全文获取类型
| 收费全文 | 22482篇 |
| 免费 | 350篇 |
| 国内免费 | 467篇 |
专业分类
| 林业 | 4116篇 |
| 农学 | 1829篇 |
| 基础科学 | 410篇 |
| 3486篇 | |
| 综合类 | 3176篇 |
| 农作物 | 2495篇 |
| 水产渔业 | 2064篇 |
| 畜牧兽医 | 2066篇 |
| 园艺 | 1530篇 |
| 植物保护 | 2127篇 |
出版年
| 2024年 | 27篇 |
| 2023年 | 113篇 |
| 2022年 | 267篇 |
| 2021年 | 328篇 |
| 2020年 | 324篇 |
| 2019年 | 287篇 |
| 2018年 | 2948篇 |
| 2017年 | 3017篇 |
| 2016年 | 1449篇 |
| 2015年 | 448篇 |
| 2014年 | 389篇 |
| 2013年 | 436篇 |
| 2012年 | 1309篇 |
| 2011年 | 2627篇 |
| 2010年 | 2571篇 |
| 2009年 | 1675篇 |
| 2008年 | 1671篇 |
| 2007年 | 1899篇 |
| 2006年 | 263篇 |
| 2005年 | 291篇 |
| 2004年 | 206篇 |
| 2003年 | 235篇 |
| 2002年 | 141篇 |
| 2001年 | 42篇 |
| 2000年 | 78篇 |
| 1999年 | 37篇 |
| 1998年 | 13篇 |
| 1997年 | 12篇 |
| 1996年 | 17篇 |
| 1995年 | 15篇 |
| 1994年 | 11篇 |
| 1993年 | 21篇 |
| 1992年 | 21篇 |
| 1991年 | 9篇 |
| 1990年 | 14篇 |
| 1989年 | 10篇 |
| 1988年 | 17篇 |
| 1987年 | 4篇 |
| 1985年 | 6篇 |
| 1984年 | 4篇 |
| 1983年 | 3篇 |
| 1982年 | 5篇 |
| 1981年 | 5篇 |
| 1977年 | 6篇 |
| 1975年 | 5篇 |
| 1973年 | 2篇 |
| 1968年 | 4篇 |
| 1962年 | 3篇 |
| 1956年 | 2篇 |
| 1936年 | 3篇 |
排序方式： 共有10000条查询结果，搜索用时 15 毫秒
81.
To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention. 相似文献
82.
木结构建筑具有自然舒适、绿色环保、节能保温以及防震减灾等特点,在生态养老领域具有很好的发展前景。介绍了木结构建筑概况,分析了老年人的生态养老需求,从适老化、绿色智能和文旅康养等方面,对木结构老年建筑发展进行展望与讨论,提出了完善相关标准规范和政策建议,为促进我国木结构老年建筑发展提供参考。 相似文献
83.
Context
Playa wetlands are the primary habitat for numerous wetland-dependent species in the Southern Great Plains of North America. Plant and wildlife populations that inhabit these wetlands are reciprocally linked through the dispersal of individuals, propagules and ultimately genes among local populations.Objective
To develop and implement a framework using network models for conceptualizing, representing and analyzing potential biological flows among 48,981 spatially discrete playa wetlands in the Southern Great Plains.Methods
We examined changes in connectivity patterns and assessed the relative importance of wetlands to maintaining these patterns by targeting wetlands for removal based on network centrality metrics weighted by estimates of habitat quality and probability of inundation.Results
We identified several distinct, broad-scale sub networks and phase transitions among playa wetlands in the Southern Plains. In particular, for organisms that can disperse >2 km a dense and expansive wetland sub network emerges in the Southern High Plains. This network was characterized by localized, densely connected wetland clusters at link distances (h) >2 km but <5 km and was most sensitive to changes in wetland availability (p) and configuration when h = 4 km, and p = 0.2–0.4. It transitioned to a single, large connected wetland system at broader spatial scales even when the proportion of inundated wetland was relatively low (p = 0.2).Conclusions
Our findings suggest that redundancy in the potential for broad and fine-scale movements insulates this system from damage and facilitates system-wide connectivity among populations with different dispersal capacities.84.
Min Zhang Dan Zheng Zhimei Peng Yuting Zhu Rongrong Li Qiong Wu Yan Li Hongyi Li Weihua Xu Mao Zhang Qichun Hung Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131
In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers. 相似文献
85.
