Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance     

Zhi-Shan Lin  Zhi-Fu Cui  Xiang-Yan Zeng  You-Zhi Ma  Zeng-Yan Zhang  Toshiki Nakamura  Goro Ishikawa  Kazuhiro Nakamura  Hisashi Yoshida  Zhi-Yong Xin 《Euphytica》2007,158(1-2):109-118

Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452 were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably 2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines.  相似文献   

Mitochondrial DNA Restriction Fragment Length Polymorphisms in Malus Species     

S. Kato    S. Ishikawa    S. Imakawa    S. Komori    T. Mikami  Y. Shimamoto 《Plant Breeding》1993,111(2):162-165

Three apple (Malus×domestica) cultivars and 11 Malus accessions have been investigated by the probe hybridization method on their mitochondrial DNA (mtDNA). The gene probes used were: coxI, coxII, atpA, atp6, and atp9. Our results revealed enough variation to characterize ten mtDNA haplotypes among the Malus genotypes examined. The taxonomic and phylogenetic implications of mtDNA polymorphism are also discussed.  相似文献   

Evaluation of biological activities of extracts from the fruiting body of <Emphasis Type="Italic">Pleurotus citrinopileatus</Emphasis> for skin cosmetics     

Tian-Xiao Meng  Shoko Furuta  Sayaka Fukamizu  Ryoko Yamamoto  Hiroya Ishikawa  Enos Tangke Arung  Kuniyoshi Shimizu  Shoji Ohga  Ryuichiro Kondo 《Journal of Wood Science》2011,57(5):452-458

Pleurotus citrinopileatus Singer has recently become a popular delicacy in East Asian countries. We prepared a methanol extract, soluble fractions from the methanol extract, and a hot water extract of the fruiting bodies of P. citrinopileatus. The biological activities such as melanin biosynthesis inhibition, tyrosinase inhibition, antioxidant activities [1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC), and superoxide dismutase (SOD)-like activity], antibacterial activities, and antihyaluronidase activities of these extracts were evaluated. We found that the n-hexanesoluble, diethyl ether-soluble, and ethyl acetate-soluble fractions exhibited melanin biosynthesis inhibition in B16 melanoma cells, as well as antioxidant (ORAC) and antibacterial activities. However, the n-butanol-soluble and water-soluble fractions and the methanol and hot water extracts exhibited antioxidant (DPPH radical scavenging, SOD-like activity) and antihyaluronidase activities. These results indicate that the fruiting bodies of P. citrinopileatus have the potential to be used as an ingredient in skin cosmetics.  相似文献   

Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines     

Okumura S  Saitoh H  Ishikawa T  Wasano N  Yamashita S  Kusumoto K  Akao T  Mizuki E  Ohba M  Inouye K 《Journal of agricultural and food chemistry》2005,53(16):6313-6318

Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL.  相似文献   

Development of new DNA markers linked to the Fusarium wilt resistance locus Fom‐1 in melon     

T. Tezuka  K. Waki  M. Kuzuya  T. Ishikawa  Y. Takatsu  M. Miyagi 《Plant Breeding》2011,130(2):261-267

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Displacement above the hypocenter of the 2011 Tohoku-Oki earthquake   总被引：3，自引：0，他引：3  

Sato M  Ishikawa T  Ujihara N  Yoshida S  Fujita M  Mochizuki M  Asada A 《Science (New York, N.Y.)》2011,332(6036):1395

The moment magnitude (M(w)) = 9.0 2011 Tohoku-Oki mega-thrust earthquake occurred off the coast of northeastern Japan. Combining Global Positioning System (GPS) and acoustic data, we detected very large sea-floor movements associated with this event directly above the focal region. An area with more than 20 meters of horizontal displacement, that is, four times larger than those detected on land, stretches several tens of kilometers long along the trench; the largest amount reaches about 24 meters toward east-southeast just above the hypocenter. Furthermore, nearly 3 meters of vertical uplift occurred, contrary to observed terrestrial subsidence.  相似文献   

Magnetic properties of the lunar crystalline rock and fines     

Nagata T  Ishikawa Y  Kinoshita H  Kono M  Syono Y  Fisher RM 《Science (New York, N.Y.)》1970,167(3918):703-704

