Assessment of genomic diversity of wild and cultivated tomato through Resistance Gene Analogue Polymorphism and I2 homologues     

Sanjukta Dey  Kaushik Ghose  Gaurab Gangopadhyay  Debabrata Basu 《Euphytica》2007,154(1-2):219-230

Plant disease resistance gene analogue polymorphism (RGAP) in tomato has been studied on the basis of NBS–LRR (Nucleotide Binding Site–Leucine Rich Repeat) class of genes using degenerated primers. Results revealed variable degree of polymorphism of the Group II compared to the Group I class of R genes. The probable explanation could be further diversification of the Group II class within tomato genotypes compared to the Group I class, which is relatively conserved. Furthermore, investigation of the diversity of the I2 class of genes, responsible for resistance to Fusarium oxysporum f.sp. lycopersici, revealed that genomic PCR mediated identification of functional I2 using the specific primers designed from the conserved domain of LRR region of I2 class of genes, is not possible on the basis of amplified fragment size. On the contrary, PCR amplification of 249 bp fragment from the conserved LRR region of I2 homologue I2C1/C2 gene from all the resistant lines indicated co-segregation of I2C1/C2 to the susceptible background along with the I2 gene during introgression. This may be an indirect PCR-mediated approach for marker-assisted breeding of functional allele of I2.  相似文献   

Resistance to Ascochyta blight in chickpea - Genetic basis     

S. K. Dey  Gurdip Singh 《Euphytica》1993,68(1-2):147-153

Summary Genetic regulation of host resistance in chickpea-Ascochyta rabiei interaction system is governed by two dominant complementary genes each in the genotypes GLG 84038 and GL 84099, whereas the resistance in a black seeded genotype ICC 1468 was controlled by one dominant and one recessive independent gene. In all the genotypes, resistance is operated by inter-allelic interactions. The genes conferring resistance in GLG 84038 were found to be different to those operating in GL 84099 and ICC 1468. Among the five dominant genes dispersed in 3 genotypes under study, at least one has been reported for the first time, as to date, only three dominant genes have been reported in the literature.The four identified dominant genes in GLG 84038 and GL 84099 have been named as Arc₁, Arc₂ (in GLG 84038) and Arc₃, Arc₄ (in GL 84099). The undistinguished dominant gene in ICC 1468 has been named as Arc_5(3,4) as it could not be equated or differentiated from Arc₃ or Arc₄. The recessive gene in ICC 1468 has been named as Arc₁.Generation mean analysis of the 6 resistant × susceptible crosses involving the same genotypes, revealed that the genes conferring resistance in any of the 3 genotypes did not follow simple Mendelian inheritance but were influenced by inter allelic interactions. Additive gene effect along with dominance were operative in all the 3 genotypes under study in conferring resistance. However, the mechanism of resistance in GLG 84038 and GL 84099 were primarily additive in nature while that in ICC 1468, dominance as well as dominance × dominance interactions were more important than additive gene action.  相似文献   

Effect of Crude Polyporin on Seed Germination and Root Growth; A Preliminary Study     

Bose SR  Bose AB  Dey KL 《Science (New York, N.Y.)》1948,107(2768):63-64

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小麦种子中与抗赤霉病性相关的生化成分分析   总被引：1，自引：0，他引：1  

林玲  陈怀谷  MICHAEL Trevan  PRAKASH M.Dey  王裕中  史建荣 《江苏农业学报》2003,19(1):23-27

用FPLC方法分析了抗小麦赤霉病程度不同的8个小麦品种种子的水溶性组分。结果表明,所有小麦品种都有1个保留时间为9min的吸收峰(P9),且抗病品种中该吸收峰的高度明显高于感病品种。种子水溶液经过氧化氢温育处理后,P9明显升高,表明有大量的P9生成。因此,推测P9可能是1种与小麦抗赤霉病性相关的生化成分,在小麦抗赤霉病的过程中起作用。  相似文献   

Pharmacokinetics of clindamycin HCl administered intravenously, intramuscularly and subcutaneously to dogs     

Lavy  Ziv  Shem-Tov  Glickman  & Dey 《Journal of veterinary pharmacology and therapeutics》1999,22(4):261-265

A buffered aqueous solution of clindamycin Hcl (200 mg/mL) was injected intravenously (i.v.) intramuscularly (i.m.) and subcutaneously (s.c.) in a non-randomized, partial cross-over trial involving six male and six female dogs. Blood samples were collected at conventional, predetermined time periods and serum drug concentrations were determined by microbiological assay. Dogs were observed clinically for signs of pain, and activity of serum creatine phosphokinase (CPK) was monitored after i.m. dosing. The i.v. data from five of the dogs best fitted a two-compartment open-system pharmacokinetic model whereas a non-compartment model was most suitable for analysis of the data from the remaining seven dogs. The mean i.v. elimination half-life (t1/2 beta) and the mean residence time (MRT) were 124 and 143 min, respectively. The mean volume of distribution at steady state (Vss) was 0.86 L/kg. Little pain was recorded upon i.m. injection; mean peak serum drug concentration (Cmax) was 4.4 micrograms/mL, the elimination half-life (t1/2el) was 247 min and the calculated bioavailability (F) was 115% of the i.v. dose. Serum CPK activity was elevated to 25-fold the pretreatment level in samples collected 4, 8 and 12 h after i.m. injection. Pain was not recorded after s.c. drug administration; the mean Cmax of 20.8 micrograms/mL was significantly greater than the corresponding value for the i.m. route, and F was 310%. The s.c. route appears to be superior to the i.m. route in terms of local tolerance and serum drug level; a 10 mg/kg SID treatment regimen is suggested for treatment of canine infections due to clindamycin sensitive bacteria.  相似文献   

Molecular characterization and induced expression analysis of the terminal complement component C9 in rohu,Labeo rohita     

Amruta Mohapatra  Sweta Das  Sudeshna Dey  Pramoda K. Sahoo 《Aquaculture Research》2020,51(4):1415-1427

The complement component 9 (C9) plays a significant role in the formation of membrane attack complex (MAC) on the targeted cell surface. The current study is dealt with molecular characterization of C9 gene from rohu, Labeo rohita, an important cultured carp species in India. An open reading frame (ORF) of 1998 bp was amplified by polymerase chain reaction (PCR) that encodes a polypeptide of 666 amino acids having a signal peptide of 19 amino acids and a mature peptide of 647 amino acids. The SMART domain architecture analysis revealed two thrombospondin type‐1 domains (TSP1), a low‐density lipoprotein receptor domain class A (LDLa), a membrane attack complex and perforin (MACPF) domain, and an epidermal growth factor (EGF)‐like domain. Multiple sequence alignment and evolutionary analysis revealed a primitive C9 sequence of rohu with maximum similarity and clustering with common carp, grass carp and zebrafish. C9 was highly expressed in liver and constitutively expressed in wide array of tissues except in eye of rohu juveniles. It was expressed during early developmental days of rohu including in milt. A variable level of up‐regulation in C9 expression was noticed upon poly I:C induction, Aeromonas hydrophila and Argulus siamensis infections in liver, spleen and gill tissues of rohu at different time points. A constitutive expression of C9 in different stages of rohu during the ontogeny and in response to pathogen exposures along with high degree of sequence homology with other fish species proved it as an important primitive immune molecule of the complement system lytic pathway.  相似文献