Yeast cells provide insight into alpha-synuclein biology and pathobiology     

Outeiro TF  Lindquist S 《Science (New York, N.Y.)》2003,302(5651):1772-1775

Alpha-synuclein is implicated in several neurodegenerative disorders, such as Parkinson's disease and multiple system atrophy, yet its functions remain obscure. When expressed in yeast, alpha-synuclein associated with the plasma membrane in a highly selective manner, before forming cytoplasmic inclusions through a concentration-dependent, nucleated process. Alpha-synuclein inhibited phospholipase D, induced lipid droplet accumulation, and affected vesicle trafficking. This readily manipulable system provides an opportunity to dissect the molecular pathways underlying normal alpha-synuclein biology and the pathogenic consequences of its misfolding.  相似文献   

Control of regulatory T cell development by the transcription factor Foxp3   总被引：5，自引：0，他引：5  

Hori S  Nomura T  Sakaguchi S 《Science (New York, N.Y.)》2003,299(5609):1057-1061

  相似文献   

Reversible control of hydrogenation of a single molecule     

Katano S  Kim Y  Hori M  Trenary M  Kawai M 《Science (New York, N.Y.)》2007,316(5833):1883-1886

Low-temperature scanning tunneling microscopy was used to selectively break the N-H bond of a methylaminocarbyne (CNHCH3) molecule on a Pt(111) surface at 4.7 kelvin, leaving the C-H bonds intact, to form an adsorbed methylisocyanide molecule (CNCH3). The methylisocyanide product was identified through comparison of its vibrational spectrum with that of directly adsorbed methylisocyanide as measured with inelastic electron tunneling spectroscopy. The CNHCH3 could be regenerated in situ by exposure to hydrogen at room temperature. The combination of tip-induced dehydrogenation with thermodynamically driven hydrogenation allows a completely reversible chemical cycle to be established at the single-molecule level in this system. By tailoring the pulse conditions, irreversible dissociation entailing cleavage of both the C-H and N-H bonds can also be demonstrated.  相似文献   

Plasma LH, Ovulation and Conception Rates in Cats Mated Once or Three Times on Different Days of Oestrus     

T Tsutsui  C Higuchi  M Soeta  H Oba  T Mizutani  T Hori 《Reproduction in domestic animals》2009,44(S2):76-78

Although cats are induced ovulators, the relationship between the day of breeding, the number of matings and the likelihood of ovulation and conception have not been extensively investigated. In this experiment, cats were mated either once or three times on day 1 or day 5 of oestrus to study the incidence of the LH surge, ovulation and conception rates. The percentage ovulating and the conception rates after a single mating on day 1 of oestrus were 60% (6/10) and 33.3% (2/6), respectively, and for cats mated once on day 5 of oestrus were 83.3% (10/12) and 40% (4/10), respectively. When cats were mated three times on day 1 of oestrus, the ovulation rates and conception rates were 70% (7/10) and 85.7% (6/7), respectively, and for those mated three times on day 5 of oestrus were 100% (10/10) and 100% (10/10), respectively. The concentration of LH did not increase in non-ovulating cats, and cats that were mated three times had LH concentrations that were numerically higher than those that were mated once. Litter size was neither related to the day of mating nor to the number of matings. Although an increase in the number of matings on day 1 of oestrus produced a numerically larger LH surge, it did not increase the ovulation rate, suggesting that plasma oestradiol concentrations were not sufficiently elevated to induce a high pituitary response to mating stimulation. The conception rate after a single mating was low, suggesting that the number of sperm per mating was not sufficient. These results suggest that mating more than once in the middle of oestrus is required to improve ovulation rates and conception rates in cats.  相似文献   

Occurrence of a temperate coastal flatfish,the marbled flounder Pseudopleuronectes yokohamae,at high water temperatures in a shallow bay in summer detected by acoustic telemetry     

Mitamura  Hiromichi  Arai  Nobuaki  Hori  Masakazu  Uchida  Keiichi  Kajiyama  Makoto  Ishii  Mitsuhiro 《Fisheries Science》2020,86(1):77-85

