Comparative studies on lipid analysis and ultrastructure in porcine and southern minke whale (Balaenoptera bonaerensis) oocytes     

Fujihira T  Kinoshita M  Sasaki M  Ohnishi M  Ishikawa H  Ohsumi S  Fukui Y 《The Journal of reproduction and development》2004,50(5):525-532

The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride, total cholesterol, phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales, and also in fresh and vitrified immature porcine oocytes, were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmed porcine oocytes. In particular, the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly (P<0.05) higher than those from prepubertal pigs. Furthermore, the distribution of lipid droplets in fresh and vitrified immature oocytes was observed in transmission electron microscopy. Lipid droplets in the prepubertal minke whale oocytes were distributed throughout the cytoplasm. In contrast, adult minke whales had larger lipid droplets which were distributed mainly in the central portion of the cytoplasm. The lipid droplets of immature oocytes from prepubertal pigs were larger than those in minke whale oocytes. These results indicated that the difference in the distribution of the cytoplasmic lipid droplets may result in the difference in the color tone of both prepubertal and adult whale oocyte cytoplasm.  相似文献   

The pattern of ovarian development in the prepubertal antarctic minke whale (Balaenoptera bonaerensis)     

Tetsuka M  Asada M  Mogoe T  Fukui Y  Ishikawa H  Ohsumi S 《The Journal of reproduction and development》2004,50(4):381-389

This study describes the morphological and morphometrical changes associated with prepubertal ovarian development in the Antarctic minke whale (Balaenoptera bonaerensis). Ovaries were harvested from 94 immature minke whales caught in the Antarctic Ocean during the summer feeding season (December-March). Notable differences in ovarian size and morphology were found among animals. Up to 10 folds difference in ovarian weight was found among prepubertal whales of similar body size. During the prepubertal period, ovaries grew slowly and approximately doubled their weight. The morphologies of right and left ovaries were almost identical while the growth of the ovary appears to occur preferentially on the right side. The most striking morphological feature was numerous small antral follicles less than 5 mm in diameter found in ovaries of younger immature whales. The occurrence of these ovaries was highest in whales less than 6 m long and gradually decreased as body length increased. In larger whales, the occurrence of ovaries with a smaller number of follicles up to 10 mm and thick tunica albuginea increased. Thus, the ovary of the Antarctic minke whale experiences bursts of small follicular development during the early prepubertal period before becoming a more developed ovary with fewer but larger follicles, and thick tunica albuginea.  相似文献   

Investigating phenotypic variability in Colletotrichum lindemuthianum populations     

Pinto JM  Pereira R  Mota SF  Ishikawa FH  Souza EA 《Phytopathology》2012,102(5):490-497

Colletotrichum lindemuthianum, causal agent of anthracnose in the common bean, has wide genetic variability. Differential bean cultivars and morphological and physiological characteristics were used to analyze 74 isolates of C. lindemuthianum collected in two counties in the state of Minas Gerais, Brazil. Six different races were found, with a predominance of race 65 at both locations. Isolates were classified according to their sensitivities to the fungicide thiophanate-methyl, normally used in the control of common bean anthracnose. In all, ≈10% of isolates were resistant to the fungicide in vitro. Characteristics such as indexes of mycelia growth rate, colony diameter, sporulation capacity, and percentage of germination demonstrated the high genetic variability of C. lindemuthianum. We also observed variation in conidial cytology. The conidia of most isolates showed septa formation after germination, in contrast to septa absence, previously reported in the literature. Sexual and asexual reproduction were evaluated for mechanisms that may contribute in the generation of variability in C. lindemuthianum. Conidial anastomosis tubes were commonly found, indicating that asexual reproduction can help increase variability in this species. Information from this study confirmed high variability in C. lindemuthianum and will guide future studies in basic knowledge and applied technologies.  相似文献   

First report of fig mosaic virus infecting common fig (Ficus carica) in Japan     

Kazuya Ishikawa  Kensaku Maejima  Susumu Nagashima  Nobuo Sawamura  Yusuke Takinami  Ken Komatsu  Masayoshi Hashimoto  Yasuyuki Yamaji  Jun Yamamoto  Shigetou Namba 《Journal of General Plant Pathology》2012,78(2):136-139

For the first time, fig mosaic virus (FMV) was detected in common fig (Ficus carica) trees in Shimane, Japan. These trees exhibited mosaic, ringspots, or distortion, accompanied by chlorosis on leaves and yellow spots on fruits. Some of the symptomatic trees were infested with the eriophyid mite Aceria ficus. The virus was detected based on RT-PCR, followed by sequencing. The amplified 300 base-pair fragments shared 83.5–91.5% identity with the corresponding region of RNA-dependent RNA polymerase gene of FMV isolates previously reported in Turkey, Iran, and Italy.  相似文献   

Effects of complete submergence on seedling growth and survival of five riparian tree species in the warm-temperate regions of Japan     

Motoki Higa  Toshinobu Moriyama  Shingo Ishikawa 《Journal of Forest Research》2012,17(2):129-136

