Polymerase chain reaction with a primer pair in the 16S-23S rRNA spacer region for detection of Mycoplasma pulmonis in clinical isolates   总被引：2，自引：0，他引：2  

Takahashi-Omoe H  Omoe K  Matsushita S  Kobayashi H  Yamamoto K 《Comparative immunology, microbiology and infectious diseases》2004,27(2):117-128

To develop a diagnostic tool to identify Mycoplasma pulmonis (M. pulmonis) in clinical isolates, we developed a polymerase chain reaction (PCR) assay using primers specific for the 16S-23S rRNA intergenic spacer region (SR) of M. pulmonis. One pair of PCR primers reacted specifically with two reference strains of M. pulmonis tested and seven samples isolated from naturally infected rats. The primer pair did not produce PCR products of the correct size from any other rodent or human mycoplasmas or cellular DNA from rodent lungs. Specificity of the PCR assay was confirmed by Southern blotting with probe specific for the SR of M. pulmonis. The PCR assay for detection of M. pulmonis established in this study is suitable for diagnosis of M. pulmonis infection in clinical cases.  相似文献   

Cloning of bovine MAIL and its mRNA expression in white blood cells of Holstein cows     

Yamaji D  Kitamura H  Kimura K  Matsushita Y  Okada H  Shiina T  Morimatsu M  Saito M 《Veterinary immunology and immunopathology》2004,98(3-4):175-184

Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL) is known as an IkappaB protein induced after administration of bacterial lipopolysaccharide (LPS) to mice. In the present study, we cloned bovine MAIL cDNA and examined its mRNA expression in white blood cells isolated from Holstein cows. Bovine MAIL had more than 80% amino acid identities with murine and human MAILs, highly conserved ankyrin-repeat motifs and PEST-like sequences. Bovine MAIL mRNA was undetectable in isolated peripheral white blood cells, but rapidly induced (<1h) after stimulation by LPS and lipid A in vitro in a dose-dependent manner. The lipid A-induced MAIL mRNA expression was found in polymorphonuclear cells, monocytes/macrophages and total lymphocytes, but not in T-lymphocytes. MAIL mRNA was also induced in vivo in peripheral blood leukocytes of cows after intramammary injection of Escherichia coli derived from coliform mastitis. Thus, bovine MAIL, as rodent MAILs, is induced by inflammatory stimuli in specific immune cells in vitro and in vivo, suggesting a role in inflammatory responses to bacterial infection in cattle.  相似文献   

The oral administration of thermophile-fermented compost extract and its influence on stillbirths and growth rate of pre-weaning piglets     

Miyamoto H  Kodama H  Udagawa M  Mori K  Matsumoto J  Oosaki H  Oosaki T  Ishizeki M  Ishizeki D  Tanaka R  Matsushita T  Kurihara Y  Miyamoto H 《Research in veterinary science》2012,93(1):137-142

Food produced via fermentation with mesophilic bacteria has been used to confer health benefits. In contrast, mammalian physiological responses to the intake of thermophile-fermented products have not been thoroughly investigated. We examined the effects of administering a compost extract consisting of fermented marine animals with thermophiles, including Bacillaceae, to pregnant sows and piglets. Retrospective studies were performed on two different swine farms (n=330-1050 sows). The rate of stillbirth was markedly lower in all parities of the compost extract-fed group compared to those of the control group (p≦0.001). Additionally, the birth to weaning period of newborns was significantly shorter (p<0.0001), while the ratio of weanlings per liveborn piglets was increased by more than 6.5% in the compost extract-fed group. Thus thermophiles and their products in the compost extract might promote growth and reduce stillbirths of piglets during the birth to weaning period.  相似文献   

Parathyroid hormone (1-34) improves bone mineral density and glucose metabolism in Spontaneously Diabetic Torii-Lepr(fa) rats     

Kimura S  Sasase T  Ohta T  Sato E  Matsushita M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):103-105

