Immune responses of orange-spotted grouper,Epinephelus coioides,against virus-like particles of betanodavirus produced in Escherichia coli     

Yu-Xiong Lai  Bao-Lei Jin  Yu Xu  Li-jie Huang  Run-Qing Huang  Yong Zhang  Jimmy Kwang  Jian-Guo He  Jun-Feng Xie 《Veterinary immunology and immunopathology》2014,157(1-2):87-96

Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a serious disease of cultured marine fish worldwide. Virus-like particles (VLPs) are one of the good novel vaccine candidates to control this disease. Until now, betanodavirus vaccine studies mainly focused on the humoral immune response and mortality after virus challenge. However, little is known about the activation of genes responsible for cellular and innate immunity by vaccines. In the present study, VLPs of orange-spotted grouper nervous necrosis virus (OGNNV) were produced in prokaryotes and their ability to enter Asian sea bass cells was the same as native virus, suggesting that they possess a similar structure to OGNNV. VLPs immunogenicity was then determined by intramuscularly vaccinating Epinephelus coioides at different concentrations (1.5 or 15 μg g^?1 fish body weight, FBW) and immunizing frequencies (administration once, twice and thrice). A single vaccination with the dosage of 1.5 μg g^?1 FBW is enough to provoke high titer antibodies (average 3 fold higher than that of negative control) with strong neutralizing antibody titer as early as 1 week post immunization. Furthermore, quantitative PCR analysis revealed that eleven genes associated with humoral, cellular and innate immunities were up-regulated in the liver, spleen and head kidney at 12 h post immunization, correlating with the early antibody response. In conclusion, we demonstrated that VLP vaccination induced humoral immune responses and activated genes associated with cellular and innate immunity against betanodavirus infection in orange-spotted grouper.  相似文献   

IL-10 Gene Polymorphisms and Their Association with Immune Traits in Four Rabbit Populations     

Xiaoying WAN  Liuliu MAO  Ting LI  Lizhi QIN  Yulai PAN  Bichun LI  Xinsheng WU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):369-375

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Impact of Food Restriction on Ovarian Development,RFamide‐Related Peptide‐3 and the Hypothalamic–Pituitary–Ovarian Axis in Pre‐Pubertal Ewes     

H Li  H Song  M Huang  H Nie  Z Wang  F Wang 《Reproduction in domestic animals》2014,49(5):831-838

RFamide‐related peptide‐3 (RFRP‐3), the mammalian ortholog of gonadotropin‐inhibiting hormone, has been implicated as a mediator between reproduction and energy balance. This study aimed to investigate the physiological effects of RFRP‐3 on the process of ovarian development in food‐restricted pre‐pubertal ewes. The results showed that food restriction significantly inhibited the ovarian development and follicular growth. The data of qPCR in the hypothalamic–pituitary–ovarian (HPO) axis showed that food restriction not only upregulated RFRP‐3 mRNA expression but also downregulated the mRNA expression of gonadotropin‐releasing‐hormone receptor, follicle‐stimulating hormone receptor and luteinizing hormone receptor (LHR). Immunohistochemistry of RFRP‐3 in the ovaries suggested that RFRP‐3 may regulate the follicular development. These results suggested that the changes of RFRP‐3 in response to food restriction might influence the HPO axis and inhibit ovarian development.  相似文献   

红嘴蓝鹊的巢址选择     

于学伟  王福云  江志  黄淑芳  胡新波 《野生动物》2014,(4):440-444

2011 ～2013年2～7月,对红嘴蓝鹊（Urocissa erythrorhyncha）的巢址选择进行了研究,结果表明：红嘴蓝鹊营巢最早开始于2月下旬,结束于6月下旬;第一窝出飞后,即开始另选巢址进行第二窝繁殖,原巢不再利用.红嘴蓝鹊营巢于树上,选择2～4月建巢,均为常绿树种;5～6月建巢既有常绿树种,也有落叶树种.巢树平均高度（11.12 ±4.15）m、胸径（26.23±12.85）cm、巢高（7.35±2.80）m （n=13）,主成分分析表明,影响红嘴蓝鹊巢址选择的主要因子有3个,依次为：巢树因子（主要包括：树高、树胸径、巢高）、隐蔽与食物水源因子（巢上郁闭度、巢距最近食源地距离、巢距最近水源距离）、光与人类活动影响因子（巢与巢枝朝向、巢距道路距离）.繁殖期红嘴蓝鹊对人为干扰与噪音敏感,巢位较低和较大的暴风雨等均可能造成红嘴蓝鹊弃巢.  相似文献   