To reveal the relationship between tolerance to submergence and the distribution ranges of riparian tree species in the floodplains of the warm-temperate regions in Japan, we assessed the response and tolerance of current-year seedlings of two Salix and three Ulmaceae species to submergence treatments for 1, 2, 4, and 8 weeks. We investigated shoot elongation, number of leaves, and survival rate of seedlings during the experiment, and biomass allocation was determined at the end of the experiment. Shoot elongation and leaf production of seedlings were suppressed in all the species during the submergence treatments. However, the seedlings of Salix chaenomeloides and S. pierotii elongated shoots rapidly and produced leaves after the treatments. All the underground biomass in these two species was larger than aboveground biomass. These results are considered as typical responses of flood-tolerant species. In contrast, the underground biomass of Ulmaceae seedlings (Celtis sinensis var. japonica, Aphananthe aspera, and Ulmus parvifolia) decreased more rapidly than aboveground biomass during prolonged submergence. Furthermore, A. aspera seedlings also showed suppression of shoot elongation and leaf production after the treatments. Our results indicate that the three Ulmaceae species are less tolerant to submergence than the two Salix species. In conclusion, we suggest that submergence stress is one of the important factors determining the distribution of tree species in the floodplains of lowlands in the warm-temperate regions of Japan.  相似文献   

Fine mapping of quantitative trait loci affecting organ weights by mouse intersubspecific subcongenic strain analysis     

Md. Bazlur Rahman Mollah  Akira Ishikawa 《Animal Science Journal》2013,84(4):296-302

The genetic architecture of organ weights is not well understood. In this study, we fine‐mapped quantitative trait loci (QTLs) affecting organ weights by characterizing six intersubspecific subcongenic mouse strains with overlapping and non‐overlapping genomic regions on chromosome 2 derived from wild Mus musculus castaneus. QTLs for heart, lung, spleen and kidney weights were revealed on a 6.38‐Mb genomic region between two microsatellite markers, D2Mit323 and D2Mit472. Effects of the castaneus alleles at the organ weight QTLs were all opposite in direction to a body weight QTL previously mapped to the same genomic region. In addition, new QTLs for lung and kidney weights were revealed on a different 3.57‐Mb region between D2Mit205 and D2Mit182. Their effects were dependent on that of another body weight QTL previously mapped to that genomic region. The organ weight QTLs revealed were all duplicated in independent analyses with F₂ intercross populations between subcongenic strains carrying these QTLs and their background strain. The findings suggested that organ weights are not exclusively regulated by genetic loci that commonly influence overall body weight and rather that there are loci contributing to the growth of specific organs only.  相似文献   

Cloning and characterization of porcine CArG binding factor A expression in the anterior pituitary     

Takahashi J  Ishikawa A  Susa T  Kato T  Kato Y 《The Journal of reproduction and development》2008,54(6):424-430

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Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes          下载免费PDF全文

Wakana Izumi  Yuko Takuma  Ryo Ebihara  Wataru Mizunoya  Ryuichi Tatsumi  Mako Nakamura 《Animal Science Journal》2018,89(8):1214-1219

Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.  相似文献   

Spontaneous osteosarcoma in a rabbit ( Oryctolagus cuniculus)     

Kondo H  Ishikawa M  Maeda H  Onuma M  Masuda M  Shibuya H  Koie H  Sato T 《Veterinary pathology》2007,44(5):691-694

A 6-year-old male cross-breed rabbit ( Oryctolagus cuniculus) was presented with lameness and severe swelling from the right shoulder to brachium. On 16-detector helical computed tomography images of the amputated right forelimb after being fixed in formalin, evident proliferative and destructive lesions of bone were observed. On histologic examination, the tumor was composed of proliferating neoplastic cells that resembled histiocytes, with abundant osteoid production. A large number of multinucleated giant cells were found throughout. This case was diagnosed as osteosarcoma by clinical, radiographic, and histologic findings. This is a rare case report of osteosarcoma in a rabbit consistent with canine predilection sites.  相似文献   

Direct connection of supercritical fluid extraction and supercritical fluid chromatography as a rapid quantitative method for capsaicinoids in placentas of Capsicum.   总被引：2，自引：0，他引：2  

K Sato  S S Sasaki  Y Goda  T Yamada  O Nunomura  K Ishikawa  T Maitani 《Journal of agricultural and food chemistry》1999,47(11):4665-4668

The fruits of Capsicum annuum L. are used worldwide as chili peppers and in folk medicines. The pungent components of C. annuum, which are irritants, are called capsaicinoids (CAPS), and the most abundant components are capsaicin, dihydrocapsaicin, and nordihydrocapsaicin. To analyze CAPS in the placentas of Capsicum fruits rapidly and safely, we used a directly connected system of supercritical fluid extraction and supercritical fluid chromatography (SFE/SFC). As a column for SFE/SFC, only a silica-type column was found to be suitable. The CAPS contents in placentas of C. annuum cv. Jalapeno (hot type) and C. annuum cv. Shishitoh (less-hot type) determined by the SFE/SFC method agreed well with those in the range of 0-13.81 mg g(-1) fr. wt determined by the usual extraction-HPLC method. The SFE/SFC method has the advantages of no need for pretreatment and no (or minimal) need for organic solvents. We conclude that this method is useful as a rapid (20 min) and safe screening test for the pungency of various Capsicum fruits.  相似文献