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21.
Shweta Shambhavi Rakesh Kumar Rajeev Padbhushan Gayatri Verma Surya Prakash Sharma Sanjay K. Sharma Raj Paul Sharma 《Soil Use and Management》2020,36(3):507-523
This present investigation took place on a continuing long-term fertilizer experiment, initiated in 1972 at the experimental farm of the College of Agriculture CSK HPKV, Palampur, aimed at studying nutrient dynamics of micronutrients, especially Zn, after continuous use of chemical fertilizers and amendments over the previous 36 years in an acid Alfisol under a maize–wheat system. Treatments investigated were as follows: T1: Control; 100% N; 100% NP; 100% NPK (optimal application - 120:26:33(maize)/25(wheat)); 100% NPK + FYM (10 t ha−1 to the maize crop); T6: 100% NPK + lime (900 kg ha−1); T7: 100% NPK + Zn (25 kg ha−1 as ZnSO4); T8: 100% NPK + Hand weeding; T9: 100% NPK (-S); T10: 150% NPK (super-optimal application); and T11: 50% NPK (sub-optimal application). Different forms of zinc in soil were determined through a sequential extraction method. Results revealed that previous applications of high-analysis fertilizers and amendments caused a marked depletion in the pools of Zn as compared to buffer plots. All pools of Zn as well as crop productivity and Zn uptake were noticeably greater in farmyard manure (FYM)-amended plots compared with plots not receiving fertilizer. The residual fraction was the dominant form but organically bound and exchangeable forms were found to play major role in nutrient supply, crop productivity and nutrient uptake. Correlation and regression analysis studies showed that organic forms constituted the most important pool contributing towards variation in yield and uptake by maize and wheat crops. Exchangeable and organically bound forms contributed significantly towards the availability of DTPA-extractable Zn in soil. 相似文献
22.
Kusmita Lia Mutiara Erlita Verdia Nuryadi Handung Pratama Petrick Ariska Wiguna Awang Surya Radjasa Ocky Karna 《International Aquatic Research》2017,9(1):61-69
International Aquatic Research - Marine bacteria produce natural pigments; however, the ability of marine bacterial symbionts to produce natural pigments has been less studied. Marine bacteria... 相似文献
23.
Yonvitner Yonvitner Josep LIoret Mennofatria Boer Rahmat Kurnia Surya Gentha Akmal Ernik Yuliana Diarsi Eka Yani Sílvia Gmez Ludivica Endang Setijorini 《Fisheries Management and Ecology》2020,27(5):472-480
Although small‐scale fisheries (SSF) play an important socio‐economic role in developing nations, overfishing seems to be increasing the risk of stock vulnerability. This study aims to quantify the pressure of SSF on fish stocks in Sunda Strait (Indonesia) using several biological indicators that are important in quantifying fishing pressure. Data on these indicators were collected monthly for three years (2012–2014) in one of the main fishing ports of the area. The results provide evidence that, although SSF would appear to be the most environmentally sustainable of all the fishing techniques being used today in the coastal waters of Indonesia, the impact of SSF fishing on juvenile fishes in certain areas such as the Sunda Strait must not be underestimated. The results also show the need to protect immature fish of species that are not only commercialised but are also important in subsistence fisheries. Although further studies are needed to assess the impact of SSF on fish stocks in the area, it is suggested management recommendations that include the implementation of marine‐protected areas in nursery grounds and establishing minimum landing sizes well above the size‐at‐maturity for each species, are needed. 相似文献
24.
Swati Sucharita Dash Basanta Kumar Das Phalguni Pattnaik Surya Kanta Samal Swagatika Sahu Subrato Ghosh 《Journal of the World Aquaculture Society》2009,40(2):236-247
Flavobacterium columnare is an important pathogen of freshwater fishes often causing high mortality. Seven strains of F. columnare have been isolated from gill necrosis, skin lesions and internal organs of Catla catla, Labeo rohita, Cirrhinus mrigala, Carassius auratus, Anabas testudineus and Clarias batrachus and characterized by biochemical reactions and serological tests viz indirect enzyme‐linked immunosorbent assay (ELISA), Dot‐ELISA, agglutination test. All the strains showed binding to Congo red dye as well as hemolysis. All the seven strains were susceptible to amikacin, gentamycin and ofloxacin. In vivo LD50 dose of virulent strain MS2 was found to be 6 × 104 CFU/mL after experimental infection to L. rohita. Serologically all the seven strains showed a positive result to agglutination, Dot‐ELISA and indirect ELISA. The agglutination titer was found to be in the range of 256‐131,072. The lysozyme activity of hyperimmune sera was found to be 39.37 ± 0.80 units/mL. This is the first extensive study that report about the various strains of F. columnare from freshwater fishes of Eastern India and their differentiation by biochemical and serological methods. 相似文献
25.
