Expression of two novel cytochrome P450 3A131 and 3A132 in liver and small intestine of domestic cats     

Honda K  Komatsu T  Koyama F  Kubota A  Kawakami K  Asakura H  Uno Y  Kitazawa T  Hiraga T  Teraoka H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1489-1492

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Hydrus-1D模型在田间水循环规律分析中的应用及改进   总被引：9，自引：4，他引：5  

马欢  杨大文  雷慧闽  蔡建峰  楠田哲也 《农业工程学报》2011,27(3):6-12

为分析农田水分运移规律,以华北平原位山引黄灌区典型田间为研究对象,应用Hydrus-1D模型连续模拟了 2006－2009年的田间水分运移过程。对模型中表面阻抗的计算方法进行了改进,从而改善了蒸散发的模拟结果,与涡度相关系统的实测蒸散发数据对比表明了改进的合理性。对蒸散发的分析表明,对于种植季节性作物的农田,叶面积指数（leaf area index,LAI）是影响蒸散发季节变化过程的重要因素,而气象条件主要决定其在较短时间尺度（如一周）内的波动。与时域反射仪（time domain reflectometry,TDR）实测土壤含水率的对比表明,模型对土壤含水率的模拟精度较高。水量平衡分析结果表明,降雨和灌溉的80%由蒸散发消耗,在汛期农田通过排水发生田间渗漏损失。  相似文献   

Relation between stockperson behavior toward cows in a holding area and milk yields     

Haruka Saito  Tetsuya Seo 《Animal Science Journal》2020,91(1)

To assess the effects of negative and positive behaviors on milk yields, this study examined the attitudes and behaviors of stockpersons toward their cows and milk yields at a farm where 15 workers milked 67 cows as rotating shift work. At a holding area when moving cows to the parlor, stockperson behaviors were observed at 34 milkings. Behaviors were classified as negative (NEG) or positive (POS): NEG were attempts to move cows using touching (NT), vocalization (NV), and gestures (NG); POS were communications without attempting to move cows, consisting of touch (PT) or vocalization (PV). Numbers of NV and NEG were negatively correlated with milk yields of first parity (p < .05). Average milk yields of first parity and all cows were higher when PT were observed (p < .001, p < .0001) and POS were observed (p < .001 and p < .0001). Numbers of PT were positively correlated with milk yields of first parity (p < .05). Average milk yields of second parity were higher when PV was observed (p < .01). Positive behaviors, especially touching, are related to higher herd milk yields. Negative behaviors such as negative vocalizations are related to lower yields of younger cows.  相似文献   

Spontaneous hyaline glomerulopathy in a young Slc:ICR mouse     

Hironobu Nishina  Akane Kashimura  Tetsuya Sakairi  Satomi Nishikawa  Mao Mizukawa  Hiroko Sato 《Journal of toxicologic pathology》2020,33(4):303

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Two Types of Grapevine Yellow Speckle Viroid 1 Isolated from Commercial Grapevine Had the Nucleotide Sequence of Yellow Speckle Symptom-inducing Type     

Teruo SANO  Tetsuya KOBAYASHI  Akira ISHIGURO  Yoshie MOTOMURA 《Journal of General Plant Pathology》2000,66(1):68-70

Grapevine yellow speckle viroid 1 (GYSVd 1) was first detected in Japan from commercial grapevines. The viroid seems to be spreading in Japanese commercial vineyards but without causing noticeable disease symptoms. Two Japanese GYSVd 1 isolates from the cultivars Campbell and Steuben were sequenced. The Campbell isolate is included in the type 2 variant of GYSVd 1, and the Steuben isolate is included in the type 3. Both of the isolates were grouped into the yellow speckle-inducing variants in the nucleotide sequence ; however, the etiological importance of this viroid on the viticulture of Japan has not yet been determined. Received 14 June 1999/ Accepted in revised form 25 August 1999  相似文献   

Pathology of active hepatitis in athymic nude mice caused by a mutant strain of mouse hepatitis virus, MHV-2-CC     

N Goto  T Inoue  N Hirano  A Sato  K Fujiwara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(6):971-977

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Transfer of antibodies against viruses of calf diarrhea from cows to their offspring via colostrum     

T Murakami  N Hirano  A Inoue  K Chitose  K Tsuchiya  K Ono  Y Naito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(3):507-510

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Relationship between pulmonary arterial pressure and pulmonary thromboembolism associated with dead worms in canine heartworm disease.     

