The need for a veterinary antibiotic policy.     

K B Pedersen  F M Aarestrup  N E Jensen  F Bager  L B Jensen  S E Jorsal  T K Nielsen  H C Hansen  A Meyling  H C Wegener 《The Veterinary record》1999,145(2):50-53

The international recognition of the 'stable to table' approach to food safety emphasises the need for appropriate and safe use of antibiotics in animal production. An appropriate use of antibiotics for food animals will preserve the long-term efficacy of existing antibiotics, support animal health and welfare and limit the risk of transfer of antibiotic resistance to humans. Furthermore, it may promote consumer confidence in the veterinary use of antibiotics. In advancing these arguments, the authors of this article argue that there is a need for a visible and operational policy for veterinary use of antibiotics, paying particular attention to the policies that are being developed in Denmark.  相似文献   

Epidemiology and Inheritance of Mitral Valve Prolapse in Dachshunds     

Lisbeth H. Olsen  Merete Fredholm  Henrik D. Pedersen 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(5):448-456

One hundred ninety consecutive Dachshunds >2 years of age, including 18 families consisting of both parents and 4 or more offspring, were examined clinically and echocardiographically to study the epidemiology and inheritance of mitral valve prolapse (MVP) and other signs of myxomatous mitral valve disease in the dog. From video-recorded echocardiograms, MVP severity, jet size (color Doppler), and leaflet thickness were assessed. With regard to murmur intensity and each of these 3 echocardiographic measurements. the inheritance and the influence of age, gender, coat type, body weight, degree of obesity, heart rate, and thorax dimensions were evaluated. MVP severity correlated positively with age (P < .0001) and heart rate (P = .002), negatively with thorax circumference (P = .0005), and was related to coat type (P = .006). MVP severity progressed faster in males than in females (P = .0002). The other measures of disease severity (jet size, leaflet thickness, and murmur intensity) also correlated positively with age (all P < .0001). When compared in pairs, all 4 measures of disease severity correlated significantly with one other. Pedigree analyses did not disclose agreement with simple Mendelian models, but high disease prevalence made interpretation difficult. Mean parental MVP severity correlated significantly with MVP severity in the offspring (P = .03). The epidemiology of MVP in Dachshunds resembles that of MVP in humans, MVP severity correlates significantly with other measures of the degree of myxomatous mitral valve disease, and MVP is an inherited condition in Dachshunds. A polygenic mode of inheritance is suggested.  相似文献   

A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies.     

S Chiang  A M Dar  S M Goyal  M A Sheikh  J C Pedersen  B Panigrahy  D Senne  D A Halvorson  K V Nagaraja  V Kapur 《Journal of veterinary diagnostic investigation》2000,12(4):381-384

Avian pneumovirus (APV) infection of turkeys in Minnesota was first confirmed in March 1997. Serum samples (n = 5,194) from 539 submissions to Minnesota Veterinary Diagnostic Laboratory were tested by a modified enzyme-linked immunosorbent assay (ELISA). Of these, 2,528 (48.7%) samples from 269 submissions were positive and 2,666 (51.3%) samples from 270 submissions were negative for APV antibodies. Most positive samples were from Kandiyohi, Stearns, Morrison, and Meeker counties in Minnesota. In addition, 10 samples from South Dakota were positive. The sensitivity and specificity of the ELISA test with anti-chicken and anti-turkey conjugates were compared by testing field and experimental sera. The ELISA test with anti-turkey conjugate was more sensitive than that with anti-chicken conjugate. The ELISA tests with antigens prepared with APV strains isolated from Colorado and Minnesota were also compared. No difference was detectable. Currently, the Minnesota Veterinary Diagnostic Laboratory uses an antigen prepared from the Colorado isolate of APV and a goat anti-turkey conjugate in the ELISA test.  相似文献   

The enzyme profiles in the connective tissue attaching pin bones to the surrounding tissue is specific in farmed salmon (<Emphasis Type="Italic">Salmo salar</Emphasis>) and cod (<Emphasis Type="Italic">Gadus morhua L</Emphasis>.)     

