Effects of Oral Administration of Fucoidan Extracted from Cladosiphon okamuranus on Tumor Growth and Survival Time in a Tumor-Bearing Mouse Model     

Kazuo Azuma  Toshitsugu Ishihara  Hiroyuki Nakamoto  Takao Amaha  Tomohiro Osaki  Takeshi Tsuka  Tomohiro Imagawa  Saburo Minami  Osamu Takashima  Shinsuke Ifuku  Minoru Morimoto  Hiroyuki Saimoto  Hitoshi Kawamoto  Yoshiharu Okamoto 《Marine drugs》2012,10(10):2337-2348

We evaluated the anti-tumor activities of the oral administration of fucoidan extracted from Cladosiphon okamuranus using a tumor (colon 26)-bearing mouse model. The materials used included low-molecular-weight fucoidan (LMWF: 6.5–40 kDa), intermediate-molecular-weight fucoidan (IMWF: 110–138 kDa) and high-molecular-weight fucoidan (HMWF: 300–330 kDa). The IMWF group showed significantly suppressed tumor growth. The LMWF and HMWF groups showed significantly increased survival times compared with that observed in the control group (mice fed a fucoidan-free diet). The median survival times in the control, LMWF, IMWF and HMWF groups were 23, 46, 40 and 43 days, respectively. It was also found that oral administration of fucoidan increased the population of natural killer cells in the spleen. Furthermore, from the results of the experiment using Myd-88 knockout mice, it was found that these effects are related to gut immunity. These results suggest that fucoidan is a candidate anti-tumor functional food.  相似文献   

Total Synthesis and Absolute Configuration of the Marine Norditerpenoid Xestenone     

Koichiro Ota  Takao Kurokawa  Etsuko Kawashima  Hiroaki Miyaoka 《Marine drugs》2009,7(4):654-671

Xestenone is a marine norditerpenoid found in the northeastern Pacific sponge Xestospongia vanilla. The relative configuration of C-3 and C-7 in xestenone was determined by NOESY spectral analysis. However the relative configuration of C-12 and the absolute configuration of this compound were not determined. The authors have now achieved the total synthesis of xestenone using their developed one-pot synthesis of cyclopentane derivatives employing allyl phenyl sulfone and an epoxy iodide as a key step. The relative and absolute configurations of xestenone were thus successfully determined by this synthesis.  相似文献   

Functional characterization of putative G protein-coupled receptors in the tomato pathotype of Alternaria alternata     

Kazumi Takao  Yasunori Akagi  Takashi Tsuge  Motoichiro Kodama 《Journal of General Plant Pathology》2016,82(2):82-88

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Retort beef aroma that gives preferable properties to canned beef products and its aroma components          下载免费PDF全文

Koshiro Migita  Takao Iiduka  Kie Tsukamoto  Sayuri Sugiura  Genichiro Tanaka  Gousuke Sakamaki  Yasufumi Yamamoto  Yusuke Takeshige  Toshio Miyazawa  Ayako Kojima  Tomoko Nakatake  Akihiro Okitani  Masanori Matsuishi 《Animal Science Journal》2017,88(12):2050-2056

The objective of this study is to identify the properties and responsible compounds for the aromatic roast odor (retort beef aroma) that commonly occurs in canned beef products and could contribute to their palatability. The optimal temperature for generating retort beef aroma was 121°C. An untrained panel evaluated both uncured corned beef and canned yamato‐ni beef and found that they had an aroma that was significantly (P < 0.01) similar to the odor of 121°C‐heated beef than 100°C‐heated beef. The panel also noted that the aroma of 121°C‐heated beef tended to be (P < 0.1) preferable than that of 100°C‐heated beef. These results suggest that retort beef aroma is one constituent of palatability in canned beef. GC‐MS (gas chromatography‐mass spectrometry) analysis of the volatile fraction obtained from 100°C‐ and 121°C‐heated beef showed that the amounts of pyrazine, 2‐methylpyrazine and diacetyl were higher in the 121°C‐heated beef than in the 100°C‐heated beef. GC‐sniffing revealed that the odor quality of pyrazines was similar to that of retort beef aroma. Therefore, pyrazines were suggested to be a candidate responsible for the retort beef aroma. Analysis of commercial uncured corned beef and cured corned beef confirmed the presence of pyrazine, 2‐methylpyrazine and 2,6‐dimethylpyrazine.  相似文献   

Color development of proanthocyanidins in vanillin-hydrochloric acid reaction   总被引：1，自引：0，他引：1  

Tohru Mitsunaga  Takao Doi  Yuuki Kondo  Isao Abe 《Journal of Wood Science》1998,44(2):125-130

