全文获取类型
收费全文 | 71篇 |
免费 | 11篇 |
专业分类
林业 | 5篇 |
农学 | 2篇 |
18篇 | |
综合类 | 5篇 |
农作物 | 4篇 |
水产渔业 | 12篇 |
畜牧兽医 | 32篇 |
园艺 | 1篇 |
植物保护 | 3篇 |
出版年
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 5篇 |
2012年 | 2篇 |
2011年 | 7篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 3篇 |
2007年 | 6篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 6篇 |
2003年 | 2篇 |
2002年 | 6篇 |
1978年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有82条查询结果,搜索用时 281 毫秒
61.
Koji MISUMI Yuri HIRAYAMA Sachiko EGAWA Shoko YAMASHITA Hiroyoshi HOSHI Kei IMAI 《The Journal of reproduction and development》2013,59(6):520-524
This study was conducted to clarify the feasibility of newly developed vitrification
techniques for porcine embryos using the micro volume air cooling (MVAC) method
without direct contact with liquid nitrogen (LN2). Expanded blastocysts
were vitrified in a solution containing 6 M ethylene glycol, 0.6 M trehalose and 2%
(wt/vol) polyethylene glycol in 10% HEPES-buffered PZM-5. The blastocysts were
collected from gilts and vitrified using the new device (MVAC) or a Cryotop (CT).
Blastocysts were stored in LN2 for at least 1 month. After warming,
cryoprotective agents were removed using a single step. Survival of the embryos was
assessed by in vitro culture (Experiment 1) and by embryo transfer
to recipients (Experiment 2). In Experiment 1, the embryos vitrified by the MVAC or
CT and fresh embryos without vitrification (Control) were used. The survival rates of
embryos in the MVAC, CT and Control groups were 88.9% (32/36), 91.7% (33/36) and 100%
(34/34), respectively, after 48 h culture, and the hatching rates of embryos after 48
h incubation were 69.4% (25/36), 63.9% (23/36) and 94.1% (32/34), respectively. In
Experiment 2, 64 vitrified embryos were transferred to 5 recipient gilts, and 8
healthy piglets were produced from 3 recipients in the MVAC group. Similarly, 66
vitrified embryos were transferred to 5 recipient gilts, and 9 healthy piglets were
produced from 2 recipients in the CT group. These results indicated that porcine
expanded blastocysts can be cryopreserved using the MVAC method without potential
pathogen contamination from LN2. 相似文献
62.
Relationships between immunoglobulin M and immunoglobulin G or A in colostrum of Japanese black multiparous cows 下载免费PDF全文
Saya Taniguchi Mengdong Wang Shuntaro Ikeda Hidetugu Yoshioka Hiroshi Nagase Shoko Kitamura Erina Itoyama Hiroaki Murakami Miki Sugimoto Shinichi Kume 《Animal Science Journal》2016,87(4):536-540
This study was conducted to clarify the relationships among immunoglobulin (Ig)M, IgG, IgA, β‐carotene, vitamin A and α‐tocopherol contents in colostrum of 24 Japanese Black multiparous cows in order to evaluate the role of IgM on colostral IgG and IgA production. Compared with colostral IgG, colostral IgM and IgA were very low but varied widely. There was positive correlation between colostral IgM and IgG, but colostral IgM was not related with colostral IgA. There was no relationship between colostral IgM and age of cows, although colostral IgG was increased with aging. There were positive correlations among colostral β‐carotene, vitamin A and α‐tocopherol and these vitamins were positively correlated with colostral IgM and IgG. These results indicate that fat‐soluble vitamins may affect colostral IgG and IgM in cows and colostral IgG increases with the increase of colostral IgM. 相似文献
63.
Watanabe T Tokishita S Spiegelhalter F Furui S Kitta K Hino A Matsuda R Akiyama H Maitani T 《Journal of agricultural and food chemistry》2007,55(4):1274-1279
In 2005 it was reported that the genetically modified (GM) maize strain or "event" called Bt10 had been distributed inadvertently in the United States over the previous 4 years. In order to ensure that grain for food and feed production did not contain trace amounts of Bt10 maize and complied with the applicable regulation, highly sensitive and specific detection of Bt10 maize was required. Accordingly, we developed a novel qualitative PCR system for specific detection of Bt10 maize. Moreover, we amply evaluated the performance characteristics of two PCR systems, our own and the one provided by the developer of Bt10, Syngenta Co. Ltd. It was confirmed that both of the qualitative PCR systems can specifically detect Bt10 maize, and the results of a single-laboratory examination suggested that the limit of detection was approximately less than 0.05% for both methods. To evaluate the reproducibility of the methods, we organized an interlaboratory study with the participation of 6 laboratories and analysis of 240 blind test samples. In this paper, we report, for the first time, the statistical analysis of the qualitative PCR data obtained from the interlaboratory study. The results of this analysis also revealed that there was no significant difference in the sensitivity between the two aforementioned methods and that the limit of detection of both the methods was less than 0.05%. Thus, we conclude that both of the methods are equally suitable for correct identification and sensitive detection of the unapproved GM maize Bt10 event in test samples. 相似文献
64.
