Cultivation Practices and Potato Cultivars Suitable for Organic Potato Production     

Jannie Elisabeth Hagman  Anna Mårtensson  Ulf Grandin 《Potato Research》2009,52(4):319-330

To identify the most important agronomic measures and cultivar traits in Swedish organic potato production, multivariate analyses were performed on a set of data from a series of field trials carried out in Sweden during a 7-year period. The effects of soil parameters, cultivar, year and geographical location on potato characteristics were investigated. Soil parameters including fertility level had strong and significant effects on potato characteristics, explaining 53% of total variation. Variables related to the duration of haulm growth were other dominant factors in the variation. While P and K fertilization increased yields, N fertilization had little effect on yield and a negative effect on the time to emergence. The N requirement of potatoes ranges from 2.5 to 5.9 kg ha^?1 per ton of tuber yield and was met in these trials. The results implied the timing of N application is important for yield. It was concluded that the importance of P and K fertilization is underestimated in organic production, and that the most important cultivar trait in achieving acceptable yield is long-lasting foliage, which is a characteristic of cultivars resistant to Phytophthora infestans. Three of the cultivars tested (Lady Balfour, Cicero and Sarpo Mira) had a yield >3.5 kg m^?2, which is high in Swedish organic production.  相似文献   

Reproductive performance in sows in relation to Japanese Encephalitis Virus seropositivity in an endemic area     

Lindahl J  Boqvist S  Ståhl K  Thu HT  Magnusson U 《Tropical animal health and production》2012,44(2):239-245

Japanese Encephalitis Virus (JEV) is considered an important reproductive pathogen in pigs. Most studies of the reproductive impact of JEV have been conducted in areas where the disease occurs in seasonal epidemics. In this study, the associations between seropositivity for JEV, measured with an IgG ELISA, and the number of piglets born alive and stillborn were investigated in a tropical area endemic for JEV in Vietnam. Sixty percent of sows from four farms in the Mekong delta of Vietnam were seropositive to JEV and the Odds Ratio for a sow being infected was highest (6.4) in sows above 3.5 years (95% confidence interval 2.2–18.3). There was an association between increasing Optical Density (OD) values from the ELISA and the number of stillborn piglets in sows less than 1.5 years, but no effect of seropositivity could be shown when all sows were studied. OD values had an effect (p = 0.04) on the number of piglets born alive in the statistical analysis only when interacting with the effect of the breeds. An increase in mean OD value of the herd was correlated (p < 0.0001) with an increase in the number of piglets born alive. In this study, there was evidence of a negative association between seropositivity for JEV and the reproductive performance only in sows less than 1.5 years in endemic areas. This could be explained by a year-round infection with the virus, which would lead to immunity in many gilts before their first pregnancy. This, in turn, may imply that JEV infection in pigs is of minor importance for the reproductive performance in endemic areas.  相似文献   

The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus—Just a False Positive?     

Malin Strand  Martin Hedstr?m  Henrik Seth  Eric G. McEvoy  Erik Jacobsson  Ulf G?ransson  H?kan S. Andersson  Per Sundberg 《Marine drugs》2016,14(4)

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel–dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.  相似文献   

Clinical pharmacology of methadone in dogs     

Carina Ingvast-Larsson  Anja Holgersson  Ulf Bondesson†  Anne-Sofie Lagerstedt‡  & Kerstin Olsson§ 《Veterinary anaesthesia and analgesia》2010,37(1):48-56

ObjectiveTo investigate the pharmacokinetics and effects of methadone on behaviour and plasma concentrations of cortisol and vasopressin in healthy dogs.Study designRandomized, cross-over, experimental trial.AnimalsNine adult dogs (beagle and beagle cross breeds), four males and five females.MethodsMethadone hydrochloride, 0.4 mg kg^?1, was administered intravenously (IV) and subcutaneously (SC) with a crossover design. Drug and hormone analyses in plasma were performed using Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry and radioimmunoassay respectively. Behavioural data were collected using a standardized protocol.ResultsAfter IV administration, the plasma concentration of methadone at 10 minutes was 82.1 ± 9.2 ng mL^?1 (mean ± SD), the terminal half-life was 3.9 ± 1.0 hours, the volume of distribution 9.2 ± 3.3 L kg^?1 and plasma clearance 27.9 ± 7.6 mL minute^?1 kg^?1. After SC administration, time to maximal plasma concentration was 1.26 ± 1.04 hours and maximal plasma concentration of methadone was 23.9 ± 14.4 ng mL^?1, the terminal half-life was 10.7 ± 4.3 hours and bioavailability was 79 ± 22%. Concentrations of both cortisol and vasopressin were increased for an hour following IV methadone. The observed behavioural effects of methadone were decreased licking and swallowing and an increase in whining after SC administration. The latter finding is notable as it can be misinterpreted as pain when methadone is used as an analgesic.Conclusion and clinical relevanceWhen methadone was administered by the SC route, the half-life was longer, but the individual variation in plasma concentrations was greater compared with IV administration. Increased frequency of whining occurred after administration of methadone and may be a drug effect and not a sign of pain. Cortisol and vasopressin concentrations in plasma may not be suitable for evaluating analgesia after methadone treatment.  相似文献   

