Antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme     

Mine Y  Ma F  Lauriau S 《Journal of agricultural and food chemistry》2004,52(5):1088-1094

This work was aimed at the isolation, purification, and characterization of novel antimicrobial peptides from chicken egg white lysozyme hydrolysate, obtained by peptic digestion and subsequent tryptic digestion. The hydrolysate was composed of over 20 small peptides of less than 1000 Da, and had no enzymatic activity. The water-soluble peptide mixture showed bacteriostatic activity against Gram-positive bacteria (Staphylococcus aureus 23-394) and Gram-negative bacteria (Escherichia coli K-12). Two bacteriostatic peptides were purified and sequenced. One peptide, with the sequence Ile-Val-Ser-Asp-Gly-Asp-Gly-Met-Asn-Ala-Trp, inhibited Gram-negative bacteria E. coli K-12 and corresponded to amino acid residues 98-108, which are located in the middle part of the helix-loop-helix. Another novel antimicrobial peptide inhibited S. aureus 23-394 and was determined to have the sequence His-Gly-Leu-Asp-Asn-Tyr-Arg, corresponding to amino acid residues 15-21 of lysozyme. These peptides broadened the antimicrobial activity of lysozyme to include Gram-negative bacteria. The results obtained in this study indicate that lysozyme possesses nonenzymatic bacteriostatic domains in its primary sequence and they are released by proteolytic hydrolysis.  相似文献   

Ice sheet and solid Earth influences on far-field sea-level histories   总被引：1，自引：0，他引：1  

Bassett SE  Milne GA  Mitrovica JX  Clark PU 《Science (New York, N.Y.)》2005,309(5736):925-928

Previous predictions of sea-level change subsequent to the last glacial maximum show significant, systematic discrepancies between observations at Tahiti, Huon Peninsula, and Sunda Shelf during Lateglacial time (approximately 14,000 to 9000 calibrated years before the present). We demonstrate that a model of glacial isostatic adjustment characterized by both a high-viscosity lower mantle (4 x 10(22) Pa s) and a large contribution from the Antarctic ice sheet to meltwater pulse IA (approximately 15-meters eustatic equivalent) resolves these discrepancies. This result supports arguments that an early and rapid Antarctic deglaciation contributed to a sequence of climatic events that ended the most recent glacial period of the current ice age.  相似文献   

干旱胁迫对苗木蒸腾耗水日变化的影响   总被引：29，自引：4，他引：29  

招礼军  李吉跃  于界芬  Sophie Bertin 《北京林业大学学报》2003,25(3):42-47

采用BP340 0精密电子天平 ,于 2 0 0 2年夏季研究了在不同水分胁迫条件下 ,4种北方主要造林树种苗木的日蒸腾耗水速率及实际耗水量变化规律 .结果表明 ,在正常水分条件下 ,各苗木的日最大耗水速率出现在 10 :0 0— 14 :0 0之间 ,阔叶树种的蒸腾耗水速率远远大于针叶树种 ,是针叶树种的 5～ 7倍 ,苗木的耗水速率排序为 :黄栌 >火炬树 >侧柏 >油松 ;1年生的黄栌和火炬树与 5年生侧柏的日耗水量相差不大 ,是油松的 1倍 .当苗木受到干旱胁迫后 ,苗木的日最大耗水速率会提前 ,蒸腾速率迅速下降 ,但下降幅度不同 ,耗水速率排序为 :火炬树 >黄栌 >油松 >侧柏 .在中等干旱胁迫下 ,油松、火炬树、侧柏、黄栌的日平均耗水速率分别下降了 5 4 0 %、6 8 6 %、87 2 %和 90 2 % ;侧柏和黄栌之间 ,油松和火炬树之间的日耗水量基本相同 ,但侧柏和黄栌只有油松和火炬树的一半 ;干旱胁迫继续加重后 ,油松、火炬树、侧柏、黄栌的日平均耗水速率只有水分正常条件下的 15 7%、12 1%、4 3%和 9 2 % ,日耗水总量下降到 5 %～10 % ,4个树种间相差不大 .  相似文献   

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A     

Cruz-Migoni A  Hautbergue GM  Artymiuk PJ  Baker PJ  Bokori-Brown M  Chang CT  Dickman MJ  Essex-Lopresti A  Harding SV  Mahadi NM  Marshall LE  Mobbs GW  Mohamed R  Nathan S  Ngugi SA  Ong C  Ooi WF  Partridge LJ  Phillips HL  Raih MF  Ruzheinikov S  Sarkar-Tyson M  Sedelnikova SE  Smither SJ  Tan P  Titball RW  Wilson SA  Rice DW 《Science (New York, N.Y.)》2011,334(6057):821-824