Conversion of natural forests to other land use results not only in a decrease of forest area but also in the degradation
of remnant forests as a habitat for forest animals. Although such degradation due to an increase of forest edges has been
studied most intensively, other factors such as forest shape may also contribute to the degradation. In this study, we compared
bird abundance and species richness between irregular-shaped and relatively continuous forests in the breeding and migratory
seasons. Since the forests were surrounded by tree plantations rather than open lands, the edge effect may have been weak
at the study site. Our results suggested that the irregular forest shape negatively affected forest bird abundance and species
richness in the breeding season, but not in the migratory season. The response of birds varied with bird traits: migrants
avoided the irregular-shaped forest, but residents did not. Among the residents, small ones preferred or tolerate the irregular-shaped
forest whereas large ones avoided it. This study indicates that careful consideration of various factors such as seasonality
and bird traits is needed to understand the consequences of land use changes on forest birds. 相似文献
65.
66.
67.
Paddy and Water Environment - The topographical features of farmland stone walls that were damaged by two heavy rains and the 2016 Kumamoto Earthquake were studied. Topographical analyses were... 相似文献
68.
The results presented here support the hypothesis that plants of the tribe Oryzeae respond enantioselectively and homogeneously to optically active 1-alpha-methylbenzyl-3-p-tolylurea (MBTU) in root growth inhibition, in contrast to Echinochloa species. The Oryzeae plants tested in this study belong to different genera (Oryza, Leersia, Chikusichloa and Zizania), to different species (O sativa, O glaberrima, O alta, O coarctata, O latifolia, O minuta, O rufipogon), to various ecospecies of Oryza (japonica, indica, japonica x indica, javanica) and to different levels of evolution [cultivated rice (O sativa and O glaberrima) and ancestral wild rice species]. In spite of their different phylogenic status and diverse sensitivity, the root growth of all members of the genus Oryza was inhibited more by R-MBTU than by S-MBTU. Zizania palustris, Z latifolia, Leersia oryzoides and Chikusichloa aquatica belonging to the tribe Oryzeae exhibited similar chiral recognition to the Oryza plants, suggesting that Oryzeae have a common chiral recognition mechanism in their response to optically active MBTUs. In contrast, Echinochloa plants (E crus-galli (L) Beauv var crus-galli and E colonum (L) Link), belonging into subfamily Panicoideae tribe Paniceae, responded in a different way, where their root growth was more sensitive to S-MBTU than to the antipodal R-MBTU. A reverse chiral response between the tribe Oryzeae and the genus Echinochloa was clearly indicated in this study. This diverse response may be relevant to Gramineae classification. 相似文献
69.
Shoko Matsukawa Kazutoshi Ueno Toshihisa Sugino Yukinori Yoshimura Naoki Isobe 《Animal Science Journal》2018,89(8):1152-1160
The objective of the present study is to examine whether colostrum whey can have an effect on immune function in goats digestive tract. Two milliliters of colostrum whey (colostrum group) or water (control group) were administrated orally to goats every day for 3 weeks. Blood was collected twice a week for 3 weeks to measure immunoglobulin A (IgA), interleukin 8 (IL‐8), and IL‐10. At the end of the experimental period, the parotid glands, oral mucosa, lingua, esophagus, jejunum, and ileum were collected for immunohistochemical detection of IgA, cathelicidin‐7, and S100A8. The ratio of the length of IgA‐positive mucosal surface in the esophagus to the total esophageal length was significantly greater in the colostrum group than in the control group. The number of IgA‐positive cells in the labial gland and ileum in the colostrum group was significantly higher than that in the control group. There were no significant differences between the colostrum and control groups in the number of cathelicidin‐7‐positive cells in the jejunum and ileum and in the number of S100A8‐positive cells in the lingua, jejunum, and ileum. These results suggest that colostrum stimulates the recruitment of plasma cells into the labial gland, which then secrete more IgA into the saliva. 相似文献
70.
Akitomo Kawasaki Shoko Okada Chunyan Zhang Emmanuel Delhaize Ulrike Mathesius Alan E. Richardson Michelle Watt Matthew Gilliham Peter R. Ryan 《Plant methods》2018,14(1):114