Tram T. Vuong  Sissel B. Rønning  Svein O. Kolset  Mona E. Pedersen 《Fish physiology and biochemistry》2017,43(1):19-25

Post mortem storage is a necessary process for removal of pin bones without destruction of fillets, thereby avoiding volume and economic loss. However, the enzymes involved in loosening pin bones during storage have not been studied to a great extent. In this study, the activities and localization of MMPs in the connective tissue (CT) of pin bones dissected from fillet of salmon and cod were investigated. Interestingly, the enzyme activity profile in these two species was different during post mortem storage of fish fillets. Adding MMP inhibitor (GM6001) and serine protease inhibitor (Pefabloc) revealed different effects in the two species, suggesting different regulations in salmon and cod. In situ zymography with the same inhibitors verified MMP and serine protease activity in CT close to pin bone at early post mortem (6 h) in salmon. However, MMP inhibition was not evident in cod in this area at that time point. Immunohistochemistry further revealed MMP9 and MMP13 were located more to the outer rim of CT, facing the pin bone and adipose tissue, while MMP7 was more randomly distributed within CT in salmon. In contrast, all these three MMPs were randomly distributed in CT in cod. In summary, our study reveals different MMP enzyme profiles in salmon and cod in the pin bone area, influenced by serine proteases, and suggests that MMPs and serine proteases must be taken in consideration when studying the conditions for early pin bone removal.  相似文献   

The role of extracellular matrix components in pin bone attachments during storage—a comparison between farmed Atlantic salmon (<Emphasis Type="Italic">Salmo salar</Emphasis>) and cod (<Emphasis Type="Italic">Gadus morhua</Emphasis> L.)     

Sissel B. Rønning  Tone-Kari Østbye  Aleksei Krasnov  Tram T. Vuong  Eva Veiseth-Kent  Svein O. Kolset  Mona E. Pedersen 《Fish physiology and biochemistry》2017,43(2):549-562

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Farmers’ incentives to save water with new irrigation systems and water taxation—A case study of Serbian potato production     

Jens Erik Ørum  Mads Vejlby Boesen  Søren Marcus Pedersen 《Agricultural Water Management》2010,98(3):465-471

Drip irrigation systems and irrigation strategies like deficit irrigation (DI) and partial root drying (PRD) are potential water saving irrigation systems and strategies. This paper analyses the Serbian farmer's economic incentive to use these water saving systems and strategies instead of the present sprinkler irrigation. The analysis is a partial budgeting analysis, based on irrigation application efficiency from the literature, standard figures for power requirements, pumping efficiency and friction losses for various sources of water and pressure requirements, yields and water use from recent Serbian field experiments, as well as prices and cost structures for potatoes collected in the Belgrade region. The analysis shows that changing the present system and strategy can save a significant amount of water (almost 50%). At the same time, however, irrigation costs are also significantly increased (more than doubled), and the total production costs are increased by 10% (deficit drip irrigation) and 23% (PRD). Increased taxes on water, investment subsidies, increased energy prices, and an increased yield or yield quality may provide incentives for farmers to change to new systems and strategies. The analysis indicates that a 0.80 to 1.97 € m⁻³ water tax is needed to make deficit drip irrigation and PRD profitable. The socioeconomic cost of providing water for irrigation and the alternative value of saved water are probably not that high. Thus, water taxation may not be a socioeconomic efficient means to improve the irrigation water productivity of Serbian potato production. Drip irrigation and PRD may, however, also increase the yield quality, and a 10-23% quality premium (price increase) is needed to make deficit drip irrigation and PRD profitable.  相似文献   

Assessing peracetic acid for controlling post‐vaccination Saprolegnia spp.‐associated mortality in juvenile Atlantic salmon Salmo salar in freshwater recirculation aquaculture systems     

Christopher Good  John Davidson  David L. Straus  Susan Harper  David Marancik  Timothy Welch  Brian Peterson  Lars‐Flemming Pedersen  Christine Lepine  Natalie Redman  Thomas Meinelt  Dibo Liu  Steven Summerfelt 《Aquaculture Research》2020,51(6):2624-2627

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Effects of foam fractionation and chemical disinfection on the removal of different microalgae cultures     

Mafalda Figueiras Guilherme  Kim Joo de Jesus Gregersen  Lars‐Flemming Pedersen 《Aquaculture Research》2020,51(8):3274-3283

