Antigenic analysis of Japanese encephalitis virus isolated in Hokkaido with monoclonal antibodies     

K Ochiai  I Takashima  N Hashimoto 《The Japanese journal of veterinary research》1989,37(1):21-26

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Ocular pathology of caprine beta-mannosidosis     

J A Render  K L Lovell  M Z Jones  C A Wheeler 《Veterinary pathology》1989,26(5):444-446

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Cellular injury and lipid peroxidation induced by hexavalent chromium in isolated rat hepatocytes   总被引：1，自引：0，他引：1  

S Ueno  N Susa  Y Furukawa  K Aikawa  I Itagaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(1):137-145

In order to elucidate the relationship between cellular injury and lipid peroxidation induced by hexavalent chromium (CrVI), isolated rat hepatocytes treated with any one of scavengers of active oxygen species, antioxidants or antichromium agent were incubated with K2Cr2O7 as CrVI (1 mM Cr). After the incubation, the development of lipid peroxidation was determined as thiobarbituric acid (TBA)-reacting materials in total lipid extracts from the incubated hepatocytes. Cellular injury was observed as a leakage of lactate dehydrogenase (LDH) from hepatocytes into incubation medium. The contents of reduced glutathione (GSH) in hepatocytes were also assessed. Results obtained were as follows: (1) CrVI facilitated lipid peroxidation in isolated hepatocytes after 20 min of incubation. On the other hand, the cellular injury induced by CrVI was barely observed even after 60 min of incubation. (2) The CrVI-induced lipid peroxidation was inhibited by catalase and mannitol as scavengers of active oxygen species, or N,N'-diphenyl-p-phenylenediamine and alpha-tocopherol as antioxidants. However the cytotoxicity of CrVI could not be prevented by these chemicals. (3) CrVI depleted the contents of intracellular GSH and diminished the activities of glutathione reductase (GR) and glutathione-S-transferase (GST) except glutathione peroxidase. (4) The scavengers of active oxygen species and the antioxidants could not prevent the depletion of intracellular GSH induced by CrVI. (6) Ascorbic acid, antichromium agent, prevented all of the lipid peroxidation, the cellular injury and intracellular GSH depletion induced by CrVI.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

A hemolytic assay for the measurement of equine complement     

K J Reis 《Veterinary immunology and immunopathology》1989,23(1-2):129-137

A hemolytic assay was developed for the measurement of functional equine complement activity. The assay utilizes antibody sensitized chicken erythrocytes as the target cell and was specific for classical pathway (antibody dependent) complement activity. The assay was found to be reproducible and more sensitive than previous reports using other species of target cells. Total serum complement (CH50) values were determined for five mares and their foals and followed over a period of 3 months.  相似文献   

Sind die Bitterstoffe Schutzmittel gegen Tierfraß?     

K. Friederichs 《Journal of pest science》1926,2(11):151-154

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Geheimrat Dr. Wappes — 80 Jahre alt     

Dr. Künkele 《European Journal of Forest Research》1940,62(1):22-24

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Regulation of Capacitation of Canine Spermatozoa during Co-culture with Heterologous Oviductal Epithelial Cells   总被引：2，自引：0，他引：2  

AM Petrunkina  K Simon  A-R Günzel-Apel  E Töpfer-Petersen 《Reproduction in domestic animals》2003,38(6):455-463

Progress of essential steps of the capacitation is coordinated in the oviductal isthmus, where sperm are stored in close contact with the epithelium. A crucial capacitational event is the phosphorylation of sperm membrane proteins. Regulation of the tyrosine phosphorylation by the oviduct has not been examined in dog sperm yet. The aim of this work was to study the effect of dog sperm binding to porcine oviductal epithelium on capacitation‐induced cellular and molecular changes. Epithelial cells were stripped from the oviducts of post‐puberal sows and cultured for 5–7 days at 39°C and 5% CO₂ on Biomatrix‐covered Chamber slides. Sperm washed through Percoll was co‐incubated with the oviductal epithelium cell cultures in a bicarbonate Tyrode's medium. During co‐incubation, sperm membrane changes, the state of tyrosine phosphorylation and motility were determined after 3, 30, 90, 180, 240 and 360 min. Significant increases in the percentage of capacitated and dead cells were observed in unbound sperm, while bound sperm remained uncapacitated, live and motile. An increasing tyrosine phosphorylation of tail proteins in bound, unbound and control sperm suspensions and a subsequent phosphorylation of head proteins in unbound and control sperm suspensions were observed. A significant difference regarding head phosphorylation (p < 0.05) was found between sperm bound to oviductal epithelium and unbound sperm. Binding occurred mainly in sperm with non‐ phosphorylated heads, while higher proportions of phosphorylated cells were found in unbound populations. The head phosphorylation progressed significantly during incubation in unbound spermatozoa (p < 0.05); however, it was suppressed in population of sperm attached to oviductal epithelium. Significant correlations between motility parameters related to hyperactivation and tail phosphorylation were found in unbound sperm. These observations support the hypothesis that spermatozoa with non‐phosphorylated heads preferentially attach to epithelial cells. It can be concluded that tyrosine phosphorylation of head membrane proteins and capacitation are delayed in canine spermatozoa being in closed contact with oviductal epithelium.  相似文献   

8 MEASUREMENT OF ARTICULAR CARTILAGE STIFFNESS IN THE FEMOROPATELLAR, TARSOCRURAL AND METATARSOPHALANGEAL JOINTS OF HORSES AS A PREDICTOR OF CARTILAGE PATHOLOGY.     

E  Garcia-Seco  DA Wilson  K Kuroki  J Cook  J Kreeger  KG Keegan 《Veterinary surgery : VS》2003,32(6):600-600

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Diagnosis of mastitis for therapy decisions.   总被引：2，自引：0，他引：2  

Philip M Sears  Kate K McCarthy 《Veterinary Clinics of North America: Food Animal Practice》2003,19(1):93-108, vi

Identifying specific groups of mastitis pathogens by their growth on selective agars can help identify the pathogens that are present in mastitic milk samples. This article addresses issues that are essential in making good use of diagnostic procedures to improve udder health on dairies.  相似文献   

Welche Faktoren regeln die Individuenzahl einer Insektenart in der Natur?     

K. Friederichs 《Journal of pest science》1929,5(10):119-123

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