Differential expression of genes encoding neurotrophic factors and their receptors along the septal‐temporal axis of the rat hippocampus     

Atsushi Toyoda  Wataru Iio  Tatsuhiko Goto  Hiroaki Koike  Takamitsu Tsukahara 《Animal Science Journal》2014,85(12):986-993

The hippocampus plays a key role in learning and emotional regulation. The hippocampus’ function varies along its septotemporal axis, with the septal pole being more frequently involved in spatial learning and memory, and the temporal pole playing a greater role in emotional behaviors. In this study, we present findings aimed at checking the expression level of the genes encoding neurotrophins and their receptors, including nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), neurotrophin‐3 (NT‐3), and their receptors (TrkA, TrkB and TrkC) in the hippocampus along the septotemporal axis. Using real‐time PCR, several different expression patterns were observed. Remarkably, the expression of both NT‐3 and TrkA genes in the septal hippocampus was higher than in the middle and temporal hippocampus. Higher expression of NT‐3 and TrkA may implicate active neurogenesis in the dentate gyrus (DG) of the septal hippocampus because more neurogenesis occurs in the septal than the temporal DG of rats. Finally, the results obtained in this study emphasize the importance of choosing the hippocampal portion along its septotemporal axis for any hippocampal molecular and biochemical experimental studies.  相似文献   

RNA polymerase II is required for RNAi-dependent heterochromatin assembly   总被引：1，自引：0，他引：1  

Kato H  Goto DB  Martienssen RA  Urano T  Furukawa K  Murakami Y 《Science (New York, N.Y.)》2005,309(5733):467-469

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Photic and circadian regulation of self-feeding activity in ayu     

Masafumi Amano  Masayuki Iigo  Kiyoshi Furukawa  Mitsuo Tabata  Kunio Yamamori 《Fisheries Science》2006,72(2):250-255

ABSTRACT:   The self-feeding rhythms of ayu Plecoglossus altivelis altivelis were examined. Individual ayu (mean body weight 40 g) were held in 60-L glass tanks equipped with self-feeders. Six of 14 fish learned self-feeding during the experiment. Under two different light–dark (LD) conditions (16 h:8 h and 8 h:16 h LD), self-feeding was synchronized to the LD cycle, and feeding occurred almost exclusively during the light phase. During exposure to constant light (LL), circadian feeding rhythms were observed. These results indicate self-feeding rhythms in ayu are restricted to the light phase under LD conditions and are controlled by the circadian clock under LL conditions.  相似文献   

Threshold change in forest understory vegetation as a result of selective fuelwood extraction in Nairobi, Kenya     

Takuya Furukawa  Kazue Fujiwara 《Forest Ecology and Management》2011,262(6):962-969

We examined whether heavy fuelwood collection can cause threshold change in understory forest community and evaluated how selective wood extraction might lead to delayed forest recovery in an urban forest of Nairobi, Kenya. Piecewise regression which represents strongest support for threshold change provided the best fit for the relationships between understory floristic composition (i.e. DCA axis 1) and human disturbance gradients (i.e. canopy cover, and distance from the slum), where threshold changes were detected at c.a. 350 m from the slum and c.a. 30% canopy cover. Only one tree species significantly indicated communities beyond the threshold while an aggressive invasive alien plant (IAP) Lantana camara was strongly represented. Total species diversity along the two human disturbance gradients peaked before the threshold was reached, suggesting that decline in species diversity along the prevailing disturbance gradient might be able to forecast threshold change. Tree species richness in the understory rapidly declined as the threshold was surpassed while other growth forms (i.e. shrubs, herbs and climbers) were relatively unaffected. The effect of selective tree cutting was indirectly impacting the forest understory as species richness pattern of preferred and non-preferred species paralleled that of trees and shrubs, respectively. Thickets of L. camara can negatively affect indigenous flora and its establishment was favored under selective fuelwood extraction removing certain tree species while leaving the IAP untouched. Shading can readily eliminate the IAP, but weak tree regeneration beyond the threshold suggested forest recovery might be delayed for longer than expected because of the interaction between selective fuelwood use and the IAP.  相似文献   

Seed yield and its components of indeterminate and determinate lines in recombinant inbred lines of soybean     

Shin Kato  Kenichiro Fujii  Setsuzo Yumoto  Masao Ishimoto  Tatsuhiko Shiraiwa  Takashi Sayama  Akio Kikuchi  Takeshi Nishio 《Breeding Science》2015,65(2):154-160

The present study was conducted to evaluate the benefits of indeterminate growth habit in breeding to improve yield potential of Japanese soybean varieties, which exclusively have determinate growth habit. Two populations of recombinant inbred lines (RILs) derived from crosses between determinate Japanese cultivars and indeterminate US cultivars were grown in Akita and Kyoto, and seed weight per plant (SW) and its components were compared between indeterminate and determinate RILs. The difference of SW between the two growth habits in RILs varied depending on maturation time. The SW of early indeterminate lines was significantly higher than that of early determinate ones in Akita, but not in Kyoto. Among yield components, the number of seeds per pod was constantly larger in indeterminate lines than that in determinate ones irrespective of maturation time. The number of seeds per plant and the number of pods per plant of the indeterminate lines were greater than those of the determinate lines in early maturation in Akita. These results suggest that the indeterminate growth habit is an advantageous characteristic in breeding for high yield of early maturing soybean varieties in the Tohoku region.  相似文献   

