Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field     

B Adil  K. M. Shankar  B. T. Naveen Kumar  Rajreddy Patil  Abhiman Ballyaya  K. S. Ramesh  Sathish Rama Poojary  Omkar V. Byadgi  Prabhugouda Siriyappagouder 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):413-419

A monoclonal antibody-based flow-through immunoassay (FTA) was developed using a nitrocellulose membrane placed on the top of adsorbent pads enclosed in a plastic cassette with a test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.) invadans. The FTA limit of detection was 7 µg/mL for A. invadans compared to 56 µg/mL for the immunodot. FTA and polymerase chain reaction (PCR) could detect A. invadans in fish tissue homogenates at a 10^-11 dilution compared to a 10^-8 dilution by immunodot. In fish suffering from natural cases of epizootic ulcerative syndrome (EUS) collected from Mangalore, India, FTA and PCR could detect A. invadans in 100% of the samples compared to 89.04% detected by immunodot. FTA reagents were stable and produced expected results for 4 months when stored at 4~8℃. This rapid test could serve as simple and cost-effective on-site screening tool to detect A. invadans in fish from EUS outbreak areas and in ports during the shipment of live or frozen fish.  相似文献   

Molecular characterization of a poxvirus isolated from an American flamingo (Phoeniconais ruber rubber)     

Mondal SP  Lucio-Martínez B  Buckles EL 《Avian diseases》2008,52(3):520-525

An avian poxvirus from the beak scab of an American flamingo (Phoeniconais ruber rubber) was isolated by inoculation on the chorioallantoic membrane (CAM) of specific-pathogen-free (SPF) chicken embryos. The virus produced multifocal areas of epithelial hyperplasia along with foci of inflammation in the CAM, and rare cells contained small eosinophilic intracytoplasmic bodies. Chickens inoculated with the isolated virus in the feather follicle of the leg did not develop significant lesions. Nucleotide sequence comparison of a PCR-amplified 4.5 kb HindIII fragment of the genome of flamingo poxvirus (FlPV) revealed very high homology (99.7%) with condor poxvirus (CPV), followed by approximately 92% similarity with canary poxvirus (CNPV) and Hawaiian goose poxvirus (HGPV), but less similarity (approximately 69%) to fowl poxvirus (FPV), the type species of the genus Avipoxvirus of family Poxviridae. As in the cases with CPV, CNPV, and HGPV, genetic analysis of FlPV revealed an absence of three corresponding FPV open reading frames (ORF199, 200, and 202) and an absence of any reticuloendotheliosis virus (REV) sequences in this region. There are only nine nucleotide substitutions observed between FlPV and CPV in the 4.5 kb fragment; those were clustered in the ORF201 region, which in FPV genome is a site for integration of REV sequences. Phylogenetic analysis of the predicted amino acid sequences of the ORF201-coded hypothetical protein demonstrated FlPV to be more closely related to CPV, as well as to CNPV and HGPV, than to FPV.  相似文献   

Trends in Mango Research as seen Through Science Citation Expanded Index of Web of Science     

Shankar Reddy Kolle  T. H. Shankarappa  T. B. Manjunatha Reddy 《Erwerbs-Obstbau》2018,60(3):261-270

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Establishing indicator leaf and its threshold values for need based nitrogen management using chlorophyll meter and leaf color chart in Bt cotton     

Arun Shankar  Bijay Singh 《Journal of plant nutrition》2019,42(2):186-201

Over application of fertilizer N to cotton is not only a potential threat to environment but also leads to increased costs of cultivation. The study aimed to establish the indicator leaf and its critical greenness for in-season management of fertilizer nitrogen (N) in Bt cotton using chlorophyll (SPAD) meter and leaf color chart (LCC). The response of three varieties and N treatments viz. 0, 30, 60, 90,120, 150 and 180?kg N ha^?1 applied in two splits {(50% at thinning and 50% at first flowering) and three splits (50% at thinning, 25% at first flowering and 25% at boll formation)} was studied through split plot design. SPAD values and LCC scores of first, second, third and fourth fully opened leaves from the top of the main stem was recorded at first flowering and boll formation. The physiological efficiency and harvest index was highest for 90?Kg N ha^?1 applied in two splits. Beyond 120?kg N ha^?1, the N use efficiency parameters were higher for the N treatments applied in three split compared to the respective two split N treatments. The fourth leaf from the top in terms of SPAD values and LCC scores correlated best with N concentration compared to other leaves at all growth stages. The calculated critical SPAD values for the fourth leaf were 45 and 41 at first flowering and boll formation, respectively. Critical score of fourth leaf was 4.1 and 4, respectively at first flowering and boll formation, respectively. It is suggested that color of the fourth leaf from the top of Bt cotton can well indicate N supply from the soil and can help in need based N management.  相似文献   

Long-term conservation agriculture and intensified cropping systems: Effects on growth, yield, water, and energy-use efficiency of maize in northwestern India     

