Detection and identification of the blackeye cowpea mosaic strain of <Emphasis Type="Italic">Bean common mosaic virus</Emphasis> in seeds of cowpea from southern India     

Arakere C. Udaya Shankar  Chandra S. Nayaka  Bhuvanendra H. Kumar  Shekar H. Shetty  Harischandra S. Prakash 《Phytoparasitica》2009,37(3):283-293

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Genetic and non-genetic factors affecting semen production traits in Karan Fries crossbred bulls     

Sen Chauhan  Indra  Gupta  Ashok K.  Khate  Keviletsu  Chauhan  Anuj  Krishna Shankar Rao  Thakur  Pathak  Shivendra  Hazra  Ritwik  Singh  Maneesh 《Tropical animal health and production》2010,42(8):1809-1815

The objective of the study was to evaluate the effects of genetic and non-genetic factors on production of breeding bulls and semen quality parameters in Karan Fries crossbred male by fitting least squares analysis. Genetically, the animals were divided into three subclasses. The non-genetic factors were season of birth, period of birth, and age group with three subclasses each for season of birth and period of birth. Age group was classified into four subclasses. The traits generated in the study were number of males reaching semen donation stage (AFSC) and first freezing (AFSF), age at last semen collection (ALSC) and last freezing (ALSF), age at disposal (AD), and lifetime semen production traits (up to 1 year after first freezing). The effect of period of birth was significant for AFSC, AFSF, ALSC, and AD. It was also significant for total ejaculates produced in a year. The age group had significant effect on AFSF. Effect of genetic group was significant for freezable ejaculates produced in a year, for frozen semen doses produced in a year, and for number of ejaculates cryoprocessed in a year. Season had no statistically significant effect on any of the traits studied. The influence of period revealed that the most of the traits of breeding bulls improved after intermediate period, which could be due to better care, training, feeding, and other management practices in the latter years. However, no consistent trend could be established for the effects of genetic groups and other non-genetic causes on the traits considered.  相似文献   

Development of biofilm of <Emphasis Type="Italic">Vibrio alginolyticus</Emphasis> for oral immunostimulation of shrimp     

S. R. Krupesha Sharma  K. M. Shankar  M. L. Sathyanarayana  Raj Reddy Patil  H. D. Narayana Swamy  Suguna Rao 《Aquaculture International》2011,19(3):421-430

Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation was studied at various nutrient concentrations and incubation time. Highest colony-forming units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of incubation. The BF cells could be completely inactivated at 80°C in 10 min and with 10% formalin in 24 h. SDS–PAGE profile of BF cells revealed repression of four proteins and expression of three new proteins compared to free cells (FC). The preliminary immune response studies showed that BF cells were superior to FC in stimulating the non-specific immune response of Penaeus monodon.  相似文献   

Contribution of Post-Anthesis Growth and Manganese Dynamics to Differential Grain Yield in Different Wheat Species     

Arun Shankar  Upkar S. Sadana  Nirmal K. Sekhon 《Journal of plant nutrition》2014,37(11):1770-1781

Manganese (Mn) deficiency has become a serious nutritional problem for wheat grown in alkaline coarse textured soil. The study aimed to investigate post-anthesis Mn partitioning in different wheat species. Cultivars of bread wheat (‘PBW509’, ‘DBW17’, ‘PBW550’ and ‘PBW636’); durum wheat (‘PDW291’) and triticale (‘TL2908’) were grown in 6.5 L pots with two treatments of Mn (0 and 50 mg Mn kg^?1 soil) in screen house and harvested at anthesis, 18- days post-anthesis, and maturity to record Mn uptake. Durum cv. ‘PDW291’ retained highest proportion of Mn in its vegetative parts under Mn deficiency resulting into lowest partitioning to the grain and had the lowest grain yield. All bread wheat cv. facilitated superior Mn partitioning to the grain, lesser retention in vegetative organs and higher Mn utilization efficiency, than triticale and durum wheat species. Cultivars producing higher yield on Mn deficit soils are viable alternative to foliar application of Mn.  相似文献   

Molecular Characterization of Oxytocin Receptor Gene in Water Buffalo (Bubalus bubalis)     

N Arunmozhi  SK Singh  T Sarath  SK Agarwal  A Doiphode  U Shankar 《Reproduction in domestic animals》2014,49(5):E56-E59

