Effects of treatment with polysulfated glycosaminoglycan on serum cartilage oligomeric matrix protein and C-reactive protein concentrations, serum matrix metalloproteinase-2 and -9 activities, and lameness in dogs with osteoarthritis   总被引：1，自引：0，他引：1  

Fujiki M  Shineha J  Yamanokuchi K  Misumi K  Sakamoto H 《American journal of veterinary research》2007,68(8):827-833

OBJECTIVE: To investigate the effects of polysulfated glycosaminoglycan (PSGAG) treatment on serum cartilage oligomeric matrix protein (COMP) concentration, matrix metal-loproteinase-2 (MMP-2) and -9 (MMP-9) activities, C-reactive protein (CRP) concentration, and lameness scores in dogs with osteoarthritis. ANIMALS: 16 dogs with osteoarthritis and 5 clinically normal dogs. PROCEDURES: Dogs with osteoarthritis had a history of chronic lameness, and osteophytes were observed on radiographic evaluation of the affected joint. Polysulfated glycosaminoglycan was administered IM twice a week for a total of 8 treatments to all dogs with osteoarthritis and to clinically normal control dogs. RESULTS: Lameness scores after PSGAG treatment in osteoarthritic dogs improved in 12 of the 16 dogs. Serum COMP concentrations in osteoarthritic dogs were significantly higher than in control dogs before treatment. Lameness scores in osteoarthritic dogs decreased significantly after treatment, compared with before treatment. Lameness scores of 9 dogs with hind limb lameness improved significantly after treatment; these dogs had corresponding decreases in serum COMP concentrations. After treatment, serum COMP concentrations and lameness scores of 7 dogs with forelimb lameness remained high and were significantly higher than those of dogs with hind limb lameness. Serum MMP-9 activities of dogs with forelimb lameness were significantly higher than in dogs with hind limb lameness after treatment. CONCLUSIONS AND CLINICAL RELEVANCE: IM administration of PSGAG inhibited COMP degradation in dogs with osteoarthritis. Results indicate that decreases in serum COMP concentrations might be related to improvement in lameness after PSGAG treatment.  相似文献   

The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle          下载免费PDF全文

Kiri Hayakawa  Takayuki Sakamoto  Atsushi Ishii  Keita Yamaji  Yoshinobu Uemoto  Nanae Sasago  Eiji Kobayashi  Naohiko Kobayashi  Tamako Matsuhashi  Shin Maruyama  Hirokazu Matsumoto  Kenji Oyama  Hideyuki Mannen  Shinji Sasazaki 《Animal Science Journal》2015,86(8):737-746

The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non‐synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome‐wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome‐wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high‐grade beef as a selection marker in beef cattle.  相似文献   

A modeling approach for assessing rice cropping cycle affected by flooding,salinity intrusion,and monsoon rains in the Mekong Delta,Vietnam     

Akihiko Kotera  Khang Duy Nguyen  Toshihiro Sakamoto  Toshichika Iizumi  Masayuki Yokozawa 《Paddy and Water Environment》2014,12(3):343-354

We developed a crop scheduling model for rice cultivation in the Vietnam Mekong Delta (VMD), focusing on the adaptive behavior of crop planning to various water resource constraints. In addition, we also examined the effects of environmental change on rice cultivation in the last decade. In the VMD, multiple rice cropping is practiced under a variety of adverse water conditions, including flooding, salinity intrusion, and irregular monsoon rains. These environmental changes influence the durations of growing seasons and the number of crops per year, resulting in changes in productivity. To validate the performance of the model, we compared model estimates for the heading date and changes in leaf area index at nine sites with estimates of these parameters derived from MODIS satellite time series data for the period 2002–2006. The root mean square errors of heading date between the modeled and satellite data in the upper, middle, and coastal regions of the delta were 17.6, 11.2, and 13.0 days, respectively. Based on the model, we examined case studies to assess the changes in cropping cycles and crop failures in the VMD due to extreme flooding in 2000 and salinity intrusion in 2004 by applying evaluation indices defined by available period for cultivation (APC) and safe margin for cropping (SMC) which is defined as the marginal time between APC and the period required for cultivation. Findings of case studies suggested that a small difference in the SMC of the cropping pattern is critical to the stability and productive capacity of the rice crop.  相似文献   

Comparison of Ammonium Deposition Flux at Roadside and at an Agricultural Area for Long-Term Monitoring: Emission of Ammonia from Vehicles     

Rie Matsumoto  Natsumi Umezawa  Masafumi Karaushi  Shin-Ichi Yonemochi  Kazuhiko Sakamoto 《Water, air, and soil pollution》2006,173(1-4):355-371

To understand the effect of the NH₃ emissions from vehicles, the NH₄ ⁺ bulk deposition and concentrations of gaseous NH₃ and particulate NH₄ ⁺ were measured at two sites in Saitama prefecture, Japan, one at the roadside of an arterial road (Kounosu), and the other in an agricultural area (Kisai). We observed that the NH₄ ⁺ bulk deposition and NH₃ concentration were significantly increased in Kounosu; the effect of NH₃ emissions from vehicles was confirmed. NOx was a primary pollutant mainly emitted by vehicle exhaust in the vicinity of arterial roads, and the relationship between NO _x and sum of gaseous NH₃ and particulate NH₄ ⁺ (T-NH₄ ⁺) was examined. The [T-NH₄ ⁺/NO _x ] ratios in Kounosu ranged from 0.08 to 0.23 (average 0.15), and in Kisai they ranged from 0.16 to 0.67 (average 0.39). In Kounosu, the effect of vehicle exhaust was great, but seasonal variation was small. The contribution ratio of NH₃ from vehicle emissions increased at low temperatures, while that from the non-vehicle emissions increased at high temperatures.  相似文献   

Dual inoculation of peanut with Glomus sp. and Bradyrhizobium sp. enhanced the symbiotic nitrogen fixation as assessed by 15N-Technique     

Monowar Karim Khan  Kazunori Sakamoto  Tomio Yoshida 《Soil Science and Plant Nutrition》2013,59(4):769-779

Abstract

The response of peanut (Arachis hypogaea L.) to inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi (Glomus etunicatum) and Bradyrhizobiurn sp. was studied in pots by the acetylene reduction activity (ARA) and ‘A-value’ methods. The soil used was a Light-coloured Andosol and the treatments consisted of the inoculation of VAM fungi only, inoculation of Bradyrhizobium only, dual inoculation of VAM fungi and Bradyrhizobium and control, under non-sterilized and sterilized soil conditions.

