Thirty years of gap dynamics in a central european beech forest reserve     

Kenderes  Kata; Mihok  Barbara; Standovar  Tibor 《Forestry》2008,81(1):111-123

Investigation of gap characteristics and tree regeneration patternsis central for our understanding of forest dynamics. By integratingaerial photograph analyses and ground surveys, we provide astudy of long-term canopy gap dynamics and tree regenerationpatterns in a Hungarian beech forest reserve. We found (1) thatin spite of the overall increase of gap area during the investigated30-years (from 2.5 to 7.7 per cent), total gap area and averagegap size (40–93 m²) were remarkably similar to those foundin other temperate and tropical forests, (2) if the fate ofindividual gaps was followed, two to three times more intensivecanopy dynamics (gap creation, closure and expansion) couldbe recognized than simple change of gap area indicated, and(3) average seedling density was considered to be sufficientfor natural regeneration. However, it was apparent that recentincreased deer browsing had prevented establishment of youngertrees of 1–2 m in height, as taller saplings were recordedonly in old gaps. Our results not only provide useful informationon forest dynamics but can also contribute to understandingthe potential roles that small forest reserves can play in providingessential reference data for nature-based forest managementof this forest type.  相似文献   

Alphaproteobacteria communities depend more on soil types than land managements     

Orsolya Gazdag  Tünde Takács  László Ködöböcz  Gergely Krett  Tibor Szili-Kovács 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2019,69(2):147-154

Soil properties and agricultural practices take a joint effect on the communities of soil bacteria. The aim of the present study was to survey Alphaproteobacterial communities as possible indicators of soil quality considering clay, loamy and sandy soils under conventional and organic farming. Alphaproteobacteria community composition were analysed by 16S rRNA gene with nested-PCR (polymerase chain reaction) and denaturing gradient gelelectrophoresis (DGGE). Sequencing of partial 16S rRNA gene from the DGGE bands were performed. Conventional and organic farming resulted in significant differences in chemical properties of soils. According to the results community fingerprints were separated into groups depending on soil types and farming systems. This separation can be attributed mostly to soil pH, AL-P₂O₅,-K₂O. The analysed sequences were identified as soil bacteria which could play the main role in nitrogen fixing, mineralisation and denitrification. The highest diversity index was revealed from the organic farming at sandy texture site, where mainly Mesorhizobium sp. and Rhizobium sp. were detected. The soil type and actual crop could have a stronger impact on the soil bacterial composition than the management.  相似文献   

Soil salinity management in agriculture: Technological advances and applications (Innovations in biological engineering),     

Gyöngyi Barna  Tibor Tóth 《Arid Land Research and Management》2017,31(4):485-487

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Genetic Variability of Central European Isolates of the <Emphasis Type="Italic">Fusarium graminearum</Emphasis> Species Complex     

Beáta?TóthEmail author  ákos?Mesterházy  Zoltán?Horváth  Tibor?Bartók  Mónika?Varga  János?Varga 《European journal of plant pathology / European Foundation for Plant Pathology》2005,113(1):35-45

The main causative agents of Fusarium head blight in central Europe are Fusarium graminearum and F. culmorum. We examined the mycotoxin producing ability, aggressiveness and molecular variability of F. graminearum isolates. Altogether twenty-six Hungarian, three Austrian isolates and representatives of eight species identified in the F. graminearum species complex were involved in this study. Mycotoxin producing abilities of the isolates were tested by GC-MS and HPLC. The central European isolates were found to belong to chemotype I (producing deoxynivalenol). Most isolates produced more 15-acetyl-deoxynivalenol than 3-acetyl-deoxynivalenol suggesting that they belong to chemotype Ib. All F. graminearum isolates were found to be highly pathogenic in in vitro aggressiveness tests. Phylogenetic analysis of random amplified polymorphic DNA profiles, and restriction profiles of the intergenic spacer region of the ribosomal RNA gene cluster of the isolates allowed clustering of the central European isolates into 17 and 16 haplotypes, respectively. When RAPD and IGS-RFLP data were combined, almost every single central European F. graminearum isolate could be differentiated (27/29 haplotypes). Sequence analysis of a putative reductase gene of some isolates was also performed. Based on molecular data, the majority of the central European isolates belonged to F. graminearum sensu stricto characteristic to the northern hemisphere, with the exception of one Hungarian isolate, which was not related to any known species of the F. graminearum species complex based on sequence data. The taxonomic assignment of two other Hungarian isolates, previously suggested as belonging to F. boothii based on mitochondrial DNA restriction profiles, was supported by sequence analysis.  相似文献   