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.  相似文献   

Water and nutrient use efficiency of a low-cost hydroponic greenhouse for a cucumber crop: An Australian case study   总被引：3，自引：0，他引：3  

Harsharn S. GrewalBasant Maheshwari  Sophie E. Parks 《Agricultural Water Management》2011,98(5):841-846

The Australian greenhouse industry is primarily dominated by low-cost hydroponic greenhouses for delivery of water and nutrients to plants to grow a variety of vegetable crops including cucumber and tomato. The nutrient rich drainage water from these greenhouses is generally released into the local environment causing pollution concerns. This study was initiated to investigate the opportunities in recycling drainage water to increase water and nutrient-use efficiency of hydroponic greenhouses and reduce the environmental impact of the drainage water discharge. Results indicated that a total of 4.15 ML/ha of irrigation water was applied during the 13 weeks crop growing period of which 2.56 ML/ha was drained off and 1.59 ML/ha was used to meet the crop evapotranspiration demand. The study showed that the recycling of the drainage water resulted in a 33% reduction in potable water used for irrigation in cucumber production. The drainage water contained 59% applied N, 25% applied P and 55% applied K and illustrated the potential for nutrient recovery and production cost savings through the reuse of drainage water. This case study demonstrates that some relatively simple changes in irrigation practices within greenhouse systems to recycle drainage water can considerably improve sustainability of low-cost hydroponic greenhouses and help minimise the environmental footprint of the greenhouse industry.  相似文献   

Antimicrobial potential of egg yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg     

Bourin M  Gautron J  Berges M  Attucci S  Le Blay G  Labas V  Nys Y  Rehault-Godbert S 《Journal of agricultural and food chemistry》2011,59(23):12368-12374

Chicken egg ovoinhibitor is a multidomain Kazal-type serine protease inhibitor with unknown function. Comparison of expression between different tissues indicated that ovoinhibitor is highly expressed in the magnum and liver followed by the uterus, which secrete egg white, egg yolk, and eggshell precursors, respectively. The results also revealed that ovoinhibitor expression is increased in the liver during sexual maturation followed by a subsequent decrease in mature hens. Ovoinhibitor was purified from the egg yolk plasma from nonfertilized eggs using two consecutive affinity chromatographies and gel filtration. Purified egg yolk ovoinhibitor was shown to inhibit trypsin and subtilisin. It was shown that purified egg yolk ovoinhibitor exhibited antimicrobial activities against Bacillus thuringiensis . The results suggest that this anti-protease plays a significant role in antibacterial egg defense against Bacillus spp., preventing contamination of table eggs (nonfertilized eggs) and protecting the chick embryo (fertilized eggs).  相似文献   

Application of denaturing high-performance liquid chromatography (DHPLC) for the identification of fish: a new way to determine the composition of processed food containing multiple species     

Le Fresne S  Popova M  Le Vacon F  Carton T 《Journal of agricultural and food chemistry》2011,59(23):12302-12308

The identification of fish species in transformed food products is difficult because the existing methods are not adapted to heat-processed products containing more than one species. Using a common to all vertebrates region of the cytochrome b gene, we have developed a denaturing high-performance liquid chromatography (DHPLC) fingerprinting method, which allowed us to identify most of the species in commercial crab sticks. Whole fish and fillets were used for the creation of a library of referent DHPLC profiles. Crab sticks generated complex DHPLC profiles in which the number of contained fish species can be estimated by the number of major fluorescence peaks. The identity of some of the species was predicted by comparison of the peaks with the referent profiles, and others were identified after collection of the peak fractions, reamplification, and sequencing. DHPLC appears to be a quick and efficient method to analyze the species composition of complex heat-processed fish products.  相似文献   

Additions of maize root mucilage to soil changed the structure of the bacterial community     

Emile Benizri  Christophe Nguyen  Sophie Slezack-Deschaumes 《Soil biology & biochemistry》2007,39(5):1230-1233

