Efficacy of Ovsynch Protocol with Antiprolactin Treatment for Timed Artificial Insemination During Non‐breeding Seasons in Yaks (Poephagus grunniens L.)     

M Sarkar  BK Dutta Borah  BS Prakash 《Reproduction in domestic animals》2010,45(3):393-398

An attempt was undertaken to investigate the efficacy of Ovsynch protocol for timed artificial insemination (TAI) with or without Norprolac (antiprolactin) treatment during non‐breeding season (winter months) in yaks (n = 25). During non‐breeding season, plasma prolactin profile has been reported high due to cold and nutritional stress. The Norprolac dose of i.m. administration was standardized for prolactin suppression. Three different doses viz. 2.5, 5.0 and 7.5 mg were attempted and the dose of 7.5 mg Norprolac i.m. per animal was found to be suitable for suppression of prolactin levels up to 30 h. Ovsynch treatment with Norprolac induced more number of oestrous symptoms per animal (4.8 vs 2.1), higher LH peak concentration (24.01 vs 16.16 ng/ml), longer duration of LH surge (6.8 vs 5.2 h) and higher conception rate (70 vs 30%) in Ovsynch plus Norprolac treated animals compared with animals treated with Ovsynch alone. Therefore, this study clearly indicates the opportunity for practical application of the Ovsynch plus Norprolac protocol for TAI in yaks during non‐breeding seasons.  相似文献   

Divergent development of testosterone secretion in male zebu (Bos indicus) and crossbred cattle (Bos indicus x Bos taurus) and buffaloes (Bubalus bubalis) during growth     

S. Gulia  M. Sarkar  Vijay Kumar  H. H. D. Meyer  B. S. Prakash 《Tropical animal health and production》2010,42(6):1143-1148

Delayed pubertal development and low fertility of Bos indicus x Bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (EIA) was developed using the second antibody-coating technique and testosterone-3-O-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of testosterone in blood plasma. The EIA was validated by standard criteria. Blood samples were collected by venipuncture from growing male cattle (Karan Fries and Sahiwal) and buffalo (Murrah) and testosterone was estimated using the EIA procedure. Plasma testosterone concentrations increased significantly (P < 0.05) with advancing age. Testosterone concentrations were significantly (P < 0.01) higher in Sahiwal males in comparison to Karan Fries males. The low testosterone levels in crossbred than Sahiwal could imply that crossbred males have either not stabilized genetically or not adapted well in Indian climatic conditions resulting in poor libido and poor semen quality. The low testosterone levels in Murrah buffalo males may be the possible reason for delayed maturity in this species. The direct, sensitive EIA validated for estimating the plasma testosterone concentration was reliable for studying the testosterone profile in blood plasma of males. The results suggest that there could be a requirement for higher testosterone secretion by males during early stages of growth for attaining early sexual maturity.  相似文献   

Seasonal variations in plasma glucocorticoid levels in yaks (<Emphasis Type="Italic">Poephagus grunniens L</Emphasis>.) (<Emphasis Type="Italic">Bos grunniens</Emphasis>)     

M. Sarkar  S. Bandyopadhyay  G. Krishnan  B. S. Prakash 《Tropical animal health and production》2010,42(3):421-424

The changes of glucocorticoid concentrations were measured by highly sensitive enzymeimmunoassay procedures using second antibody coating technique and cortisol -HRP amplified system for one whole calendar year in yaks. The year was divided into seasons of cool, intermediate and hot seasons. The glucocorticoid values were lower (P < .01) in hotter months than in cool and intermediate seasons. Rectal temperature and respiration rates were indicative of heat stress in the hot temperature season and gave significant negative correlation with circulating corticoid concentration.  相似文献   

Study of tuberculosis amongst cattle in Calcutta     

A N Guha  P B Sarkar 《The Indian veterinary journal》1970,47(3):196-200

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Influence of subclinical nematodosis on the kinetic disposition of fenbendazole in buffaloes     