Buffaloes are known for their productivity as compared to average yielding cows due to higher fat percentage, better feed conversion ability and disease resistance. On the other hand, the reproductive performances of buffaloes are often considered as poor owing to late sexual maturity, weak/silent oestrus, repeat breeder and prolonged intercalving interval. The study of cascade of events during oestrus and oestrous cycle can be useful for the improvement of reproductive efficiency of buffaloes. More precisely, the hormonal changes initiated at the molecular level within the animal determine the reproductive nature of the species. Nucleotide/protein sequence analysis serves as a vital tool in analysing the binding of the hormones for their effect or functions. In this study, we have reported cloning and characterization of the complete coding (cDNA) sequence of oxytocin receptor gene (OXTR) in buffaloes. Buffalo OXTR gene contains an uninterrupted ORF of 1176 nucleotides corresponding to an inferred polypeptide length of 391 amino acids (aa). The molecular weight of the deduced aa sequence was found to be 43 kDa with an isoelectric point of 9.253 and 16.328 charge at pH 7.0. The deduced protein sequence consists of 38 strongly basic (+) (K,R), 22 strongly acidic (?) (D,E), 186 hydrophobic (A, I, L, F, W, V) and 95 Polar (N, C, Q, S, T, Y) aa. Results indicated that aspartate (D) at aa position 85 and D, R and C at aa positions 136, 137 and 138, respectively, are conserved in buffaloes. The buffalo OXTR gene shared a per cent similarity ranging from 84.7 to 98.1 and 88.5 to 97.7 at nucleotide and deduced aa sequence levels, respectively, with that of other species. Phylogram constructed on the basis of either nucleotide or deduced aa sequences of buffalo OXTR gene showed that buffalo, cattle and sheep have diverged from human and swine and formed a separate clad. The buffalo sequence has shown maximum similarity and closeness with cattle followed by sheep both at nucleotide and at aa level.  相似文献   

Chemical composition and antioxidant property of holy basil (Ocimum sanctum L.) leaves, stems, and inflorescence and their in vitro callus cultures     

Hakkim FL  Shankar CG  Girija S 《Journal of agricultural and food chemistry》2007,55(22):9109-9117

In this study, the chemical constituents and antioxidant property of holy basil (Ocimum sanctum Linn.) field-grown plant parts (leaves, stems, and inflorescence) were compared with those of respective callus cultures induced from each explant in in vitro. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1 mg/L) combined with different concentrations (0.1-0.5 mg/L) of kinetin as plant growth regulators. The distribution of phenolic compounds in these extracts was analyzed using reverse phase high-performance liquid chromatography with reference standards. Interestingly, rosmarinic acid (RA) was found to be the predominant phenolic acid in all callus extracts in comparison with field-grown plant parts. In this study, the antioxidant activity of the extracts was evaluated with six different in vitro antioxidant-testing systems. Their activities of scavenging superoxide anion radicals, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), hydroxyl radicals, hydrogen peroxide, chelating ferrous iron, and ferric ion reducing potential were assessed. The antioxidant activity was increased in all testing systems with increasing amounts of extract. However, at the same concentration, the callus extracts exhibited higher antioxidant activity in all of the testing systems than the extract obtained from field-grown plant parts. The data obtained from this study suggested the possibility of the isolation of a high content of RA from in vitro callus cultures rather than field-grown plant organs of holy basil.  相似文献   

Crop water stress index for scheduling irrigation of Indian mustard (Brassica juncea) based on water use efficiency considerations     

Navsal Kumar  Arunava Poddar  Vijay Shankar  Chandra Shekhar Prasad Ojha  Adebayo Johnson Adeloye 《Journal of Agronomy and Crop Science》2020,206(1):148-159

The study was conducted to determine the feasibility of canopy temperature based crop water stress index (CWSI) for scheduling irrigation of Indian mustard (Brassica juncea). Field crop experiments were conducted in Hamirpur, Himachal Pradesh (India) during three consecutive cropping seasons (2015, 2016 and 2017). The experimental field was divided into five plots with different levels of irrigation treatments based on depletion of total available soil water (TASW) in the crop root zone. The maximum soil moisture depletion (SMD) of TASW at 10%, 30% and 50%, full irrigated (non-stressed) and extremely dry (full stressed) conditions were maintained in respective plots. Relationships were developed between canopy-air temperature differential (T_C-T_A) and vapour pressure deficit (VPD) for non-stressed and fully stressed conditions to generate non-water-stressed baseline (NWSB) and maximum water-stressed baseline (MWSB) baselines for Indian mustard crop. The CWSI was computed for different SMD of TASW by using a proven empirical approach based on the baselines. The irrigation treatment corresponding to 30% SMD with a mean CWSI of 0.4 resulted in optimal yield and maximum water use efficiency. Results of the study suggest that established CWSI value can be used to detect stress and schedule irrigations for Indian mustard.  相似文献   

Quantitative trait loci for seedling and adult plant resistance to Stagonospora nodorum in wheat     

Shankar M  Walker E  Golzar H  Loughman R  Wilson RE  Francki MG 《Phytopathology》2008,98(8):886-893