The Spontaneously Diabetic Torii-Lepr(fa) (SDT-fa/fa) rat, a model of obese type 2 diabetes, shows obesity, hyperglycaemia and low bone mineral density (BMD). The objective of this study is to evaluate the effects of parathyroid hormone (1-34) [PTH(1-34)] on BMD and glucose metabolism in the SDT-fa/fa rat. SDT-fa/fa rats showed obesity with hyperglycaemia and decreased serum osteocalcin levels and the tibial BMD. A 4-week treatment of PTH(1-34) (20 μg/kg/day) increased the serum osteocalcin levels and the tibial BMD, and decreased the serum glucose levels without changing the serum insulin levels. These findings indicate that PTH(1-34) improved not only BMD but also glucose metabolism in SDT-fa/fa rats. This study suggests that PTH(1-34) is a novel agent for the treatment of diabetic osteoporosis.  相似文献   

Artificial neural network modeling for predicting final moisture content of individual Sugi (Cryptomeria japonica) samples during air-drying     

Ken Watanabe  Yasuhiro Matsushita  Isao Kobayashi  Naohiro Kuroda 《Journal of Wood Science》2013,59(2):112-118

Sugi (Cryptomeria japonica D. Don) lumber is known to have a large variability in final moisture content (MC_f) and is difficult to dry. This study assessed the capability of artificial neural networks (ANNs) to predict the MC_f of individual wood samples. An ANN model was developed based on initial moisture content, basic density, annual ring orientation, annual ring width, heartwood ratio and lightness (L ^* in the CIE L ^* a ^* b ^* system). The performance of the ANN model was compared with a principal component regression (PCR) model. The ANN model showed good agreement with the experimentally measured MC_f with a higher correlation coefficient (r) and a lower root mean square error (RMSE) than the PCR model, demonstrating the importance of nonlinearity of the variables and the higher capability of the ANN model than the PCR model. By adding redness (a ^* ) and yellowness (b ^* ) and drying time to the input variables of ANNs, r and RMSE values were improved to 0.98 and 1.2 % for the training data set, and 0.85 and 2.2 % for the testing data set, respectively. Although the developed ANNs are available under the limited conditions of this study, our results suggest that the ANNs proposed offer reliable models and powerful prediction capability for the MC_f, even though wood properties vary considerably and their complex interrelations are not fully elucidated.  相似文献   

Mean retention time of digesta in the different segments of the equine hindgut     

Makoto MIYAJI  Koichiro UEDA  Hiroki NAKATSUJI  Teruo TOMIOKA  Yasuo KOBAYASHI  Hiroshi HATA  Seiji KONDO 《Animal Science Journal》2008,79(1):89-96

Six Thoroughbred horses were used to determine the mean retention time (MRT) of digesta in the different segments of the hindgut. The horses were fed timothy hay or silage in equal amounts (1.6% bodyweight [BW]/day) every 3 h/day. Hay or silage labeled with the rare earth elements cerium, dysprosium, neodymium, ytterbium, lanthanum, samarium and praseodymium, were fed to the horses 36, 30, 24, 18, 12, 6 and 3 h before slaughter, respectively. Just after slaughter, digesta samples in the different segments were collected and determined for the contents of the rare earth elements. Using these data, a method to calculate the MRT in the different segments was proposed. There were no significant differences between diets in the MRT of each segment. The averaged MRT of hay and silage in the cecum, right ventral colon, left ventral colon, left dorsal colon, right dorsal colon and small colon was 2.9, 3.1, 5.9, 1.0, 4.0, and 4.0 h, respectively. The dry matter weight of the digesta was related to the MRT in the right ventral colon (r = 0.94, P = 0.005), left ventral colon (r = 0.87, P = 0.03), left dorsal colon (r = 0.98, P < 0.001) and right dorsal colon (r = 0.95, P = 0.004), but it was not related to the MRT in the cecum and small colon.  相似文献   

Evaluation of three hormonal protocols for anovulatory lactating cows under regulations restricting the use of estrogenic compounds          下载免费PDF全文

