Seedborne fungi detected on stored solanaceous berry seeds and their biological activities     

Junji Nishikawa  Takao Kobayashi  Kazuto Shirata  Takashi Chibana  Keiko T. Natsuaki 《Journal of General Plant Pathology》2006,72(5):305-313

We isolated 629 fungi from 1296 berry seeds of solanaceous plants, including tomato (Lycopersicon esculentum), eggplant (Solanum melongena), bell pepper (Capsicum annuum), and red pepper (Capsicum annuum var. annuum) preserved for long and short terms. The isolates were classified into 22 genera excluding unidentified fungi, and the fungal floras were divided into two types: the tomato–eggplant and pepper groups. The results of cluster analysis with unweighted pair-group method with arithmetic average also supported these groups. Most tomato seeds infested with Geotrichum candidum germinated and grew the same as uninfested seeds. Cladosporium sphaerospermum and Arthrinium sp. isolated from eggplant seeds strongly suppressed germination, and Penicillium variabile suppressed seminal root elongation on eggplant. Alternaria alternata, Botrytis cinerea, and Myrothecium verrucaria detected from red pepper or bell pepper seeds were pathogenic to the fruits and the seedlings after artificial inoculation.  相似文献   

Occurrence of chrysanthemum virescence caused by “<Emphasis Type="Italic">Candidatus</Emphasis> Phytoplasma aurantifolia” in Okinawa     

Takashi Naito  Minoru Tanaka  Satoshi Taba  Tesuya Toyosato  Atsushi Oshiro  Kazuko Takaesu  Kazuo Hokama  Tomio Usugi  Shinji Kawano 《Journal of General Plant Pathology》2007,73(2):139-141

In 1999, a disease of chrysanthemum [Dendranthema grandiflorum (Ramat.) Kitamura], characterized by virescence of flowers, occurred in Okinawa Prefecture. The causal agent was identified as “Candidatus Phytoplasma aurantifolia” based on 16S rDNA sequencing. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB247462.  相似文献   

Diazepam potentiates the positive inotropic effects of histamine and forskolin in guinea-pig papillary muscles     

Hara  Kaigo  Minami  Watanabe  Gomi  Nishimura  Chugun  & Kondo 《Journal of veterinary pharmacology and therapeutics》1998,21(5):375-379

There have been diverse reports on the effects of diazepam on cardiac contractility. The purpose of this study was to examine whether diazepam modifies the inotropic response elicited by histamine on an isolated guinea-pig papillary muscle. The responses of electrically driven papillary muscle to histamine and cyclic AMP-related inotropic agents were recorded in the absence and in the presence of diazepam. Histamine and forskolin, which directly stimulate adenylate cyclase, significantly increased the contractile force in the papillary muscle in a concentration-dependent manner. A histaminergic H₂-receptor antagonist, cimetidine, but not a H₁-receptor antagonist, diphenhydramine, at 10 μ M produced a rightward shift in the concentration-response curve for histamine. Diazepam (10 μ M ) shifted the concentration-response curve for histamine and forskolin to the left by 1.8 and 1.6 times, respectively. Neither a central type (fulmazenil) nor a peripheral type (PK11195) of benzodiazepine receptor antagonist modified the effect of diazepam on the histaminergic-evoked contraction. Phosphodiesterase blockade by 3-isobutyl-1-methylxanthine shifted the concentration-dependent curve for histamine to the left. A combination of 3-isobutyl-1-methylxanthine also produced a leftward shift of the curve. However, there was no significant difference between the 3-isobutyl-1-methylxanthine only group and the combination group. These results indicate that diazepam potentiates the positive inotropic effect produced by histamine, probably mediated via an increase in cyclic AMP levels induced by histamine.  相似文献   

Therapeutic effects of electrolyte solution with oil emulsion on serious diarrhea in Holstein calves     

M Koiwa  A Hatsugaya  T Abe  S Minami 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(3):639-641

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Efficacy of naphthoquinones and imidocarb dipropionate on Theileria sergenti infections in splenectomized calves     

T Minami  T Nakano  S Shimizu  K Shimura  T Fujinaga  S Ito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(2):297-300