Chiter M. PARIHAR  Malu R. YADAV  Shankar L. JAT  Aditya K. SINGH  Bhupender KUMAR  Vijay POONIYA  Sanatan PRADHAN  Rakesh K. VERMA  Mangi L. JAT  Raj K. JAT  Muli D. PARIHAR  Hari S. NAYAK  Yashpal S. SAHARAWAT 《土壤圈》2018,28(6):952-963

Conservation agriculture(CA)-based best-bet crop management practices may increase crop and water productivity, while conserving and sustaining natural resources. We evaluated the performance of rainy season maize during 2014 under an ongoing long-term trial(established in 2008) with three tillage practices, i.e., permanent bed(PB), zero tillage(ZT), and conventional tillage(CT) as main plots, and four intensified maize-based cropping systems, i.e., maize-wheat-mungbean, maize-chickpea-Sesbania(MCS), maizemustard-mungbean, and maize-maize-Sesbania) as subplot treatments. In the seventh rainy season of the experiment, maize growth parameters, yield attributes, yield, and water-and energy-use efficiency were highest at fixed plots under ZT. Maize growth parameters were significantly(P 0.05) superior under ZT and PB compared with CT. Maize yield attributes, including cobs per m~2(7.8), cob length(0.183 m), grain rows per cob(13.8), and grains per row(35.6), were significantly higher under ZT than CT; however, no significant effect of cropping systems was found on maize growth and yield attributes. Zero tillage exhibited the highest maize productivity(4 589 kg ha~(-1)). However, among the cropping systems, MCS exhibited the highest maize productivity(4 582 kg ha~(-1)). In maize, water use was reduced by 80.2–120.9 mm ha-1under ZT and PB compared with CT, which ultimately enhanced the economic water-use efficiency by 42.0% and 36.6%, respectively. The ZT and PB showed a 3.5%–31.8% increase in soil organic carbon(SOC) at different soil depths(0–0.45 m), and a 32.3%–39.9% increase in energy productivity compared with CT. Overall, our results showed that CA-based ZT and PB practices coupled with diversified maize-based cropping systems effectively enhanced maize yield and SOC,as well as water-and energy-use efficiency, in northwestern India.  相似文献   

Limited evidence for genetic variation for resistance to the white spot syndrome virus in Indian populations of Penaeus monodon     

Ben J Hayes  Thomas Gitterle  Gopalapillay Gopikrishna  Chavali Gopal  Gopal Krishna  Shrivinas Jahageerdar  Carlos Lozano  Shankar Alavandi  Sivagnanam Paulpandi  Pitchaiyappan Ravichandran  Morten Rye 《Aquaculture Research》2010,41(11):e872-e877

There has been a highly detrimental impact of the white spot syndrome virus (WSSV) on black tiger shrimp (Penaeus monodon) aquaculture in India. Currently, no cost‐effective measures are available for controlling the disease. One alternative is to improve WSSV resistance through a selective breeding programme for disease‐resistant shrimp, provided that genetic variation exists for this trait. The aim of this study was to evaluate the evidence for genetic variation in resistance to WSSV in P. monodon sourced from Indian populations. Post‐larval shrimp (n=1950) from 54 full‐sibling families were challenged with WSSV using WSSV‐infected mince meat. The heritability was estimated using four different statistical models fitted to the resulting time to death data, including two linear models and two Weibull proportional hazard frailty models. None of the estimated heritabilities were significantly different from zero. We suggest three possible explanations for these results: there actually is very little variation between P. monodon in WSSV resistance and all individuals are highly susceptible to the disease; there is genetic variation in resistance to WSSV in P. monodon but we did not find it in our experiment because the level of challenge in the experiment was too high to allow genetic differences to be expressed; the variation is due to mutations conferring resistance, which are at a low frequency in the population, and we did not sample a broad enough genetic base to capture these mutations.  相似文献   

Publication Trends in Food-Borne Disease Research (1991–2015): A Web of Science Core Collection Based Analysis     

Shankar Reddy Kolle  T. H. Shankarappa 《Journal Of Agricultural & Food Information》2017,18(1):53-63

This article analyzes publication trends in food-borne disease research for the period 1991–2015, using data collected from the Web of Science Core Collection. A total of 11,725 research articles were used in the analysis. Results showed that the number of publications rose exponentially during the period of study. The average number of authors per article also increased. “Food Science & Technology” was the leading subject category under which the majority of articles placed. The Journal of Food Protection was the most productive journal with 794 articles. “Kang, DH” was the most productive author and the United States was the most productive country, followed by China.  相似文献   

Scaling properties in landscape patterns: New Zealand experience   总被引：15，自引：0，他引：15  

Vladimir I. Nikora  Charles P. Pearson  Ude Shankar 《Landscape Ecology》1999,14(1):17-33

