Ozone treatments activate defence responses against Meloidogyne incognita and Tomato spotted wilt virus in tomato     

Maria I Prigigallo  Maria Teresa Melillo  Giovanni Bubici  Petre I Dobrev  Radomira Vankova  Fabrizio Cillo  Pasqua Veronico 《Pest management science》2019,75(8):2251-2263

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Use of extensible internal device in the femur of young dogs.     

L T Justolin  S C Rahal  P P R Baptista  E S Yoname  M J Mamprim  J C C Balieiro 《Veterinary and comparative orthopaedics and traumatology》2008,21(2):133-139

An extensible internal device (EID) was developed to preserve growth plate during the treatment of fracture complications or segmental bone loss from tumour resection in children. Since this type of extensible, transphyseal, internal fixation device has only been used in a few paediatric cases; the aim of this study was to evaluate an in vivo canine study, a surgical application of this device, and its interference with longitudinal growth of the non-fractured distal femur. Ten clinically healthy two- to three-month-old poodles weighing 1.5-2.3 kg were used. Following a medial approach to the right distal femur, one extremity of the EID, similar to a T-plate, was fixed in the femoral condyle with two cortical screws placed below the growth plate. The other extremity, consisting of an adaptable brim with two screw holes and a plate guide, was fixed in the third distal of the femoral diaphysis with two cortical screws. The EID was removed 180 days after application. All of the dogs demonstrated full weight-bearing after surgery. The values of thigh and stifle circumferences, and stifle joint motion range did not show any difference between operated and control hindlimbs. The plate slid in the device according to longitudinal bone growth, in all but one dog. In this dog, a 10.5% shortening of the femoral shaft was observed due to a lack of EID sliding. The other dogs had the same longitudinal lengths in both femurs. The EID permits longitudinal bone growth without blocking the distal femur growth plate if appropriately placed.  相似文献   

A multicenter retrospective study on cryopreserved amniotic membrane transplantation for the treatment of complicated corneal ulcers in the dog     

Daniel Costa  Marta Leiva  Fernando Sanz  Victoria Espejo  Javier Esteban  Jorge Vergara  Clinio Díaz  Eduardo Huguet  Martí Cair  Jos Ríos  Maria Teresa Pea 《Veterinary ophthalmology》2019,22(5):695-702

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A pilot study comparing bone marrow aspirates and venous blood for emergency point‐of‐care blood parameters in healthy dogs     

Lindsey Ackert  Sren R. Boysen  Teresa Schiller 《Journal of Veterinary Emergency and Critical Care》2019,29(4):399-406

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Videofluoroscopic swallow study features of lower esophageal sphincter achalasia‐like syndrome in dogs     

Megan E. Grobman  James Schachtel  C. Prakash Gyawali  Teresa E. Lever  Carol R. Reinero 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(5):1954-1963

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Enteroinsular axis response to carbohydrates and fasting in healthy newborn foals     

Lindsey M. Rings  Jacob M. Swink  Laura K. Dunbar  Teresa A. Burns  Ramiro E. Toribio 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2752-2764

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E12 technique: Conventional epoxy resin sheet plastination     

Rafael Latorre  Kees de Jong  Mircea‐Constantin Sora  Octavio Lpez‐Albors  Carlos Baptista 《Anatomia, histologia, embryologia》2019,48(6):557-563

Epoxy plastination techniques were developed to obtain thin transparent body slices with high anatomical detail. This is facilitated because the plastinated tissue is transparent and the topography of the anatomical structures well preserved. For this reason, thin epoxy slices are currently used for research purposes in both macroscopic and microscopic studies. The protocol for the conventional epoxy technique (E12) follows the main steps of plastination—specimen preparation, dehydration, impregnation and curing/casting. Preparation begins with selection of the specimen, followed by freezing and slicing. Either fresh or fixed (embalmed) tissue is suitable for epoxy plastination, while slice thickness is kept between 1.5 and 3 mm. Impregnation mixture is made of epoxy E12 resin plus E1 hardener (100 ppw; 28 ppw). This mixture is reactive and temperature sensitive, and for this reason, total impregnation time under vacuum at room laboratory temperature should not last for more than 20–24 hr. Casting of impregnated slices is done in either flat chambers or by the so‐called sandwich method in either fresh mixture or the one used for impregnation. Curing is completed at 40°C to allow a complete polymerization of the epoxy‐mixture. After curing, slices can be photographed, scanned or used for anatomical study under screen negatoscope, magnification glass or fluorescent microscope. Based on epoxy sheet plastination, many anatomical papers have recent observations of and/or clarification of anatomical concepts in different areas of medical expertice.  相似文献   

Activin‐A receptor expression patterns in prepubertal goat oocytes and derived embryos     

Roser Morat  Sondes Hammami  Maria Teresa Paramio  Dolors Izquierdo 《Reproduction in domestic animals》2019,54(5):804-807

This study examines the presence of activin IIA and IIB receptors (ActR‐IIA and ActR‐IIB) by Western blotting and immunocytochemistry in immature and IVM‐oocytes, 2 to 8‐cells embryos and blastocysts from prepubertal goats. Western blotting revealed that activin receptors are synthesized during oocyte maturation and embryo development. In the immunocytochemistry experiments, no immunostaining for either receptor was detected in oocytes while both receptors were immunolabelled in all the cells of cleaved embryos. In blastocysts, while ActR‐IIA expression appeared evenly distributed in the two cell lineages, inner cell mass and trophectoderm, the ActR‐IIB immunosignal was restricted mainly to the inner cell mass. Our findings reveal the presence of activin type II receptors (ActR‐IIA and ActR‐IIB) in in vitro matured prepubertal goat oocytes and blastocyst‐stage embryos. The expression of these receptors could be a key factor in understanding differences between competent and incompetent oocytes.  相似文献   

ASSESSMENT OF INTRAOCULAR PRESSURE BY APPLANATION AND REBOUND TONOMETRY IN GUINEA PIGS OF DIFFERENT AGES     

Martí Cairó  María Teresa Peña  José Rios  Adrià Melero  Jaume Martorell  Marta Leiva 《Journal of Exotic Pet Medicine》2018,27(1):25-31

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Distribution of alveolar‐interstitial syndrome in dogs and cats with respiratory distress as assessed by lung ultrasound versus thoracic radiographs          下载免费PDF全文

Jessica L. Ward DVM  DACVIM  Gregory R. Lisciandro DVM  DACVECC  Teresa C. DeFrancesco DVM  DACVIM  DACVECC 《Journal of Veterinary Emergency and Critical Care》2018,28(5):415-428

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