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51.
Incidence of Clostridia in pigs 总被引:1,自引:0,他引:1
N G Narayan 《Acta veterinaria Academiae Scientiarum Hungaricae》1967,17(2):179-182
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Senthilkumar N Kataria JM Koti M Dhama K Dash BB 《Veterinary research communications》2004,28(5):447-453
Egg drop syndrome 1976 (EDS-76) is caused by a haemagglutinating adenovirus belonging to group III of the genus Aviadenovirus in the family Adenoviridae. All isolates are serologically identical, but have been divided into three groups based on restriction endonuclease (RE) analysis. In this study the viral DNA of various Indian EDS-76 viral isolates (CEDS-A, CEDS-B, EDS-M, EDS-ML, EDS-1/AD/86, EDS-KC and QEDS) obtained from different avian species and different geographical regions were digested with restriction endonucleases viz., EcoRI, BamHI, HindIII and PstI. The results showed that one Indian isolate obtained from duck (DEDS-KC) was different from all other chicken and quail counterparts. All other isolates were identical to the reference viral strain BC-14, which belong to group I of EDS-76 viruses. The duck isolate EDS-KC could not be placed in any of the three groups reported earlier. 相似文献
55.
Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR 总被引:2,自引:0,他引:2
Tiwari AK Kataria RS Nanthakumar T Dash BB Desai G 《Comparative immunology, microbiology and infectious diseases》2004,27(3):163-169
Degenerate primers based RT-PCR (previously described by [Avian Dis 26 (1997) 837]) has been used for the detection and differentiation of Newcastle disease (ND) viruses. Two sets of primers (A+B and A+C), with common forward primer and distinct reverse degenerate primers, designed from fusion protein gene encoding for cleavage site, could differentiate virulent and avirulent Newcastle disease viruses (NDV). Both sets of primers amplified "F" gene sequence of virulent (velogenic and mesogenic) viruses, whereas in avirulent strains, amplification was only with primer set A+C. Total 10 NDV isolates and two clinical samples including both known and unknown pathotypes, were checked. Based on amplification results 5 viruses were found to be virulent type and 6 as avirulent with one of the two clinical samples, earlier positive by RT-PCR using non-degenerate "F" gene specific primers was found negative in this study. The technique has been found to be a simple and quick for the detection and differentiation of virulent and avirulent NDV, which is important for control of the disease in the events of the outbreaks. 相似文献
56.
Oral administration of the ethanolic extract (200 and 400 mg/kg, p.o) and its fractions (200 mg/kg each) of the aerial parts of Cleome rutidosperma produced significant analgesic activity in acetic acid-induced writhing and tail immersion tests, anti-inflammatory effect against carrageenin induced inflammation and adjuvant induced polyarthritis and antipyretic activity against yeast-induced pyrexia. Fractionation of the ethanolic extract potentiated the activities. 相似文献
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cis- and trans-acting transcriptional regulation of visna virus 总被引:23,自引:0,他引:23
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A laser method based upon carbon ion implantation and pulsed laser melting of copper has been used to produce continuous diamond thin film. Carbon ions were implanted with ion energies in the range of 60 to 120 keV, and doses of 1.0 x 10(18) to 2.0 x 10(18) ions cm(-2). The ion-implanted specimens were treated with nanosecond excimer laser pulses with the following parameters: energy density, 3.0 to 5.0 J cm(-2); wavelength, 0.308 microm; pulse width, 45 nanoseconds. The specimens were characterized with scanning electron microscopy (SEM), x-ray diffraction, Rutherford backscattering/ion channeling, Auger, and Raman spectroscopy. The macroscopic Raman spectra contained a strong peak at 1332 cm(-1) with full width at half maximum of 5 cm(-1), which is very close to the quality of the spectra obtained from single-crystal diamond. The selected area electron diffraction patterns and imaging confirmed the films to be defect-free single crystal over large areas of up to several square micrometers with no grain boundaries. Low voltage SEM imaging of surface features indicated the film to be continuous with presence of growth steps. 相似文献
60.
The Burdekin Delta is a major irrigation area situated in the dry tropics of North Queensland. It is unique in that (i) it overlies shallow groundwater systems that serve as a major water supply for the irrigation of sugarcane, and (ii) it is adjacent to the world heritage listed Great Barrier Reef. Water management practices include large recharge pits and surface spreading of water to assist with replenishment of the groundwater. This has been useful in maintaining groundwater levels to help control seawater intrusion. This technique, however, can be costly and ineffective in unconfined aquifer systems, which are subjected to large amounts of groundwater pumping for irrigation. There are more than 1800 production bores currently used for irrigation in the Burdekin Delta and the large volumes of water extracted have at times lowered the regional water tables and made it difficult to control seawater intrusion. 相似文献