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排序方式: 共有172条查询结果,搜索用时 15 毫秒
21.
The Northern Pacific sea star Asterias amurensis has a major negative impact on scallop mariculture. In northern Japan, fishermen clean up sea stars before releasing young scallops in the mariculture field; however, new sea stars constantly invade the field from outside areas to feed on scallops. Thus, it is important to determine the migration speed and seasonal behavioral patterns of the Northern Pacific sea star to implement effective density control measures. Here, we set out to quantify these parameters using acoustic telemetry. In a rearing experiment, acoustic transmitters were retained on sea stars for up to 71 days using nylon fishing line. In the field experiment, we showed that the moving distance of the Northern Pacific sea star over a 1-week period was significantly further in spring (90.9 ± 49.9 m) than in summer (25.1 ± 18.9 m), and that the moving speed was significantly faster in spring (18.1 ± 15.2 m/day) than in summer (4.3 ± 9.1 m/day). Our results are the first to present the two-dimensional movement of Northern Pacific sea star individuals in spring and summer. We suggest that sea star extermination practices should be extended beyond the immediate culture area. 相似文献
22.
Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus (CMV) reveals geographical region‐related resistance to CMV in Japan
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H. Takahashi A. Tian S. Miyashita Y. Kanayama S. Ando R. Kormelink 《Plant pathology》2018,67(6):1401-1415
Zea mays has been historically imported to Japan via two independent geographical routes: into southern Japan by trading with Europe in the 16th century and into northern Japan by import from North America in the 19th century. Breeding to genetically improve on quality traits and high yields has led to the current domestic landraces in each region. In a survey of 82 domestic landraces, nine out of 38 landraces originating from southern Japan showed complete immunity to cucumber mosaic virus yellow strain (CMV(Y)) without the formation of necrotic local lesions (NLLs). In contrast, three out of 44 landraces originating from northern Japan developed NLLs, but revealed no systemic spread of the virus. Due to the absence of good documentation on NLL formation in Z. mays, the response of domestic landraces Aso‐1 and Aso‐3, originating from Ibaraki in northern Japan, to a challenge with CMV(Y) and CMV(Ma‐1) was further analysed. Aso‐3 only formed NLL in response to CMV(Y) but not to CMV(Ma‐1). Moreover, in CMV(Y)‐inoculated Aso‐3, virus spread was restricted to the primary infection site and the expression of defence‐related genes was up‐regulated, whereas Aso‐1 became systemically infected with either CMV(Y) or CMV(Ma‐1). The response of Aso‐3 to CMV(Y) was inherited as a single dominant trait. Together, these results pointed towards the induction of hypersensitive response (HR)‐mediated resistance to CMV(Y) in Aso‐3. Although HR‐mediated resistance to viruses has been studied mainly in dicots, the pathosystem CMV–Z. mays may provide a model to investigate HR‐mediated resistance to viruses in monocot plants. 相似文献
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24.
Megumi Yamagishi Ritsuko Hosoda-Yabe Hideki Tamai Miku Konishi Akihiro Imamura Hideharu Ishida Tomio Yabe Hiromune Ando Makoto Kiso 《Marine drugs》2015,13(12):7250-7274
LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling. 相似文献
25.
Fui Fui Ching Yoshizumi Nakagawa Keitaro Kato Osamu Murata Shigeru Miyashita 《Aquaculture Research》2012,43(2):303-310
The effects of delayed first feeding on the survival and growth of tiger grouper, Epinephelus fuscoguttatus (Forsskål 1775), larvae were examined under controlled conditions. The total length, yolk sac volume, oil globule volume, yolk sac absorption time and nutritional transition period (NTP) of the larvae fed at different first times (0, 6, 12, 18 and 24 h after the mouth opening stage; h AMO) were compared. Larval first feed intake was observed at 54 h after hatching (h AH) at 27.5 ± 0.5 °C. The yolk sac was consumed more rapidly with an increase in delayed first feeding and was significantly different among treatments (P<0.05). Larvae first fed at 0 h AMO had the longest yolk sac absorption (72 h AH) and NTP (20 h) times and had the highest survival and growth rates at the end of the experimental period (360 h AH), being significantly higher (P<0.05) than the other treatments. First mortality was observed at 69 h AH, approximately 2 h after point of no return (PNR) occurred. This study suggests that first feeding of tiger grouper larvae should commence at 0 h AMO for enhancement of larval survival and growth. 相似文献
26.
Estimation of habitat suitability for the southern form of the short‐finned pilot whale (Globicephala macrorhynchus) in the North Pacific
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Habitat models were developed from dedicated sighting survey data collected during summers between 1983 and 2006 in the North Pacific Ocean. Our aim was to examine the distribution pattern of the southern form of the short‐finned pilot whale (Globicephala macrorhynchus) in relation to the physical environment. We tested two different types of analytical procedures for habitat estimation: generalized linear models (GLMs) and ecological niche factor analysis (ENFA). The area under the receiver operating characteristic curve and the Boyce index suggested that GLM defined the core habitat well, whereas ENFA estimated the suitable habitat more correctly. These models indicated the core habitat within the subtropical gyre. Among the environmental variables used to construct the habitat models, the temperature at a depth of 200 m contributed most to both GLM and ENFA. This corresponds to the fact that the species mainly feeds on mesopelagic prey and that the axis of the Kuroshio Current, in the northwestern part of the subtropical gyre, is characterized by its temperature at 200 m. Habitat suitability in the coastal waters off southern Japan also correlated with the Kuroshio meander patterns. Thus, the southern form of the short‐finned pilot whale appears to be particularly well adapted to the ecosystem of the North Pacific subtropical gyre. 相似文献
27.