The recent development of recirculation aquaculture systems (RAS) potentially allows more sustainable and controlled rearing conditions. However, control of suspended microparticles and microalgae in RAS is challenging, as uncontrolled blooms of toxic algae or heterotrophic dinoflagellates can have catastrophic impacts on the production of fish. In this study, we tested the potential of protein skimmers to remove microalgae. In 100 L batch tests, protein skimmers were tested separately and in combination with ozone (O₃), ultraviolet (UV) or hydrogen peroxide (H₂O₂). Three different and distinct microalgae cultures were tested with densities from 30,000 to 120,000 algae cells/ml in a triplicated experimental setup. Trial 1 included twelve 24‐hr replicated tests where protein skimmers with air or with two levels of ozone (low and high O₃ exposure) were compared. The protein skimmer with air alone had a limited effect on the removal of microalgae compared to the untreated control algae tanks. When ozone and protein skimmers were combined, a clear additive effect was found, and all added microalgae were removed. Low O₃ dosage and protein skimmers completely removed the algae cultures within 12 hr, while more than 95% of the algae were removed within 7 hr when a higher ozone dose was used. The second trial compared the removal capacity of protein skimmers in combination with UV, H₂O₂ and O₃. These experiments showed no to limited additive effect of UV combined with protein skimming, but significantly increased removal efficiency (270% and 1,300%, respectively) was found when H₂O₂ and ozone were combined with a protein skimmer. The study showed an algae species‐specific response to a protein skimmer with ozonation and provided information on the transition from reactivity and consumption to accumulation of ozone‐produced oxidants.  相似文献   

Origin of multiexponential T(2) relaxation in muscle myowater.   总被引：5，自引：0，他引：5  

H C Bertram  A H Karlsson  M Rasmussen  O D Pedersen  S D?nstrup  H J Andersen 《Journal of agricultural and food chemistry》2001,49(6):3092-3100

To obtain a further understanding of the nature of the multiexponential T(2) relaxation seen in muscle tissue water (myowater), relaxation measurements were carried out on whole, minced, and homogenized pork of three different qualities with regard to water-holding capacity (normal, red soft exudative, and dark firm dry). Whole, minced, and homogenized pork all resulted in multiexponential T(2) relaxation (three components) independently of the quality, even though microscopic studies on homogenized meat revealed considerable disruption of the macroscopic structure. This states that the relaxation behavior in meat cannot be explained by intra-/extracellular compartmentalization of the water as suggested in earlier studies. Subsequent studies of T(2) relaxation in either whole meat, where the structure integrity was changed by the introduction of dimethyl sulfoxide (membrane disruption) or urea (protein denaturation), or minced meat with added NaCl (inter-/intraprotein interactions) lead to the suggestion that in whole meat (i) the fastest relaxation component reflects water tightly associated with macromolecules, (ii) the intermediate relaxation component reflects water located within highly organized protein structures, for example, water in tertiary and/or quaternary protein structures and spatials with high myofibrillar protein densities including actin and myosin filament structures, and (iii) the slowest relaxation component reflects the extra-myofibrillar water containing the sarcoplasmatic protein fraction. Finally, relaxation patterns in heat-set gels of superprecipitated actomyosin and bovine serum albumin similar to that identified in whole meat support the proposed nature of T(2) relaxation in muscle myowater.  相似文献   

An optimized and simplified method for analysing urea and ammonia in freshwater aquaculture systems          下载免费PDF全文

Bodil K Larsen  Johanne Dalsgaard  Per Bovbjerg Pedersen 《Aquaculture Research》2015,46(7):1608-1618

This study presents a simple urease method for analysis of ammonia and urea in freshwater aquaculture systems. Urea is hydrolysed into ammonia using urease followed by analysis of released ammonia using the salicylate‐hypochlorite method. The hydrolysis of urea is performed at room temperature and without addition of a buffer. A number of tests were performed on water samples obtained from a commercial rainbow trout farm to determine the optimal urease concentration and time for complete hydrolysis. One mL of water sample was spiked with 1.3 mL urea at three different concentrations: 50 μg L^?1, 100 μg L^?1 and 200 μg L^?1 urea‐N. In addition, five concentrations of urease were tested, ranging from 0.1 U mL^?1 to 4 U mL^?1. Samples were hydrolysed for various time periods ranging from 5 to 120 min. A urease concentration of 0.4 UmL^?1 and a hydrolysis period of 120 min gave the best results, with 99.6–101% recovery of urea‐N in samples spiked with 100 or 200 μg L^?1 urea‐N. The level of accurate quantification of ammonia using the method is 50 μg L^?1 NH₄⁺‐N, and the detection level is 5–10 μg L^?1 NH₄⁺‐N.  相似文献