Y Hirano  H Kitagawa  Y Sasaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):897-904

To examine effects of thromboemboli due to dead worms on pulmonary arterial pressure (PAP), 20 to 50 dead heartworms were inserted into the pulmonary arteries of 4 heartworm uninfected dogs (uninfected group) and 11 dogs infected with heartworms (infected group). In the uninfected group, the mean PAP rose 1 week after worm insertion (10.9 to 166. mmHg), but it recovered by the 4th week. Clinical signs, hemodynamics and blood gas findings also deteriorated at the 1st week, but recovered at the 4th week. Angiographic and pathological findings indicated that blood flow recovered through the spaces between thromboemboli and vessel walls at the 4th week. The infected dogs were divided into three groups. In the infected-I group (5 dogs), the intimal lesions of the pulmonary arteries were slight, and clinical and laboratory findings showed changes similar to those of the uninfected group. In the infected-II group (4 dogs), the pulmonary arterial lesions were severe and the mean PAP was higher (25.7 mmHg) than in the uninfected group before worm insertion. An increase in PAP (34.1 mmHg) and worsening of clinical and laboratory findings were noticed till the 4th week. Thromboemboli adhered extensively to the vessel walls. Two dogs in the infected-III group died of severe dyspnea on the 9th and 10th day, and the mean PAP rose remarkably at the 1st week (from 19.4 to 28.2 mmHg). Severe pulmonary parenchymal lesions with edema or perforation were observed. From the above results, it was clarified that effects of dead worms on PAP and clinical signs depended on the severity of pulmonary arterial lesions before worm insertion.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Generation of PDX‐1 mutant porcine blastocysts by introducing CRISPR/Cas9‐system into porcine zygotes via electroporation     

Fuminori Tanihara  Maki Hirata  Nhien T. Nguyen  Quynh A. Le  Takayuki Hirano  Tatsuya Takemoto  Michiko Nakai  Dai‐ichiro Fuchimoto  Takeshige Otoi 《Animal Science Journal》2019,90(1):55-61

Recently, we established the GEEP (“gene editing by electroporation of Cas9 protein”) method, in which the CRISPR/Cas9 system, consisting of a Cas9 protein and single guide RNA (sgRNA), is introduced into pig zygotes by electroporation and thus induces highly efficient targeted gene disruption. In this study, we examined the effects of sgRNA on the blastocyst formation of porcine embryos and evaluated their genome‐editing efficiency. To produce an animal model for diabetes, we targeted PDX‐1 (pancreas duodenum homeobox 1), a gene that is crucial for pancreas development during the fetal period and whose monoallelic disruption impairs insulin secretion. First, Cas9 protein with different sgRNAs that targeted distinct sites in the PDX‐1 exon 1 was introduced into in vitro‐fertilized zygotes by the GEEP method. Of the six sgRNAs tested, three sgRNAs (sgRNA1, 2, and 3) successfully modified PDX‐1 gene. The blastocyst formation rate of zygotes edited with sgRNA3 was significantly (p < 0.05) lower than that of control zygotes without the electroporation treatment. Our study indicates that the GEEP method can be successfully used to generate PDX‐1 mutant blastocysts, but the development and the efficiency of editing the genome of zygotes may be affected by the sgRNA used for CRISPR/Cas9 system.  相似文献   

Relationship among ovarian follicular status,developmental competence of oocytes,and anti‐Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts     

Fuminori Tanihara  Maki Hirata  Satoru Iizuka  Shinya Sairiki  Masahiro Nii  Nhien Thi Nguyen  Quynh Anh Le  Takayuki Hirano  Takeshige Otoi 《Animal Science Journal》2019,90(6):712-718

The aim of this study was to investigate the ovarian follicular development, developmental competence of oocytes, and plasma anti‐Müllerian hormone (AMH) levels of Japanese wild boar crossbred (wild hybrid) gilts, whose litter size is inferior to that of European breeds. Ovary and plasma samples were collected from two different breeds of gilts (wild hybrid and Large White breeds). The ovaries from the wild hybrid gilts had a lower average numbers of secondary follicles and vesicular follicles in ovarian cross‐sections and of good quality oocytes collected from ovarian follicles as compared with those from Large White gilts (p < 0.05). The development rate to the blastocyst stage of good quality oocytes after in vitro maturation, fertilization and culture was also lower (p < 0.05) in wild hybrid gilts than in Large White gilts. Plasma AMH levels with >0.16 ng/ml were detected in 8.3% of the examined wild hybrid gilts and 33% of the Large White gilts. These results indicate that the low reproductive performance of wild hybrid breed may result in part from low numbers of vesicular follicles and good quality oocytes, and low developmental competence of oocytes. Moreover, plasma AMH levels may support low number of vesicular follicles in ovaries of wild hybrid gilts.  相似文献