Stagonospora nodorum blotch (SNB) caused by Stagonospora nodorum is a severe disease of wheat (Triticum aestivum) in many areas of the world. S. nodorum affects both seedling and adult plants causing necrosis of leaf and glume tissue, inhibiting photosynthetic capabilities, and reducing grain yield. The aims of this study were to evaluate disease response of 280 doubled haploid (DH) individuals derived from a cross between resistant (6HRWSN125) and susceptible (WAWHT2074) genotypes, compare quantitative trait loci (QTL) for seedling and adult plant resistance in two consecutive years, and assess the contribution of QTL on grain weight. Flag leaves and glumes of individuals from the DH population were inoculated with mixed isolates of S. nodorum at similar maturity time to provide accurate disease evaluation independent of morphological traits and identify true resistance for QTL analysis. Fungicide protected and inoculated plots were used to measure relative grain weight (RGW) as a yield-related trait under pathogen infection. The lack of similar QTL and little or no correlation in disease scores indicate different genes control seedling and adult plant disease and independent genes control flag leaf and glume resistance. This study consistently identified a QTL on chromosome 2DL for flag leaf resistance (QSnl.daw-2D) and 4BL for glume resistance (QSng.daw-4B) from the resistant parent, 6HRWSN125, explaining 4 to 19% of the phenotypic variation at each locus. A total of 5 QTL for RGW were consistently detected, where two were in the same marker interval for QSnl.daw-2D and QSng.daw-4B indicating the contribution of these QTL to yield related traits. Therefore, RGW measurement in QTL analysis could be used as a reliable indicator of grain yield affected by S. nodorum infection.  相似文献   

Brucellosis among smallholder cattle farmers in Zambia     

Muma JB  Pandey GS  Munyeme M  Mumba C  Mkandawire E  Chimana HM 《Tropical animal health and production》2012,44(4):915-920

A cross-sectional study was performed in Southern and Lusaka provinces of Zambia between March and September 2008 to estimate Brucella seroprevalence in cattle kept by smallholder dairy farmers (n = 185). Rose Bengal test (RBT) was used as a screening test followed by confirmation with competitive ELISA (c-ELISA). We investigated 1,323 cattle, of which 383 had a history of receiving vaccination against brucellosis and 36 had a history of abortion. Overall seroprevalence was 6.0% with areas where vaccination was practiced having low seroprevalence. Age was associated with Brucella seropositivity (P = 0.03) unlike cattle breed (P = 0.21) and sex (P = 0.32). At area level, there was a negative correlation (Corr. coeff = −0.74) between percentage of animals with brucellosis vaccination history (vaccination coverage) and level of brucellosis; percentage of animals with history of abortion (Corr. coeff. = −0.82) and brucellosis vaccination coverage. However, a positive correlation existed between brucellosis infection levels with percentage of animals having a history of abortion (Corr. coeff. = 0.72). History of vaccination against brucellosis was positively associated with a positive Brucella result on RBT (P = 0.004) whereby animals with history of vaccination against brucellosis were more likely to give a positive RBT test results (OR = 1.52). However, the results of c-ELISA were independent of history of Brucella vaccination (P = 0.149) but was positively associated with history of abortion (OR = 4.12). Our results indicate a relatively low Brucella seroprevalence in cattle from smallholder dairy farmers and that vaccination was effective in reducing cases of Brucella infections and Brucella-related abortions. Human exposure to Brucella through milk from smallholder farmers could result through milk traded on the informal market since that milk is not processed and there no quality and safety controls.  相似文献   

Influence of foliar application of mineral nutrients at different growth stages of guava     

Jitendra Kumar  Rajesh Kumar  Ratna Rai  Daya Shankar Mishra  Saurabh Kumar Singh  Prashant Kisan Nimbolkar 《Journal of plant nutrition》2017,40(5):656-661

Foliar application of nutrients enhances efficient use along with quick response. The experiment was undertaken to study the effect of foliar application of mineral nutrients viz., boron (B), zinc (Zn), calcium (Ca), and potassium (K) sprayed at different growth stages viz., at fruit set and at two weeks after fruit set of guava (Psidium guajava L.). Increase in plant canopy and minimum seed index was in Zn (0.03%) sprayed at fruit set. Fruit size, sugar: acid, and organoleptic score were better in K (0.5%) sprayed at two weeks after fruit set. Increased yield in Zn (0.01%) and pectin enhancement with B (0.03%) was found at two weeks after fruit set, respectively. Minimum physiological loss in weight of fruits was with Ca (0.5%) sprayed at two weeks after fruit set. In general, for most of the desirable characters 0.5% potassium (K) spray at 2 weeks after fruit set had better results.  相似文献