Utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of walleye pollock Theragra chalcogramma   总被引：3，自引：0，他引：3  

Nobuyuki Ohkubo  Sayumi Sawaguchi  Tomonori Hamatsu  Takahiro Matsubara 《Fisheries Science》2006,72(3):620-630

ABSTRACT:   To elucidate the utilization of the major yolk nutrient stocks in eggs and larvae of walleye pollock Theragra chalcogramma , the contents of free amino acids (FAA), the major yolk protein (180 kDa lipovitellin originated from vitellogenin B in ovulated eggs: oLv B), and lipids were measured. Most eggs hatched 18 days after fertilization at 5°C, and all larvae absorbed almost all their yolk mass by 28 days. The total FAA content showed no change during the first 6 days, and then decreased to 28% of the initial level by 18 days. The oLv B contents, measured by an enzyme-linked immunosorbent assay using a specific antiserum against oLv B, gradually decreased from 6 to 18 days, followed by a rapid decline. The content of phospholipids (PL) and triacylglycerols (TG) showed no marked change until hatching, and then decreased until disappearance of yolk sac. From these results, it is proposed that there are two main periods for nutrient utilization in embryos and larvae of walleye pollock. In the first period, FAA was mainly utilized until 18 days after fertilization. Active utilization of oLv B and lipids (PL and TG) instead of FAA occurred during the second period from 18 to 28 days.  相似文献   

Estrogenic activity in coastal areas around Japan evaluated by measuring male serum vitellogenins in Japanese common goby Acanthogobius flavimanus   总被引：1，自引：0，他引：1  

Nobuyuki  OHKUBO  Kazuhiko  MOCHIDA  Shinji  ADACHI  Akihiko  HARA  Komei  HOTTA  Yukio  NAKAMURA  Takahiro  MATSUBARA 《Fisheries Science》2003,69(6):1135-1145

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Balenine,imidazole dipeptide,induces activation of superoxide dismutase in myotubes     

Yang  Min  Sun  Luchuanyang  Kawabata  Yasunosuke  Maegawa  Takahiro  Taniyama  Shigeto  Tachibana  Katsuyasu  Hirasaka  Katsuya 《Fisheries Science》2021,87(3):403-409

Balenine is one of the endogenous imidazole dipeptides. It is composed of beta-alanine and 3-methyl-l-histidine, which exist mainly in the muscles and the brain. The exact biological properties of balenine are still not well known, although the antioxidant activity of carnosine, another imidazole dipeptide, is known. In this study we investigated whether balenine exhibits antioxidant activity. It was found to decrease the superoxide anion (O₂⁻) and increased hydrogen peroxide (H₂O₂) generation. We found that SOD activity increased in balenine-treated C2C12 myotubes, although balenine did not increase expression of SOD mRNA. On the other hand, there were no changes in other antioxidant enzymes, CAT and GPX activity, and mRNA levels. In an in vitro assay, the direct activation of SOD treated by balenine was significantly higher than with carnosine treatment. Moreover, balenine constituent amino acids did not have the ability to activate SOD. Our results suggest that balenine contributes to antioxidant effects through activation of SOD.

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Nondestructive detection of cracks near the surface of wooden boards by dynamic heat dissipation     

Minoru Aoyagi  Takefumi Hiraguri  Takahiro Ueno 《Wood Science and Technology》2014,48(4):773-786

A method to detect cracks near the surface of wooden boards has been developed where the change in surface temperature of the wood sample is monitored using an infrared camera following momentary heating by a flash. Cylindrical holes simulating cracks were drilled into the wood samples, and blackbody paint was painted onto the surface of the samples to assist flash absorption. This method uses the dynamic heat transport process from the blackbody paint to the surface of the wood sample to the cracks over a short timescale. The theoretical foundations of the observation method were outlined, and the technique was verified in experiments with samples of Cercidiphyllum japonicum, Western red cedar, Japanese cedar, Balsa, and medium-density fiberboard. The developed technique is able to detect the presence of holes located near the surface of some samples. However, this method could not detect the presence of holes in Balsa nor holes located deeper than 1 mm from the surface of the sample. A theoretical analysis of these phenomena was provided to help interpreting them.  相似文献   

