白木香基因组DNA提取与ISSR反应体系的优化     

邹枚伶  夏志强  王文泉 《勤云标准版测试》2009,29(2)

白木香为瑞香科沉香属植物,是中国特有的珍贵药用植物,也是国产沉香药材唯一资源植物,现已濒临灭绝,被载入<中国植物红皮书>采用改良CTAB法提取白木香DNA较传统方法简单高效.通过琼脂糖凝胶电泳和OD260/OD280比值测定,所得基因组DNA纯度高,可作为ISSR等以PCR为基础的DNA多态性标记.通过优化影响白木香ISSR-PCR的主要参数,确立了适用于白木香的ISSR反应体系和扩增条件.结果表明在20μl反应体中,模板DNA、引物、Mg2+、dNTP和Taq DNA聚合酶5种主要成分的最适浓度分别为25 ng、0.8 μmol/L、2.5 mm01/L、0.2 mmol/L、1.0 U,扩增出足量产物至少需要35个循环.  相似文献   

新疆石河子市牛源大肠杆菌分离鉴定及对β-内酰胺类药物的耐药性分析     

许淑芸  何白雪  俞杰  马雪  胡晓彦  邹思颖  李劼  周霞  黄新  钟发刚  吴桐中  韩猛立  张星星 《中国动物检疫》2022,39(7):49-54

为调查石河子市牛源大肠杆菌对β-内酰胺类药物的耐药表型和耐药基因携带情况,采集健康牛群粪便,采用常规细菌分离鉴定方法并结合16S rRNA PCR检测方法进行分离鉴定;参考Clermont方法对分离菌株进行系统分子分群,采用PCR方法检测分离菌株的β-内酰胺类耐药基因blaCTX-M、blaOXA、blaSHV和blaTEM携带情况,通过K-B纸片法测定分离菌株耐药表型。结果显示：分离鉴定出100株牛肠道大肠杆菌分属于A、B1、C和F群;分离株对头孢唑林和阿莫西林的耐药率在50%以上,对氨苄西林、头孢氨苄与头孢吡肟的耐药率均在5%以下,所有分离株对头孢吡肟敏感;5株分离菌株检测到blaSHV基因片段,1株检测到blaCTX-M基因片段,所有菌株均未检测到blaOXA和blaTEM基因片段。结果表明,石河子市牛源正常大肠杆菌已少部分携带耐药基因,且对部分β-内酰胺类药物产生了耐药性。结果提示,应加快减抗政策的实施。  相似文献   

滇南红厚壳的造林技术研究     

任盘宇  肖文祥  邹寿青  刘祖瑜 《林业科学》2004,40(3):189-192

滇南红厚壳(Calophyllum polyanthum)是藤黄科(Guttifereae)高大乔木，高30～35m，胸径达1．2m。树冠在林中或散生时均呈塔形，树干通直圆满，自然整枝良好，枝下高高，出材率高；木材纹理直，结构较细且均匀，木质软硬适中、强度中等，锯解和旋刨性能极佳，表面平滑光洁，易干燥，不开裂，少变形，油漆后光亮红润，胶  相似文献   

新型规整填料塔间歇精馏优化操作研究     

李飘英  邹德正 《林产化学与工业》1992,12(2):126-133

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珍贵树种—裂叶榆育苗技术的研究^*          下载免费PDF全文

邹学忠  阎忠林 《林业科学研究》1992,5(2):182-187

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杉木间伐小径材材性试验研究   总被引：7，自引：0，他引：7  

洪成器  苏伟毅 《福建林业科技》1992,19(4):6-13

杉木间伐小径材材性试验结果与成材杉木比较:密度小,晚材率较低,生长较快,吸水性较强;材质为未成熟幼林材种,物理性质较差,力学强度有所降低,作为建筑结构用材时,在设计与施工中必须注意到其强度的降低,采取必要的措施。杉木间伐小径材纤维长度与成材相似,纤维宽度略宽、纤维长宽比值略小,但尚为良好的纤维原料。  相似文献   

