Evaluation of LHP? (1% hydrogen peroxide) cream versus petrolatum and untreated controls in open wounds in healthy horses: a randomized,blinded control study     

Tamás Tóth  Hans Brostr?m  Viveca B?verud  Ulf Emanuelson  Elisabeth Bagge  Tommy Karlsson  Kerstin Bergvall 《Acta veterinaria Scandinavica》2011,53(1):45

Background

Treatment and protection of wounds in horses can be challenging; protecting bandages may be difficult to apply on the proximal extremities and the body. Unprotected wounds carry an increased risk of bacterial contamination and subsequent infection which can lead to delayed wound healing. Topical treatment with antimicrobials is one possibility to prevent bacterial colonization or infection, but the frequent use of antimicrobials ultimately leads to development of bacterial resistance which is an increasing concern in both human and veterinary medicine.

Methods

Standardized wounds were created in 10 Standardbred mares. Three wounds were made in each horse. Two wounds were randomly treated with LHP^® or petrolatum and the third wound served as untreated control. All wounds were assessed daily until complete epithelization. Protocol data were recorded on day 2, 6, 11, 16, 21 and 28. Data included clinical scores for inflammation and healing, photoplanimetry for calculating wound areas and swab cytology to assess bacterial colonization and inflammation. Bacterial cultures were obtained on day 2, 6 and 16.

Results

Mean time to complete healing for LHP^® treated wounds was 32 days (95%CI = 26.9-37.7). Mean time to complete healing for petrolatum and untreated control wounds were 41.6 days (95%CI = 36.2-47.0) and 44.0 days (95%CI = 38.6-49.4) respectively. Wound healing occurred significantly faster in LHP^® wounds compared to both petrolatum (p = 0.0004) and untreated controls (p < 0.0001). There was no significant difference in time for healing between petrolatum and untreated controls. Total scores for bacteria and neutrophils were significantly (p < 0.0001) lower for LHP^® treated wounds compared to petrolatum from day 16 and onwards. Staphylococcus aureus and Streptococcus zooepidemicus were only found in cultures from petrolatum treated wounds and untreated controls.

Conclusions

Treatment with LHP^® reduced bacterial colonization and was associated with earlier complete wound healing. LHP^® cream appears to be safe and effective for topical wound treatment or wound protection.  相似文献   

Emergence and establishment of Usutu virus infection in wild and captive avian species in and around Zurich, Switzerland--genomic and pathologic comparison to other central European outbreaks     

Steinmetz HW  Bakonyi T  Weissenböck H  Hatt JM  Eulenberger U  Robert N  Hoop R  Nowotny N 《Veterinary microbiology》2011,148(2-4):207-212

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Comparative study of the effect of incorporated individual wheat storage proteins on mixing properties of rice and wheat doughs     

Oszvald M  Balázs G  Tömösközi S  Békés F  Tamás L 《Journal of agricultural and food chemistry》2011,59(17):9664-9672

The aim of this work was to compare the effects of incorporated wheat storage proteins on the functional properties of rice and wheat flours. The advantage of rice as a base flour compared to wheat is that it does not contain any wheat flour components and, therefore, has no interactive effect between wheat glutenin proteins. The incorporation of individual HMW glutenin subunit proteins (Bx6, Bx7, and By8) in different ratios had significant positive effects on the mixing requirements of both rice and wheat doughs. Reconstitution experiments using two x+y type HMW-GS pairs together with a bacterially expressed LMW-GS have been also carried out in this study. The largest effects of polymer formation and mixing properties of rice flour dough were observed when Bx and By subunits were used in a 1:1 ratio and HMW and LMW glutenin subunits in a 1:3 ratio. However, using the same subunit ratios in wheat as the base flour, these synergistic effects were not observed.  相似文献   

Precision Weed Detection using Terrestrial Laser Scanning Techniques     

Péter Riczu  Attila Nagy  Éva Lehoczky  János Tamás 《Communications in Soil Science and Plant Analysis》2015,46(4):309-316

Weeds compete with cultivated plants for nutrients and water in agricultural fields and orchards. Site-specific precision plant protection requires optimal plant-specific pesticide combinations and quantities. The first step in precision weed control is to map and detect weeds. Active and passive remote-sensing tools are available for weed detection. This article presents a study of precision weed detection using three-dimensional terrestrial laser scanning in an apple (Malus domestica) orchard. The laser scanner was able to identify two monocotyledonous (Digitaria sanguinalis, Echinochloa crus-galli) and two dicotyledonous (Galinsoga parviflora, Portulaca oleracea) weed species. In addition to weed identification, weed coverage was also determined using three algorithms. The results indicate that laser scanning has the potential for fast, accurate weed detection and could support the development of a plant-specific, selective, precision weed-control system that reduces water and pesticide use in orchards.  相似文献   