Guang‐Min Yu  Yue Wu  Xian‐Long Wang  Shuan Zhao  Teruo Maeda  Shen‐Ming Zeng 《Animal Science Journal》2018,89(4):640-647

When European Union regulations restricted the use of estrogenic compounds in food‐producing animals, refined hormonal protocols were no longer applicable for anovulatory cows. However, Ovsynch and its adaptations are routinely and uniformly applied to all cows regardless of ovarian function. To evaluate their efficacy on anovulatory cows, 143, 147 and 144 anovulatory cows received Ovsynch, Presynch and G6G protocols, respectively. In comparison, 150 cyclic cows were bred without using a synchronized protocol. Results showed that cows in the Presynch group had luteolysis responding to the last prostaglandin F_2α (PGF_2α) injection greater than the Ovsynch group. The serous progesterone levels at the first gonadotropin‐releasing hormone of Ovsych and the last PGF_2α injection was greater in the G6G group than the other two hormonal treatment groups. Concentrations of Ca²⁺ and total protein in cervical mucus in all three hormone‐treated groups before artificial insemination (AI) were significantly different from the controls. The G6G group obtained a greater pregnancy rate compared with Ovsynch and Presynch, but significantly less than the controls. For open cows in the Ovsynch group, estrus rate within 24 days after the first AI was significantly less than the controls. In conclusion, the G6G treatment resulted to better reproductive performance in anovulatory cows.  相似文献   

有机肥和化肥对菠菜产量及品质的影响   总被引：1，自引：0，他引：1  

帕提曼·阿不都热合曼  松中照夫  秦勇 《北方园艺》2007,(11):28-30

研究了施用牛粪堆肥和化肥对菠菜产量和品质方面的影响.结果表明:施用牛粪堆肥可以提高菠菜的维生素C、全糖和还原糖的含量,降低菠菜硝态氮的含量,但增加产量的作用不明显.施用化肥虽然可以提高菠菜的产量,但菠菜的维生素C、全糖和还原糖的含量降低,硝态氮的含量上升.  相似文献   

Extraction of silver from soils by chloride solutions     

Satoshi Saeki  Masatsugu Kubota  Teruo Asami 《植物养料与土壤学杂志》1998,161(2):105-107

Using chloride solutions as extractants, 6.4–18.4% of Ag were extracted from polluted Annaka and Fuchu soils, whereas only 0.06–0.08% Ag were extractable using NH₄OAc, NH₄NO₃ or (NH₄₎₂SO₄ solution. In the case of unpolluted Konosu and Nagano soils, 28.3–47.0% of Ag were extracted by KCl and 0.7–1.0% of Ag were by NH₄OAc. The silver extracted with chloride solution was assumed to be derived from residual fractions.  相似文献   

Swimming depths of the giant jellyfish <Emphasis Type="Italic">Nemopilema nomurai</Emphasis> investigated using pop-up archival transmitting tags and ultrasonic pingers     

Naoto Honda  Toshihiro Watanabe  Yoshiki Matsushita 《Fisheries Science》2009,75(4):947-956

The swimming depths of 12 individual Nemopilema nomurai with bell diameters of 0.8–1.6 m were investigated using pop-up archival transmitting tags and ultrasonic pingers, and the validity of the research method was evaluated. The N. nomurai studied frequently showed vertical movement, with the swimming depth ranging from 0 to 176 m, The mean swimming depths of most individuals were less than 40 m. The swimming depths of N. nomurai in the northern Japan Sea in the winter were mostly deeper than those of this species in the southern Japan Sea in the autumn. This result suggests that the range of the depths almost depends on the vertical structure of the ocean. Swimming depths during the nighttime were significantly deeper than those during the daytime. More specifically, during the daytime, the swimming depths in the afternoon tended to be shallower than those in the morning, while during the nighttime, the swimming depths after midnight were deeper than those before midnight.  相似文献