Ixodid tick species attaching to dogs in Hungary     

Földvári G  Farkas R 《Veterinary parasitology》2005,129(1-2):125-131

A survey was carried out to investigate the occurrence of hard tick species (Acari: Ixodidae) infesting domestic dogs in Hungary. Forty veterinary clinics from a wide geographical area were asked to collect hard ticks from dogs and to complete a questionnaire. In total, 25 veterinary clinics submitted 900 ticks from 310 dogs. Intensity of infestation ranged from one to 78 per dog. The most preferred sites of tick attachment in decreasing order were head, neck and legs. The majority of ticks (91.7%) were adults, which were identified to species level, the others were nymphs. Six species were found: Dermacentor reticulatus (48.9%), Ixodes ricinus (43.2%), Ixodes canisuga (5.6%), Haemaphysalis concinna (2%) and there was one specimen of both Dermacentor marginatus and Ixodes hexagonus. Single species infestation with I. ricinus or D. reticulatus was found on 145 (46.8%) and 120 animals (38.7%), respectively. Mixed infestation caused by these two species was detected on 24 dogs (7.7%). I. canisuga and H. concinna were found on seven and five dogs, respectively. D. reticulatus and I. ricinus were collected almost throughout the year, except for a single month. The activity peaks were in spring and in autumn for both species. Based on clinical signs, canine babesiosis was diagnosed by the veterinarians in 66 (21.3%) tick infested dogs. These dogs were more frequently infested with D. reticulatus than the others. Our data contribute to the understanding of geographical and seasonal distribution of ixodid tick species infesting dogs in Hungary. The implication of these data, for the risk of canine tick borne diseases is discussed.  相似文献   

Study on the transfer of components of Aetheroleum carvi and Aetheroleum foeniculi oils     

Lado C  Hajdú M  Farkas E  Then M  Taba G  Szentmihályi K 《Fitoterapia》2005,76(2):166-172

The transfer through a membrane of the main organic components and mineral elements of Carum carvi and Foeniculum vulgare volatile oils was studied. The transfer was studied from buffer solution pH 1.1 (stomach) and pH 6.5 (intestine) to pH 7.5 (plasma) in a Sartorius model. The transferred components were measured by GC-MS for volatile components and ICP-OES for inorganic elements. The main components (trans-anethole and fenchone from F. vulgare, carvone and D-limonene from C. carvi), as well as some mineral elements (Ca, Mg, S, Zn), transferred through the membrane.  相似文献   

First detection of small babesiae in two dogs in Hungary     

Farkas R  Földvári G  Fenyves B  Kótai I  Szilágyi A  Hegedüs GT 《The Veterinary record》2004,154(6):176-178

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How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing   总被引：1，自引：0，他引：1  

Godfroid J  Saegerman C  Wellemans V  Walravens K  Letesson JJ  Tibor A  Mc Millan A  Spencer S  Sanna M  Bakker D  Pouillot R  Garin-Bastuji B 《Veterinary microbiology》2002,90(1-4):461-477

Collaborative work was financed by the EU to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to Yersinia enterocolitica O:9 either in conjunction with, or as an alternative to, the classical serological, bacteriological or allergic skin tests. Sixteen heifers were experimentally infected with Brucella abortus biovar 1 (five heifers), Brucella suis biovar 2 (two heifers), Y. enterocolitica O:9 (six heifers) and Y. enterocolitica O:3 (three heifers). Four heifers, naturally infected with Y. enterocolitica O:9 that presented aspecific brucellosis serological reactions were also included in the experiment. A self-limited infection was induced in cattle by B. suis biovar 2. All the brucellosis serological tests used, i.e. the slow agglutination test (SAW), the Rose Bengal test (RB), the complement fixation test (CFT), indirect and competitive ELISA’s, lacked specificity when used to analyze sera from Y. enterocolitica O:9 infected animals. A Yersinia outer membrane proteins (YOPs)-ELISA was also used and although the test is able to detect a Yersinia group infection, it provided no evidence of whether or not there is a possible brucellosis infection when dual infections are present. The brucellergen IFN-γ test showed a lack of specificity also. The only test that was proven to be specific is the brucellergen skin test. All brucellosis serological tests, except the indirect ELISA, were limited in their ability to detect B. abortus persistently infected animals.