Fisheries Science - The marbled flounder Pseudopleuronectes yokohamae, a flatfish found in temperate coastal waters, is an important commercial species in the northwestern Pacific Ocean, but little...  相似文献   

Bursatella leachii from Mar Menor as a Source of Bioactive Molecules: Preliminary Evaluation of the Nutritional Profile,In Vitro Biological Activities,and Fatty Acids Contents     

Tiago Braga  Maria João Rodrigues  Hugo Pereira  João Varela  Luísa Barreira  Mercedes González-Wangüemert 《Journal Of Aquatic Food Product Technology》2017,26(10):1337-1350

Marine invertebrates contain nutritionally important elements that make them suitable to be included in the human diet, and they are considered an outstanding source of secondary metabolites with multiple biotechnological uses. The sea hare Bursatella leachii is an invasive species in Mar Menor that can be managed through the control of its reproduction and/or by massive biomass removal. In this context, this work evaluated the possible use of biomass from B. leachii in the food and cosmetics industries. Biomass was appraised for nutritional properties, and extracts were prepared and evaluated in vitro for antioxidant and anti-inflammatory capacity, cholinesterase and tyrosinase inhibition, and for total contents in different phenolic groups. It had a moderate moisture level, low ash and fat levels, and an adequate protein amount and protein/lipid ratio. The fatty acids (FA) profile was mainly saturated, dominated by palmitic acid. The methanol extract had the highest acetylcholinesterase inhibition. The acetone extract had the strongest capacity to scavenge 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical, reduce iron, inhibit tyrosinase, decrease nitric oxide production in lipopolysaccharide (LPS)-stimulated microglia cells, and the upmost levels of total phenolics, flavonoids, and tannins. Our results suggest multiple biotechnological applications for B. leachii biomass in different economic areas.  相似文献   

Cross‐Linking of Wheat Gluten Using a Water‐Soluble Carbodiimide     

V. Tropini  J.‐P. Lens  W. J. Mulder  F. Silvestre 《Cereal Chemistry》2000,77(3):333-338

Wheat gluten was cross‐linked using water‐soluble 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide HCl (EDC). To enhance cross‐linking, N‐hydroxysuccinimide (NHS) was added to the reaction mixture. The cross‐linking efficiency was evaluated by the decrease in the amount of amino groups, the solubility of the protein in aqueous solutions with different pH levels, and by the change in the molecular weight distribution of the cross‐linked compounds. Cross‐linking was dependent on the reaction time, the molar ratio of added reactants, and the pH level of the reaction mixture. If the reaction was carried out at pH 3, no decrease in the amount of amino groups or solubility was observed. At pH 5–7, the amount of amino groups decreased from 15 to 10 mmol/100 g of protein. This was accompanied by a large decrease in the water solubility of the protein (<10%, w/v). Finally, reaction at pH 11 decreased the amount of amino groups from 15 to 8 mmol/100 g of protein. However, hardly any decrease in the water solubility was observed. Based on these results and SDS‐PAGE experiments, two cross‐link mechanisms are suggested: one resulting in inter‐ and the other resulting in intramolecular cross‐links.  相似文献   

Seaweed Blends as a Valuable Source of Polyunsaturated and Healthy Fats for Nutritional and Food Applications     

Francisca Marques  Diana Lopes  Elisabete da Costa  Tiago Conde  Andreia Rego  Ana Isabel Ribeiro  Maria Helena Abreu  Maria Rosrio Domingues 《Marine drugs》2021,19(12)