Beta-arrestin 2 mediates endocytosis of type III TGF-beta receptor and down-regulation of its signaling     

Chen W  Kirkbride KC  How T  Nelson CD  Mo J  Frederick JP  Wang XF  Lefkowitz RJ  Blobe GC 《Science (New York, N.Y.)》2003,301(5638):1394-1397

beta-Arrestins bind to activated seven transmembrane-spanning (7TMS) receptors (G protein-coupled receptors) after the receptors are phosphorylated by G protein-coupled receptor kinases (GRKs), thereby regulating their signaling and internalization. Here, we demonstrate an unexpected and analogous role of beta-arrestin 2 (betaarr2) for the single transmembrane-spanning type III transforming growth factor-beta (TGF-beta) receptor (TbetaRIII, also referred to as betaglycan). Binding of betaarr2 to TbetaRIII was also triggered by phosphorylation of the receptor on its cytoplasmic domain (likely at threonine 841). However, such phosphorylation was mediated by the type II TGF-beta receptor (TbetaRII), which is itself a kinase, rather than by a GRK. Association with betaarr2 led to internalization of both receptors and down-regulation of TGF-beta signaling. Thus, the regulatory actions of beta-arrestins are broader than previously appreciated, extending to the TGF-beta receptor family as well.  相似文献   

Impact of Aluminum on Phosphate Uptake and Acid Phosphatase Activity in Root Tips of Lotus Japonicus     

Veronika Zelinová  Jana Huttová  Igor Mistrík  Peter Pal'ove-Balang  Ladislav Tamás 《Journal of plant nutrition》2013,36(10):1633-1641

The effect of aluminum (Al) on phosphate homeostasis and induction of acid phosphatase activity and isoenzymes was analyzed in root tips of Lotus japonicus. The rapid uptake of phosphate as well as the decrease of soluble inorganic phosphate in tissues suggested, that the presence of Al in root apoplast causes precipitation of the most of absorbed phosphate. Acid phosphatase (ACP) isoenzymes are affected differentially during low pH and Al stress and both Al induced increases and decreases of ACP isoenzyme activities were observed.  相似文献   

Histological changes in insulin-immunoreactive pancreatic β-cells,and suppression of insulin secretion and somatotrope activity in brook trout (Salvelinus fontinalis) maintained on reduced food intake or exposed to acidic environment     

D. B. Mackett  W. H. Tam  J. N. Fryer 《Fish physiology and biochemistry》1992,10(3):229-243

Immature brook trout (Salvelinus fontinalis) were randomly divided into a pH control, a pH and food control and an acid-stressed group. Fish in the first two groups were held at neutral pH and those in the last group were maintained at pH 4.2 for up to two months. The food supply to the pH and food control group was restricted to simulate the reduction in food intake demonstrated for acid-stressed trout. Plasma insulin levels were significantly decreased from 5–20 ng/ml to 1–2 ng/ml and plasma cortisol levels were significantly increased from 5–10 ng/ml to as high as 70 ng/ml in the acid-stressed brook trout. Concomitantly, a significant decrease of 21–39% in the proportion (volume density) of insulin immunoreactive -cells was observed within the principal pancreatic islets. Somatic growth was stunted and ultrastructural morphometry revealed the suppression of somatotrope secretory activity in the acid-stressed fish. Restriction of food supply induced a smaller but still significant decrease in circulating levels of insulin which was however not accompanied by a reduction in insulin immunoreactive -cells. The rise in plasma cortisol levels was not significant, and the plasma levels of glucose and protein were unaffected. Nevertheless, somatotrope secretory activity was suppressed and somatic growth was stunted. This study demonstrates for the first time the complexity of the endocrine response to acid stress and that some of the response to acid stress can be attributed to the lowering of food intake.  相似文献   

Changes of condensed tannins during decomposition of leaves of Kandelia obovata in a subtropical mangrove swamp in China     

Hai-Chao Zhou  Nora Fung-yee Tam  Yi-Ming Lin  Shu-Dong Wei  Yuan-Yue Li 《Soil biology & biochemistry》2012,44(1):113-121

