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21.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):331-336
Infective endocarditis (IE) in dogs with cardiac shunts has not been reported previously.
However, we encountered a dog with concurrent patent ductus arteriosus (PDA) and IE. The
dog was a 1-year-old, 13.9-kg female Border collie and presented with anorexia, weight
loss, pyrexia (40.4°C) and lameness. A continuous murmur with maximal intensity over the
left heart base (Levine 5/6) was detected on auscultation. Echocardiography revealed a PDA
and severe aortic stenosis (AS) caused by aortic-valve vegetative lesions.
Corynebacterium spp. and Bacillus subtilis were
isolated from blood cultures. The dog responded to aggressive antibiotic therapy, and the
PDA was subsequently surgically corrected. After a series of treatments, the dog showed
long-term improvement in clinical status. 相似文献
22.
Arisa SUGIMOTO Hitomi TSUCHIDA Mayuko NAGAE Naoko INOUE Yoshihisa UENOYAMA Hiroko TSUKAMURA 《The Journal of reproduction and development》2022,68(3):190
Reproductive function is suppressed during lactation owing to the suckling-induced suppression of the kisspeptin gene (Kiss1) expression in the arcuate nucleus (ARC) and subsequent suppression of luteinizing hormone (LH) release. Our previous study revealed that somatostatin (SST) neurons mediate suckling-induced suppression of LH release via SST receptor 2 (SSTR2) in ovariectomized lactating rats during early lactation. This study examined whether central SST-SSTR2 signaling mediates the inhibition of ARC Kiss1 expression and LH release in lactating rats during late lactation and whether the inhibition of glutamatergic neurons, stimulators of LH release, is involved in the suppression of LH release mediated by central SST-SSTR2 signaling in lactating rats. A central injection of the SSTR2 antagonist CYN154806 (CYN) significantly increased ARC Kiss1 expression in lactating rats on day 16 of lactation. Dual in situ hybridization revealed that few ARC Kiss1-positive cells co-expressed Sstr2, and some of the ARC Slc17a6 (a glutamatergic neuronal marker)-positive cells co-expressed Sstr2. Furthermore, almost all ARC Kiss1-positive cells co-expressed Grin1, a subunit of N-methyl-D-aspartate (NMDA) receptors. The numbers of Slc17a6/Sstr2 double-labeled and Slc17a6 single-labeled cells were significantly lower in lactating dams than in non-lactating rats whose pups had been removed after parturition. A central injection of an NMDA antagonist reversed the CYN-induced increase in LH release in lactating rats. Overall, these results suggest that central SST-SSTR2 signaling, at least partly, mediates the suppression of ARC Kiss1 expression and LH release by inhibiting ARC glutamatergic interneurons in lactating rats. 相似文献
23.
An experiment was conducted with 16 Japanese Black cows to determine the effects of different days on feed (89, 208, 271 and 341 days), inclusion levels of grain in concentrate (60% vs. 40%; dry matter (DM) basis) and roughage sources (hay vs. rice straw) on finishing performance. Additional four cows were slaughtered at 0 days on feed to obtain non‐fed carcass data. Maximum carcass weights were observed in cows fed for 271 days; an increase in carcass weight of only 14 kg was shown from 208 days to 271 days. The marbling score increased (P < 0.05) with days on feed. The percentage of fat in the subprimal rib linearly increased (P < 0.05) with days on feed. The percentage of lean meat was higher (P < 0.05) for cows fed rice straw than for cows fed hay as a roughage source. Increasing the days on feed decreased (P < 0.05) the yellowness of the fat. The effects of the inclusion levels of grain in concentrate on the finishing performance of cows were lower than the other two factors. In conclusion, feeding improves carcass values, but feeding over 208 days is an inefficient system of production in cows. 相似文献
24.