Rapid screening of wood chemical component variations using transmittance near-infrared spectroscopy     

Yeh TF  Yamada T  Capanema E  Chang HM  Chiang V  Kadla JF 《Journal of agricultural and food chemistry》2005,53(9):3328-3332

A rapid transmittance near-infrared (NIR) spectroscopy method was developed to predict the variation in chemical composition of solid wood. The effect of sample preparation, sample quantity (single versus stacked multiple wood wafers), and NIR acquisition time on the quantification of alpha-cellulose and lignin content was investigated. Strong correlations were obtained between laboratory wet chemistry values and the NIR-predicted values. In addition to the experimental protocol and method development, improvements in calibration error associated with utilizing stacked multiple wood wafers as opposed to single wood wafers are also discussed.  相似文献   

Can stumps tell what people want: Pattern and preference of informal wood extraction in an urban forest of Nairobi, Kenya     

Takuya Furukawa  Kazue Fujiwara  Samuel K. Kiboi  Patrick B. Chalo Mutiso 《Biological conservation》2011,144(12):3047-3054

Detailed patterns and preferences of informal wood extraction were investigated in an urban forest used by slum inhabitants of Nairobi, Kenya, as such information determines the sustainability of resource use. Instead of asking the people what they had secretly cut, a survey was conducted of the stumps and trees left in the forest to (i) evaluate how accessibility and patrolling affected spatial patterns of resource use, and to (ii) estimate a broad spectrum of species and species-specific size preferences. Both probability and size of cutting increased with better accessibility (represented by distance from the slum), and stump size exceeded that of living stem size around 1.7 km from the slum, which suggested magnified impact of tree cutting near the slum. Patrolling (represented by distance from ranger stations) had little effect in reducing the probability of cutting but was effective in reducing cut size. With the use of random effect models, a broad species preference spectrum (including less abundant and non-preferred species) was estimated, which indicated trends in vegetation change. Smaller stems were preferred for most species presumably used for domestic fuelwood, but major exploitation of large Brachylaena huillensis stems traded in the woodcarving industry was also revealed. Such detailed information on informal wood use can help forest managers to understand threats to the forests as well as the needs of local communities. This is a first step to redefining ‘acceptable’ resource use by the local community for their increased role and responsibility in sustainable forest management, especially when conventional controls of informal activities (i.e. exclusion and penalty) are not functioning well.  相似文献   

Establishment of the attenuated strain of porcine parvovirus for the live vaccine and its biological-immunological characteristics   总被引：3，自引：0，他引：3  

A Izumida  M Furukawa  M Kubota  H Takuma  K Tawara  K Kodama  Y Koda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(2):293-303

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Histopathological evaluation of the ocular-irritation potential of shampoos,make-up removers and cleansing foams in the bovine corneal opacity and permeability assay     

Masatoshi Furukawa  Takashi Sakakibara  Kouta Itoh  Kohtaro Kawamura  Satoshi Sasaki  Masao Matsuura 《Journal of toxicologic pathology》2015,28(4):243-248

The bovine corneal opacity and permeability (BCOP) assay is an alternative method to the in vivo Draize eye test in rabbits for evaluating eye irritation in vitro. Here, we compared the numerical results of the BCOP assay with the corresponding histopathology for three different corneas for each test substance, including commercially available shampoos, make-up removers and cleansing foams that contained surfactants and other ingredients. The histopathological score was defined based on the severity of lesions in the corneal epithelium. The histopathological findings and scores of the three sections for each test substance were comparable. The in vitro irritancy score (IVIS) generally corresponds to the corneal irritant potential of the test substances assigned on the basis of the histopathological findings in this study. In the present study, we characterized the histopathology of the corneal epithelium and stroma and especially showed that the corneal epithelial injury caused by test substances might be important in assessment of test substances that are mild eye irritants (category 2B) as classified by the United Nations (UN) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), as corneal lesions suggestive of classification into category 2B were localized on the border between the corneal epithelium and stroma, which contained cell elements related to assessment of prognosis of an in vivo eye injury. Histopathological assessment might be useful in predicting in vivo ocular irritation, particularly for test substances with an IVIS >3.1 but ≤25 that are classified as mild irritants (category 2B) according to the UN GHS.  相似文献   

Studies on microörganisms and their activities in soil Part. 2. Virgin and cultivated soil of the same origin     

Shuichi Ishizawa  Tatsuhiko Suzuki  Tomonori Koda  Osamu Sato 《Soil Science and Plant Nutrition》2013,59(1):129-138

The nature of soil is modified differently depending upon the artificial condition such as its utilization or management. It is therefore expected that the microbiological characteristic of soil is changed also. Greaves¹⁾ and Williams²⁾ reported that the reclamation of virgin soil brought about a change of bacterial count. Suzuki et al³⁾ observed that the kind of fungi differed between a virgin and a cultivated soil and that the vegetative mycelium was numerous in the former than in the latter. On the other hand, according to W aksman and Starkey⁴⁾, the bacterial count differed depending upon the fertility of soil. Singh⁵⁾ reported also that the number of fungi and actinomycetes was higher in a fertile than in an infertile soil. Lochhead⁶⁾, and Lochhead and Chasen studied the bacterial flora of a fertile (long-continued application of manure) and an infertile (no fertilizer for many years) soil and found that a certain difference could be observed when morphological, physiological and nutritional classification are tried.  相似文献