In the non-sterilized soil the ARA and nitrogen fixation determined by the ‘A-value’ method increased significantly only by dual inoculation of VAM fungi and Bradyrhizobium at 100 days after planting (DAP), but no significant difference was observed at 70 DAP. In the case of dual inoculation, 75% of the nitrogen of the plant was derived from fixation whereas the plants inoculated only with Bradyrhizobium derived 68% of their nitrogen from fixation and the control plants, 64%. Amount of P in plant increased significantly only by dual inoculation with VAM fungi and Bradyrhizobium.

In the sterilized soil a highly significant increase in the ARA was observed of the dual inoculation at all the sampling times. Nitrogen fixation determined by the A-value technique and N and P contents in plant also increased significantly by dual inoculation. Results obtained by the A-value method showed that plants with dual inoculation derived 68% of their nitrogen from fixation while the plants inoculated only with Bradyrhizobium, 38%.

From our this study we conclude that nitrogen fixation as well as N and P contents in peanut increased significantly only by dual inoculation with VAM fungi and Bradyrhizobium.  相似文献   

American Cocker Spaniel Chronic Hepatitis in Japan     

H. Kanemoto  M. Sakai  Y. Sakamoto  B. Spee  T.S.G.A.M. van den Ingh  B.A. Schotanus  K. Ohno  J. Rothuizen 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(5):1041-1048

  相似文献   

Automated analysis of fine‐root dynamics using a series of digital images     

Aiko Nakano  Hidetoshi Ikeno  Toshifumi Kimura  Hiromichi Sakamoto  Masako Dannoura  Yasuhiro Hirano  Naoki Makita  Leena Finér  Mizue Ohashi 《植物养料与土壤学杂志》2012,175(5):775-783

Information related to the growth of fine roots is important for understanding C allocation in trees and the mechanisms of C cycling in ecosystems. Observations using a camera or scanner embedded in the soil enabled us to obtain continuous images of fine‐root‐growth dynamics. However, these methods are still labor‐intensive because the image analysis has to be conducted manually. We developed an automated method for tracking movement or elongation of fine roots using a sequence of scanner images. We also show how data obtained with these methods can be used for calculating fine‐root behavior. Two A4‐size scanners were buried in a mixed forest in Japan and images were taken continuously from within the soil. We preprocessed these images by extracting the fine‐root area from the images and developed an automated calculation plug‐in we named A‐root for tracking growth movement of the tips of fine roots. A‐root and manual‐tracking results were compared using the same images. The results show the A‐root and manual‐tracking methods yielded similar levels of accuracy. The average growth rate of 17 fine roots tracked using the program was 0.16 mm h^–1. The observation of the direction of growth in fine roots showed the direction may be influenced by the original root's growth where the fine roots branched, distribution of soil particles, other roots, and the force of gravity. The A‐root analysis also suggested there may be an interaction between speed of growth and changes in direction of growing fine roots.  相似文献   

Control of Colletotrichum fructicola on strawberry with a foliar spray of neutral electrolyzed water through an overhead irrigation system     

Yoshihiko Hirayama  Shunsuke Asano  Keiichiroh Watanabe  Yasuhiro Sakamoto  Masaaki Ozaki  Ken’o Okayama  Satoshi T. Ohki  Motoaki Tojo 《Journal of General Plant Pathology》2016,82(4):186-189

  相似文献   

Changes in micropores in dry wood with elapsed time in the environment     

Keisuke Kojiro  Yuzo Furuta  Makoto Ohkoshi  Yutaka Ishimaru  Misao Yokoyama  Junji Sugiyama  Shuichi Kawai  Takumi Mitsutani  Hiromasa Ozaki  Minoru Sakamoto  Mineo Imamura 《Journal of Wood Science》2008,54(6):515-519

To investigate the changes in microstructures of wood with elapsed time in the environment, CO₂ adsorption onto dry wood was measured at ice-water temperature (273 K) for samples aged from 0.1 years to over 1000 years. The micropore size distribution was obtained using the Horvath-Kawazoe method. Micropores smaller than 0.6 nm in wood decreased in number with elapsed time in the environment, and a negative correlation was found between cumulative pore volume for pores smaller than 0.6 nm and elapsed time in the environment. Cumulative pore volume in the 1000-year sample was almost half of that in the 0.1- year sample. Micropores smaller than 0.6 nm in wood with a few decades or more of elapsed time increased in number after rewetting and drying. Consequently, microstructures of wood with longer time elapsed in the environment were considered to be more stable, because of longer-term thermal motion and possibly more repeated moisture adsorption and desorption and/or temperature variation in the environment.  相似文献   

Contrast‐enhanced ultrasonographic findings of hepatic arterioportal fistulas in a dog          下载免费PDF全文

M. Sakamoto  S. Shibata  R. Asahina  K. Yamazoe  H. Kamishina  K. Ishigaki  K. Asano  S. Maeda 《The Journal of small animal practice》2017,58(7):419-419

  相似文献