Khethiwe MTSHALI Zamantungwa T. H. KHUMALO Ryo NAKAO Dennis J. GRAB Chihiro SUGIMOTO Oriel M. M. THEKISOE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1573-1579
Ticks carry and transmit a remarkable array of pathogens including bacteria, protozoa and
viruses, which may be of veterinary and/or of medical significance. With little to no
information regarding the presence of tick-borne zoonotic pathogens or their known vectors
in southern Africa, the aim of our study was to screen for Anaplasma
phagocytophilum, Borrelia burgdorferi, Coxiella
burnetii, Rickettsia species and Ehrlichia
ruminantium in ticks collected and identified from ruminants in the Eastern
Cape, Free State, KwaZulu-Natal and Mpumalanga Provinces of South Africa. The most
abundant tick species identified in this study were Rhipicephalus evertsi
evertsi (40%), Rhipicephalus species (35%), Amblyomma
hebraeum (10%) and Rhipicephalus decoloratus (14%). A total of
1634 ticks were collected. DNA was extracted, and samples were subjected to PCR
amplification and sequencing. The overall infection rates of ticks with the target
pathogens in the four Provinces were as follows: A. phagocytophilum, 7%;
C. burnetii, 7%; E. ruminantium, 28%; and
Rickettsia spp., 27%. The presence of B. burgdorferi
could not be confirmed. The findings of this study show that zoonotic pathogens are
present in ticks in the studied South African provinces. This information will aid in the
epidemiology of tick-borne zoonotic diseases in the country as well as in raising
awareness about such diseases in the veterinary, medical and tourism sectors, as they may
be the most affected. 相似文献
25.
Hiroshi SUNAHARA Yoko FUJII Keisuke SUGIMOTO Takuma AOKI Gou SUGAHARA Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):95-99
A two-month-old female Chihuahua was diagnosed as severe pulmonary valvular
stenosis (PS). Although balloon valvuloplasty (BV) was successfully performed, restenosis
was observed 19 months after the procedure. Euthanasia was chosen due to low output
syndrome during the surgical repair attempted when the dog was 5 years old. Postmortem
examination revealed markedly thickened pulmonary valve due to the increase of
extracellular matrix which might be produced by increased α smooth muscle actin-positive
myofibroblasts. The thickening of the valve was associated with restriction of the valve’s
motion, resulting in restenosis in the present case. This is the first case report
documented histopathological and immunohistochemical findings of the restenotic pulmonary
valve in dogs with PS after BV. 相似文献
26.
Keisuke SUGIMOTO Yoko FUJII Hiroshi SUNAHARA Takuma AOKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1101-1108
Hypertrophic cardiomyopathy (HCM) in cats is characterized by concentric left
ventricular (LV) hypertrophy and both diastolic and systolic dysfunction. Although
impaired cardiac function detected by tissue Doppler imaging (TDI) in cats with HCM was
previously reported, reference ranges of TDI in normal cats and cats with HCM have been
reported as widely variable. Two-dimensional speckle tracking echocardiography (STE) was
useful for assessment of cardiac function in human patients with HCM, but clinical utility
was not known in cats. The aim of this study was to assess global and segmental LV
myocardial function using STE in cats with HCM whose TDI variables were within the
reference range. A total of 35 cats of different breeds were enrolled in this study. The
HCM group (n=22) was cats diagnosed as HCM without left atrial enlargement and with normal
TDI measurements. HCM cats were further divided into a segmental hypertrophy (S-HCM) group
and a diffuse hypertrophy (D-HCM) group. The control group consisted of 13 clinically
healthy cats. No cats in any group showed any clinical symptoms. Conventional
echocardiography, TDI, and global and segmental STE indices were evaluated and compared
between groups. Only the longitudinal strain rate during early diastole was significantly
decreased in both HCM groups, even in all segments including those without hypertrophy in
S-HCM group. This study suggests that STE parameters are the more sensitive variables
compared with conventional TDI parameters to detect early myocardial diastolic dysfunction
in cats with HCM. 相似文献
27.
Latex agglutination test for canine parvovirus 总被引：1，自引：0，他引：1
TAKESHI SANEKATA TATSUYA SUGIMOTO SHINOBU UEDA MISAO TSUBOKURA YOSHIHISA YAMANE† MEGUMI SENDA‡ 《Australian veterinary journal》1996,73(6):215-217
Canine parvovirus (CPV) was detected in faeces from dogs with diarrhoea by a specific slide agglutination test using latex particles coated with anti-CPV monoclonal antibody (LA-anti-CPV). The agglutination of LA-anti-CPV with CPV on a glass slide was evident macroscopically within 2 min. The sensitivity of the latex agglutination (LA) test was similar to that of the hemagglutination test. The LA test is available for the rapid diagnosis of CPV infection at an animal hospital. 相似文献
28.
Interannual, decadal and interdecadal variations in summer plankton biomass during 1954–1994 in the whole subarctic Pacific and Bering Sea were compared among regions as well as with climatic and oceanographic conditions. The zooplankton biomass and chlorophyll concentration during the mid 1960s to early 1970s in the central and western subarctic Pacific were a few times higher than those in the preceding and following decades. The values in the eastern Bering Sea and eastern subarctic Pacific also increased in the mid 1960s, but remained at an elevated level until the end of the 1980s. These decades of higher and mid plankton biomass levels during the mid 1960s to early 1970s and mid 1970s to late 1980s correspond to the period of positive and negative values of the Northern Hemisphere zonal index (NHZI), respectively. In the decadal scale, one can see a significant positive correlation between the summer plankton biomass and the wind speed during winters in the eastern Bering Sea. The effect of grazing by biennially fluctuating Asian pink salmon on zooplankton biomass and its effect on chlorophyll concentration in the central subarctic Pacific is also significant. 相似文献
29.