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Histological observations on the reproductive organs of harbor porpoises Phocoena phocoena incidentally caught in a set net installed off Usujiri, southern Hokkaido     

Yoshiharu  HONMA  Tatsuo  USHIKI  Hiroya  HASHIZUME  Masaei  TAKEDA  Takashi  MATSUISHI  Yoshiko  HONNO 《Fisheries Science》2004,70(1):94-99

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Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIα and IIβ genes of Japanese flounder Paralichthys olivaceus     

Prapansak  SRISAPOOME  Tsuyoshi  OHIRA  Ikuo  HIRONO  Takashi  AOKI 《Fisheries Science》2004,70(2):264-276

ABSTRACT:   The nucleotide sequences of Japanese flounder Paralichthys olivaceus , major histocompatibility complex (MHC) cDNA, classical MHC class Iα, non-classical MHC class Iβ, MHC class IIα and IIβ, were determined. The domain structures and antigen binding motifs of vertebrate MHC are conserved in the Japanese flounder MHC. A phylogenetic analysis supports the classification of these genes into class I and class II MHC. Classical MHC class Iα was ubiquitously expressed, whereas the non-classical MHC class Iβ was expressed mainly in lymphoid organs, gills, intestine and stomach. The MHC classes IIα and IIβ were also ubiquitously expressed.  相似文献   

Characteristics of the distribution of bacteria, heterotrophic nanoflagellates and ciliates in Hiroshima Bay in summer     

Takashi  KAMIYAMA  Satoshi  ARIMA  Mutsumi  TSUJINO 《Fisheries Science》2003,69(4):755-766

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Reaction behavior of lignin in supercritical methanol as studied with lignin model compounds   总被引：2，自引：0，他引：2  

E.?Minami  H.?Kawamoto  S.?SakaEmail author 《Journal of Wood Science》2003,49(2):158-165

 The reaction behavior and kinetics of lignin model compounds were studied in supercritical methanol with a batch-type supercritical biomass conversion system. Guaiacol, veratrole, 2,6-dimethoxyphenol, and 1,2,3-trimethoxybenzene were used as model compounds for aromatic rings in lignin. In addition, 5-5, β-1, β-O-4, and α-O-4 types of dimeric lignin model compounds were used as representatives of linkages in lignin. As a result, aromatic rings and 5-5 (biphenyl)-type structures were stable in supercritical methanol, and the β-1 linkage was not cleaved in the β-1-type structure but converted rapidly to stilbene. On the other hand, β-ether and α-ether linkages of β-O-4 and α-O-4 lignin model compounds were cleaved rapidly, and these compounds decomposed to some monomeric compounds. Phenolic compounds were found to be more reactive than nonphenolic compounds. These results indicate that cleavages of ether linkages mainly contribute to the depolymerization of lignin, whereas condensed linkages such as the 5-5 and β-1 types are not cleaved in supercritical methanol. Therefore, it is suggested that the supercritical methanol treatment effectively depolymerizes lignin into the lower-molecular-weight products as a methanol-soluble portion mainly by cleavage of the β-ether structure, which is the dominant linkage in lignin. Received: December 19, 2001 / Accepted: April 30, 2002 Acknowledgments This research has been done under the research program for the development of technologies for establishing an ecosystem based on recycling in rural villages for the twenty-first century from the Ministry of Agriculture, Forestry and Fisheries, Japan; by a Grant-in-Aid for Scientific Research (B)(2) (no.12460144, 2001.4–2003.3) from the Ministry of Education, Culture, Sports, Science and Technology, Japan; and under the research program from Kansai Research Foundation for Technology Promotion, Japan. The authors thank them for their financial support. This study was presented in part at the 45th Lignin Symposium, Ehime, Japan, October 2000 and the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, Japan, April 2002 Correspondence to:S. Saka  相似文献   

Seven-year changes in growth and crown shape of Thujopsis dolabrata var. hondai saplings after release from suppression     

Gaku Hitsuma  Takayuki Ota  Tatsuo Kanazashi  Takashi Masaki 《Journal of Forest Research》2006,11(5):387-387

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