Context
Common indicators are needed to monitor biodiversity loss and the implications for the sustainable provision of ecosystem services (ES). A plethora of different sets of indicators may hinder the identification of major endpoints for large-scale assessments of biodiversity and ecosystem services (BES).Objectives
We aim to describe the main challenges of indicators for BES assessment and provide suggestions for establishing a comprehensive indicator system.Methods
An extensive literature review was conducted in this study. We review the main challenges of indicators for BES assessment and propose corresponding improvements from our perspectives of theory and practical applications.Results
The main theoretical challenges of existing indicators include inconsistent definitions and classifications of ES, misunderstanding of linkages among biodiversity, ecosystem structure, functions and services, and practical problems relate to such issues as indicator representativeness, data availability, and uncertainty. Our suggested improvements include clarifying the main terms, concepts and classification of indicators, establishing a robust conceptual framework with clear interactions among different components and indicators, selecting indicators based on ecosystem properties, streaming existing data into one platform, and strengthening validation of proxies. The steps for constructing a comprehensive indicator system for BES assessment are summarized.Conclusions
Clear definitions of key terms are indispensable to classify indicators and construct a conceptual framework. Improved understanding of the relations among indicators of biodiversity, ecosystem functions, and ES across multiple scales can guide the development of the indicator system. The integrated indicator system is an important tool for BES assessment to support decision making for sustainable development.86.
对香椿木材的物理力学性质及抽提物含量进行了系统研究。结果表明:香椿木材为轻质木材,气干密度0.45 g/cm3;气干材径向、弦向、体积干缩率分别为1.53%、3.21%、4.85%;全干材径向、弦向、体积干缩率分别为3.33%、6.56%、10.46%;饱水径向、弦向、体积湿胀率分别为3.45%、7.01%、11.67%;香椿木材顺纹抗压强度25.64 MPa,抗弯强度75.01 MPa,抗弯弹性模量6331.24 MPa,端面、径面、弦面的硬度分别为2.90 k N、1.91 k N、2.08 k N;香椿木材的热水抽提物含量为5%、苯醇抽提物含量为4.47%、1%No OH抽提物含量为20%。 相似文献
87.
Huimin Yang Murray Unkovich Ann McNeill Xianzhi Wang 《Biology and Fertility of Soils》2011,47(4):377-385
Symbiotic N2 fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil
mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in
an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18
permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N2 fixation using 15N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO3− by lucerne. On average 65% of lucerne N was obtained from symbiotic N2 fixation. Converting standing dry matter estimates to annual N2 fixation amounts we calculated average N2 fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year.
We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher
than that of non-N2 fixing species examined. 相似文献
88.
89.
MicroRNAs (miRNAs)are a class of non-coding, endogenous, single-stranded small RNA molecules composed of 19~25 nucleotides. miRNAs are widely involved in the process of human life activities. Recent studies have shown that part of miRNAs regulate the vascular endothelial function and angiogenesis. High expression of miRNA-21 is found to play important roles in the cell proliferation, cell apoptosis, cell growth and death of vascular endothelial cells. This review will focus on the recent progress related to miRNAs in vascular endothelial function and angiogenesis, providing a new insight in cardiovascular disease prevention, clinical diagnosis, prognosis and target therapeutics. 相似文献
90.
Andrea C. Varella David K. Weaver Jason P. Cook Nancy K. Blake Megan L. Hofland Peggy F. Lamb Luther E. Talbert 《Euphytica》2017,213(7):153
Plant landraces have long been recognized as potential gene pools for biotic and abiotic stress-related genes. This research used spring wheat landrace accessions to identify new sources of resistance to the wheat stem sawfly (WSS) (Cephus cinctus Norton), an important insect pest of wheat in the northern Great Plains of North America. Screening efforts targeted 1409 accessions from six geographical areas of the world where other species of grain sawflies are endemic or where a high frequency of accessions possesses the resistance characteristic of solid stems. Resistance was observed in approximately 14% of accessions. Half of the lines displayed both antixenosis and antibiosis types of resistance. Among the resistant accessions, 41% had solid or semi-solid stems. Molecular genetic screening for haplotypes at the solid stem QTL, Qss.msub.3BL, showed that 15% of lines shared the haplotype derived from ‘S-615’, the original donor of the solid stem trait to North American germplasm. Other haplotypes associated with solid stems were also observed. Haplotype diversity was greater in the center of origin of wheat. Evaluation of a representative set of resistant landrace accessions in replicated field trials at four locations over a three year period identified accessions with potential genes for reduced WSS infestation, increased WSS mortality, and increased indirect defense via parasitoids. Exploitation of distinct types of plant defense will expand the genetic diversity for WSS resistance currently present in elite breeding lines. 相似文献