Structure of the rotor of the V-Type Na+-ATPase from Enterococcus hirae     

Murata T  Yamato I  Kakinuma Y  Leslie AG  Walker JE 《Science (New York, N.Y.)》2005,308(5722):654-659

The membrane rotor ring from the vacuolar-type (V-type) sodium ion-pumping adenosine triphosphatase (Na+-ATPase) from Enterococcus hirae consists of 10 NtpK subunits, which are homologs of the 16-kilodalton and 8-kilodalton proteolipids found in other V-ATPases and in F1Fo- or F-ATPases, respectively. Each NtpK subunit has four transmembrane alpha helices, with a sodium ion bound between helices 2 and 4 at a site buried deeply in the membrane that includes the essential residue glutamate-139. This site is probably connected to the membrane surface by two half-channels in subunit NtpI, against which the ring rotates. Symmetry mismatch between the rotor and catalytic domains appears to be an intrinsic feature of both V- and F-ATPases.  相似文献   

Quantitative nested real-time PCR detection of <Emphasis Type="Italic">Verticillium longisporum</Emphasis> and <Emphasis Type="Italic">V. dahliae</Emphasis> in the soil of cabbage fields     

Shinpei Banno  Hidenari Saito  Hiroshi Sakai  Toshihiko Urushibara  Kentaro Ikeda  Takeshi Kabe  Isao Kemmochi  Makoto Fujimura 《Journal of General Plant Pathology》2011,77(5):282-291

Verticillium longisporum and V. dahliae, causal agents of Verticillium wilt, are spreading through the cabbage fields of Gunma Prefecture. Using the V. longisporum-specific intron within the 18S rDNA and differences between ITS 5.8S rDNA sequences in Japanese isolates of V. longisporum and V. dahliae, we developed three quantitative nested real-time (QNRT) PCR assays. The QNRT-PCR quantification of V. longisporum or V. dahliae in cabbage field soil was consistent with the severity of Verticillium wilt disease in those fields. In field trials of resistant cultivar YR Ranpo grown for three seasons in soil infested with the pathogen, disease severity and pathogen density in the soil were significantly reduced in a field moderately contaminated by V. dahliae, but only slightly reduced in a highly contaminated field. These results suggest that continuous cultivation of a resistant cultivar is an effective way to reduce the pathogen population. QNRT-PCR assays provide a powerful analytical tool to evaluate the soil population dynamics of V. longisporum and V. dahliae for disease management.  相似文献   

Effect of rapid oligotrophication by an aquatic treatment pilot plant on the microbial community of a mesotrophic Bog     

Takeshi Naganuma  Humitake Seki 《Water, air, and soil pollution》1988,42(3-4):387-396

Rapid oligotrophication by the operation of the ‘Bio-filter system’ has changed Doh-Hoh-Numa Bog from a moderate mesotrophic system to the oligotrophic end of such a system. However, the population growth rate of the bacterioplankton community was little affected, and continued to be controlled primarily by the DOC concentration even after the system operation. This DOC concentration was higher during the operation period, although environmental factors relevant to primary production were shifted to the direction of oligotrophication. The natural bacterioplankton community in the bog was shown to modify its physiological kinetics to maintain the growth rate at the optimal level, even when it was exposed to oligotrophication processes with the operation of the ‘Bio-filter system’.  相似文献   

Food-drug interaction between ferulic acid and nateglinide involving the fluorescein/H+ cotransport system   总被引：2，自引：0，他引：2  

Itagaki S  Kobayashi Y  Otsuka Y  Kubo S  Kobayashi M  Hirano T  Iseki K 《Journal of agricultural and food chemistry》2005,53(7):2499-2502

In clinical, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions involving transporters can often directly affect the therapeutic safety and efficacy of many drugs. However, there have been few studies on food-drug interactions involving transporters. Dietary polyphenols have been widely assumed to be beneficial to human health. Polyphenols are commercially prepared and used as functional foods. We report here for the first time that ferulic acid, which is widely used as a functional food, affects the transport of clinical agents. It is important to be aware of the potential of food-drug interactions and to act in order to prevent undesirable and harmful clinical consequences.  相似文献   