对虾养殖技术之一日本对虾围塘精养高产技术     

周晨光  陈佳颖  黄秀癸 《中国水产》2006,369(8)

日本对虾在我国已有多年的养殖历史,以前多采用围塘与贝类等混养的方式,亩产只有20kg-25kg左右,养殖产量一直没有大的突破。2005年,舟山市绿源水产养殖公司采用围塘进行日本对虾精养高产技术研究,养殖亩产量达到366．87kg,经济效益十分显著。  相似文献   

AFLP技术在水生动物遗传学研究中的应用     

曹廷  陈婵娟  庞璐  黄亚红 《水产科学》2007,26(11):637-639

AFLP(Amp lifed Fragm ent Length P loymorphsim)技术是由荷兰Keygene公司科学家Zabeau和Vos发展起来的一种检测DNA多态性的一种方法[1],1993年获欧洲专利局专利,该方法是继RFLP(Restriction Fragm ent Length P loymor-phsim),SSR(S imp le Sequence Repeat)和RAPD(RandomAmp lified P loymorphsim DNA)之后发展最快的DNA指纹技术,是DNA指纹技术的重大突破,也是目前国际上最新最适用的DNA指纹技术。AFLP结合了RFLP和RAPD的特点,它兼有RFLP技术的可靠性和PCR技术的高效性,且具有快速、灵敏、稳定、所需DNA量少…  相似文献   

浅谈当前集体林采伐管理存在问题和对策   总被引：1，自引：0，他引：1  

黄水平 《林业科技情报》2014,46(4)

通过分析林业产权制度改革后新形势下集体林采伐管理存在的问题,提出了今后进一步改革和完善集体林采伐管理的对策。  相似文献   

河北省钢铁产业现状及发展趋势研究     

黄少琴 《绿色科技》2014,(8):297-300

从河北钢铁产业新增产能、产量、经济效益、钢材进出口、行业集中度、节能减排等角度探讨了河北钢铁产业的现状,并对河北钢铁产业进行了SWOT分析;从产业布局、合并重组、专业化分工、钢铁企业的发展趋势等角度探讨了河北钢铁产业未来的发展趋势。  相似文献   

使樱花赏花期延长的日本引种品种的选育搭配研究     

况红玲  王燕  黄冬  王昆  瞿燕群  胡芳 《绿色科技》2014,(10)

对武汉法雅园林集团有限公司从日本引进的39个樱花品种的成活率和花期进行了观测研究,筛选出了23个成活率在80%以上的花期不同的品种,为园林绿化工程中延长樱花赏花期的品种搭配设计提供参考依据。  相似文献   

菠萝叶纤维与其它植物纤维的吸湿性能对比测试及分析     

连文伟  张劲  薛忠  李明福  黄涛 《绿色科技》2014,(10)

利用扫描电镜、X射线衍射等测试方法对菠萝叶纤维、亚麻纤维、苎麻纤维和棉花纤维的微观形态结构进行了分析。分析认为:菠萝叶纤维的形态结构有区别于其它三种植物纤维,有较大的比表面积;菠萝叶纤维的结晶度与亚麻纤维和苎麻纤维接近,高于棉花纤维。在热带标准状态下,采用烘箱干燥法和回归模型分析法,对测试的四种进行了吸湿、放湿测试与分析。结果表明:在吸湿性能方面,菠萝叶纤维表现最佳,棉花纤维最差;在纤维吸湿平衡回潮率和吸湿速率方面,菠萝叶纤维与亚麻纤维和苎麻纤维相近,且高于棉花纤维;在纤维放湿平衡回潮率和放湿速率方面,菠萝叶纤维高于亚麻纤维、苎麻纤维和棉花纤维。  相似文献