Assessment of biomarkers influencing treatment success on small intestinal lymphoma in dogs     

Hiroki Yamazaki  Hiroshi Sasai  Miyuu Tanaka  Toshiyuki Tanaka  Hidetaka Nishida  Shunsuke Noguchi  Mitsuru Kuwamura  Hideo Akiyoshi 《Veterinary and comparative oncology》2021,19(1):123-131

This study aimed to determine a reliable therapeutic biomarker for localized small intestinal lymphoma (SIL) in dogs based on clinical and histopathological features. We retrospectively investigated 84 dogs with localized SIL, including 36 dogs receiving surgery and 48 dogs receiving chemotherapy. The dogs receiving surgery were divided into two subgroups: 18 dogs (group 1) with overall survival (OS) <120 days (median OS) and 18 dogs (group 2) with OS ≥120 days. Correspondingly, the dogs receiving chemotherapy were divided into 24 dogs (group 3) with OS <98 days (median OS) and 24 dogs (group 4) with OS ≥98 days. Clinical, haematological, histopathological and immunohistochemical analyses were comparatively evaluated among the four subgroups. There was no significant difference in OS between the surgery and chemotherapy groups. In dogs receiving surgery, the rate of Ki67‐positive cells was significantly increased in group 1 compared to group 2 and showed no significant difference between groups 3 and 4. In dogs receiving chemotherapy, the rate of O6‐methylguanine‐DNA methyltransferase (MGMT) was significantly higher in group 3 than in group 4 and showed no significant difference between groups 1 and 2. Additionally, our data showed that OS in dogs with higher Ki67 expression might be significantly increased by chemotherapy than by surgery, that of those with higher MGMT expression might be significantly increased by surgery than by chemotherapy, and Ki67 and MGMT were independent of each other. Indices of Ki67 and MGMT are suggested therapeutic biomarkers to determine the optimal first‐line treatment for localized SIL in dogs.  相似文献   

Assessment of reference dietary amino acid pattern for juvenile red sea bream, <Emphasis Type="Italic">Pagrus major</Emphasis>     

Md. Shah?AlamEmail author  Shin ichi?Teshima  Dedy?Yaniharto  Ophirtus?Sumule  Manabu?Ishikawa  Shunsuke?Koshio 《Aquaculture International》2005,13(4):369-379

Four semi-purified diets, containing crystalline amino acids (CAAs), were fed to juvenile red sea bream, Pagrus major in order to ascertain the ideal dietary amino acid pattern for this species. A control diet containing 50% casein–gelatin as protein sources, but no CAAs were fed to the fish. The other diets contained 30% casein–gelatin and 20% CAAs. CAAs were added to diets to simulate with amino acid pattern of the red sea bream eggs protein (REP), red sea bream larvae whole body protein (RLP), red sea bream juvenile whole body protein (RJP), and brown fishmeal protein (BFP). The juveniles (average initial body weight, 1.58 ± 0.01 g) were maintained in triplicate tanks and fed twice daily for 30 days. The highest weight gain was observed in juveniles fed the RJP diet. No significant difference was observed in juveniles fed the RLP and BFP diet. Feed efficiency ratio, protein efficiency ratio and amino acid retention in the whole body were significantly (p < 0.05) affected by the simulated dietary amino acid patterns. The essential amino acid profile and A/E ratios of the whole body after the growth trial showed little difference among the dietary treatments. The results suggest that red sea bream juveniles are able to utilize high amounts of CAA in coated form. The amino acid pattern of RJP could be used as an appropriate of reference dietary amino acid for this species.  相似文献   

Epidermoid cyst of the ileum in a miniature dachshund dog     

Shunsuke Shimamura  Risa Kainuma  Ken Kimura  Yasuhiko Okamura  Saori Kobayashi  Masaaki Katayama  Reeko Sato  Jun Yasuda 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(4):366-368

A 13-year-old castrated male miniature dachshund dog was presented with chronic vomiting and diarrhea. Contrast radiography and surgical exploration revealed an ileal cyst, which was excised. A diagnosis of epidermoid cyst was made from a combination of the clinical findings and histological examination of the surgical specimen.  相似文献   

Preliminary Study of Interaction of Clarithromycin with Tacrolimus in Cats     

Masaaki KATAYAMA  Taku USHIO  Shunsuke SHIMAMURA  Yasuhiko OKAMURA  Yuji UZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1527-1529

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Assessment of cholesterol requirements in the prawn, Penaeus japonicus     

