Molecular Monitoring of hrpB-disrupted Mutant of Ralstonia solanacearum in Tomato Plants     

Yoshinori MATSUDA  Hideyoshi TOYODA  Yasunari KATO  Koji KAKUTANI  Takayuki NAKANISHI  Miki BINGO  Teruo NONOMURA  Seiji OUCHI 《Journal of General Plant Pathology》2000,66(1):59-63

A nonpathogenic mutant of Ralstonia solanacearum was produced by the insertion of transposon Tn4431. The mutagenized gene was then cloned from a genomic DNA library by the gene tagging method, using the labeled lux operon located on Tn4431 of pUCD623 as a hybridization probe. From nucleotide sequence analysis of the transposon-inserted genomic clone, the hrpB gene was shown to be disrupted by the inserted transposon. Tomato plants were inoculated with the hrpB-disrupted mutant bacteria, for which multiplication and translocation were then monitored using the colony hybridization method. In addition, the original pathogenic bacteria in which the lux operon had been functionally ligated with the genomic promoter were also used for inoculation and traced by their bioluminescence. Multiplication of the hrpB-disrupted mutant was suppressed initially in the invaded root tissues and then in upper hypocotyl after translocation, suggesting that the pathogenic strain of R. solanacearum overcomes at least two steps of host responses expressed in root and hypocotyl tissues. Thus, our approach for molecular monitoring of the bacteria enabled us to precisely analyze the infection behavior of the pathogenic bacteria in planta. Received 16 April 1999/ Accepted in revised form 10 August 1999  相似文献   

A 13-week subchronic toxicity study of 2-(l-menthoxy)ethanol in F344 rats     

Takeshi Toyoda  Kohei Matsushita  Hirotoshi Akane  Tomomi Morikawa  Kumiko Ogawa 《Journal of toxicologic pathology》2021,34(4):309

2-(l-Menthoxy)ethanol has been frequently employed as a flavoring agent; however, data regarding 2-(l-menthoxy)ethanol toxicity remain limited. We performed a 13-week subchronic toxicity study of 2-(l-menthoxy)ethanol in male and female F344 rats, with doses of 0, 15, 60, or 250 mg/kg body weight (BW)/day orally administered by gavage using corn oil as the vehicle. No significant toxicological changes in general condition, body weight, or food intake were observed in any groups. The hematological assessment showed decreased hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin and increased platelet count in the male 250 mg/kg group. Serum biochemistry revealed elevated total cholesterol in the 250 mg/kg group of male and female rats, reduced triglyceride in the female 250 mg/kg group, and increased total protein in the male 250 mg/kg group, indicating effects on lipid metabolism and protein synthesis. For organ weights, absolute and relative weights of the liver and adrenal glands were increased in the 250 mg/kg group of both sexes and the male 250 mg/kg group, respectively. Histopathological analysis showed chronic nephropathy in the male 15 mg/kg or higher groups, with increased absolute and relative kidney weights, as well as elevated serum creatinine, in the male 60 and 250 mg/kg groups. However, eosinophilic granules containing α_2u-globulin were identified in proximal tubules, suggesting α_2u-globulin nephropathy specific to male rats and without toxicological significance. These results indicated that the no-observed-adverse-effect level of 2-(l-menthoxy)ethanol was 60 mg/kg BW/day for both sexes.  相似文献   

Migration of Bursaphelenchus xylophilus in cortical and xylem axial resin canals of resistant pines          下载免费PDF全文

J. A. Son  N. Matsushita  T. Hogetsu 《Forest Pathology》2015,45(3):246-253

Migration of the pine wood nematode (PWN), Bursaphelenchus xylophilus, in susceptible and resistant pines was investigated at the tissue level. PWN was inoculated onto the top cross‐cut surface of 20‐cm stem cuttings of susceptible Pinus thunbergii and resistant pines (P. strobus, P. rigida and P. thunbergii of a resistant family Namikata‐(t)‐73 (half‐sib)). PWNs were mainly distributed in cortical resin canals of susceptible P. thunbergii down to 15 cm from the inoculated surface by 6 h after inoculation (HAI) and all tissues (including cortical and xylem resin canals) down to the bottom at 192 HAI. In P. strobus, P. rigida and P. thunbergii family Namikata‐(t)‐73 (half‐sib), PWN was distributed in cortical resin canals down to 5 cm by 6 HAI and down to the base at 192 HAI. However, the distribution of PWN in xylem resin canals of the resistant pines was restricted near inoculated surfaces down to 5 cm, even at 192 HAI. These results demonstrated that migration of PWN in resistant pines was slowed in cortical resin canals and restricted in xylem axial resin canals, features which may be associated with the resistance.  相似文献   

Spontaneous Nephroblastoma with Lung Metastasis in a Rat     

Yuko Ito  Kohei Matsushita  Takuma Tsuchiya  Yukari Kohara  Tsuyoshi Yoshikawa  Makoto Sato  Keisuke Kitaura  Satoshi Matsumoto 《Journal of toxicologic pathology》2014,27(1):91-95

