenous atrial natriuretic peptide (ANP) and hemodynamic changes was ascertained in 3 dogs with overt congestive heart failure (CHF(+)) and 3 dogs without congestive heart failure (CHF(-)) caused by experimental mitral regurgitation (MR). The hemodynamic measurements were recorded in all dogs during and after 1 hr infusion of ANP at the rate of 0.1 (low dose), 0.5 (medium dose) and 1.0 (high dose) microg/kg/min, respectively. Heart rate, mean arterial pressure, pulmonary capillary wedge pressure (PCWP) and systemic vascular resistance decreased significantly during and after ANP infusion even with low dose in the CHF(+). Stroke volume, stroke volume index and cardiac output in the CHF(+) during and after ANP infusion showed an increasing trend as compared with the CHF(-). Double product, an indicator of myocardial oxygen consumption, significantly decreased during and after ANP administration at all doses in the CHF(+). These findings indicate that even at low dose, exogenous ANP improves cardiac performance and reduces myocardial oxygen consumption in the CHF(+), and suggest that ANP has beneficial effects in the treatment of dogs with overt congestive heart failure resulting from MR.  相似文献   

Acute respiratory failure caused by leukaemic infiltration of the lung of a dog     

Mori T  Kadosawa T  Okada Y  Ochiai K  Umemura T  Tanoue S  Okumura M  Fujinaga T 《The Journal of small animal practice》2001,42(7):349-351

A seven-year-old crossbred male dog with a suspected leukaemic condition was referred for investigation and treatment. A bone marrow aspirate revealed an acute myeloid leukaemia. Combination chemotherapy was administered and the dog initially improved, but 18 days after the initiation of therapy its body condition deteriorated and the animal developed acute respiratory distress. On postmortem examination, extensive leukaemic pulmonary infiltrates were evident.  相似文献   

Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd     

Nguyen Thi Trang  Takuya Hirai  Tsukasa Yamamoto  Mari Matsuda  Naoko Okumura  Nguyen Thi Huong Giang  Nguyen Thi Lan  Ryoji Yamaguchi 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):361-367

  相似文献   

Spherical crystallization: direct spherical agglomeration of salicylic Acid crystals during crystallization     

Kawashima Y  Okumura M  Takenaka H 《Science (New York, N.Y.)》1982,216(4550):1127-1128

Direct spherical agglomeration of salicylic acid crystals during crystallization is described. The needle-like salicylic acid crystals simultaneously form and agglomerate in a mixture of three partially miscible liquids, such as water, ethanol, and chloroform, with agitation. The agglomerates can be made directly into tablets because of their excellent flowability. Spherical crystallization could eliminate the usual separate agglomeration step after crystallization and may be adaptable to other pharmaceutical and chemical systems.  相似文献   

Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale     

Higaki-Sato N  Sato K  Esumi Y  Okumura T  Yoshikawa H  Tanaka-Kuwajima C  Kurata A  Kotaru M  Kawabata M  Nakamura Y  Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13

An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified.  相似文献