Water distribution in brine salted cod (Gadus morhua) and salmon (Salmo salar): a low-field 1H NMR study     

Aursand IG  Gallart-Jornet L  Erikson U  Axelson DE  Rustad T 《Journal of agricultural and food chemistry》2008,56(15):6252-6260

Low-field (LF) (1)H NMR T 2 relaxation measurements were used to study changes in water distribution in lean (Atlantic cod) and fatty (Atlantic salmon) fish during salting in 15% NaCl and 25% NaCl brines. The NMR data were treated by PCA, continuous distribution analysis, and biexponential fitting and compared with physicochemical data. Two main water pools were observed in unsalted fish, T 21, with relaxation times in the range 20-100 ms, and T 22, with relaxation times in the range 100-300 ms. Pronounced changes in T 2 relaxation data were observed during salting, revealing changes in the water properties. Salting in 15% brine lead to a shift toward longer relaxation times, reflecting increased water mobility, whereas, salting in saturated brines had the opposite effect. Water mobility changes were observed earlier in the salting process for cod compared to salmon. Good linear correlations ( F 相似文献   

Quantification of infiltration processes in urban areas by accounting for spatial parameter variability     

Ulf Mohrlok  Leif Wolf  Jochen Klinger 《Journal of Soils and Sediments》2008,8(1):34-42

Background, Aims, and Scope  As a consequence of human living and activity, water infiltration to the urban subsurface occurs from a variety of different sources, like precipitation, irrigation, leaking pipes and sewers, septic tanks and rainwater infiltration ponds. This infiltration is strongly related with quality issues of the infiltrated water and further impact on groundwater quality. In order to set up an integrated urban water balance it becomes essential to estimate the infiltration processes, i.e. water flow and solute transport, from these different infiltration sources and to take into account the large spatial variability of sediment properties, the geometric settings of these sources and the groundwater table. For that purpose, the development of simple, physically-based quantification approaches is required in order to establish an efficiently working prediction and risk analysis tool within the framework of an integrated urban water management system. The scope of the presented work was to demonstrate the applicability of the developed approaches at urban scale. Methods  Since a detailed, three-dimensional, numerical quantification of the infiltration processes within the entire urban area is not possible, the individual sources were considered as independent within the EU AISUWRS project. Different models were developed for balancing infiltration from areal and point sources with respect to the related flow pattern. The analytical model UL_FLOW, based on one-dimensional, steady state analytical solutions, allows the estimation of conservative tracer residence times in layered sediments under varying infiltration rates. The numerical model WSTM, based on a three-dimensional random walk approach, calculates water and solute transport from pipe leaks. Additionally, the sources were classified in accordance to the spatial distribution of the parameters determining the infiltration processes. Results  UL_FLOW was applied to data sets from the city of Rastatt within a case study of the AISUWRS project. Each neighbourhood of water balance computation by the Urban Volume and Quality Model (UVQ) was defined as an areal infiltration source with unique parameter values for sediment depth, profile and properties, as well as infiltration rate time series. Groundwater recharge and residence time series were computed for each neighbourhood. Relevant statistical parameters obtained by time series analyses from those time series could be mapped by GIS. Point infiltration, particularly from sewers, was classified due to the sediment parameters and the distance to the groundwater table at each source location in order to reduce computational efforts. WSTM computations provided time series of groundwater recharge and tracer breakthrough for some specific cases. Discussion  The analytical model UL_FLOW provides fast and efficient computation of groundwater recharge and residence times accounting for storage effects within the unsaturated zone of urban areas. The reliability of this model has been shown by cross validation with HYDRUS1D. Because of the high computational effort, WSTM could provide only short-term simulations for some specific parameter sets for which residence time estimates could be derived. Conclusions  UL_FLOW provides an analytical modelling tool for balancing one-dimensional areal infiltration and estimating residence times under varying conditions including spatial parameter variability. These balances could be used for assessing the impact of those infiltration sources on groundwater quality. The tracer breakthrough from point infiltration sources computed by WSTM could also be used for such kinds of assessment. The larger spatial parameter variability associated with these sources could be handled by classification in GIS environments. Recommendations and Perspectives  Similar to the areal sources, a simple balance approach for point sources based on analytical solutions needs to be developed for estimating residence times in order to avoid large computational efforts. Such a model would complete the balancing of all kinds of infiltration sources in urban areas efficiently. Since the approaches are based on the balance of the physical processes, they have a large predictive capability and could be included into an integrated urban water balance and management system. The mapping of the statistical values of the residence times provides a tool to compare parts of the urban areas and to visualize differences between urban water management scenarios.  相似文献   