S. Teshima  M. Ishikawa  S. Koshio  & A. Kanazawa 《Aquaculture Nutrition》1997,3(4):247-253

Two feeding experiments were conducted to assess the quantitative cholesterol requirement of the juvenile prawn, Penaeus japonicus . The prawns were fed casein-based (experiment 1) and casein- or crab protein-based (experiment 2) diets with or without supplemental cholesterol. The daily increases in quantities of body cholesterol (mg kg⁻¹ prawn day⁻¹) at maximum growth and dietary cholesterol intake over a 40-day feeding period were determined. Prawns fed 5 g kg⁻¹ supplemental cholesterol, regardless of protein sources, gave the highest weight gain. Body retention efficiencies of dietary cholesterol (cholesterol retained × 100/cholesterol intake) vary among prawns fed 5 g kg⁻¹ supplemental cholesterol (diets 3, 5 and 7) but dietary cholesterol requirements expressed as mg kg⁻¹ body weight (BW) day⁻¹ were not significantly different (180 to 200 mg kg⁻¹ BW day⁻¹). Based on dietary cholesterol requirement, the optimum dietary cholesterol levels for the juvenile prawns were estimated in relation to feeding levels. When feeding levels were 3%, 5% and 7% of body weight, optimum dietary cholesterol levels were 5.0 to 6.0, 3.6 to 4.0, and 2.6 to 2.9 g kg⁻¹ of dry diet, respectively. The present study showed the advantages of determining daily cholesterol requirement (mg kg⁻¹ BW day⁻¹) at maximum growth through a factorial method in determining optimum dietary cholesterol levels in P. japonicus .  相似文献   

Effects of supplementation of coated crystalline amino acids on growth performance and body composition of juvenile kuruma shrimp Marsupenaeus japonicus   总被引：1，自引：0，他引：1  

M.S. Alam  S. Teshima  S. Koshio  & M. Ishikawa 《Aquaculture Nutrition》2004,10(5):309-316

Supplementation of crystalline amino acids (CAA) in shrimp diets is unsuccessful in most cases. In the present study, various pre‐coated CAA were developed to minimize the leaching losses from diets in water and assessed using kuruma shrimp Marsupenaeus japonicus juveniles. Experimental diets were supplemented with coated CAA or non‐coated CAA to simulate the dietary amino acid profile to that found in the whole body protein of shrimp. The four diets contained CAA coated with either carboxymethyl cellulose (CMC) (diet 1), zein (diet 2), k‐carrageenan (diet 3) or agar (diet 4), respectively. Another four diets contained CMC‐coated CAA with the addition of either zein (diet 5), casein‐gelatin (diet 6), zein and k‐carrageenan (diet 7), or casein‐gelatin and k‐carrageenan (diet 8), respectively. The 8 weeks feeding trial demonstrated that the diets containing coated CAA gave significantly higher weight gains than diets containing non‐coated CAA, with the highest value for the diet 6. The diets containing coated CAA also yielded higher feed conversion efficiency, protein efficiency ratio, and specific growth rate than diets containing non‐coated CAA. Leaching trials suggested that shrimp growth performance was improved partly due to the retardation or reduction of CAA leaching by coating them with specific binders.  相似文献   

Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter     

Weyand S  Shimamura T  Yajima S  Suzuki S  Mirza O  Krusong K  Carpenter EP  Rutherford NG  Hadden JM  O'Reilly J  Ma P  Saidijam M  Patching SG  Hope RJ  Norbertczak HT  Roach PC  Iwata S  Henderson PJ  Cameron AD 《Science (New York, N.Y.)》2008,322(5902):709-713

The nucleobase-cation-symport-1 (NCS1) transporters are essential components of salvage pathways for nucleobases and related metabolites. Here, we report the 2.85-angstrom resolution structure of the NCS1 benzyl-hydantoin transporter, Mhp1, from Microbacterium liquefaciens. Mhp1 contains 12 transmembrane helices, 10 of which are arranged in two inverted repeats of five helices. The structures of the outward-facing open and substrate-bound occluded conformations were solved, showing how the outward-facing cavity closes upon binding of substrate. Comparisons with the leucine transporter LeuT(Aa) and the galactose transporter vSGLT reveal that the outward- and inward-facing cavities are symmetrically arranged on opposite sides of the membrane. The reciprocal opening and closing of these cavities is synchronized by the inverted repeat helices 3 and 8, providing the structural basis of the alternating access model for membrane transport.  相似文献   

Effects of replacing fishmeal with fermented and non‐fermented rapeseed meal on the growth,immune and antioxidant responses of red sea bream (Pagrus major)     

Serge Dossou  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama  Mohammed F. El Basuini  Amr I. Zaineldin  Kumbukani Mzengereza  Amina Moss  Mahmoud A. O. Dawood 《Aquaculture Nutrition》2019,25(2):508-517