Growth, Net Photosynthetic Rate, Nutrient Status and Secondary Xylem Anatomical Characteristics of Fagus Crenata Seedlings Grown in Brown Forest Soil Acidified with H2SO4 Solution     

Takeshi Izuta  Taeko Yamaoka  Tatsuro Nakaji  Tetsushi Yonekura  Masaaki Yokoyama  Hideyuki Matsumura  Sachie Ishida  Kenichi Yazaki  Ryo Funada  Takayoshi Koike 《Water, air, and soil pollution》2001,130(1-4):1007-1012

Dry matter production, net photosynthetic rate, leaf nutrient status and trunk anatomical characteristics of Fagus crenata seedlings grown in brown forest soil acidified by adding H₂SO₄ solution were investigated. The soil acidification leaded to decreased (Ca+Mg+K)/Al molar ratio in the soil solution. Dry mass per plant of the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly reduced compared with the control value at 0 mg·L^?1. When net photosynthetic rate was reduced in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1, the carboxylation efficiency and maximum net photosynthetic rate at saturated CO₂-concentration were lower than the control values. The addition of H⁺ to the soil at 120 mg·L^?1iinduced a reduction in the concentration of Ca in the leaf. By contrast, the concentration of Al in the leaf was increased with increasing the amount of H⁺ added to the soil. The annual ring formed in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly narrower than that at 0 (control), 10, 30, 60 or 90 mg·L^?1. Based on the results obtained in the present study, we conclude that Fagus crenata is relatively sensitive to a reduction in the (Ca+Mg+K)/Al molar ratio of soil solution compared with Picea abies.  相似文献   

Effects of phosphate supply and elevated CO2 on root acid phosphatase activity in Pinus densiflora seedlings     

Mariko Norisada  Tetsuya Motoshige  Katsumi Kojima  Takeshi Tange 《植物养料与土壤学杂志》2006,169(2):274-279

  相似文献   

Plaque formation and replication of porcine parvovirus in embryonic swine kidney cell line, ESK cells     

H Kawamura  T Fujita  T Imada 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(3):803-808

  相似文献   

Enterotoxigenicity of heat-resistant and heat-sensitive strains of Clostridium perfringens     

H Sunagawa  T Tsuzuki  K Takeshi  Y Ando  S Oka 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(5):853-858

  相似文献   

Detection of uremic peaks in dogs by anion exchange high performance liquid chromatography.     

M Kawamura  F Ohashi  F Murakami  N Takai  R Nishimura  N Sasaki  A Takeuchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):951-955

Sera of dogs with gentamicin-induced uremia were analyzed by high performance liquid chromatography system with strongly basic macroreticular anion exchange resin. Satisfactory separation of peaks was achieved with good reproducibility after deproteinization of sera with trichloroacetic acid at a final concentration of 3%, confirming that the system was suitable for qualitative analysis of uremic serum. The chromatograms showed that the number of peaks and the peak area had relation to concentrations of serum urea nitrogen or creatinine and severity of uremia. Four peaks were selected as suspected canine uremic peaks with high correlation to serum creatinine concentrations which were hardly influenced by extrarenal factors. The results suggested that these four fractions might contain uremic toxins.  相似文献   

Pathogenicity and distribution of egg-drop syndrome-1976 virus (JPA-1) in inoculated laying hens     

S Yamaguchi  T Imada  H Kawamura  T Taniguchi  M Kawakami 《Avian diseases》1981,25(3):642-649

Rhode Island Red laying hens that lacked hemagglutination-inhibition antibody were inoculated orally with the JPA-1 strain of adenovirus isolated from a flock affected with egg-drop syndrome-1976 in Japan and observed for 80 days. Inoculated hens laid abnormal eggs, such as shell-less, soft-shelled, and cracked eggs and those with loss of pigmentation, from 8 days postinoculation (PI). Fifteen out of 16 hens laid abnormal eggs. Egg-production rte fell from 94% to 50% between 13 and 16 days PI. When the abnormal eggs were excluded, egg production was 17%; then it recoverd slowly, reaching 67% by the end of the experiment. The virus was recovered from various organs of inoculated hens from 3 to 7 days PI. Fluorescent antigens of the virus were observed in the epithelial cells and desquamated cells from the epithelium of the uterus and isthmus 10 and 14 days PI.  相似文献