Evolutionary impact assessment: accounting for evolutionary consequences of fishing in an ecosystem approach to fisheries management     

Ane T Laugen  Georg H Engelhard  Rebecca Whitlock  Robert Arlinghaus  Dorothy J Dankel  Erin S Dunlop  Anne M Eikeset  Katja Enberg  Christian Jørgensen  Shuichi Matsumura  Sébastien Nusslé  Davnah Urbach  Loїc Baulier  David S Boukal  Bruno Ernande  Fiona D Johnston  Fabian Mollet  Heidi Pardoe  Nina O Therkildsen  Silva Uusi‐Heikkilä  Anssi Vainikka  Mikko Heino  Adriaan D Rijnsdorp  Ulf Dieckmann 《Fish and Fisheries》2014,15(1):65-96

Managing fisheries resources to maintain healthy ecosystems is one of the main goals of the ecosystem approach to fisheries (EAF). While a number of international treaties call for the implementation of EAF, there are still gaps in the underlying methodology. One aspect that has received substantial scientific attention recently is fisheries‐induced evolution (FIE). Increasing evidence indicates that intensive fishing has the potential to exert strong directional selection on life‐history traits, behaviour, physiology, and morphology of exploited fish. Of particular concern is that reversing evolutionary responses to fishing can be much more difficult than reversing demographic or phenotypically plastic responses. Furthermore, like climate change, multiple agents cause FIE, with effects accumulating over time. Consequently, FIE may alter the utility derived from fish stocks, which in turn can modify the monetary value living aquatic resources provide to society. Quantifying and predicting the evolutionary effects of fishing is therefore important for both ecological and economic reasons. An important reason this is not happening is the lack of an appropriate assessment framework. We therefore describe the evolutionary impact assessment (EvoIA) as a structured approach for assessing the evolutionary consequences of fishing and evaluating the predicted evolutionary outcomes of alternative management options. EvoIA can contribute to EAF by clarifying how evolution may alter stock properties and ecological relations, support the precautionary approach to fisheries management by addressing a previously overlooked source of uncertainty and risk, and thus contribute to sustainable fisheries.  相似文献   

A macrophage factor inhibits adipocyte gene expression: an in vitro model of cachexia   总被引：64，自引：0，他引：64  

F M Torti  B Dieckmann  B Beutler  A Cerami  G M Ringold 《Science (New York, N.Y.)》1985,229(4716):867-869

Certain infections and malignancies in mammals cause the development of a condition known as cachexia in which the animal continues to lose weight, often while consuming an adequate diet. When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachectin, that inhibits the activity of fat-producing (lipogenic) enzymes in cultured adipocytes. This effect may reflect one of the physiological bases for cachexia. In the present study, clones of complementary DNA from genes whose expression is increased during the differentiation of adipocytes were used to study the molecular basis of cachectin's actions. In the presence of cachectin, the expression of the corresponding genes was reversibly and specifically inhibited. Furthermore, when mature adipocytes were exposed to cachectin, the messenger RNA's of those genes diminished and rapidly approached the levels present before differentiation.  相似文献   

Cetirizine in horses: pharmacokinetics and pharmacodynamics following repeated oral administration     

Olsén L  Bondesson U  Broström H  Tjälve H  Ingvast-Larsson C 《Veterinary journal (London, England : 1997)》2008,177(2):242-249

The pharmacokinetics of the histamine H(1)-antagonist cetirizine and its effect on histamine-induced cutaneous wheal formation were studied in six healthy horses following repeated oral administration. After three consecutive administrations of cetirizine (0.2 mg/kg body weight, bw) every 12h, the trough plasma concentration of cetirizine was 16+/-4 ng/mL (mean+/-SD) and the wheal formation was inhibited by 45+/-23%. After four additional administrations of cetirizine (0.4 mg/kg bw) every 12 h, the trough plasma concentration was 48+/-15 ng/mL and the wheal formation was inhibited by 68+/-11%. The terminal half-life was about 5.8 h. A pharmacokinetic/pharmacodynamic link model showed that the maximal inhibition of wheal formation was about 95% and the EC(50) about 18 ng/mL. It is concluded that cetirizine in doses of 0.2-0.4 mg/kg bw administered at 12 h intervals exhibits favourable pharmacokinetic and pharmacodynamic properties without causing visible side effects, and the drug may therefore be a useful antihistamine in equine medicine.  相似文献   

The milk delivery chain and presence of Brucella spp. antibodies in bulk milk in Uganda     

Kim Toeroek Rock  Denis Rwabiita Mugizi  Karl Ståhl  Ulf Magnusson  Sofia Boqvist 《Tropical animal health and production》2016,48(5):985-994

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