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11.
Three ichthyotoxic peptides, pardaxins P-I to P-3, have been isolated from the defense secretion of the sole Pardachirus pavoninus. Pavoninins, the steroid glycosides with shark-repelling ability, had previously been isolated therefrom. Each pardaxin consists of 33 amino acid residues having a distinctly hydrophilic carboxyl terminal region and a predominantly hydrophobic remainder; the pardaxin is thus strongly surfactant. These peptides show marked physical and pharmacological similarities to melittin, the major active constituent of bee venom, yet they lack sequence homology. They are probably also responsible for the predator-repelling property of the sole. 相似文献
12.
M Nakanishi A C Wilson R A Nolan G C Gorman G S Bailey 《Science (New York, N.Y.)》1969,163(868):681-683
Pieces of chicken heart or skeletal muscle were placed in a dilute solution of the antimicrobial agent 2-phenoxyethanol and stored at room temperature. Under these conditions, the serum albumin, lactate dehydrogenase, and malate dehydrogenase in these tissues survived in easily detectable amounts for at least 2 weeks. The surviving proteins appeared to be identical with those of fresh tissues in physical, catalytic, and immunological properties. Phenoxyethanol also preserved heart and muscle proteins of representatives of other vertebrate classes. Tissue samples collected in the analysis by biochemical taxonomists. 相似文献
13.
Simultaneous gas chromatographic determination of dibutyltin and tributyltin compounds in biological and sediment samples 总被引:1,自引:0,他引:1
T Tsuda H Nakanishi T Morita J Takebayashi 《Journal of the Association of Official Analytical Chemists》1986,69(6):981-984
A method is described for the simultaneous determination of nanogram amounts of dibutyltin and tributyltin compounds in biological and sediment samples. These compounds are converted to the corresponding chlorides with HCl, extracted with ethyl acetate-hexane (3 + 2) for biological samples and with hexane for sediment samples, and hydrogenated with sodium borohydride. The corresponding hydrides, Bu2SnH2 and Bu3SnH, are detected by electron-capture gas chromatography after cleanup by silica gel column chromatography. Detection limits are 1.0-2.0 and 0.5-1.0 ng/g, respectively, for biological and sediment samples. 相似文献
14.
T Tsuda H Nakanishi T Morita J Takebayashi 《Journal of the Association of Official Analytical Chemists》1985,68(5):902-905
A method was developed for simultaneous gas chromatographic determination of sorbic acid, dehydroacetic acid, and benzoic acid used as preservatives, and succinic acid, fumaric acid, malic acid, and tartaric acid used as acidulants in soft drinks and jams. A sample was dissolved in NH4OH-NH4Cl pH 9 buffer solution, and an aliquot of the solution was passed through a QAE-Sephadex A 25 column. The column was washed with water, and the carboxylic acids were eluted with 0.1N HCl. Sorbic acid, dehydroacetic acid, and benzoic acid were extracted with ethyl ether-petroleum ether (1 + 1), and determined on a 5% DEGS + 1% H3PO4 column. Succinic acid, fumaric acid, malic acid, and tartaric acid in the lower layer were derivatized with N,O-bis(trimethylsilyl)acetamide and trimethylchlorosilane, and determined on a 3% SE-30 column. Recoveries from soft drink and jam samples fortified with 0.1% each of 7 carboxylic acids ranged from 92.4 to 102.6% for preservatives, and from 88.1 to 103.2% for acidulants. 相似文献
15.
Hiroshi Hara Moritsugu Kitamura Atsuko Mori Izumi Noguchi Tsuyoshi Ohizumi Sinya Seto Tadashi Takeuchi Teruyuki Deguchi 《Water, air, and soil pollution》1995,85(4):2307-2312
Precipitation chemistry in Japan was discussed on a wet-only sample database obtained in a nationwide survey from April 1989 to March 1993. Wet-only samples were collected at 29 stations over Japan on a biweekly basis. Commonly determined chemical parameters were measured in laboratories. The volume-weighted annual mean pH at each site ranged from 4.50 to 5.83 with a mean of 4.76. Concentration ranges and means (parenthesized) on an equivalent basis for major ions were as follows: nss-SO4
2–; 5.2–58.9 (38.6), NO3
–; 1.8–25.0 (14.1), NH4
+; 0.55–29.8 (18.3), nss-Ca2+; 2.0–34.5(14.2), Na+; 6.4–275.3 (49.1), Cl–; 13.7–322.4 (63.5) eq L–1. Acid-base relationships for Phase-II records were quantitatively discussed in terms of three measures: pH, fractional acidity, and our proposed pAi. 相似文献
16.
17.
Hohdatsu T Nakanishi T Saito I Koyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):129-131
We investigated the relationship between CD8+ T cell anti-feline immunodeficiency virus (FIV) activity and FIV proviral DNA load integrated in mononuclear cells. The anti-FIV activity and the proviral DNA load were correlated, and the number of proviral DNA copies was high in cats with decreased anti-FIV activity. Particularly, no anti-FIV activity was detected in the cats staged as having an acquired immunodeficiency syndrome (AIDS)-related complex or AIDS, and the number of proviral DNA copies was obviously increased compared to those in the cats in the asymptomatic stage. These results suggest that decreased anti-FIV activity destroys the control of in vivo FIV replication, which leads to an increased proviral DNA load with the progression of the clinical stage of disease. 相似文献
18.
Nakanishi M Kuwamura M Yamate J Fujita D Sasai H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(9):939-941
A 6-year-old female ferret had a firm mass 2 cm in diameter in the pyloric region of the stomach. Histopathologically, the mass was composed of neoplastic proliferation of well-differentiated epithelial cells, showing tubular or glandular growth patterns. Osseous metaplastic foci were often found in the tumor. Tumor cells showed a positive reaction for immunohistochemistry against bone morphogenetic protein-6, an osteogenic factor. A diagnosis of gastric adenocarcinoma with ossification was made. 相似文献
19.
Teruhisa Yotsuya Takao Ichii Minoru Sawano Tetsu Nakanishi Takeshi Ozaki 《Scientia Horticulturae》1984,24(2):177-184
Leaf buds of Japanese pear were collected in early June and early November and regarded as summer and winter dormant buds, respectively. Bud explants with and without scales were prepared from each of them, and cultured in vitro for 75 days at 25°C with 14 h photoperiod, on a medium either without growth regulators, or supplied with BA and GAs (GA3 and GA4+7), singly or in combination.When either BA or GA4+7 was contained in the medium, bud expansion occurred. Thereafter, summer dormant buds grew into shoots in the presence of BA, while winter dormant buds, although they swelled profusely, remained in a rosette. In the presence of BA, GA4+7 markedly stimulated shoot elongation of summer dormant buds, but GA3 did not. In winter dormant buds, GA4+7 not only failed to stimulate shoot elongation, but also interfered with the BA-induced swelling described above.The presence of bud scales delayed expansion of summer dormant buds, while it had little effect on winter dormant buds. The delaying effect of scales on expansion of summer buds was effectively removed by application of GA4+7 to the medium. 相似文献
20.
Yoshinori?KobayashiEmail author Takanori?Tsukamoto Naohiko?Miyai Yoshinari?Nakanishi 《Journal of General Plant Pathology》2004,70(5):295-296
In October 2001, anthracnose caused by Colletotrichum acutatum Simmonds ex Simmonds was found on three-leaf akebia (Akebia trifoliata) in Saitama, Japan. This is the first report of anthracnose on three-leaf akebia caused by C. acutatum. 相似文献