In this paper we present a case study of spatial structure in landscape patterns for the North and South Islands of New Zealand. The aim was to characterise quantitatively landscape heterogeneity and investigate its possible scaling properties. The study examines spatial heterogeneity, in particular patchiness, at a range of spatial scales, to help build understanding on the effects of landscape heterogeneity on water movement in particular, and landscape ecology in general.We used spatial information on various landscape properties (soils, hydrogeology, vegetation, topography) generated from the New Zealand Land Resource Inventory. To analyse this data set we applied various methods of fractal analyses following the hypothesis that patchiness in selected landscape properties demonstrates fractal scaling behaviour at two structural levels: (1) individual patches; and (2) mosaics (sets) of patches.Individual patches revealed scaling behaviour for both patch shape and boundary. We found self-affinity in patch shape with Hurst exponent H from 0.75 to 0.95. We also showed that patch boundaries in most cases were self-similar and in a few cases of large patches were self-affine. The degree of self-affinity was lower for finer patches. Similarly, when patch scale decreases the orientation of patches tends to be uniformly distributed, though patch orientation on average is clearly correlated with broad scale geological structures. These results reflect a tendency to isotropic behaviour of individual patches from broad to finer scales. Mosaics of patches also revealed fractal scaling in the total patch boundaries, patch centers of mass, and in patch area distribution. All these reflect a special organisation in patchiness represented in fractal patch clustering. General relationships which interconnect fractal scaling exponents were derived and tested. These relationships show how scaling properties of individual patches affect those for mosaics of patches and vice-versa. To explain similarity in scaling behaviour in patchiness of different types we suggest that the Self-Organised Criticality concept should be used. Also, potential applications of our results in landscape ecology are discussed, especially in relation to improved neutral landscape models.  相似文献   

Integration of substrate in biofloc based system: Effects on growth performance,water quality and immune responses in black tiger shrimp,Penaeus monodon culture     

Sujeet Kumar  Panantharayil Sudhayam Shyne Anand  Debasis De  Tapas Kumar Ghoshal  Sankar V. Alavandi  Koyadan Kizhakedath Vijayan 《Aquaculture Research》2019,50(10):2986-2999

To evaluate effect of substrate integration in biofloc based system, a 52‐day growth experiment was conducted using black tiger shrimp, Penaeus monodon juveniles (3.32 ± 0.07 g). The factorial design consisted of floc, F (with or without) as first factor and substrate (bamboo mat, B; nylon mesh, N; and without substrate) as second factor. This resulted six treatments; F + B, F + N, F, B, N and a control without biofloc and substrate. Shrimps were stocked at 110 nos. m^–3 in Fibre Reinforced Plastic (FRP) tanks and, rice flour was used as carbon source in biofloc based treatments. Incorporation of nylon mesh and bamboo mat in biofloc system trapped 31.3%–38.6% and 8.5%–13.5% total suspended solids respectively and reduced bottom solid deposition. Among the substrate based groups, significantly better development of biofilm with higher microbial population noticed in F + B compared with nylon mesh. Similarly, significantly higher final growth (p < 0.01) was recorded in F + B system followed by F + N while no significant difference in body weight recorded among floc, F or substrate based groups (B, N). Biofloc and substrate integration (F + B and F + N) resulted significantly (p < 0.01) lower feed conversion ratio compared to control and floc. Incoporation of bamboo substrate in biofloc, (F + B) improved shrimp immune responses through higher hemocyte counts and prophenoloxidase activity compared to other treatments. The study revealed that integration of substrate in the biofloc system improved growth performance, FCR and immune parameters in shrimp by trapping the suspended biofloc particles, better water quality parameters, enhanced biofilm growth and provision of quality natural food.  相似文献   

Management of Sesbania aculeata Incorporation and Nitrogen on the Performance of Transplanted Rice in Calcareous Soil     

Sanjay Kumar Chaudhary  Shankar Jha  Yogeshwar Singh 《Communications in Soil Science and Plant Analysis》2018,49(14):1739-1749

Two field experiments were conducted to optimize the days for decomposition of dhaincha (Sesbania aculeata) with different nitrogen (N) levels and scheduling in transplanted rice in calcareous soil in a split-plot design with three replications. Incorporation of dhaincha one day before transplanting (1-DBT) obviated the need for allowing N gap. Nitrogen scheduling as 50% at active tillering + 40% at panicle initiation + 10% at flowering recorded the maximum grain yield (59.05 q ha^?1) and N–?phosphorus (P)–?potassium (K) uptake. The different N fractions in post-harvest soil were in the order of total N> total hydrolyzable N> non-hydrolyzable N> exchangeable ammonium (NH₄⁺)–?N and nitrate (NO₃^?)–?N. Thus, in calcareous soil, rice may be transplanted immediately after burying the dhaincha without any time gap along with 80 kg N ha^?1. Also, application of nitrogenous fertilizer in three splits, delaying N application until active tillering stage, is beneficial for improving rice productivity.  相似文献