This report describes a spontaneous nephroblastoma with lung metastasis in a 10-week-old male Crl:CD(SD) rat. Macroscopically, a white mass in the kidney and two white masses in the lung were observed. Histopathologically, the renal mass was located in the cortex of a kidney, and it caused pressure on the surrounding renal parenchyma. Three components could be distinguished in the tumor: blastemal, epithelial (primitive glomerular/tubular structures) and mesenchymal (neoplastic connective tissues) elements. Immunohistochemically, the tumor cells were positive for Wilms tumor 1 protein (WT1) and vimentin. Metastasis was found in the lung. Thus, the case was diagnosed as a nephroblastoma with lung metastasis.  相似文献   

Suramin protection of T cells in vitro against infectivity and cytopathic effect of HTLV-III   总被引：24，自引：0，他引：24  

H Mitsuya  M Popovic  R Yarchoan  S Matsushita  R C Gallo  S Broder 《Science (New York, N.Y.)》1984,226(4671):172-174

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Determination of 1-deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection   总被引：9，自引：0，他引：9  

Kimura T  Nakagawa K  Saito Y  Yamagishi K  Suzuki M  Yamaki K  Shinmoto H  Miyazawa T 《Journal of agricultural and food chemistry》2004,52(6):1415-1418

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.  相似文献   

Use of genetic markers to identify the illegal trade of billfish in the second largest fishing warehouse of Latin America     

Carlos Egberto Rodrigues Júnior  Sandro Natal Daniel  Diego Galetti Martins  Diogo Teruo Hashimoto  Fausto Foresti  Fabio Porto‐Foresti 《水产资源保护:海洋与淡水生态系统》2020,30(6):1251-1254

1. Billfish are oceanic pelagic species that are often caught by tuna fleets and are of great interest for sport fishing. Two species of billfish have specific legislation prohibiting their marketing and export in Brazil.
2. DNA barcoding is a universal system of molecular identification based on a sequence of mitochondrial DNA cytochrome oxidase subunit I (COI), which serves as a diagnostic genomic marker in each species.
3. The barcode DNA technique was used to identify billfish marketed in the second largest fishing warehouse in Latin America, the CEAGESP (Companhia de Entrepostos e Armazéns Gerais de São Paulo), located in São Paulo, Brazil. Seventy‐nine samples of billfish were collected during three inspection visits carried out by Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis.
4. After DNA sequencing, 70 samples (88.60%) were identified to the species level; 21 (30.00%) were identified as Xiphias gladius, 43 (61.42%) as Istiophorus platypterus and six (8.57%) as Kajikia albida. The sale of this latter species is prohibited in Brazil and it is considered Vulnerable on the list of endangered species of the IUCN and in the official list of species of endangered Fauna – Fish and Aquatic Invertebrates.
5. Molecular analyses proved to be very efficient at uncovering irregularities in the identification of the white marlin (K. albida), which was traded illegally in CEAGESP, demonstrating the ineffectiveness of the current monitoring techniques used and emphasizing the need for the adoption of better public policies for the conservation of the species.

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Effects of solar ultraviolet radiation on biomass production and pigment contents of <Emphasis Type="Italic">Spirulina platensis</Emphasis> in commercial operations under sunny and cloudy weather conditions     

Hongyan?Wu  Kunshan?GaoEmail author  Zengling?Ma  Teruo?Watanabe 《Fisheries Science》2005,71(2):454-456

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Clonal variation in heartwood norlignans of <Emphasis Type="Italic">Cryptomeria japonica</Emphasis>: evidence for independent control of agatharesinol and sequirin C biosynthesis     

Nobumasa Bito  Ryogo Nakada  Eitaro Fukatsu  Yasuyuki Matsushita  Kazuhiko Fukushima  Takanori Imai 《Annals of Forest Science》2011,68(6):1049-1056

• Introduction   

In Cryptomeria japonica, heartwood properties are considered to be affected by specific extractives. It remains unclear whether traits of specific heartwood compounds are under genetic control.  相似文献   

Characterization of <Emphasis Type="Italic">Inago1</Emphasis> and <Emphasis Type="Italic">Inago2</Emphasis> retrotransposons in <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis>     

Edmundo SanchezJr.  Kozo Asano  Teruo Sone 《Journal of General Plant Pathology》2011,77(4):239-242

From the genome of a Japanese field isolate of the rice blast fungus, Magnaporthe oryzae, we newly identified Inago1 and Inago2 LTR retrotransposons. Both elements were found to be Ty3/gypsy-like elements whose copies were dispersed within the genome of Magnaporthe spp. isolates infecting rice and other monocot plants. Southern hybridization patterns of nine re-isolates derived from conidia of the strain Ina168 produced after a methyl viologen treatment were not changed, indicating that the insertion pattern of Inago elements is relatively stable.  相似文献