Kandelia obovata, with abundant condensed tannins (CTs), is a typical mangrove species in China, but little is known about the chemical alterations and ecological roles of CTs during leaf litter decomposition. A litterbag experiment was conducted to investigate the changes of CTs in a subtropical mangrove swamp along Zhangjiang River Estuary, China, using the colorimetric assays, reversed/normal-phase HPLC–ESI-MS and MALDI–TOF-MS techniques. Total phenolics (TP), extractable CTs (ECT) and total CTs (TCT) decreased rapidly, while bound CTs (BCT), including protein- and fibre-bound CTs in leaves, increased during decomposition, and these temporal changes were well-expressed by exponential functions. Negative correlations between nitrogen (N) and TP, as well as N and ECT were found; however, a positive correlation between N and BCT was detected, suggesting that CTs played an important role in humification during N immobilization. The HPLC–ESI-MS analyses showed that the polymerization degree of CTs had an initial increase, due to leaching, followed by a decrease in the subsequent shift towards abiotic or/and biotic degradation. MALDI–TOF-MS confirmed the degradation processes for CTs. A decrease in the degree of hydroxylation, along with an increase in glycosylation-CTs, was obtained during litter decomposition. These chemical changes enhanced the current knowledge on the potential ecological role of N transformation in CTs in mangrove swamps.  相似文献   

Estrus response and fertility after a single cloprostenol treatment in dairy cows with various ovarian structures     

Csilla Hatvani  Orsolya G. Balogh  Tamás Endr?di  Zsolt Abonyi-Tóth  István Holló  John P. Kastelic  Gy?rgy Gábor 《Canadian journal of veterinary research》2013,77(3):218-220

The objective of this study was to determine rates of estrus and conception in lactating multiparous Holstein cows given 500 μg of cloprostenol intramuscularly after detection of the following ≥ 60 d after parturition: a solid corpus luteum (CL), a CL with a nonechodense cavity ≤ 20 mm in diameter (CLcav), a luteal cyst (cavity > 20 mm in diameter and a luteinized wall > 3 mm in diameter), or a follicular cyst (cavity > 20 mm and a luteinized wall ≤ 3 mm in diameter). The estrus rates were 335/419 (80.0%), 183/223 (82.1%), 170/182 (93.4%), and 44/87 (50.6%), respectively (P < 0.0001), and the conception rates 30 to 36 d after insemination among the estrous cows with an apparently normal mucus discharge were 130/285 (45.6%), 44/141 (31.2%), 39/79 (49.4%), and 19/30 (63.3%), respectively (P < 0.002). Compared with a solid CL, a CLcav did not affect the estrus rate but significantly reduced the conception rate (P < 0.05), and the estrus rates were significantly higher and lower in cows with a luteal or follicular cyst, respectively (P < 0.05).  相似文献   

Comparison of Ethylene Glycol and Propylene Glycol for the Vitrification of Immature Porcine Oocytes     

Tamás SOMFAI  Michiko NAKAI  Fuminori TANIHARA  Junko NOGUCHI  Hiroyuki KANEKO  Naomi KASHIWAZAKI  István EGERSZEGI  Takashi NAGAI  Kazuhiro KIKUCHI 《The Journal of reproduction and development》2013,59(4):378-384

Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rate of vitrified oocytes in 35% PG (73.9%) was higher (P<0.05) than that in 35% EG (27.8%). Oocyte maturation rates did not differ among vitrified and non-vitrified control groups. Blastocyst formation in the vitrified EG group (10.8%) was higher (P<0.05) than that in the vitrified PG group (2.0%) but was lower than that in the control group (25.0%). Treatment of oocytes with 35% of each cryoprotectant without vitrification revealed a higher toxicity of PG on subsequent blastocyst development compared with EG. The combination of EG and PG resulted in 42.6% survival after vitrification. The maturation and fertilization rates of the surviving oocytes were similar in the vitrified, control and toxicity control (TC; treated with EG+PG combination without cooling) groups. Blastocyst development in the vitrified group was lower (P<0.05) than that in the control and TC groups, which in turn had similar development rates (10.7%, 18.1% and 23.3%, respectively). In conclusion, 35% PG enabled a higher oocyte survival rate after vitrification compared with 35% EG. However, PG was greatly toxic to oocytes. The combination of 17.5% EG and 17.5% PG yielded reasonable survival rates without toxic effects on embryo development.  相似文献