Seaweeds are considered healthy and sustainable food. Although their consumption is modest in Western countries, the demand for seaweed in food markets is increasing in Europe. Each seaweed species has unique nutritional and functional features. The preparation of blends, obtained by mixing several seaweeds species, allows the obtaining of maximum benefits and ingredients with single characteristics. In this work, five seaweed blends, commercially available and produced under organic conditions in Europe, were characterized. The proximal composition included contents of ash (20.28–28.68% DW), proteins (17.79–26.61% DW), lipids (0.55–1.50% DW), and total carbohydrates (39.47–47.37% DW). Fatty acid profiles were determined by gas chromatography–mass spectrometry (GC–MS), allowing quantification of healthy fatty acids, namely n-3 and n-6 polyunsaturated fatty acids (PUFA), and calculation of lipid quality indices. Each blend showed a characteristic PUFA content in the lipid pool (35.77–49.43% of total fatty acids) and the content in essential and healthy n-3 PUFA is highlighted. The atherogenicity (0.54–0.72) and thrombogenicity (0.23–0.45) indices evidenced a good nutritional value of lipid fractions. As nutritional and environmentally attractive products, the consumption of the studied seaweed blends can contribute to a healthy lifestyle.  相似文献   

Soybean expansion impacts on soil organic matter in the eastern region of the Maranhão State (Northeastern Brazil)     

Marcos Siqueira-Neto  Gustavo V. Popin  Gregori E. Ferrão  Arthur K. B. Santos  Carlos E. P. Cerri  Tiago O. Ferreira 《Soil Use and Management》2022,38(2):1203-1216

Recently, the eastern region of the Maranhão state (Northeastern Brazil) became a hotspot of land-use change (LUC) directly from native vegetation to soybean cultivation, but due to the soil characteristics, LUC has caused substantial soil organic carbon (SOC) and nitrogen depletion. Therefore, we quantified these impacts arising from two factors: (i) different timeframes after LUC and (ii) contrasting soil management practices. For the first study, soil samples (0–30 cm; six replicates) were taken on soybean fields year one, year eight and 15 years after LUC. It the second study, another area was sampled, of which part was managed under no-tillage (NT) and the other using a mouldboard plough (MP). For both studies, native vegetation (NV) was sampled as the control. NV stored about 50 Mg of carbon (C) ha⁻¹; but LUC reduced C stocks by 35% (after 8 and 15 years); moreover, labile-C decreased between 20% and 45%, while, microbial-C decreased between 20% and 60%, considering the interval between year one and 15 years. Regarding soil management, the MP did not cause differences on C stock (24 Mg C ha⁻¹) in comparison to NT; however, both labile-C and microbial-C decreased by 15% to NT, while, decreased by 40% to MP. These results lead us to believe that, since LUC is inevitable, we suggested the adoption of the best agricultural management practices, in order to preserve/increase the SOC, reducing the impacts on GHG emissions and, thus, achieving sustainability and profitability.  相似文献   

Effect of water deficit on morphoagronomic traits of black common bean genotypes (Phaseolus vulgaris L.) with contrasting drought tolerance     

Sebastião Soares de Oliveira Neto  Francisca Franciana Sousa Pereira  Tiago Zoz  Carlos Eduardo da Silva Oliveira  Vânia Moda Cirino 《Journal of Agronomy and Crop Science》2023,209(1):83-93

Brazil is the world's largest producer of common beans (Phaseolus vulgaris L.). Drought stress harms the morphological and agronomic traits of beans. This study evaluates the reaction to water deficit in five genotypes of black beans. The experiment was conducted in the IDR-IAPAR-EMATER in Londrina-PR, Brazil. A split-plot design was used, with three replications. The genotypes were included in the subplots and the treatments with or without water deficit in the plots. Water deficit was induced on the pre-flowering stage and maintained for 20 days in the plots submitted to drought stress. For the growth analysis, plants were collected at 35, 54 and 70 days after emergence. At the stage of physiological ripeness, several morphological and yield traits were evaluated. The genotypes IPR Uirapuru and BRS Esplendor can be considered tolerant and used as a tolerant source to water deficit in common bean germplasm banks. The line LP 08-90 has morphological and agronomic adaptations efficient to overcome water deficit's effects, presenting a higher grain yield in both crop conditions, which indicates the success of black beans breeding to deal with water deficit.  相似文献