苹果AFS基因的克隆与原核表达     

李萌  隋娜  张元湖  孟庆伟 《园艺学报》2006,33(1):122-124

 通过RT-PCR扩增到苹果α - 法尼烯合成酶(α-Farnesene Synthase, AFS) 基因的编码区全长,将其克隆到pET-30a ( + ) 上, 构建了该基因的原核表达载体pET-AFS, 转化大肠杆菌BL21。SDS2PAGE检测结果表明, 此基因表达了1个约66 kD的蛋白, 1 mmol/L异丙基-β - D - 硫代半乳糖苷( IPTG) 诱导该基因高效表达, 6 h表达量最高, 诱导产物以包涵体形式存在。  相似文献   

三个山茶花种(品种) 香气成分初探   总被引：12，自引：0，他引：12  

范正琪  李纪元  田敏  李辛雷  倪穗 《园艺学报》2006,33(3):592-596

 对红山茶品种‘克瑞墨大牡丹’、‘香神’、物种攸县油茶的鲜花香气成分进行了GC /MS分析, 鉴定了‘克瑞墨大牡丹’香气成分37种, ‘香神’香气27种, 攸县油茶34种, 主要以醇、醛、酯、烯、烷及芳樟醇氧化物为主。‘克瑞墨大牡丹’芳樟醇相对含量远高于其它成分, ‘香神’以壬醛和芳樟醇含量最高, 而攸县油茶却以顺- 芳樟醇氧化物Ⅱ和苯乙醇占优势。两个品种在开花前期醇类含量较高, 后期酯类成分含量增加, 攸县油茶在开花前期醇类物质含量高, 越到后期含量越低。  相似文献   

警犬训练中的强化手段     

隋吉仁  张利华 《中国工作犬业》2008,(1):35-37

一、强化的概念在警犬训练中,同一个体在相同条件下,由于强化主体的方式、方法不同,其个体行为结果的持续性和有效性也不同。《辞海》里对强化概  相似文献   

利用FTIR对苯基异氰酸酯与不同含水率纤维素反应的研究   总被引：4，自引：2，他引：4  

顾继友  高振华  李志国  李晓萍  隋淑娟 《林业科学》2004,40(2):142-147

通过FTIR红外光谱对苯基异氰酸酯与醇、水、不同含水率纤维素反应产物的研究 ,确定了产物的红外光谱吸收峰的归属。研究发现 :异氰酸酯与绝干纤维素羟基反应产物是氨基甲酸酯 ,随着纤维素含水率的增加 ,异氰酸酯与纤维素的反应越来越低 ,与水反应的比例越来越高 ;当纤维素含水率为 9 78%时 ,异氰酸酯绝大部分与水反应生成取代脲  相似文献   

我国新的林业科技范式及其影响     

黄晓玲  张卓文  隋娟娟 《湖北林业科技》2007,(1):66-69

作者应用科恩范式理论讨论了我国林业科技范式。作者认为中国20世纪的林业科技范式的根本特征是林业具有明显的不可持续性发展和生态环境破坏。21世纪新的林业科技范式的核心是林业可持续发展与生态环境建设。新的林业科技范式将对旧的林业不可持续性发展及对生态环境的破坏经营理念、生产方式、生产技术及森林利用方式进行否定,而继承和发扬以往单项先进生产技术和先进经营理念。新的林业科技革命实质就是要创建可持续发展与生态环境质量提高的林业科学技术体系。我国正在形成有中国特色的21世纪新的林业科技革命总体战略思想、战略步骤、主体工程、林业经营理念、目标及其主导技术体系。  相似文献   

加快转产项目建设积极进行产业、产品结构调整     

隋金水  孔繁斌 《林业科技情报》2000,(4)

本文结合大兴安岭地区的实际情况,以实施“天保工程”为出发点,分析了转产项目建设面临的诸多问题,并提出了改革建议和转产项目开发建设应遵循的原则及发展方向。  相似文献   

哈尔滨市三种园林害虫发生期测报的研究   总被引：9，自引：0，他引：9  

赵绥林  吕庆茹  蔡纪文 《中国森林病虫》2003,22(2):23-27

为食叶害虫黄褐天幕毛虫，榆紫叶甲，舞毒蛾的发生期测报提供物候和有效积温等几种有价值的方法。  相似文献   

Improvement of softening treatment technology of bamboo     

Rui-xiang Cheng  Qi-sheng Zhang  Shu-juan Sui 《Wood Science and Technology》2006,40(4):327-335

In order to improve the efficiency of softening bamboo block when manufacturing bamboo veneer, chemistry reagents such as NaHCO₃ are often adopted during bamboo softening treatment. But the results of Fourier transform infrared spectroscopy (FTIR) showed that the band intensity at 1,733 cm⁻¹, assigned to C=O stretching vibration in xylan, was reduced in the spectrum of softening-treated bamboo with NaHCO₃ compared with that of not softening-treated bamboo and softening treatment of bamboo without NaHCO₃. That is to say, that the hemicellulose of bamboo was destroyed after softening treatment with NaHCO₃, which meant that softening treatment of bamboo with NaHCO₃, is not a perfect softening treatment method. Thus, in this paper a softening technology at 120°C for 30 min in a closed container was adopted. The results of FTIR show that there was almost no difference in FTIR spectra between no softening treatment of bamboo and softening treatment of bamboo at 120°C for 30 min, which meant that softening treatment at 120°C for 30 min had no effect on the composition of bamboo. The results of dynamic mechanical analysis (DMA) show that T _g of not softening-treated bamboo was 120°C, while T _g of softening-treated bamboo at 120°C for 30 min was 88°C. T _g of softening-treated bamboo at 120°C for 30 min decreased by 26.7% compared with that of not softening-treated bamboo. The results of hardness show that the hardness of bamboo strip after a softening treatment for 30 min at 120°C decreased by 42.0–54.6% compared with that of not softening-treated bamboo. The results of hardness and DMA show that the effect of softening treatment of bamboo at 120°C for 30 min was resultful.  相似文献   

Analysis of three types of triterpenoids in tetraploid white birches (Betula platyphylla Suk.) and selection of plus trees     

Sui Wang  Hui Zhao  Jing Jiang  Guifeng Liu  Chuanping Yang 《林业研究》2015,(3)

Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the out...  相似文献   

咪唑型离子液体溶液中纤维素酶荧光活性测定     

刘婷婷  张鑫艺  隋小宇  陈刚  王慧玉  张杰  孙辑凯  徐涛 《东北林业大学学报》2021,49(4):103-105,120

为考查纤维素酶在离子液体溶液中的酶活,采用荧光光谱分析法对离子液体中纤维素酶的荧光活性测定,研究离子液体的浓度、类型对纤维素酶的活性影响。结果表明:纤维素酶在咪唑类离子液体溶液中荧光活性随离子液体浓度的升高而降低。离子液体为[C4 mim]Cl、[C4 mim]Br,且浓度为0.1 mol/L使纤维素酶的荧光强度减低最小,即对酶活性影响最小。  相似文献