Development of enzyme immunoassay for chromogranin A (CgA) and profiling of plasma CgA concentrations in the cow     

Chihiro Saito  Haruka Ido-Matsumoto  Hiromasa Kumazaki  Tomoaki Kubo  Tomomi Kanazawa  Toru Takahashi 《Animal Science Journal》2021,92(1):e13542

The objectives of this study were to establish and characterize a homologous immunoassay for bovine chromogranin A (bCgA) and to profile plasma bCgA concentrations during early pregnancies. We synthesized oligopeptide corresponding to the amino acid sequence 341–355 of bCgA for immunizing rabbits and peptide corresponding to the amino acid sequence 336–365 of bCgA for both a biotinylated tracer and reference standards. Recombinant bCgA protein was also generated in Escherichia coli lysate. Dose-dependent displacement curves were obtained from 1 to 1,000 nM of the reference standards. The displacement curves showed a good relationship between the reference standards of the synthetic peptide and the serially diluted plasma sample or recombinant bCgA protein generated in the present study. The assay sensitivity defined as the value of two standard deviations below the zero standard was calculated as 0.46 nM. The intraassay and interassay coefficients of variation were 6.48% and 13.4%, respectively. Changes in the plasma bCgA concentrations in early pregnancies undulated in nonpregnant animals. The results of the present study suggest that assaying plasma bCgA concentrations could be utilized as measures to evaluate the physiological status of cattle.  相似文献   

Lingual squamous cell carcinoma in a California sea lion (Zalophus californianus).     

Tsuneo Sato  Tomomi Higuchi  Hisashi Shibuya  Shigeo Ohba  Sadao Nogami  Wataru Shirai  Hideaki Watanabe  Shigeaki Honda 《Journal of zoo and wildlife medicine》2002,33(4):367-370

A 28-yr-old female California sea lion (Zalophus californianus) in a commercial aquarium developed an ulcerated lingual tumor and died. Necropsy revealed a moderately differentiated squamous cell carcinoma. Immunohistochemical staining and electron microscopic studies revealed that the tumor cells were strongly positive with anti-keratin-cytokeratin antibody and had abundant tonofibrils and desmosomes. The neoplasm had metastasized to a mandibular lymph node.  相似文献   

Gene expression profiles in bovine granulocytes reflect the aberration of liver functions     

Keiichiro Kizaki  Tomomi Kageyama  Noriyuki Toji  Katsuo Koshi  Kouya Sasaki  Norio Yamagishi  Toshina Ishiguro‐Oonuma  Toru Takahashi  Kazuyoshi Hashizume 《Animal Science Journal》2020,91(1)

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A case study of improving milking cow performance and milking system performance with using a flow simulator     

Masafumi Enokidani  Kazuhiro Kawai  Yasunori Shinozuka  Tomomi Kurumisawa 《Animal Science Journal》2020,91(1)

Efforts to improve dairy performance have been focused on increasing milk productivity of cows through improved feeding systems and genetic potential. However, methods for evaluating milking system performance based on milk productivity have not yet been established. Milking system performance was evaluated by measuring the claw vacuum at five flow rates (1.9–8.7 kg/min) produced using a flow simulator for a single eight‐swing milking parlor with a high‐line system. Based on these results, a double eight‐parallel milking parlor with a low‐line system was installed and tested. Farmers can take data obtained from evaluations of milking system performance into account for future management decisions, such as renewing the milking system. By renewing the milking system, average milking productivity, somatic cell linear score (LS) of bulk milk, and LS of each cow were significantly improved in the year after installing the new system (p < .01). In addition to checking conventional milking systems, this novel diagnostic method using a flow simulator can be used for checking new installations and also for proposing renovations.  相似文献   

In vitro culture of endosperm and its application in plant breeding: Approaches to polyploidy breeding     

Yoichiro Hoshino  Tomomi Miyashita  Thuruthiyil Dennis Thomas 《Scientia Horticulturae》2011

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Mercury Speciation in the Water of Minamata Bay, Japan     

Akito Matsuyama  Tomomi Eguchi  Ikuko Sonoda  Akihide Tada  Shinichirou Yano  Akira Tai  Kohji Marumoto  Takashi Tomiyasu  Hirokatsu Akagi 《Water, air, and soil pollution》2011,218(1-4):399-412

The speciation of mercury (Hg) in Minamata Bay (Japan) was studied over a 2-year period (2006?C2008). Concentrations of dissolved total Hg, dissolved methylmercury (MeHg), particulate total Hg, and suspended solids were 0.43?±?0.14 ng/l (mean?±?standard deviation), 0.10?±?0.06 ng/l, 3.04?±?2.96 ng/l, and 5.94?±?2.10 mg/l, respectively. Correlations between concentrations of particulate total Hg and suspended solids at four depths (surface: 0 m; mid-depth: ?6 m, ?10 m; and bottom +1 m layer) were only significant in the bottom +1 m layer. The mean dissolved MeHg concentration and the ratio of dissolved MeHg to dissolved total Hg were considerably higher in summer compared to other seasons. The data suggest that bottom sediment was not the sole source of MeHg, and that MeHg may be produced in the water column by the conversion of divalent Hg eluted from resuspended bottom sediment. The correlation between seawater characteristics such as salinity, temperature, dissolved oxygen (DO), and dissolved MeHg concentration indicates that Hg methylation could be influenced by the heterotrophic activity of microorganisms in the seawater. In particular, inverse correlations were observed between DO, salinity, and MeHg concentration. However, dissolved MeHg concentrations did not correlate with seawater characteristics such as pH or chlorophyll-a.  相似文献   