Preliminary studies on the early quality identification ofAuricularia auricular     

Li Ling  Ding Jun-nan  Luo Li  Zou Li  Wang Xiang-li  Zhang Gui-hua 《林业研究》2005,16(1):61-64

In production industry ofAuricularia auricular, the varieties quality is most important impact factor on output. For evaluating the early quality of the edible fungus, 9 varieties ofAuricularia auricular (Au9, CF09, CF05, 29, 916, chang10, chang7, Au.Japanese and 8808) were cultured on the medium consists of potato dextrose agar (PDA) and sawdust to test their mycelium growth rate, endurance to high temperature, resistance to mildew, assimilation of nutriment, and resistance to drought. The result showed that the mycelium of Chang 10, CF09, 29 and Au.Japanese varieties had the eminent characteristics such as short lifespan, stronger assimilation of nutriment, and endurance to high temperature and steady growth. These four varieties are determined as superiority varieties ofAuricularia auricular in accordance with the research results. Foundation item: The research was supported by Science Fund of Northeast Forestry University (2004). Biography: Li Ling (1964-), female, Engineer in Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Zhu Hong  相似文献   

Evaluation of the level of threat and protective classification of the vegetation of Makehe Forest in Sanjiangyuan Nature Reserve, west China     

Dalin Zou  Youjun He  Qinwen Lin  Guofa Cui 《Frontiers of Forestry in China》2007,2(2):179-184

After investigating and studying the vegetation, we have established that Makehe Forest, in Sanjiangyuan Nature Reserve, Qinghai Province, is host to a total of 364 different species, representing 173 “genera” in 55 different vegetative families. We propose five quantifiable indices for evaluating the level of threat to these plants: 1) the distribution frequency of the “line transect”; 2) the distribution frequency of sample plots; 3) the distribution density inside sample plots; 4) the existing abundance in the forest region; and 5) plant fidelity. The results show that there are two endangered species, six vulnerable species, 12 nearly threatened species and 344 safe species. The study tried to evaluate the urgency for conserving these plants, according to coefficients of closeness to disappearance, of genetic loss and of usefulness. Results also showed that the number of species of primary concern is two; of secondary concern, nine; of the third level, 23; and of least concern, 330 species. __________ Translated from Journal of Beijing Forestry University, 2006, 28(3): 20–25 [译自: 北京林业大学学报]  相似文献   

The Asian Wapiti (Cervus elaphus)     

Zou Hongfei  D. Bruce Banwell 《林业研究》1996,7(4):46-48

The authors visited some deer farms in Northeastern China in 1991 and 1993. The seven subspecies of Asian Wapiti (Cervus elaphus) were detected by observing the animal’s appearance and analyzing the video information. The status of taxonomy research in China was also discussed.  相似文献   

A COMPARATIVE STUDY BETWEEN FAUNA OF HEILONGJAING(CHINA)AND ALBERTA PROVINCE(CANADA)     

Gao Zhongxin Gao Erhu ZouHongfci JinKun SuJinbaoNortheast Foresty UniversityJiang ZhigongAlbeta University  Canada 《林业研究》1993,4(1):64-68

Heilongjing(China)and Alberta(Canada)provinces are situated at the same lati-tude,with similar nature conditions.Mammals in Alberta account for 87 species and in Heilongj-ing,97 spesics,among which a number of orders,families and species are in common.Continentsof Eurisia and North America histoi cally were connected and separated for several times,sothere are a number of animal forms in common,among them the similarity of herbivores is rath-er high.The similarity index of cervidae is 0.54.Other orders with comparatively similarcompiosition are Insevtivora,Chiroptera and Lagomorpha.In two orders,there are no commonspecies.In these two regions,duc to historical reasons,a number of specific genera and specieswere formed.  相似文献