Collection,mapping, and annotation of over 28,000 cDNA clones from japonica rice   总被引：2，自引：0，他引：2  

Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team  Kikuchi S  Satoh K  Nagata T  Kawagashira N  Doi K  Kishimoto N  Yazaki J  Ishikawa M  Yamada H  Ooka H  Hotta I  Kojima K  Namiki T  Ohneda E  Yahagi W  Suzuki K  Li CJ  Ohtsuki K  Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group  Otomo Y  Murakami K  Iida Y  Sugano S  Fujimura T  Suzuki Y  Tsunoda Y  Kurosaki T  Kodama T  Masuda H  Kobayashi M  Xie Q  Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379

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Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop   总被引：1，自引：0，他引：1  

Hirata H  Yoshiura S  Ohtsuka T  Bessho Y  Harada T  Yoshikawa K  Kageyama R 《Science (New York, N.Y.)》2002,298(5594):840-843

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Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus     

Watanabe T  Tomizawa S  Mitsuya K  Totoki Y  Yamamoto Y  Kuramochi-Miyagawa S  Iida N  Hoki Y  Murphy PJ  Toyoda A  Gotoh K  Hiura H  Arima T  Fujiyama A  Sado T  Shibata T  Nakano T  Lin H  Ichiyanagi K  Soloway PD  Sasaki H 《Science (New York, N.Y.)》2011,332(6031):848-852

Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.  相似文献   

Testicular development and serum steroid hormone levels in captive male spotted halibut Verasper variegatus     

Yasunori  KOYA  Hiroki  WATANABE  Kiyoshi  SOYANO  Kengo  OHTA  Masato  ARITAKI  Takahiro  MATSUBARA 《Fisheries Science》2003,69(4):792-798

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Effect of the strain combination of the donor and recipient on the production efficiency of W‐bearing sperm in mixed‐sex germline chimeric chickens     

Hiroshi KAGAMI  Jun YASUDA  Takahiro TAGAMI  Mitsuru NAITO  Yuko MATSUBARA  Takashi HARUMI  Takamasa NOGUCHI  Yasuhiro YAMAMOTO  Taikai TAKAHASHI  Junichi MATSUYAMA  Hiroyuki KOMATSU  Tamao ONO 《Animal Science Journal》2002,73(6):453-456

Effect of the strain combination of the donor and recipient on production efficiency of W‐bearing sperm in mixed‐sex chimeric testes was analyzed. The combinations of the donors and recipients were White Leghorn (WL) and Rhode Island Red (RIR), and vice versa. Generated mixed‐sex chimeras that had the male phenotype at sexual maturity were classified into four groups: (1) a female WL donor and a male RIR recipient; (2) a male WL donor and a female RIR recipient; (3) a female RIR donor and a male WL recipient; (4) a male RIR donor and a female WL recipient. The mean number of W‐bearing sperm detected by in situ hybridization among 10 000 sperm observed were 147, 165, 30 and 45 in groups 1, 2, 3 and 4, respectively. The numbers in groups 1 and 2 were both significantly higher than those of groups 3 and 4 (P < 0.05). The combination of a WL donor and a RIR recipient produced W‐bearing sperm more efficiently than the reverse combination.  相似文献   

Fixation to the canine bone of artificial implant with new surface structure     

Seno T  Izumisawa Y  Nishimura I  Maehara S  Kushiro T  Umar MA  Wakaiki S  Yamashita K  Kotani T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):315-318

Screw and laser (SL) column by making screw threads and forming small holes using laser irradiation on the base metal and conventional beads coating (BC) columns were embedded into the shaft of canine femurs, and compared the implant fixation to the host bone. The interfacial strength in SL columns was almost equivalent as BC columns, and bone-column contact rate was higher than BC columns significantly at twelve weeks after implantation. The newly devised SL surface had almost equivalent bone fixation strength comparable to the conventional BC surface. Also, this surface should provide a useful porous surface for use in artificial joints since there is no risk of surface structure detachment.  相似文献