Insect growth regulating activity of 1,5-disubstituted imidazoles against Bombyx mori and Oncopeltus fasciatus     

Eiichi Kuwano  Tomomi Hisano  Morifusa Eto  Koichi Suzuki  Gopalan C. Unnithan  William S. Bowers 《Pest management science》1992,34(3):263-268

Seventeen 1,5-disubstituted imidazoles possessing a variety of substituents were tested on Bombyx mori and Oncopeltus fasciatus. All compounds induced precocious metamorphosis from 4th-instar larvae of B. mori. Of the compounds tested, 1-benzyl-5-[(E)-2,6-dimethyl-1,5-heptadienyl]imidazole (KK-42) was the most active against B. mori larvae. However, none of the compounds induced precocious metamorphosis in O. fasciatus nymphs, although several showed delayed toxicity. Treated nymphs died during or immediately after the moult. A series of 1-isobutylimidazoles exhibited high activity against O. fasciatus, whereas KK-42 was inactive even at high doses. There was no apparent correlation between the ability of imidazoles to cause precocious metamorphosis in B. mori and the delayed toxicity for O. fasciatus.  相似文献   

Monitoring of the in situ growth of three <Emphasis Type="Italic">Acropora</Emphasis> corals by individual identification over 5 years at the northern outer reef of Sekisei Lagoon (24°N), Okinawa     

Mineo Okamoto  Satoshi Nojima  Shuichi Fujiwara  Tomomi Saito 《Fisheries Science》2016,82(6):905-913

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Ruminal fermentation and microbial ecology of buffaloes and cattle fed the same diet     

Khin‐Ohnmar LWIN  Makoto KONDO  Tomomi BAN‐TOKUDA  Rosalina M LAPITAN  Arnel N. DEL‐BARRIO  Hiroki MATSUI 《Animal Science Journal》2012,83(12):767-776

Although buffaloes and cattle are ruminants, their digestive capabilities and rumen microbial compositions are considered to be different. The purpose of this study was to compare the rumen microbial ecology of crossbred water buffaloes and cattle that were fed the same diet. Cattle exhibited a higher fermentation rate than buffaloes. Methane production and methanogen density were lower in buffaloes. Phylogenetic analysis of Fibrobacter succinogenes‐specific 16S ribosomal RNA gene clone library showed that the diversity of groups within a species was significantly different (P < 0.05) between buffalo and cattle and most of the clones were affiliated with group 2 of the species. Population densities of F. succinogenes, Ruminococcus albus and R. flavefaciens were higher until 6 h post‐feeding in cattle; however, buffaloes exhibited different traits. The population of anaerobic fungi decreased at 3 h in cattle compared to buffaloes and was similar at 0 h and 6 h. The diversity profiles of bacteria and fungi were similar in the two species. The present study showed that the profiles of the fermentation process, microbial population and diversity were similar in crossbred water buffaloes and crossbred cattle.  相似文献   

Genome‐wide association studies reveal additional related loci for fatty acid composition in a Duroc pig multigenerational population          下载免费PDF全文

Shuji Sato  Yoshinobu Uemoto  Takashi Kikuchi  Sachiko Egawa  Kimiko Kohira  Tomomi Saito  Satoshi Miyashita  Shinji Arata  Keiichi Suzuki 《Animal Science Journal》2017,88(10):1482-1490

The aim of the present study was to detect quantitative trait loci affecting fatty acid composition in back fat and intramuscular fat in a Duroc pig population comprising seventh‐generation pedigrees using genome‐wide association studies (GWAS). In total, 305 animals were genotyped using single nucleotide polymorphisms (SNPs) array and five selected SNPs from regions containing known candidate genes related to fatty acid synthesis or metabolism. In total, 24 genome‐wide significant SNP regions were detected in 12 traits, and 76 genome‐wide suggestive SNP regions were detected in 33 traits. The Sus scrofa chromosome (SSC) 7 at 10.3 Mb was significantly associated with C17:0 in intramuscular fat, while the SSC9 at 13.6 Mb was significantly associated with C14:0 in intramuscular fat. The SSC12 at 1.0 Mb was significantly associated with C14:0 in back fat and the SSC14 at 121.0 Mb was significantly associated with C18:0 in intramuscular fat. These regions not only replicated previously reported loci containing some candidate genes involved in fatty acid composition (fatty acid synthase and stearoyl‐CoA desaturase) but also included several additional related loci.  相似文献