Nitrogen Retention in Japanese Cedar Stands in Northern Honshu, with High Nitrogen Deposition   总被引：1，自引：0，他引：1  

M. Baba  Y. Suzuki  H. Sasaki  K. Matano  T. Sugiura  H. Kobayashi 《Water, air, and soil pollution》2001,130(1-4):1103-1108

High nitrogen, especially ammonium, input has been observed in Schichinohe, Aomori Prefecture, northeastern Japan. A monitoring study on precipitation, throughfall, and stream water has been carried out to estimate the stage of nitrogen saturation since 1996. Fifty-two to 70% of nitrogen input in throughfall was retained in forest ecosystems. Nitrate concentration in stream water tended to decrease throughout the study. There was no symptom of nitrogen saturation at Japanese cedar stands in Shichinohe, although high nitrogen input in open bulk has been observed. Ammonium (NH₄ ⁺) was retained in the canopy. The ratio of NH₄ ⁺ input in throughfall to that by open bulk was 0.40 – 0.47. Total inorganic nitrogen input under the canopy amounted 0.68 – 0.72 kmolc ha^?1 yr^?1 (9.6 – 10.0 kg N ha^?1 yr^?1). Our results suggests that atmospheric nitrogen input has benefitted the three growth.  相似文献   

Effects of Rice Flour Blends on Bread Texture and Staling     

Tomoko Sasaki  Kaoru Kohyama  Kanae Miyashita  Tomoya Okunishi 《Cereal Chemistry》2014,91(2):146-151

The effects of blending rice flour with wheat flour on bread texture and staling were investigated with three rice varieties with different amylose contents. For the texture analysis of bread crumb, the compression test, puncture test, and tensile test were performed. A flour blend containing rice flour suppressed the recovery of the crumb after compression. For the puncture test, blends with rice flour increased the distance to penetrate the crumb. The rupture strain measured with the puncture test decreased with staling of the bread crumb for all samples tested. The fresh bread crumb sample containing waxy rice flour had much greater extensibility in the tensile test than the other samples tested, but it was dramatically decreased after one day of storage. Endothermic enthalpies corresponding to retrograded amylopectin, which is part of the staling process, were also measured. The enthalpy of bread crumb from the blended flour was lower than that of wheat flour bread up to three days but was higher on day 4. A blend of rice flour thus reduced amylopectin retrogradation during early storage, but it was accelerated later. Bread blended with waxy rice flour showed the lowest enthalpy during storage.  相似文献   

Results of hyperamplification of centrosomes in naturally developing tumors of dogs.     

A Setoguchi  M Okuda  E Nishida  M Yazawa  T Ishizaka  S H Hong  M Hisasue  R Nishimura  N Sasaki  Y Yoshikawa  K Masuda  K Ohno  H Tsujimoto 《American journal of veterinary research》2001,62(7):1134-1141

OBJECTIVE: To evaluate results of centrosome hyperamplification in naturally developing tumors of dogs. SAMPLE POPULATION: Tumor specimens from 9 dogs with tumors (rhabdomyosarcoma, osteosarcoma, chondrosarcoma, myxosarcoma, and mammary gland tumor) and 2 canine osteosarcoma cell lines. PROCEDURE: 3 antibodies for centrosome proteins (ie, anti-gamma-tubulin, anti-BRCA1, and anti-pericentrin) were used for immunohistochemical analysis. Double immunostaining for centrosomes was used to confirm the specificity of these antibodies for centrosomes. Mutational analysis of the canine p53 gene was carried out by polymerase chain reaction-single-strand conformation polymorphism analysis, and expression of canine MDM2 protein was evaluated by use of immunohistochemical analysis, using anti-MDM2 antibody. RESULTS: Immunohistochemical analysis of dog osteosarcoma cell lines with apparent aneuploidy revealed frequent hyperamplification of centrosomes in the osteosarcoma cell lines. Similar hyperamplified centrosomes were detected in the tumor tissues from all of the 9 tumors. The frequency of cells with hyperamplified centrosomes (3 to 20/cell) in each tumor tissue ranged from 9.50 to 48.1%, whereas centrosome hyperamplification was not observed in normal lymph nodes from these dogs. In 8 of the 9 tumors, mutation of p53 gene or overexpression of MDM2, or both, was detected. CONCLUSIONS AND CLINICAL RELEVANCE: Various types of naturally developing tumors in dogs often have hyperamplification of centrosomes associated with chromosome instability. Hyperamplification of centrosomes is a novel tumor marker for use in cytologic and histologic examinations of clinical specimens obtained from dogs.  相似文献   

Effects of genotype matching of feline major histocompatibility complex (FLA) class II DRB on skin-allograft transplantation in cats.     

