Structures of SAS-6 suggest its organization in centrioles     

van Breugel M  Hirono M  Andreeva A  Yanagisawa HA  Yamaguchi S  Nakazawa Y  Morgner N  Petrovich M  Ebong IO  Robinson CV  Johnson CM  Veprintsev D  Zuber B 《Science (New York, N.Y.)》2011,331(6021):1196-1199

Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.  相似文献   

Efficacy and safety of 0.0584% hydrocortisone aceponate topical spray and systemic oclacitinib combination therapy in dogs with atopic dermatitis: a randomized,double-blinded,placebo-controlled trial     

Junko Takahashi  Satoko Kanda  Ichiro Imanishi  Tadashi Hisano  Teruyasu Fukamachi  Norihito Taguchi  Shoko Momiyama  Satoshi Nishiyama  Tomoki Motegi  Keita Iyori 《Veterinary dermatology》2021,32(2):119-e25

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Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration     

Shoko Sawano  Takahiro Suzuki  Mai‐Khoi Q. Do  Hideaki Ohtsubo  Wataru Mizunoya  Yoshihide Ikeuchi  Ryuichi Tatsumi 《Animal Science Journal》2014,85(12):994-1000

Regenerative intramuscular motor‐innervation is thought to reside in the spatiotemporal expression of axon‐guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite cells, up‐regulate a secreted neural‐chemorepellent semaphorin 3A (Sema3A) during the early‐differentiation period, in response to hepatocyte growth factor (HGF) elevated in injured muscle. However, a paracrine source of the HGF release is still unknown. Very recently, we proposed a possible contribution of anti‐inflammatory macrophages (CD206‐positive M2) by showing that M2 cells infiltrate predominantly at the early‐differentiation phase (3–5 days post‐injury) and produce/secrete large amounts of HGF. However, in understanding this concept there still remains a critical need to examine if phagocytotic pro‐inflammatory macrophages (CD86‐positive M1), another activated‐phenotype still present at the early‐differentiation phase concerned, produce HGF upon muscle injury. The current immunocytochemical study demonstrated that the HGF expression is negative for M1 prepared from cardiotoxin‐injured Tibialis anterior muscle at day 5, in contrast to the intense fluorescent‐signal of M2 served as a positive control. This supplementary result advances our understanding of a spatiotemporal burst of HGF secretion from M2 populations (not M1) to impact Sema3A expression, which ensures a coordinated delay in attachment of motoneuron terminals onto damaged and generating fibers during the early phase of muscle regeneration.  相似文献   

Construction of a linkage map based on retrotransposon insertion polymorphisms in sweetpotato via high-throughput sequencing     

Yuki Monden  Takuya Hara  Yoshihiro Okada  Osamu Jahana  Akira Kobayashi  Hiroaki Tabuchi  Shoko Onaga  Makoto Tahara 《Breeding Science》2015,65(2):145-153

Sweetpotato (Ipomoea batatas L.) is an outcrossing hexaploid species with a large number of chromosomes (2n = 6x = 90). Although sweetpotato is one of the world’s most important crops, genetic analysis of the species has been hindered by its genetic complexity combined with the lack of a whole genome sequence. In the present study, we constructed a genetic linkage map based on retrotransposon insertion polymorphisms using a mapping population derived from a cross between ‘Purple Sweet Lord’ (PSL) and ‘90IDN-47’ cultivars. High-throughput sequencing and subsequent data analyses identified many Rtsp-1 retrotransposon insertion sites, and their allele dosages (simplex, duplex, triplex, or double-simplex) were determined based on segregation ratios in the mapping population. Using a pseudo-testcross strategy, 43 and 47 linkage groups were generated for PSL and 90IDN-47, respectively. Interestingly, most of these insertions (~90%) were present in a simplex manner, indicating their utility for linkage map construction in polyploid species. Additionally, our approach led to savings of time and labor for genotyping. Although the number of markers herein was insufficient for map-based cloning, our trial analysis exhibited the utility of retrotransposon-based markers for linkage map construction in sweetpotato.  相似文献   

Successful Production of Piglets Derived from Expanded Blastocysts Vitrified Using a Micro Volume Air Cooling Method without Direct Exposure to Liquid Nitrogen     

Koji MISUMI  Yuri HIRAYAMA  Sachiko EGAWA  Shoko YAMASHITA  Hiroyoshi HOSHI  Kei IMAI 《The Journal of reproduction and development》2013,59(6):520-524

This study was conducted to clarify the feasibility of newly developed vitrification techniques for porcine embryos using the micro volume air cooling (MVAC) method without direct contact with liquid nitrogen (LN₂). Expanded blastocysts were vitrified in a solution containing 6 M ethylene glycol, 0.6 M trehalose and 2% (wt/vol) polyethylene glycol in 10% HEPES-buffered PZM-5. The blastocysts were collected from gilts and vitrified using the new device (MVAC) or a Cryotop (CT). Blastocysts were stored in LN₂ for at least 1 month. After warming, cryoprotective agents were removed using a single step. Survival of the embryos was assessed by in vitro culture (Experiment 1) and by embryo transfer to recipients (Experiment 2). In Experiment 1, the embryos vitrified by the MVAC or CT and fresh embryos without vitrification (Control) were used. The survival rates of embryos in the MVAC, CT and Control groups were 88.9% (32/36), 91.7% (33/36) and 100% (34/34), respectively, after 48 h culture, and the hatching rates of embryos after 48 h incubation were 69.4% (25/36), 63.9% (23/36) and 94.1% (32/34), respectively. In Experiment 2, 64 vitrified embryos were transferred to 5 recipient gilts, and 8 healthy piglets were produced from 3 recipients in the MVAC group. Similarly, 66 vitrified embryos were transferred to 5 recipient gilts, and 9 healthy piglets were produced from 2 recipients in the CT group. These results indicated that porcine expanded blastocysts can be cryopreserved using the MVAC method without potential pathogen contamination from LN₂.  相似文献   