The organic compounds released from roots (rhizodeposits) stimulate the growth of the rhizosphere microbial community. They may be responsible for the differences in the structure of the microbial communities commonly observed between the rhizosphere and the bulk soil. Rhizodeposits consists of a broad range of compounds including root mucilage. The aim of this study was to investigate if additions of maize root mucilage, at a rate of 70 μg C g⁻¹ day⁻¹ for 15 days, to an agricultural soil could affect the structure of the bacterial community. Mucilage additions moderately increased microbial C (+23% increase relative to control), which suggests that the turnover rate of microorganisms consuming this substrate was high. Consistent with this, the number of cultivable bacteria was enhanced by +450%. Catabolic (Biolog^® GN2) and 16S-23S intergenic spacer fingerprints exhibited significant differences between control and mucilage treatments. These data indicate that mucilage can affect both the metabolic and genetic structure of the bacterial community as shown by a greater catabolic potential for carbohydrates. We concluded that mucilage is likely to significantly contribute to differences in the structure of the bacterial communities present in the rhizosphere compared to the bulk soil.  相似文献   

Plasma pro-opiomelanocortin, pro-adrenocorticotropin hormone, and pituitary adenoma size in dogs with Cushing's disease     

Granger N  de Fornel P  Devauchelle P  Segond S  Delisle F  Rosenberg D 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(1):23-28

It is difficult to predict the size of pituitary corticotroph tumors in dogs with Cushing's disease (pituitary-dependent hyperadrenocorticism [PDH]) without pituitary imaging techniques. The purpose of this study was to examine the relationship between plasma adrenocorticotropin hormone (ACTH) precursor concentration and pituitary size in dogs with Cushing's disease. Plasma concentrations of ACTH precursors (pro-opiomelanocortin [POMC]/pro-ACTH) and pituitary tumor height/brain area were measured in 36 dogs with pituitary corticotroph adenomas of various sizes. There was a correlation between tumor size (measured as the pituitary tumor height/brain area ratio [P/B]) and POMC/pro-ACTH concentration (r = .70; P < .0001). Dogs with P/B > or = 0.40 x 10(-2) mm(-1) had higher concentrations of ACTH precursors than dogs with P/B < 0.40 x 10(-2) mm(-1) (median concentration 85 pmol/L, range 15-1,350 pmol/L, n = 14 versus 15 pmol/L, range 15-108 pmol/L, n = 22; P < .0001). With a threshold of 35 pmol/L of POMC/pro-ACTH concentration, the estimated sensitivity and specificity of the kit were 93% (95% confidence interval [CI], 79-100%) and 86% (95% CI, 73-100%), respectively. We interpret these data as indicating that measurement of POMC and pro-ACTH might be of value in the characterization of tumor size in dogs with Cushing's disease. Low POMC/pro-ACTH concentrations make it unlikely that a large pituitary tumor exists in dogs with PDH.  相似文献   

Frequency of urinary tract infection among dogs with pruritic disorders receiving long-term glucocorticoid treatment     

Torres SM  Diaz SF  Nogueira SA  Jessen C  Polzin DJ  Gilbert SM  Horne KL 《Journal of the American Veterinary Medical Association》2005,227(2):239-243

OBJECTIVE: To determine frequency of urinary tract infection (UTI) among dogs with pruritic disorders that were or were not receiving long-term glucocorticoid treatment. DESIGN: Observational study. ANIMALS: 127 dogs receiving glucocorticoids for > 6 months and 94 dogs not receiving glucocorticoids. PROCEDURE: Bacterial culture of urine samples was performed in dogs receiving long-term glucocorticoid treatment, and information was collected on drug administered, dosage, frequency of administration, duration of glucocorticoid treatment, and clinical signs of UTI. For dogs not receiving glucocorticoids, a single urine sample was submitted for bacterial culture. RESULTS: Multiple (2 to 6) urine samples were submitted for 70 of the 127 (55%) dogs receiving glucocorticoids; thus, 240 urine samples were analyzed. For 23 of the 127 (18.1%) dogs, results of bacterial culture were positive at least once, but none of the dogs had clinical signs of UTI. Pyuria and bacteriuria (present vs absent) were found to correctly predict results of bacterial culture for 89.9% and 95.8% of the samples, respectively. Type of glycocorticoid, dosage, frequency of administration, and duration of treatment were not associated with frequency of UTI. None of the urine samples from dogs not receiving glucocorticoids yielded bacterial growth. The frequency of UTI was significantly higher for dogs treated with glucocorticoids than for dogs that had not received glucocorticoids. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that dogs receiving long-term glucocorticoid treatment have an increased risk of developing a UTI. On this basis, we recommend that urine samples be submitted for bacterial culture at least yearly for such dogs.  相似文献