Y Kuwahara  K Kito  R Kobayash  J Iwata  R Ohne  H Kitagawa  Y Sasaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1097-1101

In order to confirm the effects of matching of expressed feline major histocompatibility complex (FLA) class II DRB genotype on transplantation immunity in cats, skin-allogeneic transplantation was carried out between cats, in which DRB genes expressed were genotyped by the RT-PCR-RFLP method using group-specific primers. Duration until grafts were rejected was 14.63 +/- 1.69 days (mean +/- standard deviation) in the pairs that had the same type of subgroups, 7.25 +/- 0.71 days in the pairs that had one different type of subgroup and 6.88 +/- 0.35 days in the pairs that had two different types of subgroups. The duration of graft survival in the pairs with the same type of subgroups was significantly longer (P<0.01) than those in the pairs with different types. Although FLA components involved in transplantation immunity should not only be DRB genes, it was suggested that the expressed FLA-DRB genotype might associate with feline transplantation immunity, and that typing and matching of expressed FLA-DRB genes might be one of the important factors in the control of feline transplantation immunity.  相似文献   

CDNA cloning of feline leptin and its mRNA expression in adipose tissue.     

N Sasaki  H Shibata  T Honjoh  K Kimura  M Saito  I Ohishi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1115-1120

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Protection from pseudorabies virus challenge in mice by a combination of purified gII, gIII and gVI antigens.     

A Matsuda Tsuchida  S Katayama  N Okada  T Okabe  N Sasaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(3):447-452

The antigens gII, gIII and gVI were purified from the lysates of the pseudorabies virus (PRV) -infected HmLu-1 cells using Sepharose 4 B coupled with MAbs against these antigens. Mice immunized with either gII, gIII, gVI antigen or a mixture of them were challenged intraperitoneally with 8.5 x 10(3) plaque forming units of PRV. All the mice immunized with 1.5 and 4.5 micrograms of the mixture and 4.5 micrograms of the gIII antigen survived. The sera of mice immunized with the mixture had virus neutralizing activity which was independent of complement, hemagglutination inhibition activity, and recognized major (93 kilodaltons) and minor (129, 74, 68 and 50 kilodaltons) PRV proteins under reducing conditions in western blotting. The serological activity levels in the lower survival group were not different from those in the complete survival. These results indicate that the mixture of each glycoprotein is more effective for eliciting of protective immunities in mice and that serological activity do not always correlate with protection.  相似文献   

Spongiform encephalopathy in sheep scrapie: electron microscopic observations     

S Sasaki  S Mizoi  A Akashima  M Shinagawa  H Goto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(4):791-796

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Detection of uremic peaks in dogs by anion exchange high performance liquid chromatography.     

M Kawamura  F Ohashi  F Murakami  N Takai  R Nishimura  N Sasaki  A Takeuchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):951-955

Sera of dogs with gentamicin-induced uremia were analyzed by high performance liquid chromatography system with strongly basic macroreticular anion exchange resin. Satisfactory separation of peaks was achieved with good reproducibility after deproteinization of sera with trichloroacetic acid at a final concentration of 3%, confirming that the system was suitable for qualitative analysis of uremic serum. The chromatograms showed that the number of peaks and the peak area had relation to concentrations of serum urea nitrogen or creatinine and severity of uremia. Four peaks were selected as suspected canine uremic peaks with high correlation to serum creatinine concentrations which were hardly influenced by extrarenal factors. The results suggested that these four fractions might contain uremic toxins.  相似文献   

Tumourigenesis by partial body X-irradiation in mice     

F Sato  S Sasaki  F Chino  D Endoh 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(6):1161-1168

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Antibody-dependent adherence of guinea pig eosinophils to the third-stage larvae of Metastrongylus apri     

O Sasaki  M Katsuno 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(3):623-627

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