A. C. Mathur  P. K. Sanyal  S. Sarkar 《Veterinary research communications》1995,19(1):49-57

The disposition kinetics of fenbendazole was studied in buffaloes subclinically infected with gastrointestinal nematodes. There was significantly reduced uptake of the drug in infected animals compared to uninfected controls. The pH of the duodenal liquor was highly alkaline compared to the acidic pH in uninfected animals. The egg count in the faeces never became zero though the numbers were reduced to a great extent compared to pre-treatment values. The influence of the host's physiology on the reduced bioavailability of fenbendazole is discussed.Abbreviations AUC area under the concentration-time curve - C _max peak concentration in plasma; - e.p.g. eggs per gram of faeces - ETH-OFZ ethyl oxfendazole - FBZ fenbendazole - FBZ-SO₂ fenbendazole sulphone - FEC faecal egg count - HPLC high-performance liquid chromatography - OFZ oxfendazole - T _1/2 half-life in plasma - T _max time to peak concentration in plasma  相似文献   

Effect of Intercropping Cereal, Pulse and Oilseed Crops in Redgram on Yield, Competition and Advantage     

R. K. Sarkar  D. Shit 《Journal of Agronomy and Crop Science》1993,170(3):171-176

Results of a field experiment conducted in rainy season (June to December) of 1988 and 1989 at Deochanda Experiment Station, Bihar, India, indicated that intercropping cereals, pulses and oilseeds with normal planted base crop of redgram ( Cajanus cajan [L.] Mill sp.) increased land use efficiency and gave higher total yields compared to pure cropping of redgram under rainfed conditions on upland oxisols of Bihar plateau. Cereals like maize, rice and fingermillet were more aggressive in intercropping system and they tended to have depressing effect on redgram. Intercropping of short duration pulses like blackgram and greengram and legume-oilseed crops like peanut and soybean with redgram showed balanced competitive abilities and proved more efficient in the system. Intercropping system of redgram-peanut proved to be the most efficient and productive.  相似文献   

Embryo resorption following administration of steroidal compounds to rats in mid pregnancy.          下载免费PDF全文

K Sarkar  G A Kinson    H C Rowsell 《Canadian journal of veterinary research》1986,50(3):433-437

In the course of experiments on the effects of anabolic steroids on the myocardium of rat conceptuses, we found that subcutaneous implantation of 10 mg of estradiol, Dianabol or testosterone to rats in mid pregnancy, resulted in embryo resorption. Placental tissue was identified only in estradiol-treated rats which also demonstrated a large amount of serosanguineous fluid that dilated the horns considerably. The yellow nodules of placental attachment sites were represented histologically by cellular and vascular proliferations between the inner and outer layers of the myometrium. The nodular aggregates of cells had variable features according to the steroid administered. Neither decidual cells nor metrial glands that are reported to be the constituents of placental attachment sites were seen in our material. We conclude that anabolic steroids are potent agents for embryo resorption, and that the cells in the nodules of placental attachment sites are likely to be derived from the myometrium.  相似文献   

Antiproliferative and Antimicrobial Potentials of a Lectin from Aplysia kurodai (Sea Hare) Eggs     

Rubaiya Rafique Swarna  A. K. M. Asaduzzaman  Syed Rashel Kabir  Nawshin Arfin  Sarkar M. A. Kawsar  Sultana Rajia  Yuki Fujii  Yukiko Ogawa  Keisuke Hirashima  Nanae Kobayashi  Masao Yamada  Yasuhiro Ozeki  Imtiaj Hasan 《Marine drugs》2021,19(7)