All cycads are strictly dioecious with a long juvenile stage. Currently, there is no method available to determine the sexuality of seedlings prior to the onset of cone formation. This study aimed to develop a sex specific Random Amplified Polymorphic DNA (RAPD) marker for Encephalartos natalensis. Initially, 140 primers were used to amplify the bulk DNA of five individuals each of known male and female sexuality. While a high degree of polymorphism was observed in the amplification profiles of male and female plants, only primer OPD-20 generated a specific band (∼850 bp) in female DNA. To validate this observation, this primer was re-tested with 69 individuals of E. natalensis. The 850 bp DNA band was present in all 38 female individuals tested and it was consistently absent in all 31 male plants tested. The result offers a rapid and simple test to determine sexuality of E. natalensis seedlings at early stages of development, before the onset of reproductive maturity thereby saving time and economic resources when cultivating these specimens.Electronic Supplementary Material Supplementary material is available to authorised users in the online version of this article at . 相似文献
26.
Phlorotannins are secondary metabolites produced by brown seaweeds with antiviral, antibacterial, antifungal, and larvicidal activities. Phlorotannins’ structures are formed by dibenzodioxin, ether and phenyl, ether, or phenyl linkages. The polymerization of phlorotannins is used to classify and characterize. The structural diversity of phlorotannins grows as polymerization increases. They have been characterized extensively with respect to chemical properties and functionality. However, review papers of the biological activities of phlorotannins have focused on their antibacterial and antiviral effects, and reviews of their broad antifungal and larvicidal effects are lacking. Accordingly, evidence for the effectiveness of phlorotannins as antifungal and larvicidal agents is discussed in this review. Online databases (ScienceDirect, PubMed, MEDLINE, and Web of Science) were used to identify relevant articles. In total, 11 articles were retrieved after duplicates were removed and exclusion criteria were applied. Phlorotannins from brown seaweeds show antifungal activity against dermal and plant fungi, and larvicidal activity against mosquitos and marine invertebrate larvae. However, further studies of the biological activity of phlorotannins against fungal and parasitic infections in aquaculture fish, livestock, and companion animals are needed for systematic analyses of their effectiveness. The research described in this review emphasizes the potential applications of phlorotannins as pharmaceutical, functional food, pesticide, and antifouling agents. 相似文献
27.
Surya Sankar Hiron M. Harshan S.R. Somarajan S.K. Srivastava 《Research in veterinary science》2010,88(3):375-378
A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis. 相似文献
28.
免耕对土壤物理性质及作物产量的影响 总被引：5，自引：0，他引：5
在位于黄河流域半干旱区清水河县,设置免耕不同轮作方式对土壤物理性质及作物产量的影响试验。对免耕不同耕作方式下,土壤团粒结构、总孔隙度、水热变化及作物产量进行了测定。结果表明:经3 a的免耕+轮作后,土壤团粒结构,总孔隙度和水分含量均有逐年增加的趋势,其中留茬覆盖处理尤为明显。秸秆覆盖处理土壤团聚体在0.25~0.5 mm之间含量最高;常规耕作与留茬不覆盖在0.1~0.25 mm之间达到最高值。说明,留茬覆盖有利于形成大粒径的团聚体颗粒,留茬不覆盖处理对于土壤微结构的形成有良好的促进作用。同时,土壤总孔隙度在不同年际间均表现为留高茬覆盖留低茬覆盖留高茬留低茬常规耕作,可见,免耕不同轮作方式对土壤的改善有一定的积极作用。实施免耕的前2 a,作物产量不稳定,甚至造成减产,第3年免耕增产效应有所显现。 相似文献
29.
G Gale Wagner Patricia Holman Surya Waghela 《Veterinary Clinics of North America: Food Animal Practice》2002,18(3):417-30, vi-vii
Suppose one of your clients from southern Florida starts talking about cattle egrets while you are vaccinating her cat. It seems she found a nearly dead egret near the cattle pen a few days ago, picked it up, and noticed a number of what looked like small ticks on the legs. Or, suppose you are called out to a small dairy in central Texas to look at some cows that are feverish and anemic. The first animal you examine has a few brown ticks attached just under the tail. Finally, perhaps you are looking at a lame tortoise for a reptile fancier, a new client, and find a large, colorful tick on a hind leg, well up under the shell. Ring any bells? Egrets are great hosts for the immature stages of Amblyomma ticks and have been captured and marked in the eastern Caribbean, then recaptured in the Florida Keys. Those cattle ticks in Texas might be acaricide-resistant Boophilus ticks that originated in Mexico. The Amblyomma tick on the tortoise could well have "hitch-hiked" all the way from South Africa. By now you remember that both Amblyomma and Boophilus ticks are efficient vectors of two tickborne diseases in this hemisphere, heartwater (in the case of Amblyomma) and babesiosis (transmitted by Boophilus ticks). Both of these diseases are exotic to the United States, and because our livestock are considered to be totally susceptible, an introduced infection could result in high initial death losses (approximately 70%); thus, both the ticks and the diseases pose immediate threats to the health and economic security of United States animal industries. Most importantly, you, whether as a small animal or large animal practitioner, are the first line of defense against such exotic diseases and their vectors. 相似文献