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61.
The aim of the present study was to evaluate the potential effects of olive leaf (Olea europea L.) extract (OLE) on common carp (Cyprinus carpio) health in response to ambient ammonia toxicity. Fish were fed with experimental diets containing OLE (0, 1, 5 and 10 g/kg) for 60 days followed by a 3‐hr challenge with ammonia (0.5 mg/L unionized ammonia‐N). Results showed that haemoglobin levels, differential leucocyte counts and mean corpuscular haemoglobin concentration were not affected by dietary OLE levels and ammonia challenge. Ammonia challenge significantly increased red blood cells and decreased blood mean corpuscular volume, mean corpuscular haemoglobin, plasma lysozyme, complement and bactericidal activities. Plasma lysozyme, complement and total immunoglobulin values improved by 5 and/or 10 g OLE/kg supplementation. Before the ammonia exposure, the 5 and 10 g OLE/kg treatment significantly increased blood white blood cell, but there was no significant difference among the treatments after the ammonia exposure. Ammonia exposure significantly increased plasma malondialdehyde levels; however, 1 and 5 g OLE/kg treatments had significantly lower values compared to the other treatments. The present results suggest that 1 g OLE/kg diet may improve fish health and reduce the adverse effects of ammonia toxicity on common carp.  相似文献   
62.
Common carp juveniles were fed two types of diet (control: 0.1% tryptophan and TRP: 0.6% tryptophan) over 15 days. Thereafter, both groups were directly subjected to osmotic challenge (from 0 to 10 ppt) for 168 h. Blood samples were collected at ?240, 0, 6, 24, 72 and 168 h after challenge. Survival and serum cortisol, glucose, sodium and chloride levels were measured to determine stress response and osmoregulation condition. While TRP group showed no mortality until 168 h, cumulative mortality was near 100% at 72 h after challenge in control group. Feeding tryptophan-supplemented diet led to increase in prechallenge cortisol, but not glucose, sodium and chloride values at ?240 h that stayed elevated until 0 h after challenge. After challenge, cortisol and glucose values did not significantly affected by time of sampling but type of diet and diet × time interaction. Sodium values significantly affected by diet type and time of sampling but not their interaction. Chloride values significantly affected by time of sampling but not diet type and their interaction. Control group had higher cortisol, glucose and sodium than TRP at each time of sampling. Control and TRP group showed increasing and decreasing pattern in cortisol and glucose values when experiment progressed after osmotic challenge. Both group showed increasing pattern in sodium and chloride values when experiment progressed after osmotic challenge. Results indicated that tryptophan supplementation enhanced salt water tolerance of carp that is due to increase in basal cortisol and anti-stress effect of tryptophan and possibly increase in serotonergic activity.  相似文献   
63.
Abstract

A two-year field experiment was conducted to study PGP bacteria on growth and yield productivity of sesame. Factors were control, Nitroxin, Biophosphor, and an equal combination of biofertilizers along with sesame capsule type. 1000-seed weight and oil content increased in the second year, but, seed yield reduced (-12.6?g m2), which represented a negative relationship between seed weight and yield. The effect of year on No. of single and multi-cap node plant?1 was reversed. Seed yield, chlorophyll, protein, and N increased in the multi-cap seed, whereas, 1000-seed weight and seed weight plant?1 was decreased. Maximum yield and yield components were achieved in the second and first year, respectively, along with the multi-cap seed. Nitroxin, after the equal combination, was approximately showed the maximum enhancement of growth and yield productivity of sesame. The most seed yield (4261 k ha?1) was performed in the first year, combined biofertilizers and multi-cap seed, whereas, the highest oil content was gained in the second year and single-cap that it was due to the higher N and P use efficiency in the first year. No. of node plant?1 and capsule No. plant?1 showed the most correlation with the other parameters. Seed yield was correlated with No. of node plant?1 (r?=?0.925) and leaf dry weight (r?=?0.885). The N and P use efficiency had been higher in the first year, multi-cap, and equal combination. Nitrogen Use efficiency was higher compared to phosphorous. The uptake of N and P by sesame increased with the integrated application of different bacteria.  相似文献   
64.
Gesunde Pflanzen - Management of plant nutrition using fertilizers is an approach to deal with drought and to increase water productivity in paddies. The aim of this study, therefore, was to...  相似文献   
65.