Based on these experimental studies, a strategy was implemented as part of the year 2001 Belgian Brucellosis Eradication Program to substantiate the eradication of bovine brucellosis. Epidemiological inquiries have identified risk factors associated with aspecific serological reactions, possible transmission and infection of cattle by B. suis biovar 2 from infected wild boars; and both legal and administrative measures taken by the veterinary services. No cases of bovine brucellosis have been confirmed in Belgium since March 2000.  相似文献   

Subarachnoid pressures and cardiorespiratory parameters during cisternal myelography in isoflurane anaesthetized dogs     

Attila Arany‐Tóth  Péter Csébi  Jenő Reiczigel  Viktoria Sére  Tibor Németh 《Veterinary anaesthesia and analgesia》2013,40(4):390-398

ObjectiveTo measure subarachnoid pressures, systemic circulatory and respiratory effects, and to calculate cerebral perfusion pressure during cisternal myelography.Study designProspective clinical study.AnimalsForty‐three client owned dogs with clinical signs of spinal disease, weighing 6–56 kg.MethodsDogs were premedicated with butorphanol and diazepam intravenously (IV) and anaesthesia was induced with propofol and maintained with isoflurane vaporized in oxygen. Ventilation was spontaneous. Heart and respiratory rates, invasive mean arterial blood pressure (MAP), end tidal carbon dioxide and isoflurane concentration were measured continuously. Initial subarachnoid pressure (SaP₀) was measured in the cisterna magna with a needle pressure gauge. Iohexol 0.3 mL kg^?1 was injected at a rate of 4.1 mL minute^?1 into the cerebellomedullary cistern. The SaP was recorded during and at 120 seconds after contrast administration. The maximum SaP (SaP_max) and minimum calculated cerebral perfusion pressure (CPP_min) were recorded for each case.ResultsPrior to contrast injection, mean ± SD, MAP was 73 ± 20 mmHg and SaP₀ was 10 ± 3 mmHg. The cerebral perfusion pressure (CPP) was 64 ± 20 mmHg. The contrast injection increased the SaP₀ to 73 ± 33 mmHg (SaP_max). After injection, MAP increased to 97 ± 25 mmHg and the CPP decreased to 14 ± 34 mmHg. A negative correlation was found between the lowest CPP and body weight (ρ = ?0.77, p < 0.0001). Nine dogs had bradycardia, apnoea and hypertension, 21 dogs had at least one of these signs. The number of clinical signs showed significant correlation with body weight (ρ = ?0.68, p < 0.0001), SaP_max (ρ = ?0.66, p < 0.0001) and CPP_min (ρ = ?0.73, p < 0.0001).Conclusions and clinical relevanceCerebral perfusion can severely decrease during cisternal myelography using the standard dose of iohexol. Bradycardia, apnoea and systemic hypertension were associated with decreased CPP.  相似文献   

CT measures of osseous cervicothoracic intervertebral foramina are repeatable and associated with CT measures of adjacent articular processes in horses     

Tibor Rovel  Luc Duchateau  Jimmy Saunders  Filip Vandenberghe  Katrien Vanderperren 《Veterinary radiology & ultrasound》2023,64(1):61-68

Narrowing of the equine cervicothoracic intervertebral foramina (IF) has the potential to cause forelimb lameness and/or neck pain although limited information is available on CT of the IF. The aims of this retrospective, analytical study were to describe a protocol for quantifying CT cervicothoracic IF size; evaluate the repeatability of IF size measures; test associations between IF size and adjacent articular process (AP) size, ventral extent, and anatomic location; and determine the proportion of IF with narrowing. Computed tomographic images were acquired in 20 Warmblood horses that presented with forelimb lameness and/or neck pain. All IF between C5 and T2 (n = 160) were evaluated. IF cross-sectional area (CSA), APCSA, and AP ventral extent were measured. The repeatability of IFCSA measurement was calculated. Possible associations between IFCSA and: APCSA, ventral extent, side, or location were assessed. IF narrowing was defined as more than 50% of reduction in IF height when compared with its widest part(s). The repeatability of IFCSA measurement was excellent. There was a significant association between IFCSA and: APCSA (P < 0.001; R² = 0.859; slope = −0.106), ventral extent (P = 0.022; R² = 0.161; slope = −0.0617), and location (P < 0.001; higher values between C7 and T2). The association between IFCSA and ventral extent was small. Narrowing was identified in 61 (38.1%) IF. Maximum degree of narrowing was most common at the cranial (26.3%) and middle (68.8%) third of the IF. Narrowing was not identified at T1-T2. In conclusion, CT cervicothoracic IF size can be measured with excellent repeatability, and associations were found between IF size and: AP size, ventral extent, and location.  相似文献