Yohei Kawauchi Masayuki Chimura Takashi Mutoh Masamichi Watanobe Hokuto Shirakawa Kazushi Miyashita 《Fisheries Science》2014,80(2):139-149
We examined factors affecting the vertical distribution of juvenile walleye pollock during “the second critical period” (i.e. the transition period of prey size) in and around Funka Bay, Hokkaido, Japan. During the day, small juveniles (<30 mm) were distributed mainly in shallow, warm, and low-salinity water within the bay, whereas larger ones (≥30 mm) occurred in deeper, cold and saline water. During the night, the large juveniles migrated to shallower depths. Outside the bay, small- and large-sized juveniles were distributed in water masses of similar physical properties during the daytime, whereas during the nighttime, most of the juveniles moved to shallower depth, and some juveniles dived to deeper water mass. Dietary analysis revealed that large juveniles fed on large copepods (≥0.5 mm in body width) more than small juveniles did. The composition of zooplankton in our survey area showed that large-sized copepods often occurred in deeper saline water. From these results, there is a possibility that juvenile pollock inside and outside Funka Bay occupied different water masses preferable for each feeding advantage. 相似文献
28.
We examined growth of northern shrimp Pandalus eous in the Sea of Japan, off western Hokkaido, to improve estimations of catch-at-age for stock assessment. Multiple length frequency analysis based on length frequency data collected by a scientific research vessel was conducted to examine length-at-age in the shrimp population. Multi-normal distributions estimated using maximum likelihood indicated a good fit to length distributions. AIC values and regression analyses revealed annual growth variation and a decreasing trend in the length at several age classes in the shrimp population. We revised the method for estimating catch-at-age from the age-conversion table (ACT), which is a simple method for age determination, to age–length keys (ALK) calculated from the results of multiple length frequency analysis. Abundant year classes caught successively year after year could be more easily identified from the catch-at-age data computed using ALK than by using ACT. Our results suggested not only that the mean size of commercial landings fluctuated based on changes in age composition but also that a decrease in the length-at-age in the population influenced the consistent size decrease of commercial landings. 相似文献
29.
Involvement of a vascular hypersensitive response in quantitative resistance to Ralstonia solanacearum on tomato rootstock cultivar LS‐89
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K. Nakaho S. Seo K. Ookawa Y. Inoue S. Ando Y. Kanayama S. Miyashita H. Takahashi 《Plant pathology》2017,66(1):150-158
Ralstonia solanacearum causes bacterial wilt disease in Solanaceae spp. Expression of the Phytophthora inhibitor protease 1 (PIP1) gene, which encodes a papain‐like extracellular cysteine protease, is induced in R. solanacearum‐inoculated stem tissues of quantitatively resistant tomato cultivar LS‐89, but not in susceptible cultivar Ponderosa. Phytophthora inhibitor protease 1 is closely related to Rcr3, which is required for the Cf‐2‐mediated hypersensitive response (HR) to the leaf mould fungus Cladosporium fulvum and manifestation of HR cell death. However, up‐regulation of PIP1 in R. solanacearum‐inoculated LS‐89 stems was not accompanied by visible HR cell death. Nevertheless, upon electron microscopic examination of inoculated stem tissues of resistant cultivar LS‐89, several aggregated materials associated with HR cell death were observed in xylem parenchyma and pith cells surrounding xylem vessels. In addition, the accumulation of electron‐dense substances was observed within the xylem vessel lumen of inoculated stems. Moreover, when the leaves of LS‐89 or Ponderosa were infiltrated with 106 cells mL?1 R. solanacearum, cell death appeared in LS‐89 at 18 and 24 h after infiltration. The proliferation of bacteria in the infiltrated leaf tissues of LS‐89 was suppressed to approximately 10–30% of that in Ponderosa, and expression of the defence‐related gene PR‐2 and HR marker gene hsr203J was induced in the infiltrated tissues. These results indicated that the response of LS‐89 is a true HR, and induction of vascular HR in xylem parenchyma and pith cells surrounding xylem vessels seems to be associated with quantitative resistance of LS‐89 to R. solanacearum. 相似文献
30.
Hobley J Gorelik S Oori T Kajimoto S Fukumura H Matsui J Miyashita T 《Biointerphases》2010,5(3):FA105-FA109
Two-dimensional condensation was initiated in a self-assembled mixed monolayer of spiropyran and octadecanol by a nanosecond laser pulse. The dynamics of the process were monitored using nanosecond pump-probe Brewster angle microscopy. Domain growth followed a power law with a growth exponent of 0.47 at a velocity approaching 20?ms(-1). This represents a limit for the rate of longitudinal signaling of pressure waves through a self-assembled amphiphilic layer at an interface and adds to our understanding of signal transmission rates in biomimetic membranes where morphological change in one region can be signaled to a more remote region. 相似文献