Overexpression of xyloglucanase (AaXEG2) accelerates heteroblastic development in mangium leaves     

Sri Hartati  Enny Sudarmonowati  Tomomi Kaku  Hisato Ikegaya  Rumi Kaida  Kei’ichi Baba  Takahisa Hayashi 《Journal of Wood Science》2011,57(6):463-469

Transgenic mangium trees (Acacia mangium) overexpressing xyloglucanase (AaXEG2) were generated by spraying flower buds with Agrobacterium solution and allowing seeds to develop. The overexpression of xyloglucanase decreased xyloglucan content in the cell walls and increased stem length and diameter. The leaves of the transgenic seedlings exhibited accelerated heteroblastic development, proceeding from the stage of three bipinnate leaves to that of enlarging petiole 2 weeks earlier than wild type seedlings did.  相似文献   

Ultrastructure changes in buffalo (Bubalus bubalis) oocytes before and after maturation in vitro with sericin          下载免费PDF全文

Sri Gustina  Hasbi Hasbi  Ni Wayan Kurniani Karja  Mohamad Agus Setiadi  Iman Supriatna 《Animal Science Journal》2017,88(12):1911-1915

The aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (Bubalus bubalis ). Oocytes were matured in vitro in tissue culture medium?199 with and without sericin, and then analyzed by light and transmission electron microscopy. The experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (P  < 0.05). The immature oocytes were characterized by cortical granule clusters in the ooplasm and the absence of perivitelline space (PVS ). In contrast, the oocytes matured either with or without sericin showed the formation of PVS , erected microvilli, the migration of cortical granules to the cytoplasmic periphery, and the clear appearance of the mitochondria and vesicle in the oolemma. Interestingly, matured oocytes with sericin have smaller cortical granules than do immature oocytes (P  < 0.05). In conclusion, supplementation of 0.05% sericin in the maturation medium can enhance the maturation rate of buffalo oocytes. Several cytoplasmic ultrastructures were relocated and modulated during the in vitro maturation process of buffalo oocytes: PVS development, cortical granules migration to periphery, and mitochondria and vesicles in the cortical region. The ultrastructure was similar between the groups with and without sericin.  相似文献