Isolation of genes showing increased expression during bovine adipocyte differentiation 总被引：1，自引：0，他引：1
Tadashi YAMASAKI Miho INOUE-MURAYAMA Koji TAHARA Shoichi TAKANO Akinori SUGIYAMA Tomohito ITOH Akiko TAKASUGA Yoshikazu SUGIMOTO Michael T. ROZE Hisashi ASO Shin'ichi ITO 《Animal Science Journal》2005,76(5):479-489
The adipocyte is important not only for the storage of excess energy as fat, but also for the secretion of homeostatic factors. Gene expression profiles during adipocyte differentiation have been reported previously for mouse 3T3‐L1 cells. However, the profiles of adipogenic gene expression in mice and cattle may be different because several metabolic pathways of the ruminant adipose tissue are different from those of non‐ruminants. The gene expression profile in a clonal bovine intramuscular preadipocyte cell line during adipogenesis was examined using the polymerase chain reaction‐subtraction method. Six hundred and twenty‐one clones, which were expressed at an early stage of differentiation, from the preadipocyte to adipocyte, were isolated and characterized. Further detailed studies were carried out for 86 selected genes using northern blotting. Ten genes were found to be highly expressed after differentiation of bovine intramuscular preadipocyte cells. In particular, the expression profiles of genes for stearoyl CoA desaturase and FK506 binding protein were quite different from the time course of differentiation of that seen in the 3T3‐L1 cells reported previously. In addition, these genes were assigned to bovine chromosomes using a bovine/hamster somatic cell hybrid panel and public database. 相似文献
30.
Masahito SUGIMOTO Masayo KANAMOTO Takuya CHIBA Hisashi HIDARI Katsuya KIDA Waka SAITO Motoki OOI Yukinobu SATO Toshiro SAITO 《Animal Science Journal》2008,79(4):443-452
Six Japanese Black (Wagyu) steers (average initial weight 467 ± 45 kg) fitted with a ruminal cannula were used in a split‐plot design experiment comprising a 3 × 3 Latin square design (whole‐plot) and a randomized block design (subplot). The whole‐plot treatments were three different feeding levels of urea‐treated potato pulp (PP) silage‐based concentrate: 1.00%, 1.75% and 2.50% of body weight (BW) (on a dry matter (DM) basis). The subplot treatments consisted of the concentrate formulated to contain either soybean meal (SBM) as a rapidly rumen‐degraded protein source or corn gluten meal (CGM) as a slowly degraded protein source. Dry matter intake tended to be lower (P = 0.071) for CGM (8.9 kg/day) than for SBM (9.4 kg/day). Protein sources had no significant effect on digestibility and in situ degradation. Ruminal ammonia nitrogen (NH3‐N) was lower (P = 0.033) for CGM (7.5 mg/dL) than for SBM (9.5 mg/dL). Protein sources did not affect ruminal pH and the total volatile fatty acids (VFA) concentrations. The molar proportions of ruminal acetate and valerate were higher (P = 0.032) for CGM than for SBM. The maximum daily intake of the PP silage‐based concentrate expressed as a percentage of BW was approximately 1.4% of BW. Dry matter intake was higher (P = 0.046) for steers fed at 1.0% of BW of the PP silage‐based concentrate than for steers fed at 1.75% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate had no effect on DM and nutrients digestibility, except for crude protein (CP) digestibility. CP digestibility tended to be lower (P = 0.071) for steers fed at 1.75% of BW of the PP silage‐based concentrate than for steers fed at 1.0% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate also did not affect the in situ degradation parameter of hay and PP silage. The feeding levels of the PP silage‐based concentrate did not affect ruminal pH, NH3‐N and total VFA concentrations. The molar proportion of acetate was highest for steers fed at 1.0% of BW of the concentrate. In conclusion, in the urea‐treated PP silage‐based concentrate, CGM seems to be more effective than SBM for stabilizing the ruminal NH3‐N concentration and to be advantageous for fiber digestion in the rumen. The feeding levels of the PP silage‐based concentrate did not change the amount of VFA production in the rumen and the DM digestibility. 相似文献