The influence of proanthocyanidin (PA) structures contained in bark on color development in the vanillin-hydrochloric acid (V-HCl) method used widely as a quantitative method for measuring PA were examined. The maximal absorption wavelength was different in terms of the bark from which the PA was obtained. Phenyl nucleus (resorcinol, phloroglucinol) constituting the A-ring of PA reacts with vanillin to produce the color. The maximal absorption wavelengths of the solutions from synthesized procyanidin and profisetinidin were 500 and 540 nm, respectively, indicating that the color tone differs in the V-HCl method based on the hydroxylation patterns of the A-ring. The colored solution of (+)-catechin with vanillin was dialyzed, and the resulting product (C-VC) was analyzed by gel permeation chromatography and ¹H nuclear magnetic resonance. It was found that C-VC was a polymer complex consisting of 9mol (+)-catechin moieties and 10mol vanillin moieties. It was presumed that the cationized vanillin molecules that do not combine with (+)-catechin play an important role on color development in the presence of C-VC.This study was presented at the 44th and 45th Annual Meetings of The Japan Wood Research Society, Nara and Tokyo, April 1994 and April 1995  相似文献   

Corrosive-wear characteristics of diamond-coated cemented carbide tools     

Takao Morita  Kaoru Banshoya  Takahiro Tsutsumoto  Yasuhide Murase 《Journal of Wood Science》1999,45(6):463-469

Continuous milling tests with diamond-coated and uncoated cemented carbide tools and polycrystalline diamond tools were carried out on air-dried and wet melapi (Shorea sp.) and western redcedar (Thuja plicata D. Don). These tools were examined for corrosive-wear characteristics of the tool-edge appearance, cutting-edge profile, edge recession, and cutting-power consumption. The tool surfaces were observed with a scanning electron microscope and were analyzed by energy-dispersive X-ray spectroscopy. Based on these examinations, the occurrence of corrosive wear while cutting wet woods was confirmed for the uncoated and polycrystalline diamond tools. In contrast, the coated tools did not exhibit corrosive wear, nor did delamitation or wear of the diamond film occur with any of the work materials. The diamond-coated tools showed high resistance not only to mechanical wear but also to corrosive wear.The results of this work were presented at the 48th annual meeting of the Japan Wood Research Society, Shizuoka, April 1998  相似文献   

Unique expression patterns of myosin heavy chain genes in the ductus arteriosus and uterus of rabbits.     

H Sakurai  S Imamura  Y Furutani  A Takao  K Momma  R Matsuoka 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(9):1049-1054

In smooth muscle tissue, two smooth muscle myosin heavy chain (MHC) isoforms (SM1, SM2) and two non-muscle MHC isoforms (NMA, NMB) have been identified. The purpose of our study was to clarify whether smooth muscle MHC mRNA expression reflects the physiological and functional state of the muscle. We studied the expression pattern of MHC mRNAs, using the S1-nuclease mapping procedure, in functionally and morphologically changeable organs; the ductus arteriosus (DA) during development (25 and 29 days of gestation, and from 3-day-old neonates) and uteri from virgin, day-10 pregnant (P10) and day-29 pregnant (P29) rabbits. The results demonstrated that SM2 expression was greater in the fetal DA than in the fetal aortic and pulmonary arteries, but that it decreased significantly following closure of DA. In the gravid uterus, SM1 expression was significantly (P<0.05) strong compared to other MHC mRNAs from virgin to P10 rabbits. During pregnancy, NMB expression showed a tendency to increase until P10, and after P10, SM2 expression increased dramatically and NMB expression decreased to give almost a mirror image of the SM2 expression. Smooth muscle type (SM1, SM2) was significantly (P<0.05) strong compared to non-muscle type expression (NMA, NMB) at P29. These data suggest that smooth muscle MHC mRNA, especially SM2 expression reflects the physiological and functional state of the smooth muscle.  相似文献   

Effect of salinity change and exposure time on the egg stages of two abalone species Haliotis discus discus and H. gigantea     

Manuel  Albert V.  Tu  Phan Thi Cam  Tsutsui  Naoaki  Yoshimatsu  Takao 《Fisheries Science》2020,86(1):27-33

Fisheries Science - The embryonic developmental response of two abalone species (disk abalone Haliotis discus discus, giant abalone Haliotis gigantea) to a drop in salinity with different exposure...  相似文献   

Impacts of short-term salinity and turbidity stress on the embryonic stage of red sea bream Pagrus major     

Phan  Thi Cam Tu  Manuel  Albert Valdish  Tsutsui  Naoaki  Yoshimatsu  Takao 《Fisheries Science》2020,86(1):119-125

Fisheries Science - Heavy rainfall can reduce salinity and increase turbidity in coastal waters, creating stressful conditions for the organisms found there, especially for the early stages of...  相似文献   

Complementary DNA cloning and functional analysis of lycopene β-cyclase in the brown alga Undaria pinnatifida     

Inoue  Akira  Iwayama  Toshiyuki  Ojima  Takao 《Fisheries Science》2019,85(4):717-729

Fisheries Science - To date, there is no functional evidence of the enzymes catalyzing carotenogenesis in brown algae. In this study, we have investigated the activity of brown algal lycopene...  相似文献