Case of calcifying epithelial odontogenic tumour in a dog     

T. Ishikawa  H. Yamamoto 《The Journal of small animal practice》1996,37(12):597-599

A 12-year-old male shih tzu dog was diagnosed as having a calcifying epithelial odontogenic tumour. One and a half years prior to presentation, a mass was noticed on the right mandible by the owners. Radiography revealed irregular, faintly radiopaque material within the tumour. A right hemimandibulectomy was performed, based on the clinical diagnosis of osteosarcoma. Histopathological evaluation of the mandibular mass showed that it was composed of a polyhedral odontogenic epithelial cell nest, fibrous stroma, homogeneous eosinophilic material and rounded calcifying material. The eosinophilic material was visualised by staining with Congo red, and observed under green birefringence by polarisation microscopy. Although the tumour had not recurred 12 months after surgery, the dog was euthanased because of aspiratic pharyngitis. The literature on the clinical behaviour of calcifying epithelial odontogenic tumours in dogs and cats is reviewed.  相似文献   

Sex-chromosome chimerism (XX-XY) in heterosexual bovine triplets   总被引：3，自引：0，他引：3  

H Kanagawa  K Kawata  T Ishikawa  J Muramoto  H Ono 《The Japanese journal of veterinary research》1965,13(4):121-126

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The Possibility of Chromosome Classification as Identified by Trypsin-Giemsa Banding Patterns     

Y.-I. Miyake  T. Ishikawa 《Reproduction in domestic animals》1978,13(1):33-37

Inhalt Möglichkeit einer Chromosomenklassifikation durch eine Trypsin-Giemsa-Bänderungstechnik. Es wird eine modifizierte Trypsin-Giemsa-Bandtechnik für Chromosomen beschrieben, die beim Schwein eine deutliche Bänderung zur Chromosomenidentifikation ergibt. Die Bänderung wird erzielt durch eine Verkärzung der Kolchizineinwirkung auf 30 Minuten und eine Behandlung der 3–5 Tage alten Objektträger mit einer 0,1 % Trypsinlösung (Sörensenpuffer pH 6,8) über eine Dauer von 10–20 Minuten bei 4°C und anschlieβender Färbung mit 2 % Giemsalösung (Phosphatpuffer pH 6,8) für 7–10 Minuten. Mit dieser Technik konnten die 18 autosomalen Chromosomen und die beiden Geschlechtschromosomen des Schweines identifiziert werden. Einzelheiten der G-Bandzeichnung eines jeden Chromosoms dieser Spezies werden beschrieben und in einem Karyotypogramm photographisch und schematisch belegt. Das beschriebene Verfahren kann für Zytogenetiker der Human- und Tiermedizin von Bedeutung sein. Contents In the present paper the authors present a modified trypsin-giemsa chromosomal banding technique showing clear banding patterns for the identification of pig chromosomes. The banding patterns were induced by the reduction of colchicine density or trypsin treatment at a critical temperature of 4°C. Identification of 18 pairs of autosomes and a pair of sex-chromosomes of pig were made successfully. Details of the G-banding patterns of each chromosome were described and illustrated both in a karyotypical photograph and in a schematic diagram. The methods may be of importance to veterinary cytogenetics and genetical pathology, as well as to human medicine.  相似文献   

Osteosarcoma in the maxilla of a brown bear (Ursus arctos)     

E Momotani  H Aoki  Y Ishikawa  T Yoshino 《Veterinary pathology》1988,25(6):527-529

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Undifferentiated astrocytoma in a cow     

S Sato  Y Ishikawa  K Ohshima 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(4):597-600

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A fertile case of a bovine heterosexual twin female with sex-chromosomal chimerism     

Yoh-Ichi Miyake  Tsune Ishikawa  Tsuneo Abe  Masanori Komatsu  Yoshiaki Kodama 《Reproduction in domestic animals》1980,15(2):103-106

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Chromosome studies on heterosexual twins in cattle. II. Significance of sex-chromosome chimerism (XX-XY) in early diagnosis of freemartin   总被引：4，自引：0，他引：4  

H Kanagawa  K Kawata  T Ishikawa 《The Japanese journal of veterinary research》1965,13(2):43-49

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Attempt at in vitro maturation of minke whale (Balaenoptera Bonaerensis) oocytes using a portable CO2 incubator     

Iwayama H  Ishikawa H  Ohsumi S  Fukui Y 《The Journal of reproduction and development》2005,51(1):69-75

The present study was conducted to investigate whether a portable CO2 incubator was effective for in vitro maturation (IVM) of bovine, porcine and minke whale oocytes, and the effect of maturation media supplemented with different hormones; porcine follicle stimulating hormone (pFSH), estradiol-17beta (E2), or pregnant mare's serum gonadotropin (PMSG): human chorionic gonadotropin (hCG) for minke whale immature oocytes was also examined. In vitro maturation rates of bovine and porcine oocytes cultured in the portable CO2 incubator were not significantly different from the standard CO2 incubator. In minke whale IVM culture using the portable incubator, the maximum expansion of cumulus mass was observed by pFSH/E2 and PMSG/hCG at the end of IVM culture. Moreover, the IVM culture period was shortened to 28-30 h from 96-120 h previously reported. The proportion of matured oocytes cultured in the medium supplemented with pFSH/E2 (26.7%) was significantly higher (P<0.05) than that with PMSG/hCG (6.9%). The present study indicates that a portable CO2 incubator is a useful device for minke whale IVM culture on a research base ship, and the addition of pFSH/E2 into an IVM medium enhanced cumulus expansion and the proportion of minke whale matured oocytes.  相似文献   

Acute haemorrhagic leucoencephalitis in a Californian sea lion (Zalophus californianus)     

Shibahara T  Sato K  Kato M  Takeda A  Yokota T  Ishikawa Y  Kadota K 《The Veterinary record》2004,154(9):270-272

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Relationship between serum sex hormone concentrations and histology of seminiferous tubules of captured baleen whales in the Western North Pacific during the feeding season     

Watanabe H  Mogoe T  Asada M  Hayashi K  Fujise Y  Ishikawa H  Ohsumi S  Miyamoto A  Fukui Y 《The Journal of reproduction and development》2004,50(4):419-427

The present study was conducted to obtain new information on relationships among serum testosterone (T), estradiol-17 beta (E(2)), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) concentrations and histology of seminiferous tubules in captured common minke and Bryde's whales during the feeding season. Blood samples and testes were collected from common minke (n=39 for blood samples, n=15 for testes) and Bryde's (n=14 for blood samples, n=7 for testes) whales captured from May 2001 to August 2001 in the Western North Pacific. Serum T concentrations, in 35.9% of the common minke and 57.1% of Bryde's whales, were below the detection limit (< 2.5 pg/ml). There were no significant differences in the serum concentrations of E(2), FSH, and LH among immature, mature common minke and Bryde's whales except that LH levels of immature Bryde's whales was higher than those of common minke whales. In most seminiferous tubules of mature whales, only a single-layer of spermatogonia was observed. However, spermatozoa were observed in seminiferous tubules in 2/13 of mature common minke and 4/4 of mature Bryde's whales with the low or undetectable T levels. These results indicate that the low serum T concentrations reflect the inactivity of spermatogenesis in both baleen whales, and that it is not possible to assess gonadal activity in either common minke or Bryde's whales using serum sex hormone concentrations during the feeding season.  相似文献