In recent years, there has been considerable interest in lectins from marine invertebrates. In this study, the biological activities of a lectin protein isolated from the eggs of Sea hare (Aplysia kurodai) were evaluated. The 40 kDa Aplysia kurodai egg lectin (or AKL-40) binds to D-galacturonic acid and D-galactose sugars similar to previously purified isotypes with various molecular weights (32/30 and 16 kDa). The N-terminal sequence of AKL-40 was similar to other sea hare egg lectins. The lectin was shown to be moderately toxic to brine shrimp nauplii, with an LC₅₀ value of 63.63 µg/mL. It agglutinated Ehrlich ascites carcinoma cells and reduced their growth, up to 58.3% in vivo when injected into Swiss albino mice at a rate of 2 mg/kg/day. The morphology of these cells apparently changed due to AKL-40, while the expression of apoptosis-related genes (p53, Bax, and Bcl-XL) suggested a possible apoptotic pathway of cell death. AKL-40 also inhibited the growth of human erythroleukemia cells, probably via activating the MAPK/ERK pathway, but did not affect human B-lymphoma cells (Raji) or rat basophilic leukemia cells (RBL-1). In vitro, lectin suppressed the growth of Ehrlich ascites carcinoma and U937 cells by 37.9% and 31.8%, respectively. Along with strong antifungal activity against Talaromyces verruculosus, AKL showed antibacterial activity against Staphylococcus aureus, Shigella sonnei, and Bacillus cereus whereas the growth of Escherichia coli was not affected by the lectin. This study explores the antiproliferative and antimicrobial potentials of AKL as well as its involvement in embryo defense of sea hare.  相似文献   

An Experimental Method to Quantify Extractable Amino Acids in Soils from Southeast China     

Xian-you CHEN  Liang-huan WU  Xiao-chuang CAO  Sarkar Animesh  Yuan-hong ZHU 《农业科学学报》2013,12(4):732-736

The extraction and comparison of soil amino acids using different extractants (deionized water, K₂SO₄, Na₂SO₄, NaCl, KCl) were reported. Results showed that 0.5 mol L^?1 K₂SO₄ with a 5 times extraction was a better method to assess the concentration of extractable amino acids in soils. The total amino acids extracted from soil planted for tea were similar to the total inorganic nitrogen. While they extracted from vegetable soil and paddy soil were much lower than the total inorganic nitrogen.  相似文献   

Body status and blood metabolites profiles during resumption of postpartum ovarian activity in yak (Poephagus grunniens)     

Vijay Paul  G. Krishnan  S. Deori  Joken Bam  P. Chakravarty  M. Sarkar 《Reproduction in domestic animals》2021,56(11):1377-1386

We examined the changes in body weight (BW), back-fat thickness (BFT) and blood metabolites in relation to postpartum (PP) ovarian activity status in twenty female yaks raised under semi-intensive system. BFT and ovarian activities, like follicle development, ovulation (OV) and corpus luteum (CL) development, were monitored from 4 to 15 weeks (wk) PP using ultrasonography. Resumption of ovarian activity was confirmed with ovulation of dominant follicle (DF) and subsequent CL development, and >1 ng/ml progesterone concentration in blood plasma sample after 1week of ovulation. Yaks were further classified as cyclic (with CL), acyclic (without CL), and cystic (with >25 mm follicular cyst; FC). Within 20 weeks PP, 60% yaks resumed cyclic ovarian activity, while 25% failed to initiate cycling activity, and 15% developed follicular cysts. In all categories of yak, BW gradually decreased (p < .05) till nadir; however, nadir reached earlier (p < .05) in acyclic yaks. BFT differed (p < .05) among the yak groups, but it tended to be higher in cyclic yaks as compared to acyclic and cystic. No difference (p > .05) in non-esterified fatty acids (NEFA) values was found among the different categories of yaks, whereas, beta-hydroxy butyrate (BHB) levels were higher in cystic animals as compared to acyclic and cyclic. Blood glucose levels decreased in all yaks during initial 2 weeks after calving. Our findings suggest that yaks with low BW, BFT and glucose levels, and higher BHB values were at risk of delayed resumption of ovarian activity and concomitant development of follicular cysts.  相似文献