Ingestion of proteoglycan fraction from shark cartilage increases serum inhibitory activity against matrix metalloproteinase-9 and suppresses development of N-nitrosobis(2-oxopropyl)amine-induced pancreatic duct carcinogenesis in hamster     

Kitahashi T  Ikawa S  Sakamoto A  Nomura Y  Tsujiuchi T  Shimizu K  Sasabe S  Park EY  Nakamura Y  Tsutsumi M  Sato K 《Journal of agricultural and food chemistry》2012,60(4):940-945

A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster.  相似文献   

Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution     

Murakami KS  Masuda S  Darst SA 《Science (New York, N.Y.)》2002,296(5571):1280-1284

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Development and evaluation of event-specific qualitative PCR methods for genetically modified Bt10 maize     

Watanabe T  Tokishita S  Spiegelhalter F  Furui S  Kitta K  Hino A  Matsuda R  Akiyama H  Maitani T 《Journal of agricultural and food chemistry》2007,55(4):1274-1279

In 2005 it was reported that the genetically modified (GM) maize strain or "event" called Bt10 had been distributed inadvertently in the United States over the previous 4 years. In order to ensure that grain for food and feed production did not contain trace amounts of Bt10 maize and complied with the applicable regulation, highly sensitive and specific detection of Bt10 maize was required. Accordingly, we developed a novel qualitative PCR system for specific detection of Bt10 maize. Moreover, we amply evaluated the performance characteristics of two PCR systems, our own and the one provided by the developer of Bt10, Syngenta Co. Ltd. It was confirmed that both of the qualitative PCR systems can specifically detect Bt10 maize, and the results of a single-laboratory examination suggested that the limit of detection was approximately less than 0.05% for both methods. To evaluate the reproducibility of the methods, we organized an interlaboratory study with the participation of 6 laboratories and analysis of 240 blind test samples. In this paper, we report, for the first time, the statistical analysis of the qualitative PCR data obtained from the interlaboratory study. The results of this analysis also revealed that there was no significant difference in the sensitivity between the two aforementioned methods and that the limit of detection of both the methods was less than 0.05%. Thus, we conclude that both of the methods are equally suitable for correct identification and sensitive detection of the unapproved GM maize Bt10 event in test samples.  相似文献   

Collection,preservation and distribution of Oryza genetic resources by the National Bioresource Project RICE (NBRP-RICE)     

Yutaka Sato  Katsutoshi Tsuda  Yoshiyuki Yamagata  Hiroaki Matsusaka  Hiromi Kajiya-Kanegae  Yuri Yoshida  Ayumi Agata  Kim Nhung Ta  Sae Shimizu-Sato  Toshiya Suzuki  Misuzu Nosaka-Takahashi  Takahiko Kubo  Shoko Kawamoto  Ken-Ichi Nonomura  Hideshi Yasui  Toshihiro Kumamaru 《Breeding Science》2021,71(3):291

Biological resources are the basic infrastructure of bioscience research. Rice (Oryza sativa L.) is a good experimental model for research in cereal crops and monocots and includes important genetic materials used in breeding. The availability of genetic materials, including mutants, is important for rice research. In addition, Oryza species are attractive to researchers for both finding useful genes for breeding and for understanding the mechanism of genome evolution that enables wild plants to adapt to their own habitats. NBRP-RICE contributes to rice research by promoting the usage of genetic materials, especially wild Oryza accessions and mutant lines. Our activity includes collection, preservation and distribution of those materials and the provision of basic information on them, such as morphological and physiological traits and genomic information. In this review paper, we introduce the activities of NBRP-RICE and our database, Oryzabase, which facilitates the access to NBRP-RICE resources and their genomic sequences as well as the current situation of wild Oryza genome sequencing efforts by NBRP-RICE and other institutes.  相似文献   

Efficacy of a novel in ovo-attenuated live vaccine and recombinant vaccine against a very virulent infectious bursal disease virus in chickens     

Takashi OKURA  Hiroki OTOMO  Shoko SUZUKI  Yuji ONO  Akira TANENO  Eiji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1686

Infectious bursal disease (IBD) causes severe economic damage to the poultry industry worldwide. To prevent IBD virus (IBDV) infection, live virus vaccines have been widely used in chickens having wide-ranging levels of maternally derived antibodies. But, the risks of infection with other pathogens because of lesions related to atrophy of the bursa of Fabricius in vaccinated chickens are a concern. To resolve the problems, a recombinant turkey herpesvirus (HVT) vaccine expressing IBDV-VP2 protein (rHVT-IBD) has been developed. However, the induction of neutralizing antibodies by rHVT-IBD against a virulent IBDV might be delayed compared with that by the live IBD vaccine, leading to the high risks of IBDV infection for young chickens. To find the best selection of IBDV vaccine for the onset of immunity, we examine the protective efficacy of a novel in ovo-attenuated live IBDV (IBD-CA) vaccine and the rHVT-IBD vaccine in young chickens challenged with a very virulent IBDV (vvIBDV) strain. We show that the protective efficacy of IBD-CA vaccine was higher than that of the rHVT-IBD vaccine in 14-day-old chickens challenged with the vvIBDV strain, leading to the risk of IBDV infection for young chickens when vaccinated with rHVT-IBD. Our results suggest that farmers should select the best vaccines to maximize vaccine efficacy in consideration of the vaccine characteristics, prevalence levels of IBDV in the areas, and initial MDA levels of the chickens since the attenuated live and recombinant vaccines play a role in the different vaccine efficacies.  相似文献