The effect of rapeseed meal (RM) and Aspergillus oryzae fermented rapeseed meal (RM‐Koji) on red sea bream (Pagrus major) was examined. Three groups of fish (initial weight, 4.5 ± 0.02 g) were fed a basal diet (RM0) and two test diets where half of fishmeal was replaced by RM (RM50) and RM‐Koji (FRM50) for 56 days. The obtained results showed that fish fed RM0 and FRM50 exerted significantly higher growth performance, feed utilization and haemoglobin level but lower triglyceride and cholesterol than RM50 group (p < 0.05). Interestingly, except of antiprotease activity, all the immune parameters including lysozyme, respiratory burst (NBT) and bactericidal activities were significantly increased in fish fed RM0 and FRM50 diets compared to RM50 diet (p < 0.05). In addition, malondialdehyde and reactive oxygen metabolites were significantly reduced in RM0 and FRM50 groups over RM50 group (p < 0.05). The present results suggest that fermented RM induced better growth performance and immune responses than feeding red sea bream with non‐fermented RM and both RM and RM‐Koji improved the antioxidative status of fish, making RM‐Koji an interesting candidate as a functional feed for aquatic animals.  相似文献   

Interactive effects of dietary astaxanthin and cholesterol on the growth,pigmentation, fatty acid analysis,immune response and stress resistance of kuruma shrimp (Marsupenaeus japonicus)     

Weilong Wang  Manabu Ishikawa  Shunsuke Koshio  Saichiro Yokoyama  Mahmoud A. O. Dawood  Md. Sakhawat Hossain  Amr I. Zaineldin 《Aquaculture Nutrition》2019,25(4):946-958

A 56‐day feeding trial was carried out to evaluate the interactive effects of chemically synthesized astaxanthin (Ax) and cholesterol (CHO) on the performances of kuruma shrimp juveniles. Six experimental diets containing 2 levels of Ax (0 and 0.6 g/kg diet) and 3 levels of CHO (0, 6 and 20 g/kg diet) were formulated in 2 × 3 factorial design. The results indicated that the interactions between dietary CHO and Ax were not significant on survival, growth performances, body proximate analysis, immune response and freshwater stress test. Significantly, better pigmentation performances and Ax contents of different parts of juveniles (whole body, muscle, carapace and head) were observed in higher Ax (Ax_0.6)‐supplemented groups. Meanwhile, interactions of dietary Ax and CHO were also found on colour reading parameters and whole‐body Ax contents. In addition, juveniles fed with the diet containing Ax at 0.6 g/kg diet in addition to CHO at 0, 6 and 20 g/kg diet showed better performances on digestive enzyme activities (protease, lipase and amylase) of hepatopancreas than 0 g Ax/kg diet supplemented with CHO at 0, 6 and 20 g/kg diet. It was concluded that dietary Ax and CHO functioned interactively.  相似文献   

Dietary substitution of fishmeal by alternative protein with guanosine monophosphate supplementation influences growth,digestibility, blood chemistry profile,immunity, and stress resistance of red sea bream, <Emphasis Type="Italic">Pagrus major</Emphasis>     

Md. Sakhawat Hossain  Shunsuke Koshio 《Fish physiology and biochemistry》2017,43(6):1629-1644

We determined the effects of complete fishmeal (FM) replacement by alternative protein (soy protein concentrate, SPC) with guanosine monophosphate (GMP) supplementation on growth, digestibility, immunity, blood chemistry profile, and stress resistance of juvenile red sea bream, Pagrus major. FM protein of a FM-based control diet (FM0) was replaced with 33.3 (FM33.3), 66.6 (FM66.7), and 100% (FM100) by SPC protein, and each replacement group was supplemented with 0.4% GMP to formulate four experimental diets. Each diet was randomly allocated to triplicate groups of fish (4.8 g) for 56 days. Results demonstrated that fish fed diet group FM33.3 had the significantly highest final weight, weight gain-specific growth rate, and feed intake. Meanwhile, in comparison to control, growth performance and feed utilization did not significantly differ with 66.7% FM replacement by SPC with GMP supplementation. Apparent digestibility coefficient of protein and lipid also followed a similar trend. All growth, feed utilization, and digestibility parameters were significantly lower in FM100 diet group. Blood urea nitrogen (BUN) and triglycerides (TG) increased (P < 0.05) with increasing FM replacement level by SPC. Interestingly, total cholesterol level reduces with the increasing level of FM replacement by SPC with GMP supplementation. Fish fed FM0 diet group showed the best condition of both oxidative and freshwater stress resistance. Meanwhile, FM33.3 and FM66.7 diet groups showed acceptable conditions. Innate immune responses enhanced with the increasing FM replacement level by SPC with GMP supplementation. In conclusion, FM could be replaced ≤66.7% by SPC with GMP supplementation in diets for red sea bream without any adverse effects on fish performances.  相似文献