Cats are important in the epidemiology of Toxoplasma gondii because they are the only hosts that can excrete environmentally resistant oocysts. T.gondii is a major zoonotic agent which infects up to one-third of the world population. Toxoplasmosis in neonates and immunocompromised patients can lead to severe disease and death. A cross- sectional parasitological and serological survey with latex agglutination test (LAT) to detect anti-T. gondii antibodies was conducted on 100 serum samples collected from stray cats in five urban areas of Sari, Northern Iran, from April to November 2004. Classification by age, sex, weight, season and region was made. Results analyzed according to specific variables. The overall prevalence of T. gondii IgG antibodies (LAT titre ≥1:1) were found in 40 of 100(40%) of stray cats, with regional variations. Overall 16 of 100(16%) of stray cats had diagnostically significant antibody titres (LAT ≥ 1:64). Prevalence was significantly higher in adult cats (1.5–3.0 kg, 54.5% of 66) than in juvenile cats and kittens (≤1.4 kg, 11.8% of 34) and higher in female stray cats (44.4% of 72) than in male stray cats (28.6% of 28). Toxoplasma seroprevalence was highest in the season of spring (22.4%). There was a significant difference in the prevalence of infection relative to host age and weight (P < 0.05). No significant difference was found between the prevalence of infection relative to host gender, urban sites and season (P > 0.05). Prevalence of T. gondii oocyst was also analyzed by a routine coprological method in 100 cats. T. gondii oocysts were not found in any faecal samples analyzed. Only 2 out of 100 smear preparations of intestinal mucosa showed trophozoites of T. gondii.  相似文献   
66.
67.
Tropical Animal Health and Production - The present experiment was conducted to investigate the effect of using an extract of Ascophyllum nodosum algae (Tasco®, AN) on feed digestibility and...  相似文献   
68.
To understand epidemiology of Bacillus anthracis in Iran, the morphological, biochemical, and virulence specifications of 32 B. anthracis isolates, collected from human, sheep, cattle, goat, and environmental specimens obtained from throughout Iran were examined by conventional and molecular approaches. B. anthracis isolates were characterized in multiple ways: (1) capsule formation both on bicarbonate agar and in defibrinated horse blood, (2) motility of vegetative forms, (3) hemolysis on 5% sheep blood agar, (4) penicillin G susceptibility, (5) lecithinase production on egg yolk agar, (6) gelatin hydrolysis, (7) ability to develop “string of pearls” on tryptose agar, and (8) capability to develop mucoid colonies in presence of CO2 were assessed. In addition, biochemical properties such as indole, methyl red, catalase, citrate utilization, and finally nitrate reduction tests were used. All the tested isolates produced identical morphological and biochemical patterns with those of the vaccine strain B. anthracis 34F2 Sterne. In order to assess potential virulence of isolates at genomic level, PCR protocols assaying for the pXO1 and pXO2 loci were employed. The intriguing high level of phenotypic similarity between Iranian isolates of B. anthracis and the 34F2 Sterne strain deserves further studies at genomic level.  相似文献   
69.
Based on the antioxidant properties of essential oils, the effect of thyme, coriander, and rosemary was evaluated on the reduction of peroxidase in white cabbage “Brassica oleraceae var.capitata f. alba” and red cabbage “B. oleraceae var. capitata f. rubra”. To do this, pure, 50, 75, 100 and 200 μl/100 ml concentrations of essential oils were applied in vivo and in vitro of cabbages. Antioxidant activity of the essential oils was compared with that of ascorbic acid. Results revealed that the highest percentage of antioxidant activity was achieved in vitro using a pure concentration of coriander (52.65%) and rosemary (54.64%), and in vivo applying thyme (60.07%) of white cabbage. Applying in vitro pure concentration of coriander (46.39%) and 50 μl/100 ml of rosemary (41.64%), and in vivo 75 μl/100 ml of thyme (42.64%) in red cabbage showed high antioxidant activity. A high reduction of peroxidase was obtained in vitro using of essential oils in red and white cabbages.  相似文献   
70.
The present study aimed at assessing the effects of addition of thymol [0 (T0), 2.5 mg/L (T2.5), 5 mg/L (T5) and 10 mg/L (T10)] to water on biochemical and antioxidant responses of common carp, Cyprinus carpio, during 3-h transportation. The lowest dissolved oxygen and ammonia levels after transportation were related to T2.5 and T5 treatments respectively. T5 treatment exhibited lower cortisol/glucose responses after transportation and 24 h after that, whereas T2.5 and T10 exhibited elevations in these parameters at the same times. Hyperammonaemia and hyperchloraemia were observed in T2.5 treatment immediately after transportation, but such changes were observed in all treatments, 24 h after transportation. Addition of 10 mg/L thymol to water prevented uraemia after transportation, but all thymol-treated fish exhibited uraemia, 24 h after transportation. Thymol treatment failed to mitigate lactate elevation immediately after transportation; nevertheless, T2.5 and T10 had, respectively, the lowest and highest lactate levels, 24 h after transportation. T5 treatment inhibited alteration in superoxide dismutase activity, immediately after transportation, and T10 exhibited a similar effect, 24 h after transportation. Addition of 2.5 mg/L thymol to water significantly increased catalase activity immediately after transportation, but such a response was observed in all treatments, 24 h after transportation. T2.5 and T10 exhibited higher malondialdehyde levels than T0 immediately after transportation, but such a response was observed in all thymol-treated fish, 24 h after transportation. In conclusion, addition of 5 mg/L thymol is beneficial in reducing water ammonia concentration and stress in carp during transportation.  相似文献   
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