Magnetic measurements have shown that nondiamagnetic minerals in a lunar crystalline rock of type B are (free Fe(2)+ in paramagnetic pyroxenes) : (antiferromagnetic FeSiO(3)) : (antiferromagnetic FeTiO(3)) : (ferromagnetic iron) = 4.3 : 7 : 20 : 0.08 in weight percentage. The abundance of ferromagnetic Fe in the lunar fines is about 7.5 times its abundance in the crystalline rock. The natural remanent magnetization of the crystalline rock of 7.5 x 10(-6) emu/ g in intensity may not be attributable to its thermoremanent magnetization.  相似文献   

Collection,mapping, and annotation of over 28,000 cDNA clones from japonica rice   总被引：2，自引：0，他引：2  

Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team  Kikuchi S  Satoh K  Nagata T  Kawagashira N  Doi K  Kishimoto N  Yazaki J  Ishikawa M  Yamada H  Ooka H  Hotta I  Kojima K  Namiki T  Ohneda E  Yahagi W  Suzuki K  Li CJ  Ohtsuki K  Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group  Otomo Y  Murakami K  Iida Y  Sugano S  Fujimura T  Suzuki Y  Tsunoda Y  Kurosaki T  Kodama T  Masuda H  Kobayashi M  Xie Q  Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379

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Mechanisms of cadmium accumulation in rice grains and molecular breeding for its reduction     

Satoru Ishikawa 《Soil Science and Plant Nutrition》2020,66(1):28-33

ABSTRACT

Cadmium (Cd) has an important impact on agriculture, as the excessive consumption of this element from contaminated food crops leads to toxicity in humans. Rice is the greatest source of dietary Cd intake in populations consuming rice as a staple food. Therefore, reducing the Cd concentration of rice grains for diminishing the potential risk of Cd for human health is a major challenge. This review summarizes the main achievements on Cd accumulation in rice by our research group. Using a positron-emitting tracer imaging system, we succeeded to visualize differences in real-time Cd dynamics from roots to panicles in rice varieties with different Cd accumulation abilities. Several loci or genes responsible for Cd accumulation in rice were found through quantitative trait loci (QTLs) analysis. Using the QTL qCdp7 responsible for efficiently extracting Cd from paddy soils, practical rice varieties for Cd-phytoextraction were developed by DNA marker-assisted breeding. A rice variety named ‘Koshihikari Kan No.1? carrying a mutant allele of OsNramp5 for reducing Cd concentration in rice grains was produced by mutant breeding with ion-beams, and breeding programs have been implemented to transfer this allele into many Japanese rice varieties by DNA marker-assisted breeding. Growing low-Cd varieties aerobically would be a feasible way to simultaneously reduce inorganic arsenic and Cd concentrations in rice grains. These results provide basis for reducing Cd concentration in rice through breeding new varieties.  相似文献   

Genetic Erosion from Modern Varieties into Traditional Upland Rice Cultivars (Oryza sativa L.) in Northern Thailand     

R. Ishikawa  S. Yamanaka  Y. Fukuta  S. Chitrakon  C. Bounphanousay  K. Kanyavong  L.-H. Tang  I. Nakamura  T. Sato  Y.-I. Sato 《Genetic Resources and Crop Evolution》2006,53(2):245-252

The purpose of this study was to assess the extent of genetic erosion of traditional upland germplasm in northern Thailand as a result of gene-flow from distinct strains carrying different genotypes. Even modern variety specific markers have not been developed, there is a comparative population in Laos. Thus, both populations were compared with various characters to evaluate gene-flow from modern variety to landraces. Glutinous and glabrous strains are predominated in Laos. However, such strains were drastically decreased in north–east Thailand. Gene diversity is higher in Thailand, compared to Laos at seven isozyme loci. This was a result of the higher frequencies of Indica strains and heterozygotes in Thailand. Plastid type was also determined by using an INDEL marker. Nearly half of Indica strains carried the Japonica plastid. Heterozygotes also tended to carry Japonica cytoplasm. Such nuclear–cytoplasm substituted strains and heterozygotes were probably generated by natural hybridization. Japonica strains tended to be a maternal donor rather than Indica ones. Or Indica strains would easily release pollens, which grow outside of upland fields.  相似文献